BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,096,768	T→C	10.6%	E227G (GAG→GGG)	rhaB ←	rhamnulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,096,768	0	T	C	10.6%	266.5 / 2.2	108	E227G (GAG→GGG)	rhaB	rhamnulokinase
Reads supporting (aligned to +/- strand): ref base T (33/58); new base C (8/5); total (42/66)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CTCGCTGAAGCAGCCATAAGCCCATAATATTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTT  >  NC_000913/4096532‑4097002

cTCGCTGAAGCAGCCATAAGCCCATAATATTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAAactcc                                                                                                                                                                                                                                                 >  2:572661/1‑238 (MQ=255)

  cGCTGAAGCAGCCATAAGCCCATAATATTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑AGCGATAACCGCGCTGGCGGGATCATGGCTGGCAACGGCGACCACTGGGATCTCAtt                                                                                                                                                                                                                                               >  2:594998/1‑239 (MQ=255)

        agcagcCATAGGCCCATAATATTTTTCAGCACCCGATAGCGTCCTTCCGCCCCGCCTTCATTGGCGATGTTGGCTGCCAGTGCCGTTTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTg                                                                                                                                                                                                                                         <  2:94180/239‑1 (MQ=255)

        agcagcCATAAGCCCATAATATTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGGTAACCGCGCTGGCGGTATCATGGTAGGCAACGGGGCACACTGGAGTTCCATTAaccc                                                                                                                                                                                                                                          >  2:58450/1‑237 (MQ=255)

        agcagcCATAAGCCCATAATATTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAAACCCCCTGGCGGAATCATGGCTGGGAACTGCGACCCCTGGAATCCCATTaacacc                                                                                                                                                                                                                                         >  2:950439/1‑234 (MQ=255)

             ccATAAGCCCATAATATTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGCCGGCATAATGGCTGGCCACGGCGACCCCCGGGATCTCATTACCCTCCCGGc                                                                                                                                                                                                                                    >  2:1227117/1‑239 (MQ=255)

               ttcggCCCTTATTATTTTTCGGCGCCCGGTGGCGGCCGTCCGCCCCGCGTTTATTGGGGATGTGGGGTGCCAGTGGCGTGTCATTGGTAAGGGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTGAACGGCGA‑GGCGATAATCGCGCTGGCGGTATCATGGCTGCCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCaa                                                                                                                                                                                                                                  <  1:1237661/235‑1 (MQ=255)

               ctAAGCCCATGATTTTTTTCCGTCCCCCATAAGGCTCTTCACCCCCGCCTCAATTGGTGTTTTTGGCTGCCCGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGCGCC‑AGAAGGGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACTGCGA‑GGCGATAACCGCTCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCaa                                                                                                                                                                                                                                  <  1:1193281/238‑1 (MQ=255)

                     gtattatttttttCAGCCCCCGTGAGCGCTCTTCCGCCCCGCCTTCATGGGTGATGTTGGCTGCCAGTTCCGTGTCATTGGTAAATTGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAg                                                                                                                                                                                                                            <  1:1225868/231‑1 (MQ=255)

                     ccctattatTTTTCCGCCCCCGTTTGCGACCTTTCCCCCCGTCTTCCTTGGTGATGTGGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAg                                                                                                                                                                                                                            <  1:771761/233‑1 (MQ=255)

                     ccATTATTTTTTTCTGGCCCCGTTAGCGCCCTTCCGCCCCGCCTGCATGGGGGATGTTGGATGCCAGTGCCGGGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATAAATGGCCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAg                                                                                                                                                                                                                            <  1:462821/239‑1 (MQ=255)

                     ccATTATATGTTTCAACCCCCGATGGCGACCTTCCGGCCCGTCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGGCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAg                                                                                                                                                                                                                            <  2:510162/239‑1 (MQ=255)

                     ccATAATATTTTGCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAg                                                                                                                                                                                                                            <  2:185681/239‑1 (MQ=255)

                     ccAAAATATTTTCCAGCCCCCGAAAACGACCTTCCGCCCCGCCCTCATTGGTGATTTTGGCTGCCAGTGCCGTGTAATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAg                                                                                                                                                                                                                            <  2:1175875/239‑1 (MQ=255)

                      ctcatcttttttCATCACCCGGTGCCGTCCTTCCGCCCCGCCTTCATTTGTGATGGTGTCTGCCAGTGCCGTGTAATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTCCCCTGCGGGCAAATCCAGt                                                                                                                                                                                                                           <  1:594998/232‑1 (MQ=255)

                      caaaataTTTTTCAGCCCCCGTTAGCGGCCTTCCGCCCCGCCTTCAATGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGt                                                                                                                                                                                                                           <  2:177126/236‑1 (MQ=255)

                      cATAATTTTTTTCAGCCCCCGATAGCGGCCTTCCGCCCCGCCTTCATTGGTGATGTTGGAGGCCAGTGCCGTGTCAGTGGTAAATGGCGTCTGGCGTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGt                                                                                                                                                                                                                           <  2:587946/239‑1 (MQ=255)

                      cATAATATTTTACAGCCCCAGATACCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGGGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGt                                                                                                                                                                                                                           <  2:157122/239‑1 (MQ=255)

                      aataataTTTTTCAGGCCCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCAGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGt                                                                                                                                                                                                                           <  2:1143757/238‑1 (MQ=255)

                      aataataTTTTTCAGCACCCGATCGCGCCCTTCCGCCCCGCCTTCATTGGTGATGTTGGGTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCGGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGt                                                                                                                                                                                                                           <  2:1272591/238‑1 (MQ=255)

                       ataataTTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGGGACCACTGGAATTGCATTACCCTGCGGGCAAATCCAGt                                                                                                                                                                                                                           >  2:767348/1‑238 (MQ=255)

                       ataataTTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCCCCTTACCCTCCGGGGAAATCCAGTg                                                                                                                                                                                                                          >  2:565140/1‑239 (MQ=255)

                       ataataTTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATACTGGCTGGCAACGGCGGCCACTGGAATCTCATTCACCTTCGGGCAAATCCAGTg                                                                                                                                                                                                                          >  2:1237661/1‑239 (MQ=255)

                       ataataTTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGGATCATGGCTGGCAACGGCGACCACTGGGATTTCATTACCCTGCCGGCAAAACCAgg                                                                                                                                                                                                                           >  2:987303/1‑237 (MQ=255)

                       ataataTTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGGAACATGGCAGGAAACAGAGACCCCTGGAAACCCCTTACCCTGCGGGGAAATCCAGt                                                                                                                                                                                                                           >  2:1193281/1‑238 (MQ=255)

                       ataataTTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGAATCATGGCTGGCAACGGCGACCCCTGGAATCCCATTACCCCTCGGGCCAATCCAGTg                                                                                                                                                                                                                          >  2:203173/1‑239 (MQ=255)

                           tatTTTTCAGCACCCGATAGCGCCCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGGGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCa                                                                                                                                                                                                                             <  2:1291982/232‑1 (MQ=255)

                            atTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTGCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTa                                                                                                                                                                                                                     <  2:743638/239‑1 (MQ=255)

                                 tCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCCTGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTCTGa                                                                                                                                                                                                                  >  2:1225868/1‑237 (MQ=255)

                                 tCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACa                                                                                                                                                                                                                <  2:383097/239‑1 (MQ=255)

                                     cACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACCGGAATCTCCTTACCCTGCGGGCAAATCCAGGGGCCCATGACATTa                                                                                                                                                                                                             >  2:924219/1‑238 (MQ=255)

                                     cACCCGATAGCGACCTTCCGCCCCGCCTTAATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTGAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑GGTTTAACGGCGA‑GGCGATAACCGCGCCGGCGGAATCATGCCTGGCAACGGCGGCCCCCGGAATGCCCTTACCCGTCGGGCaaaaaccgaggccccagcctggc                                                                                                                                                                                                             >  1:1291982/1‑218 (MQ=255)

                                          ggaaGCGACCTTCCGCCCCGGCTTCCTTGGTGATGTTGGCTGCCAGGGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGa                                                                                                                                                                                                      <  2:169989/236‑1 (MQ=255)

                                                    ttCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGGATAATGGCTGGCAACGGCGACCACTGGGAATTCATTACCCTGCGGGCAAACTCAGTGACCTTTGACATTA‑CCCGGATGCgcctggg                                                                                                                                                                                            >  2:663334/1‑233 (MQ=255)

                                                      ccgccccgcgTTCTTTGGTGAGGTTGGCTGCCAGTGCCGTATCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGa                                                                                                                                                                                         <  2:317968/239‑1 (MQ=255)

                                                      cccgcccggcTTCCTGGGTTGTGTTGCCTTCCAGGGCCGTGTCATTGTAAAATGGCGTCTGGTTTGCGAAGCCCGTCAATGTCCGTGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCTA‑GGCGATAACCGCGCTTGCGGTATCATGGCTGGCAACGGCGAACACTGGAATCTCAGTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGa                                                                                                                                                                                         <  1:782053/235‑1 (MQ=255)

                                                            cgccTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGAACACTGGAATCTCATTACCCTTCGGGCAAATCCAGTGACCTATGACCTTA‑CCCGGATGCGTC‑GGGCCACCC‑A‑Ac                                                                                                                                                                                 >  2:114506/1‑239 (MQ=255)

                                                            acacttgtttgggtgtGTCGGCTGCTCGTGCGGTGTGATTGGTAAAGGGCGTCTGTGTGTCGAAGCCCATGAATAGCCAGGTGCC‑AGAAGAGGGAT‑AGGCAGCAACGGGGC‑CGTTTAGCGGCGA‑GGCGATAACCTCGCTGGCGGTATCATGGCTGTCAACGTGGACCACTGGAATCTCATTACCCTGCGGGCAAATCGAGTGACCTATGACAGTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑Ac                                                                                                                                                                                 <  1:854545/224‑1 (MQ=255)

                                                             ggcTTCATTGGGGATGGTGGCCGCCAGGGCCGTGTCTTTGGTAAATGGCGTCTGGCTTTCGAAGCCCAACAATGACCAGGTGCC‑AGGAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑Acc                                                                                                                                                                                <  2:726775/237‑1 (MQ=255)

                                                             gccTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑Acc                                                                                                                                                                                <  2:951369/239‑1 (MQ=255)

                                                             gccTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑Acc                                                                                                                                                                                <  2:497252/239‑1 (MQ=255)

                                                                 tCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGc                                                                                                                                                                            <  2:746565/239‑1 (MQ=255)

                                                                      ggTTATGTTGGCTGCCAGGGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTttgt                                                                                                                                                                       <  2:797585/239‑1 (MQ=255)

                                                                      ggTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGGCCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTttgt                                                                                                                                                                       <  2:918126/239‑1 (MQ=255)

                                                                          aTGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGGGCC‑AGAAGAGAGAT‑AAGCAGCAAGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGGATCTTGGCTGCGAAAGGCGGAACCTGGAAACTCATTACCCCGCGGGGAAATCCCGGGACCTATGACATTA‑ACCCGGTGCGTCGGGGGGACAA‑A‑ACCGGACtttttgg                                                                                                                                                                     >  1:810738/1‑234 (MQ=255)

                                                                            gTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCCATTACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTTTTGGcc                                                                                                                                                                  >  2:1207863/1‑238 (MQ=255)

                                                                              tGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGGGGCCTATGACATTA‑CCCGGATGGTTC‑GGGCGACCA‑A‑ACCAGGCGTTGTTGGcccc                                                                                                                                                                >  2:1019762/1‑238 (MQ=255)

                                                                                 cTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCt                                                                                                                                                             >  2:764743/1‑238 (MQ=255)

                                                                                  tGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCCACGGCGACCACCGGAATCTCATTACCCTGCGGGCAAACCCACGGACCTATg                                                                                                                                                                                                                   >  2:609550/1‑187 (MQ=255)

                                                                                  tGCCAGTGCCGTGTCATTGGTAAAGGGCGGCTGGCTTTCGAAGCCCATCAATGACCGGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTGTGTTGGCCCCGCTc                                                                                                                                                            <  2:330181/238‑1 (MQ=255)

                                                                                       gttccgttgttttGGTAAATGGCGGTGGGTTTTCGAAGCCCCTCAATGCCCGGGTTCA‑AAAAGAGAAAT‑AAGCTGCACGGGGGC‑CGTTTAACGGCGG‑GGGTATAACCGCGCGGGCGGTAGCATGGCTGGCAACGGCGACCACGGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGAGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGcc                                                                                                                                                      <  1:663334/228‑1 (MQ=255)

                                                                                          ccGGGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTc                                                                                                                                                            <  2:753093/230‑1 (MQ=255)

                                                                                              gtCATTGGGAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAAACGCGCTGGCGGTATCCTTGCTGGCAATCGGGACCACTGGAAGCCCATTACCCCTGGGGGAAATCCCGTGGCCCCATACATTC‑ACCTGATGGCTT‑GGGCGGCCA‑ACACACGGCTGT‑TTGGCCCCGCCCCAcccccggagg                                                                                                                                                >  1:982528/1‑232 (MQ=255)

                                                                                                  ttGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTTAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTAACAATGCCGGCAACGGGCAGCACTGGAAACTCATTACCCTCCGGGCAAATCCCGTCACACATGACATTA‑CCCGGAGGCGCC‑GGGCGCCCC‑A‑ACCAGCCGTTCTTTCCCCCCCCCCCCGCCAgaaccgcc                                                                                                                                             >  1:1263445/1‑230 (MQ=255)

                                                                                                    ggTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCCAGGCTGGCAAACGCGCCCACTGGAATCTCATTTCCCTGCGGGCAAATCCAGAGACCCTTGAACTTACCCCGGAGGC‑CC‑GGGCGACCA‑A‑AACACGCCTTTTGGGcccccgccacccccccagccgggccct                                                                                                                                         >  1:753093/1‑216 (MQ=255)

                                                                                                        aaTGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCAAGCCCGGAGCGACTCGTccc                                                                                                                                      >  2:948186/1‑238 (MQ=255)

                                                                                                              gctgggCTTTGTAAGCCCACAAATGACCAGGTGCC‑AGAAAGGGGTT‑AAGCCGCACGGTGGC‑CTTTTAACGGGGA‑GGCGATAACCGCCGTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGTCATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑CCCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAgtcgt                                                                                                                                <  1:1100467/234‑1 (MQ=255)

                                                                                                              ctttgtcTTTCGGAGCCCAACAAGGCCCCGGGCCC‑AGAAGAGAGAT‑AAGCAGCACGTGATC‑CGTTTAACGGCGG‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAgtcgt                                                                                                                                <  1:948186/232‑1 (MQ=255)

                                                                                                               tttGGCTTCCGAACTCCATCAATGACCCGGTGCC‑AGAAGGGAGAT‑AAGTAGCAGTGGTGC‑CGTTTAACGGCGG‑GGCGAGAACCGGGCTGGCGGTATGATGGTTGGCAACGGCGATCCCTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGGC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCTGCTCCACGCCAGTAGCGACTCGTCCCAgtcgtc                                                                                                                               <  1:203173/236‑1 (MQ=255)

                                                                                                               tCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGAGGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGGGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACCCGGCCCAgtcgtc                                                                                                                               >  2:822561/1‑239 (MQ=255)

                                                                                                               tCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGGTGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGCACCGTcccccacgtc                                                                                                                               >  2:1100467/1‑231 (MQ=255)

                                                                                                                cTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATTCGTC‑GGGGGACCA‑A‑AACAGGCTTTGTTGGCCCCGCTCCACGCCCGTAGCGGCTCGTCCCAgtcgtc                                                                                                                               >  2:556263/1‑237 (MQ=255)

                                                                                                                        ccgaGCCTACCAAGGACCAGGTGCC‑AGAA‑AGCGCTGAAGCGGCACG‑GTGCACCTTTAGGGGGGT‑GGAGATAACCGGGCTGGCGGTATCAGGGCGGGCAACGGCGGCCCAGGGAATCGCATTACCCTGGGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCtattgata                                                                                                                     <  1:751027/236‑1 (MQ=255)

                                                                                                                        cGAAGCCCATCAATGGCCAGGTGCCAAGAGGAGA‑AA‑AAGGCGCACGTGAGC‑CGTTTACCGGCGA‑GGGGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCtattgata                                                                                                                     <  2:331786/239‑1 (MQ=255)

                                                                                                                              ccATTAATGGCCAGGTGCC‑AGAAGAGGAAT‑AAGCAGCAGGTGAGC‑CGGTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTCCCCTGCGGGCAAAGCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGAc                                                                                                                <  2:1066116/238‑1 (MQ=255)

                                                                                                                                          ggggccc‑AGAAGAGAGAT‑AAGGCGCAGGGGGCG‑CGTTTAACGGGCA‑GGGGATACCCCGGCTGGCGGTAACATGGCGGGCAACGGGTGCCACTGGATTCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTG‑CCCGGATGCGTG‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTGCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTc                                                                                                  <  1:987303/234‑1 (MQ=255)

                                                                                                                                          aGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCCCTCCCCGCCAGGAGGGACTCGTCCCAGGCGTCCCTATTGATATTGACCAGTTGC‑GTGGTc                                                                                                  >  2:326251/1‑239 (MQ=255)

                                                                                                                                             tGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCg                                                                                                 <  2:743733/237‑1 (MQ=255)

                                                                                                                                               cc‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCCCGCCAGTAGCCACTCGTCCCAGTCGTCCGTATTGATTTTGACCAGTTTC‑GGGGTCGTGGc                                                                                             >  2:924430/1‑239 (MQ=255)

                                                                                                                                                  agaagaGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGc                                                                                                                                                       >  2:353015/1‑180 (MQ=255)

                                                                                                                                                     atggagaT‑AAGCAGCGCGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGGGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGtt                                                                                          <  2:181352/234‑1 (MQ=255)

                                                                                                                                                        gaaaT‑AAGCAGCACGGGGGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGt                                                                                       <  2:165616/234‑1 (MQ=255)

                                                                                                                                                        aggaT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGtat                                                                                     <  2:152364/237‑1 (MQ=255)

                                                                                                                                                           aT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTAACCCTCGGGCCAATCCAGGGACCTCTGACATTA‑CCCGGGTGCGGT‑GGGCGACCA‑A‑ACCAGGCTGTGTTGGCCCCGCGCCACGCCAGGAGCCACCCGTCCCCCGCGTCCCGATTGATTTTTGCCAGATTC‑GTgggggggggggtgggaaaa                                                                                   >  1:766382/1‑221 (MQ=255)

                                                                                                                                                               agcagcACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCAAGGCTGGCAACGGCGACCACTGGAATCTCATTACCCCGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGCC‑GGGCGACCA‑A‑ACCAGGCTTTGTGTGCCCCGCTCCCCGCCACGACCGACTCGGCCCGTGCGGCCCTATTGTTCTTTTCCAGTTTC‑GTGGGCGTgggggggggcatatcc                                                                                >  1:411903/1‑224 (MQ=255)

                                                                                                                                                                    caCGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCCCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGGCGGGGCGTTgggtaatttcaagtta                                                                          >  2:67155/1‑225 (MQ=255)

                                                                                                                                                                      cGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAAAGGCGACCACTGGAATCTCATTACCCCGCGGGCAAATCCAGTGCCCCATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCCCCCCACGCCAGTAGCGGCTCGCTCCAGCCCTCGTCATTGGCATTGACCCGTTGC‑GTGTTCGGGGCGTT‑GTATATATCCCA‑Gtt                                                                         >  1:152364/1‑236 (MQ=255)

                                                                                                                                                                              cGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTG‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCtat                                                                                                                          <  2:465400/183‑1 (MQ=255)

                                                                                                                                                                               gTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCCTGGCTGGCAACGGCGAACACTGGAATCTCCTTACCCTGCGGGCAAATCCCGTGACCTATGACATTA‑CCCGGATTCGCC‑TGGCGGCCACA‑ACCTGGCTTT‑TGGGCCCCGCTCCACCCCCGGTGCGCCTCGTCCCAACCGTCCCTATTTGAATTTACCCGTTGCTGTGGCCG‑GGCGGTGGTATAT‑TCCCAGGTTCAttggcgcg                                                               >  1:651117/1‑232 (MQ=255)

                                                                                                                                                                                 ttAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGt                                                             <  2:372169/238‑1 (MQ=255)

                                                                                                                                                                                     cGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAACCCAGTGACCTATGACATTA‑GCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGc                                                        <  2:966165/239‑1 (MQ=255)

                                                                                                                                                                                          a‑ggcgaTAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCGCGTCGTCGCTTTTGATATTGACCAGTTGC‑GGGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGGTaa                                                   >  2:112092/1‑239 (MQ=255)

                                                                                                                                                                                            ggcgaTAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATaa                                                   <  2:411903/238‑1 (MQ=255)

                                                                                                                                                                                            ggcgaGAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCTCTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATaa                                                   <  2:142589/238‑1 (MQ=255)

                                                                                                                                                                                                     gcgcctggggggtgtaTGGCTGGCAACGGCGACCACTGGAATCTCATTACCCGGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAAt                                         <  2:378289/224‑1 (MQ=255)

                                                                                                                                                                                                     cgcgCTGGCGGTATCGTGGCTGGCAACGGCGACCACTGGAATCGCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTT‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAAt                                         <  2:1064546/239‑1 (MQ=255)

                                                                                                                                                                                                     cgcgCTGGCGGTATCATGGCTGGCACCGGCGACCACTTGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAAt                                         <  2:857723/239‑1 (MQ=255)

                                                                                                                                                                                                       cgcTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGGCGCTATTGGTATTGACCAGTTGC‑GTGGTCGGGGCGTTGGAATAT‑TCCCA‑ATTCAATTTGCCGGTCAGGCGATAACtcagagaaaca                                       >  1:254457/1‑229 (MQ=255)

                                                                                                                                                                                                           ggCGGTATCATGGGCGGCAACGGCGACCACTGGAATCTCATTACCCGGCGGGCAAATCCAGTGACCTATGACATTC‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGc                                    <  2:1263445/238‑1 (MQ=255)

                                                                                                                                                                                                            gCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCTGCGCTATTGATATTGGCCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTTCCGGTCAGGCGATAACTGAAGTAAACCGGCa                                   >  2:1050389/1‑238 (MQ=255)

                                                                                                                                                                                                                 aacATGGCTGGCAACGGCGACCCCTGGAATCTCATTCCCCTGCGGGCAAATCCAGTGACCTATGACATTT‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGTTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATcagc                              <  2:319339/236‑1 (MQ=255)

                                                                                                                                                                                                                 aTCATGGCTGGCAACGGCGACCCCTGGAATCCCATGACCCTGCGGGCAAATCCAGTGACCTATGACATTC‑CCCGGATGCGTC‑GGGCTGCCA‑A‑GCCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATcagc                              <  2:160133/238‑1 (MQ=255)

                                                                                                                                                                                                                 aTCATGGCTGGCAACGGCGACCACGGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATcagc                              <  2:348962/238‑1 (MQ=255)

                                                                                                                                                                                                                  tCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATccccag                            >  2:538740/1‑234 (MQ=255)

                                                                                                                                                                                                                          ggcAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGCTAACTGAAGTAATCCGGCATCAGCagaggtgggg                    >  2:1067162/1‑233 (MQ=255)

                                                                                                                                                                                                                          ggcAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACCCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGCTATTGTCCAGGTGC‑GTGGTCGTGGCGTTGGGTTAT‑TCCCA‑GTTCAACTTGCCGGCCAGTCGCTAAATTAGGGAATCCGGCactaccaaatcgggg                     >  1:1112256/1‑224 (MQ=255)

                                                                                                                                                                                                                               cGGCGACCACTGGAATCTCATTGCCCTGCGGGCAAATCCAGTGCCCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑TCCAGGCTTTGTTGGCCCCGCTCCACGGCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAtg               <  2:180287/239‑1 (MQ=255)

                                                                                                                                                                                                                                  gcaCCTCTGGAATCCCTTTACCCGGCGGGCAAATCCCGGGACCTATGCCATTC‑CCCGGAGGCGGC‑GGGCGCCCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAtgtg             <  1:767348/236‑1 (MQ=255)

                                                                                                                                                                                                                                   gACCACGGGAATCCCATTCCCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAtgtgt            <  2:509079/238‑1 (MQ=255)

                                                                                                                                                                                                                                             aTCCCATTACCCTGGGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGTAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGtt  <  2:110533/238‑1 (MQ=255)

CTCGCTGAAGCAGCCATAAGCCCATAATATTTTTCAGCACCCGATAGCGACCTTCCGCCCCGCCTTCATTGGTGATGTTGGCTGCCAGTGCCGTGTCATTGGTAAATGGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCC‑AGAAGAGAGAT‑AAGCAGCACGTGAGC‑CGTTTAACGGCGA‑GGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATGACATTA‑CCCGGATGCGTC‑GGGCGACCA‑A‑ACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGC‑GTGGTCGTGGCGTTGGTATAT‑TCCCA‑GTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTT  >  NC_000913/4096532‑4097002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`