Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	342,510	C→G	12.2%	intergenic (+3/‑374)	yahJ → / → yahK	putative metallo‑dependent hydrolase domain deaminase/broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	342,510	0	C	G	12.2%	251.6 / 4.3	96	intergenic (+3/‑374)	yahJ/yahK	putative metallo‑dependent hydrolase domain deaminase/broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing
Reads supporting (aligned to +/- strand):  ref base C (27/55);  new base G (10/3);  total (38/58)
Fisher's exact test for biased strand distribution p-value = 4.42e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATCACACTTACAGGCGCTCGCCTTATGGGGAACGTT  >  NC_000913/342281‑342742                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           tttataTTCAGCCTAACGAACAGAACCTCTCCCGGTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGa                                                                                                                                                                                                                                   <  2:744695/236‑1 (MQ=255)     aatTCGTCCTAACGAACAGAACCTCCCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGc                                                                                                                                                                                                                              <  2:956047/238‑1 (MQ=255)      ttctgttcTAACGACCCGACCCTTCCCCGCTGGTTTTTTTTACCCCTGGGCGATCTATTGCCGGTGAATGAAAAAGGCGGTCGGGTA‑TGGCCAAAGGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGcc                                                                                                                                                                                                                             <  1:167653/232‑1 (MQ=255)        ttctCCTAACGAACAGAGCCTCGCCCGCCGGGTGTTTTTAGCCACTGGAGAGTTATGGCCGCCTAATGAAAAAGGGGAGCTGGGA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTg                                                                                                                                                                                                                           <  1:192166/236‑1 (MQ=255)        tCGTCCTTAAGAACAGACCCTCCCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGGGCGGGTA‑GGGCCAAAAGCCGAG‑GATGACGCCATCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTg                                                                                                                                                                                                                           <  1:224092/239‑1 (MQ=255)        tCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGAATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGGGG‑GGCAGGTTGAT‑TGGC‑ATTTGAAAAGCCTg                                                                                                                                                                                                                           >  2:995345/1‑239 (MQ=255)        ccGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTg                                                                                                                                                                                                                           <  2:383859/238‑1 (MQ=255)          gttcTAGCGAACCAACCCTCTCCCTCTCGCTGTTTTTAGCCACGGGCCATGTATTGCCGTGAAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAATCGCAG‑GATGCCGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGTTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCa                                                                                                                                                                                                                         <  1:393836/236‑1 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGCAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCCGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTCGGCCGTATCCCGCCGGGAAACGCAACGTTTCCTTAAGGGCCACTGGTGGGGGGGGAGGGT‑GCAGGGATGTT‑GCGC‑ATTGAAAACGCCGGc                                                                                                                                                                                                                          >  1:944636/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTTTCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑TGGGGGGGGGGG‑GGCAGGTTGAG‑GGGC‑ATTTGAAACGCCTGCa                                                                                                                                                                                                                         >  2:224092/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGCGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGG‑GGCAGGGTGAT‑GGGC‑ATTTGAAAAGCCTGCa                                                                                                                                                                                                                         >  2:192166/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGGGTGG‑GGCCAGTTGAT‑TCGC‑ATTTGAAACGCCTGCa                                                                                                                                                                                                                         >  2:770629/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCGGGTTGAT‑GCCC‑ATTTGAAACGCCTGCa                                                                                                                                                                                                                         >  2:1186840/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCa                                                                                                                                                                                                                         <  2:944636/239‑1 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGGTGAT‑GCGC‑ATTTGAAACGCCTGCa                                                                                                                                                                                                                         >  2:945507/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGG‑GTGGGGGAGTGT‑GGCAGGTTGAT‑TGGC‑ATTTTAAACGCCTCCa                                                                                                                                                                                                                         >  2:167653/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGG‑GGGGGGGGGGTG‑GGCAGGTTGAT‑CGGC‑ATTTGAAACGCCGGCa                                                                                                                                                                                                                         >  2:1017952/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGGATCTCGCCGGGGACCGCAACGTTCCCTAAAGGGCAACTGGG‑GTGGGGGGGTGT‑GGCAGGTTGAT‑GCGC‑CTTTGAAAAGCCCGCa                                                                                                                                                                                                                         >  2:987937/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGGGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGG‑GTGGGGGGGGGT‑GGCAGGGTTAG‑TGGC‑ATTTGAAAAGCCCGCa                                                                                                                                                                                                                         >  2:1266317/1‑239 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGGGGCGCCAAGCTCGCCGAGCACCGCAACGTTCCACAAAAGGCAAATGGA‑GGGGGGGGGGGG‑GGGAGGTGGAG‑GGGC‑AATTGAAAAGcgggca                                                                                                                                                                                                                         >  1:537971/1‑234 (MQ=255)          gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCAAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGGGGCGGGGGCGCGTATACTGCCCGGGACCCCCACGTTCCAAAAAGGGGAAGCGGG‑GGGGGGGGGGGG‑GGGAGGGTGAT‑GGGCGATTTaaaagaccgga                                                                                                                                                                                                                          >  1:383859/1‑232 (MQ=255)             cTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGG‑GTGGGGGGGGGT‑GGGAGGTTGAT‑GGGC‑ATTTGAAAACCCTGCAACg                                                                                                                                                                                                                      >  2:1217269/1‑239 (MQ=255)             cTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGGATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAAATGGG‑GTGGGGGGGTGT‑GGCAGGGTGGTGGCGC‑TTTGAAAACGctggaacg                                                                                                                                                                                                                       >  2:693292/1‑232 (MQ=255)                  gAACAGAACCTCGCCCGCTCGCTGTTTTTAGCCACGGGCGATGTATTGCCGCTGAATGAAAAAGGCGGGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGATAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAATCGCCTGCAACGGTGaa                                                                                                                                                                                                                 <  2:790455/239‑1 (MQ=255)                     cAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GGGGGGGAGGGG‑GGCAGGTTGAT‑GCGG‑ATTTGAAACGCCCGCAACGGGGatacg                                                                                                                                                                                                              >  2:897487/1‑235 (MQ=255)                             ctcCCGCTCGCTGTTTTTAGCCACTGGCGTTGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGGCCCTATTTCGCCGAGCAACGCAACGTTCCATACAGGGCCACTGGG‑TGGGGGGAGGGTGGGCAGGTTGTT‑G‑GC‑CATTGAAACGCCCCCAAAGGGGaaaaaacagagag                                                                                                                                                                                                      >  1:1076208/1‑228 (MQ=255)                             ctcCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGTT‑GCGC‑CTTTTAAACGCCTGCAACGGTGAATAATAAGAGAt                                                                                                                                                                                                      >  2:281390/1‑239 (MQ=255)                             ctcCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGGTGAT‑GCGC‑ATTTGAAACGGCTGCAACGGTGAATAGTAAgggaa                                                                                                                                                                                                      >  2:396374/1‑238 (MQ=255)                              tcCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGGGCATGGTaatggagt                                                                                                                                                                                                     >  2:96823/1‑233 (MQ=255)                               cccGCTGGCGTTTTTTAGCCACGGGCGATGTATTGCCGCTGAATGAAAAAGGCGGGTGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGAttt                                                                                                                                                                                                    <  2:135602/239‑1 (MQ=255)                               cccGCTCGGTGTTTTTAGCCATGGGCGATTGATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGAttt                                                                                                                                                                                                    <  2:1138759/239‑1 (MQ=255)                               cccGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGATGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGAttt                                                                                                                                                                                                    <  2:187150/239‑1 (MQ=255)                                ccGCTCCGTGTTTTTAGCCATTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTa                                                                                                                                                                                                   <  2:1076208/239‑1 (MQ=255)                                      gctGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACCGGT‑GTGGGGGAGGGG‑GGCAGGGTGAT‑GCGC‑ATTTGAAACGCCCGCCACGGGGGAAAGTAAGAGATTTAAGccc‑c                                                                                                                                                                                            >  2:492367/1‑239 (MQ=255)                                        ggTTTTTACCCCCTGGGGATTTATTGCACCGGAAAGAAAAAGGAGAGCGGGTA‑TGGCCAAAACTCGAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCCCCTGTTCCGCGGGGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑Ca                                                                                                                                                                                           <  1:1057084/237‑1 (MQ=255)                                          tttttAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGGGAACCGCAACGTTCCCTAAAGGGCAACTGGG‑GTGGGGGGGGGT‑GGCAGGTTGAT‑GGGC‑ATTTGAAAAGCCTTCAACGGGGAATAGTAAAAGATTTAAGCCC‑CAggg                                                                                                                                                                                        >  2:1116751/1‑239 (MQ=255)                                                  cACTGGCGTTGTATTGACGCGTAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCCG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATTTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTgggg                                                                                                                                                                                 <  2:801338/238‑1 (MQ=255)                                                  cACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTgggg                                                                                                                                                                                 <  2:32543/238‑1 (MQ=255)                                                   ctgggggttGTTTTGGCTCTGAGTGAAAAAGGGGGGCTGGTA‑TGGCCAAAAG‑GCAGTGATGACGCCTGCTTTGGGGTGGGGTACGGCTCCGTTTCCGCCGAGGCGGTGGGGCGTACTTCGGCGAGAACAGAAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCa                                                                                                                                                                               <  1:1218366/231‑1 (MQ=255)                                                   catggctgtgttttggcggagaatgaaAAAGGCGGGGGGTGA‑TGCCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCGGGTGTGCGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCa                                                                                                                                                                               <  1:50746/219‑1 (MQ=255)                                                    cTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGGGAACCGCAACGTTCCATCAAGGGCAACTGGT‑GGGGGGGGGGGT‑GGCAGGGTGAT‑GCGC‑CTTTTAAACACCTGCAACGGGGAATAGAAAGAGATTTAAACCC‑CCgggggggggggga                                                                                                                                                                              >  1:1262914/1‑236 (MQ=255)                                                    cTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGGTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGGGAATAGTAAGAGATTTAAGCCC‑CAGGGGGTGGGGccg                                                                                                                                                                              >  2:1057084/1‑237 (MQ=255)                                                     tGGCGATGTAGTGCCGCTGAATGAAAAAGGCGGGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAAt                                                                                                                                                                             <  2:647601/239‑1 (MQ=255)                                                     gggCGATGTATTGCCGCTGAATGAAAAAGGCGGGGCGGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAAt                                                                                                                                                                             <  2:1135412/238‑1 (MQ=255)                                                        gggggttttGCCGCTGAAGAAAAAAGGGGAG‑GGGTATTGGCCAAAAGCGCGG‑GATTGCGCCAGTTTTGGGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCt                                                                                                                                                                           <  1:1017952/231‑1 (MQ=255)                                                        cGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGGA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCt                                                                                                                                                                           <  2:1262914/238‑1 (MQ=255)                                                             tATTGCCGATGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAAt                                                                                                                                                                             <  2:1194331/231‑1 (MQ=255)                                                                     cTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGGGGCGCGAATCTTGCCGAGAACCGCAACCTTACATAAAGGGGAACGGGG‑GGGGGGGGGAGT‑GGCAGGGTGATCGCCC‑ATTTAACAGGCGACAAGGGGTGAAT‑GTAAGAGATTTAAAGCC‑CAggggggggggacatagtaaaataaggaggc                                                                                                                                                             >  1:40189/1‑219 (MQ=255)                                                                       gaatgaaAAAGGCGAGCGGGTA‑TGGCCAAAAGCGCCG‑GATGACGCCAGCTTGGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGAGGGTCTCTCGCCGAGAACCGCAACGTTCCCAAAAGGCCACCTGGT‑GTGGGGGGGTGT‑GGCAGGTTGGA‑GCCG‑ATTTTAAACGTCAGCAACGGCGGATAGTAAACGAGTTAAACCA‑CAGGGAgggggggcaaaaggatagagggtggc                                                                                                                                                             >  1:1194331/1‑217 (MQ=255)                                                                             aaaaGGCCAGCGTGTA‑AGGACAAAAGGGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGaa                                                                                                                                                     <  2:1283523/239‑1 (MQ=255)                                                                              aaaGTCGAGCGTGTA‑AGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGGCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCACCGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGATTATGAGTGTGTCGGCAGaaa                                                                                                                                                    <  2:955895/239‑1 (MQ=255)                                                                                     aGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATTCCGCCGAGGACCGAAACGTTCCATAACGGGCAACGGTG‑GTGGGGGATGGG‑GGCATGGTAGT‑GCGC‑CTTTGAAAGACCTGTAACGGGTGGTGGTAAGAGTATTAAGCCCTCGGGGAG‑GGGGAAACGTGAAAATGaagagggcagttgaaaaagcact                                                                                                                                             >  1:772638/1‑217 (MQ=255)                                                                                      tcGTGTT‑GGGCCAAAAGCGGAG‑GAGGGGGCCGGGTTTTGGCTGGTGGACGCCTCCTGTTCCCGCGAGGGGGTGGCGGGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACAt                                                                                                                                             <  1:1186840/237‑1 (MQ=255)                                                                                      gggtgtA‑TGCCCAAAAGCGACG‑TATGTCGCCAGCTTTGTGTGTGGGGTCGCCGTTGTTTCCGCAGAGGCGGTGGGGCGTATCTCGCCGAGAACCTCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑TGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACAt                                                                                                                                             <  1:804711/236‑1 (MQ=255)                                                                                       ggttgtgcgtgcAAAAGCGGGG‑GAGGACGCCAGGTTTGTGCCGGTGGACGGCTCCTGTTCCGGCGAGGCGGTGGGTCGTAACTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACGGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACAt                                                                                                                                             <  1:7713/227‑1 (MQ=255)                                                                                       ggggtttgggCCAAAAGCGCGG‑GGTCCCGGCAGCTTTGGGTTGGGTGACGCCTCCTGTTCCGCCGAGGCGTGGGCGGTGATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACAt                                                                                                                                             <  1:679156/230‑1 (MQ=255)                                                                                              tGGCCAAAAGGCGAG‑GATGGCGCCAGGTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTg                                                                                                                                     <  