| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,016,069 | A→G | 15.5% | S34P (TCT→CCT) | ysgA ← | putative carboxymethylenebutenolidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,016,069 | 0 | A | G | 15.5% | 153.5 / 3.9 | 72 | S34P (TCT→CCT) | ysgA | putative carboxymethylenebutenolidase |
| Reads supporting (aligned to +/- strand): ref base A (50/9); new base G (4/8); total (55/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTATGTGATTTGCATCACTTT > NC_000913/4015843‑4016306 | cTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGGGGTGAAGCCCg > 2:877186/1‑238 (MQ=255)cTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGAAATGTTCATGCCCGCCAAAAATTT‑CCTGCACTACAATGACCACTAGCAGTGTGCCA‑TCCCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTAACCCCTTGTGAAGGGGTAGGGGGGAAGgcccc > 1:1277818/1‑237 (MQ=255) cggggggATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAACGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGTCAAAAATTT‑CCGACACTACAAGGACAACTGGACGTGGGCCC‑TCCCCTTGCTTTGGACGTGCATTGTAAACAGCCATG‑TTATCCCCTTTGGAATGGTAAGAGGTGTAGCGCGcccaaaccc > 1:135871/4‑232 (MQ=255) cGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTTTGAAGGGATAGAGGGGAAGCCCGCCACAATc > 2:426400/1‑238 (MQ=255) gcggggATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTATAGTACAGGTGCGATATCCAGATCCCCCTCCAGCGCCAGACGACGACAAATGTCGCGGCTATGTTCATGCAAGCCCGAAATTT‑CCTGCACGACAATGCACACTGGCAGTGGGCCA‑TCGCTTTTCTTTTGTCTGGCATGGTAAGACTGACTG‑TTCTCCCCTCCGGAGGCGATATAGGTGacgaccccaccacacgc > 1:133353/3‑223 (MQ=255) tCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCCCTTTGCTTTGGTCTGGCATGGTAAGCAGGCCTG‑TATCCCCCTTGTGAAGGGAAAAAAGGGAAGCACGcacaaaacgcgtgtcccgg > 1:497534/1‑221 (MQ=255) ccTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGc > 2:1273772/1‑239 (MQ=255) cTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGGTGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTTGGAAGGGTTAGAGGGTAAGCCCGCCACAATCGCGTCGTCCCGGGTCTGAAAGATGGTCGAAACg > 1:850251/1‑239 (MQ=255) cgcgGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCCCAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCggga > 2:945700/1‑239 (MQ=255) cAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGCCTGAACGATGGCCGAAGCGAGAGGCGTTGCAGCAGGt > 2:1051721/1‑239 (MQ=255) cAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGCCTGGCATGGTAAGCAGGCACG‑TTATCCCCTTTTGAAGGGATAGGGTGGAAGCCCGCCACAAGCGCGGCGCCCGGGGCCTGAAGTATGGTGGAAGCgcggggacacgaaagaggt > 1:854045/1‑221 (MQ=255) aTAGCCATATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACACCAAAAATTT‑CCTGCACTACAATGACCACTGGCCGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑CTTTCCCCTTTTGAAGGGATAGAGGGGAAGCCCGCCACAATCGCGACGGCCGCGGGCTGGCAGATGGGCGAGGGGAGGGACGAt‑cagcaagagccaaagaa > 1:360604/1‑227 (MQ=255) aTAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGCCTGAACGATGGTCGAAGCGAAGGGCGATGCGGCAGGGGCAAATcc > 2:578389/1‑239 (MQ=255) aTACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGGCTGGAATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGCCGTCCGGGGTCTGAACGATGGTCGACGCGAGAGGCGATGCAGCAGATGCAAAACCAGAt‑tttt > 2:507827/1‑239 (MQ=255) cTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGGGATGCAGCAGGGGCAAATCCAGAT‑tgttgggttg > 2:347088/1‑239 (MQ=255) cTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGATCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGATCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGGTAAGCCCGCCACAATCGCGTCGTCCGGGGCAGGAACGAGAGTGGCAGAGAGAGGAGAGGCGGCAGGGGCAAAACCAGTA‑tggtgtgatg > 1:822086/1‑239 (MQ=255) tCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑Tgttgggttgt > 2:636812/1‑239 (MQ=255) tCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑Tgttgggttgt > 2:663356/1‑239 (MQ=255) cgggcgcCAGACGGCGAAAAATGGCGCGGATATGTTCATGCACGCAAAAATTTC‑CCTGCACAAAAAGGACCACGGGCGGTGGGCCA‑TCGCTTTGCTTTGGTCGGGCATGGTAAGCAGGCATG‑TTACCCCCTTGTGAAGGGATAGGGGGGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑tgttgtgttg < 2:96851/234‑1 (MQ=255) cgcCAGAAGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAACTTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCAGG‑TTATCCCCTTTAGAAGGGATCGAGGTGAAGCCCGCCCAAATCGCTTCATCCGGGGTCTGAACGATGGTCGAAGCGAAAGGCTATGCAGCAGGTGGCAATACCGAT‑TGTTTGTTTGTTGcaa > 1:191587/1‑237 (MQ=255) ccAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAAc > 2:369842/1‑178 (MQ=255) ccAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGGGTTGTTGCCATg > 2:79578/1‑239 (MQ=255) gACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCCGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTa > 2:776250/1‑239 (MQ=255) gACGGCAAAAAATGTCGCGGTTATTTTCATGCACGCAAAAATTTC‑CCTGCACTCCAATGCCCTCGGGCAGTGGGCCA‑TCGCTTTGTTTTGGTCTGGCATGGAAAGCAGGCATT‑TTATCCCTTTGTGAAGGGAAAGAGGTAAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTa < 2:822106/239‑1 (MQ=255) cGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGTGGGTCGAAGCGAGAGGGGATGCAGCAGGGGGCAATCCACGT‑TGTTGGGTGGTTGCCATGGTactt > 1:96851/1‑234 (MQ=255) cGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTTTTGCCATGGAATtct > 2:629280/1‑238 (MQ=255) aaaaTGGCGCGGATATGTTCATGCCCGCAAAAAATTT‑CCTGCACTAAAATGACCCGGGGCAGTGGGCCA‑TCTGTTTGCTTGGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCTTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCg < 2:1158541/237‑1 (MQ=255) tGTTCATGCCCCCCAAAATTTC‑CCTGCACTACAATGCCCGCGGGCGGGGGCCCA‑TCGTTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGGGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATg < 2:996095/239‑1 (MQ=255) ttCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGGGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTTTAAAAATAtt > 2:993915/1‑239 (MQ=255) cATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGGGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTTAACGATGGTCCAAGCGAGAGGCGATGCAGCAGGGGCAAATCCGGAT‑TTTTTGTTTGTTGCCATGGAATTTTCCGTAACCTTTAAAAAATGtaag > 1:751052/1‑236 (MQ=255) gCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGTGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGGCTGAACGATGGTCGAAGCGTGAGGCCATGAAGCAGGTGCAAATCCAGAT‑GGTTGTGTTGTTGCCAGGGGATTTTCCGTCCCCTTATAAAAATGTTgcgga > 1:1288337/1‑237 (MQ=255) gCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCTGAGTCCGGGGTCTGAACGATGGGCGAAGCGAGCGGCGATGCAGCAGGGGCAAAGCCAGAT‑TGTTGTGTTGTTGCCATGGAGTTTCCCGTACCCTTATAAAAAtaatcgaca > 1:303352/1‑231 (MQ=255) ccGCAAAAATTTT‑CCTGCCTTCAAATGACCCTGGGAAGGGGCCCA‑TCCTTTTTCTTGGGTTGGGAATGGTAAGCGGGCTGT‑TTACCCCTTTGGGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAt < 2:497534/238‑1 (MQ=255) cGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGAATTCTCCGTACCCTTTTAAAAATGTTGCGCAATg > 2:827922/1‑239 (MQ=255) cgaaaaaaTTTCCCTGCCCTAAAGTGACC‑CTGGGCGGGGGCCATTGGTTTTGTTTGGGTCGGGAAGGGAAAGGAGGCATT‑TTCCCCCTTTGTGAAGGGAAAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAt < 2:993432/235‑1 (MQ=255) aaTGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGACATGGTATTATCCGTACCCTTATAAAAATGTTGCGCATTGTTAACTATAGTCAGC‑ATGcaac > 2:764082/1‑239 (MQ=255) tgccccctggttgggggtcttCGTTTTGGTTGGGTCGGTGAGGGTAAGCAGGGTTG‑TCCCCCTCTGGGGAAGGGATAGGGGTGCAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGcaacaa < 1:1278962/219‑1 (MQ=255) aCCACTGGCAGTGGGCCA‑TGGATTTACTTTGGACTGGCATGGTAAGCGGGCATG‑TTATCCCCTTGTGCAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGCTATGAACGATGGGCGGAGCGCGGGGAGATGCCGCGCGCGCAACTGCAGATGTGTTG‑GGTGTGACAATGGTATTGTCTGTACCATTATAAAAAGTTTGCGAAATGTTGACTATTGTCACT‑ATGcaaaaat > 1:893974/1‑237 (MQ=255) aCCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAACAATGTTTGGCAATGTTAACTATAGTCAGC‑CTGCAACaaa > 2:23704/1‑238 (MQ=255) tGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCGGC‑ATGCAACAAATAACAt > 2:557183/1‑239 (MQ=255) tGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCCCAATGTTAACTATAGTCAGC‑ATGCAACAAATCCCAt > 2:362818/1‑239 (MQ=255) tGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGGGCAAATCCAGAT‑TGTTGAGTTGTTGCCATGGGATTTTCCGAACCCTTTTAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACAt > 2:1229937/1‑239 (MQ=255) tGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGCCTGAACGATGGTCGAGGCGAGAGGCGATGACGCAGTTGCAAATCCAGAT‑GGTTTGGTTGTTGCAATGGTATTCTCGGTGCCCTTATAAAAATGTCTCGATAGGTTAACTTTCGCCAAC‑TTGcaacaacacaaat > 1:419408/1‑232 (MQ=255) tGGCAGTGGCCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGTTAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCCAAGTTACCTATAGTCCGA‑TTGCAACAAATCACAt > 2:705566/1‑239 (MQ=255) ggCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAc > 2:651266/1‑205 (MQ=255) gtttttgtttgtggtgtcATGGTAAGCGGGAATG‑TTACCCCTTTGGAAAGGGAAAGGGGTAAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTc < 2:419408/222‑1 (MQ=255) ggtttgctttgGT‑TGGGAAGGGAAAGAGGCATGTTTATCCCCTTGGAAAGGGATGGGGGGAAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTc < 2:1053991/237‑1 (MQ=255) gctgtgtttgggttgggaaTGGCAAGCAGCCATT‑TTACCCCGTGGGGAAGGGATAGAGGGAAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTc < 2:854045/221‑1 (MQ=255) tgctttgGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCt > 2:303608/1‑239 (MQ=255) ggtggggagttggaaGCAGGATTG‑TTTCCCCCTTGTGAAGGGAAGGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg < 2:1288337/226‑1 (MQ=255) gggcgggctgggtaaggggcgaTG‑TTACCCCATTGTGAAGGAAAAGGGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg < 2:457724/218‑1 (MQ=255) gTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGGCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑GGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATTTTAATCATAGTCAGA‑ATGCAACAAATAAACATGCCTGAATCGTCCCATCtttttgcc > 1:393432/1‑235 (MQ=255) ctgcgaTGGTAAGGCGGCAGT‑TTACCCCCTGGTGAAGGTATGGAGGTGAAGCCCGCCATAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAg < 2:515700/234‑1 (MQ=255) tgggagtggaagcggggatttttACCCCTTTGGAAAGGGATAGGGGGGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAg < 2:393432/218‑1 (MQ=255) tGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAg > 2:257957/1‑238 (MQ=255) gCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCGGCAGGTGGAAATCCAGGT‑TGTTGGGTTGGTGCCGTGGTATTTTCCGTACCCTTATAATAATGTTTCGCAATGTTAACTTTAGTCTAC‑ATTCACCAAATCACATTGCCTGAATccgggttcttataaacaattg > 1:804761/1‑218 (MQ=255) gCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg > 2:579810/1‑233 (MQ=255) gCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATCAACCAAATCCCATTGCCTGAATCGGCTCATCTTTTATTCAGt > 2:558501/1‑237 (MQ=255) gaaggagaggggtgcattattcccccTTGGGAAGGGAGGGGGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTc < 2:1031075/216‑1 (MQ=255) gTAAGCAGTCATG‑TTATCCCCTTGTGAAGGGAGAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGGCTGAACGATGGCCGAAGCGAGAGGCGATGCAGCAGGCGCAAATCCAGCT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATCTTAACTA‑AGGTAGCAATGCAACAAATCACATTGCCCGAATCGGCTCATCCTTTATCCacacttctct > 1:58194/1‑230 (MQ=255) gTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAg > 2:984626/1‑239 (MQ=255) gttG‑TTAGCCCGTTGAGGAGGGAGGTAGGGGGAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAgg < 2:1158956/236‑1 (MQ=255) aTG‑TTATCCCTTTGTGAGGGGATAGGGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAggg < 2:233405/238‑1 (MQ=255) tG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCATTGTTAACTATAGTCAGC‑ATGCAACAAATAACATTGCCTGAATCGGCCCATCTTTTATGCCGTCCTCCAGAATGAAggggg > 1:233405/1‑237 (MQ=255) cccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCCGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACACATCACATTGCCTGAATCGGCTAATCTTTTATGCAGTCTTGCAGAATGAAGGGGGGTTTAtg > 1:883575/1‑239 (MQ=255) cccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTAtg > 2:1000134/1‑239 (MQ=255) tgtgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTATGTGAt > 2:1026128/1‑239 (MQ=255) tgtgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGGGATTTATGTGAt > 2:677030/1‑239 (MQ=255) gtgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATAACATTGCCTGAATCGGCTAATCTTTTATGCAGTCCTGCAGAATGAAGGGGGATTTATGTGAtt > 2:833637/1‑239 (MQ=255) aTAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCTGGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTCGGCAATGTTAACTATAGTACGC‑ATGCAACAAATCCAATTGCCGGAATCGGCTAACATTTTATGAAGACCTGCAAAATCAAGGGGGATTTTTGTGATTGGCATc > 1:783883/1‑237 (MQ=255) aTAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTATGTGATTTGCATc > 2:62395/1‑237 (MQ=255) aTAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGGGATTTATGTGATTTGCATc > 2:891092/1‑237 (MQ=255) agagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCCGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTATGTGATTTGCATCACtt > 2:1220811/1‑239 (MQ=255) gagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATCCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCGGAATGAAGGGTGATTTATGTGTTTTGCTTAACttt > 1:274690/1‑239 (MQ=255) | CTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTT‑CCTGCACTACAATGACCACTGGCAGTGGGCCA‑TCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATG‑TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT‑TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC‑ATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTATGTGATTTGCATCACTTT > NC_000913/4015843‑4016306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |