BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,217,359	T→G	14.9%	G84G (GGT→GGG)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,359	0	T	G	14.9%	273.0 / 8.0	117	G84G (GGT→GGG)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand): ref base T (19/71); new base G (23/2); total (43/74)
Fisher's exact test for biased strand distribution p-value = 9.46e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

AACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGC  >  NC_000913/4217124‑4217596

aacaaATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCCAGCGCGT‑CAACAGGCGA‑AACCGGGGAtt                                                                                                                                                                                                                                              >  2:524089/1‑239 (MQ=255)

  caaATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGa                                                                                                                                                                                                                                            >  2:1066987/1‑239 (MQ=255)

    aagttaagaaTTACCAAAAGTGTGGACTCAACCGGGTGGTGAAGGCATTACTCGCCCATGCGGTGCGGAAGATGT‑GGTGAAATTGCGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAg                                                                                                                                                                                                                                          <  1:1085475/234‑1 (MQ=255)

    aaatGAAAAATTACAGAAAGGGTGGACTCAACCGGTTTGGGAAGGCATTACCCGACCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAg                                                                                                                                                                                                                                          <  1:379047/236‑1 (MQ=255)

     aTTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTT‑CAACAGGCGG‑AAGCGGGGAtttgaag                                                                                                                                                                                                                                         >  2:906648/1‑234 (MQ=255)

              ttACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGGGA‑AAGCGGGGATTGAAGGAGGCTa                                                                                                                                                                                                                                   >  2:1270445/1‑236 (MQ=255)

                        agTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGGATTGAAGCAGGCTATCTTTCGGGAAgg                                                                                                                                                                                                                      >  2:586249/1‑239 (MQ=255)

                            gACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGGCAGGCGCTC‑CAAAAGGCGA‑AAGCGGGGATTTGAGCAGACTTTCTTTCgggtaggccgg                                                                                                                                                                                                                  >  2:991843/1‑231 (MQ=255)

                                 aaCCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCg                                                                                                                                                                                                                            <  2:1212208/224‑1 (MQ=255)

                                   ccGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAgcggc                                                                                                                                                                                                           <  2:820380/239‑1 (MQ=255)

                                   ccGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAgcggc                                                                                                                                                                                                           <  2:388936/239‑1 (MQ=255)

                                    tgttttaaagggacgttACCCTCCCCTACAGGGGGGAAGATTG‑GGTGAAATTACCGGGTTCAGTCAATCCTGAATGGCCGCTGGGGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAgcggcg                                                                                                                                                                                                          <  1:1226395/224‑1 (MQ=255)

                                    cagttttttataagagaccgcGCCCATACAGGTGGGAAGATGG‑GGTGAAATGACGCGGTTCAGTCAATCCTGAATGCACGCTTGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAgcggcg                                                                                                                                                                                                          <  1:1154738/219‑1 (MQ=255)

                                                     aCTCGCCCATACAGTGCGGACGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATATGGCGTCTGCTGAACGGTGAGTCGAAAAAAGGCTACACCAACAGCCTAGGCGCAC‑TAACTGGCGGTCAAGCACGG‑CACCAGGAGA‑AAGGGGGGCTTGTAGAAGGCGATCTTTCGGGAGGGCAGGGAGcggggggcaccacaccggcgg                                                                                                                                                                                          >  1:1245181/1‑223 (MQ=255)

                                                     aCTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CCACAGGCGC‑AAGCGGGGATTTAAACAGTCTATCTGTCGGGGTGGCAGGTAGCGGCGGG‑CGCCAACCCggggg                                                                                                                                                                                         >  2:1250420/1‑238 (MQ=255)

                                                      cccGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGcc                                                                                                                                                                                       <  2:1227448/237‑1 (MQ=255)

                                                          cccATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CCACAGGCGA‑AAGCGGGGATTGAAGCAGGCTTTCTGTCGGGGTGGCAGGGACGGGCGGA‑CGCTAAccccctgttttttt                                                                                                                                                                                   >  2:486768/1‑227 (MQ=255)

                                                           ccctaccggggggAAGTTGG‑GGTGAAATTACCGGGTTCAGTAAATCCGGAATGCAAGCGGGGGCAACTGGGCCGAGGGAAAAGGGGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCAt                                                                                                                                                                                  <  1:791485/228‑1 (MQ=255)

                                                           ccatcccgggCGGAAG‑TGTGGGTGAAATGTCGCGGTTCAATCAATCCTGAATGCACGCTGGCCCAACGGGGCGGAGGGAAAATGTGGCCTCTGCTTCACGGTGAGGCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGGCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCAt                                                                                                                                                                                  <  1:152649/230‑1 (MQ=255)

                                                           ccATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CGACAGGCGA‑AAGCGGGTATTGAAGCAGTCTTTCTGTCGGGGAGGACGGGAGAGGCGGA‑CGGTCACCCggggggccacca                                                                                                                                                                                  >  2:991028/1‑232 (MQ=255)

                                                           ccATACAGGGCGGAAGGTGG‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCAt                                                                                                                                                                                  <  2:144474/239‑1 (MQ=255)

                                                              tACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGGATTGAAGCAGCCTATCTGTCGGGATGGCAGGGAGCGGCGGA‑GGCTAAACCGGCGGGCACCATgga                                                                                                                                                                               >  2:225231/1‑237 (MQ=255)

                                                              tACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCCG‑CAACAGGCGA‑AAGCGGGGATTGAAGCCGCCTATCTGTCGGGATGGCAGGGAGCGGCGGA‑CGCTAACCCGGCGGCCAGCATGTa                                                                                                                                                                               >  2:562666/1‑239 (MQ=255)

                                                                   tGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCCGGGAGAGGCGGA‑CGCTAACCTGGCGGCCAGCATGTTTCCgg                                                                                                                                                                          >  2:178294/1‑239 (MQ=255)

                                                                   tGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCAATTATCCgg                                                                                                                                                                          >  2:238170/1‑239 (MQ=255)

                                                                   tGCGGAAAATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGACAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGGAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGGATACgg                                                                                                                                                                          >  2:764544/1‑239 (MQ=255)

                                                                   ggCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGACAGCACGCTGGCGCAACTGGGCGTAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTggcggc                                                                                                                                                                                        <  2:665064/224‑1 (MQ=255)

                                                                     cGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CCACAGGCGA‑AAGCGGGGATTGGAGCCGTCCATCTGTCGGGGTGGGAGGTAGCGGCGGA‑GGCCAAACTGGCGGCCCGCAGGTATcccgg                                                                                                                                                                         >  2:544108/1‑235 (MQ=255)

                                                                     cGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACCGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGAGGGCCGGGAGCGGCGGA‑CGCCAAACTGGCGGCCAGCATGTATCCgggt                                                                                                                                                                        >  2:241511/1‑237 (MQ=255)

                                                                       gAAGATGT‑GGTGAAATTACGGGGTTCAGTCAATCCTGAATGCCCGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATc                                                                                                                                                                       <  2:593290/238‑1 (MQ=255)

                                                                        aaGATGT‑GGTGAAATTCCGCGGTCCAGTCAATCCTGAATGCACGCTGGCGCAACGGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCa                                                                                                                                                                      <  2:48172/238‑1 (MQ=255)

                                                                        aaGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTg                                                                                                                                                                                                                                             >  1:1053762/1‑168 (MQ=255)

                                                                        aaGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTg                                                                                                                                                                                                                                             <  2:1053762/168‑1 (MQ=255)

                                                                          agaatgggtaaaaaaaatcgGTTCAGTAAATCCTGAAGGGACGTTGGGCAAACGGGGCCGAGGAAAAATGGTGAGTCGTCTGCACGGGTAGTCGAAAAAAGGCTACATCAACAGCCGCGGCGCAC‑GGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGTAGTCTATCTGTCGGGATGGCAGGTCGCGGCGGA‑CGCTAACCTGGCGGCAAGCATGTATCCGGATCAg                                                                                                                                                                     <  1:881965/220‑1 (MQ=255)