2:27342/239‑1 (MQ=255)                                                                                              tGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTg                                                                                                                                     <  2:594555/239‑1 (MQ=255)                                                                                              tGGCAAAAAGGGCAG‑GGTGCGGCCAGTTTTGTGCTGGGGGACCCCTCCTGTTCCGGCGAGGCGGTGGCGCGTATCGCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTg                                                                                                                                     <  1:1217269/239‑1 (MQ=255)                                                                                                gCCAAAAGCCGCG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAAt                                                                                                                                                                             <  2:33143/197‑1 (MQ=255)                                                                                                     aaGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAata                                                                                                                              <  2:1215221/239‑1 (MQ=255)                                                                                                        gggaG‑GTTGACGCCCGTTTTGTGGTGGTGGACGCCTCCTGTTCCGGCGAGGCGGTGGCGGGGATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATg                                                                                                                            <  1:53359/235‑1 (MQ=255)                                                                                                          cAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGGGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAAAGGGCATTATCTTTGTGGAATATga                                                                                                                           >  2:376833/1‑236 (MQ=255)                                                                                                          cAG‑GAGGACGCCAGCTTTGTGCTGGGGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGcc                                                                                                                          <  2:1019234/238‑1 (MQ=255)                                                                                                              gATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACAt                                                                                                                                             <  2:534983/216‑1 (MQ=255)                                                                                                                    gCCAGCTTTGTGCTGGTGGCCGCCTCCTGTTCCCCCGAGGCGGTGGCGCGTATCGCGGCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAAt                                                                                                                                                                             <  1:856176/178‑1 (MQ=255)                                                                                                                        tgtttGGGCGGGGGGACGCCCCCTGTTCCGCGGAGGCGGGGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTtgt                                                                                                            <  1:1216251/237‑1 (MQ=255)                                                                                                                        gCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGGGAATAGTAAGAGAGTTAAGCCC‑CCGGGAGTGGGGCAATCTGAATAAGAGGGTTTGGGGAGAAAAAGACAATATATTTGAgggcggttcttaaaaaaaacggac                                                                                                            >  1:534983/1‑217 (MQ=255)                                                                                                                         cTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTaa                                                                                                                                                                                                           <  2:86293/144‑1 (MQ=255)                                                                                                                             gtgCTGGTGGACGCCTTCTGTTCCGCCGGGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGa                                                                                                       <  2:537971/239‑1 (MQ=255)                                                                                                                              tgCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTTGCGCGTATCTCGGCGAGGACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGAt                                                                                                      <  2:610601/239‑1 (MQ=255)                                                                                                                              tgCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAAAATGa                                                                                                                                                                   >  2:856176/1‑178 (MQ=255)                                                                                                                               gCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGAt                                                                                                      <  2:685568/238‑1 (MQ=255)                                                                                                                                             cctGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTTTCTTTGAGGAAAATGCCCTTATTTCCGTTTTGTAGGTAACTATTTTTCTACg                                                                                       >  2:907617/1‑239 (MQ=255)                                                                                                                                             cctGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGGGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGGGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACg                                                                                       >  2:129911/1‑239 (MQ=255)                                                                                                                                                 ttCCCCCGGGGCGGTGGCGGTTTTCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGaa                                                                                   <  1:987937/239‑1 (MQ=255)                                                                                                                                                                  cgcgtttcttcgcGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTg                                                                   <  2:40189/226‑1 (MQ=255)                                                                                                                                                                        tctcGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGAttt                                                            <  2:651933/239‑1 (MQ=255)                                                                                                                                                                           cGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAagag                                                                                                                                                                                                        >  2:928374/1‑97 (MQ=255)                                                                                                                                                                                      ggaaCGTTCCATAAAGGGCAACTGGT‑GGGGGGGAGTTT‑GGCAAGGTGAT‑GGCC‑ATTTGAAACGCCTGCAACGGTAAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTTAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGa                                              <  1:96823/237‑1 (MQ=255)                                                                                                                                                                                          cGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGc                                          <  2:734978/239‑1 (MQ=255)                                                                                                                                                                                          agTTCCATAAAGGGCAACGGGT‑GGTGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGc                                          <  2:198779/238‑1 (MQ=255)                                                                                                                                                                                            ttCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAC‑GCGC‑ATTTGAAACGCCTGCAACGGGGAATAGTACGAGGTTTAAGCCC‑CAAGGAGAGGGGCAATACGAATATGAGTGGGACGGCAGAAAGGGACATTATCTTTGAGGAAAATGCCCTTATTGACGTTGTGTAGAAAACAATTTGTCTCCGTGAAAAGACATTCACTTTTACCGCGTTTaaatttttaagacaagcaa                                        >  1:1041307/1‑221 (MQ=255)                                                                                                                                                                                              ccataagtggcactgggT‑GTGGGGGGTTGT‑GTCAGGTTTAT‑GGGC‑AGTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTTTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCTGATTTTAGTCTTTTACAGATAGCAAat                                      <  1:1056524/224‑1 (MQ=255)                                                                                                                                                                                               cttAAAGGGTCATTGGG‑GGGGGGGAGTGT‑GGCAGGTTGAT‑GCCC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAata                                     <  1:129911/237‑1 (MQ=255)                                                                                                                                                                                                 tAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATc                                   <  2:673729/239‑1 (MQ=255)                                                                                                                                                                                                      ggCAACTGGT‑GTGGGGGAGTGG‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTAACGTTGTGTAGAt                                                                                                      >  2:873128/1‑167 (MQ=255)                                                                                                                                                                                                                ggtgGGGGTGGGT‑‑GCAGGTTTAT‑GCGA‑ATTTTGAACTGCTGCAACGGTGAATATGAAGAGATTTAAGCCC‑CGGGGAGTGGGGCAATCTGAATATGATTGTGTCGGCAGAAATGTACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATCACACTTACAGGCGCt                    <  1:770629/238‑1 (MQ=255)                                                                                                                                                                                                                 gtgGGGGGGTGT‑GGTAGGTTGAT‑GCGC‑CTTTGAAACGGCGGCAACGGTGAATTGTAAGAGATTTAAGCCC‑CAGGGATTGGGGCAATGTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATCACACTTACAGGCGCt                    <  1:995345/239‑1 (MQ=255)                                                                                                                                                                                                                  tgGGGGGGTGT‑GGCAGGTTGAG‑TGGC‑CTTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATCACACTTACAGGCGCTc                   <  1:1266317/239‑1 (MQ=255)                                                                                                                                                                                                                  tgGGGGAGTTT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATCACACTTACAGGCGCTc                   <  2:381664/239‑1 (MQ=255)                                                                                                                                                                                                                  gtggggAGTGT‑TGCAGGTTTGT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTAGCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATCACACTTACAGGCGCt                    <  2:414255/236‑1 (MQ=255)                                                                                                                                                                                                                    ggggAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGGAGATAACTATTTGTCTACGTGAAAAGATCATCAGATTTGCCGCTTTTAGTCTTTTACAGATAGCCAATATCACACTTAAAGGCGCTCg                  >  2:132886/1‑238 (MQ=255)                                                                                                                                                                                                                          tgt‑gGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATTAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCCTCAGTTTTTGCGATTTTAGTTTTTTTAAAAAAGAAAAAATCAAACTTACAGGGCCTCGACtaaag           >  1:1131378/1‑235 (MQ=255)                                                                                                                                                                                                                               gCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATCACACTTACAGGCGCTCGCCTTATgggg        <  2:1019128/238‑1 (MQ=255)                                                                                                                                                                                                                                    ttGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGTTAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTCCAGATAGCAAATATCAAACTTCCATGCGCACCCCTTTTGGGGAACGtt  >  2:1094189/1‑239 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           TCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTA‑TGGCCAAAAGCGCAG‑GATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGT‑GTGGGGGAGTGT‑GGCAGGTTGAT‑GCGC‑ATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCC‑CAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAGATAGCAAATATCACACTTACAGGCGCTCGCCTTATGGGGAACGTT  >  NC_000913/342281‑342742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