                                                                             gt‑ggtgAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGGCAGGGCCCG‑CAACGGGCGACAAGCGGGCTTGGAACCAG‑CTATCTTTCGGGGTGGCAGGGAGGAGCGGAGCGTAAACCGGGGGCCCAGCgtgttccgcgcacaggtg                                                                                                                                                                  >  1:1183491/1‑224 (MQ=255)

                                                                             gt‑ggtgAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGCCGCAACTGGGAGCAGCGAAAATGTGGCGTCTGCTGCACGGGGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTGCGGCGCCG‑CAACAAGCGA‑AAGCGGGGATTAGAACAGCACATCTGGCTGGAGTGCCGGTCACGGCGGA‑CCCCGACCTAGTGGcccgacaagagccgggcaaggccg                                                                                                                                                                 >  1:598382/1‑216 (MQ=255)

                                                                             gt‑ggtgAAATTACGCGGTTCAGTCAATACTGAATGCACGCTGGCGCAACTGGGCGCAGCGGAAAGGTGGCGCCTGCTGCCCGGAGAGCCGCAAAACAGCCACTTCACACGCCTC‑GCGCCCATGCCTGGCGGTCGAGCTGTGCCAACAGCCCA‑ACG‑GGGTAGTCAAGCAGGTCATCTTTACGTACGGCAGATAGCGGCGGG‑AGGCAAACAGGCGGCCAACAAGttggcgtcaccttaaga                                                                                                                                                                >  1:665064/1‑223 (MQ=255)

                                                                                ggtgAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGGGCTG‑CAACAGGGGC‑AAACGGGGATGGAAGCGGAGTATCTGTCGGGGTGGCGGGTGCCGGGGGC‑GCCTAACCTCGGGGCCCGccgtaatcaggcgcagcggaca                                                                                                                                                               >  1:38164/1‑217 (MQ=255)

                                                                                        tACTCGTTTCATTCAGTCTTGAATGCCAGTTGGCGCAACTGGGCGCAGAGAAAAGTTGGCGTTGGCCGCCCGGTGGATCAGAAAAAGGGTAGGTCAAATGCCTCGCCGCAC‑TAACTGGCGGTCAGGCGCCG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGTCGGA‑CGCTAACCTGGCGGCTAGCATGTATCCGGATCAGTCTCTCTATCCGGCa                                                                                                                                                      <  1:241511/239‑1 (MQ=255)

                                                                                            aggTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACt                                                                                                                                                  <  2:1098138/238‑1 (MQ=255)

                                                                                               ttCAGTCAATCCTGAATGCACGCTGGCGCAACTTGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCa                                                                                                                                                                                                                                        <  2:691182/151‑1 (MQ=255)

                                                                                               ttCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGGATCCGGATCCGTCGCCCTATCCGGCAAACTCgg                                                                                                                                               >  2:1143850/1‑239 (MQ=255)

                                                                                               ttCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGGATTGAAGCAGTCTATCTGTCGGGGTGGCAGGGAGGGGCGGA‑AGCTAACCTGGCGGCCCGCATTGAACCGGATCAGGCCCTCTTTCCGGCCAACTCgg                                                                                                                                               >  2:1213359/1‑239 (MQ=255)

                                                                                                tCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCg                                                                                                                                                                                                                            <  1:958886/162‑1 (MQ=255)

                                                                                                tCAGGCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTc                                                                                                                                                 <  2:375731/236‑1 (MQ=255)

                                                                                                         ccTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGG‑CGCCAACCTGGCGGCCAGCATGTATCCGGGTCAGTCGCTCTATCCGGCCAACCCGGGG‑cccccctt                                                                                                                                    >  2:377082/1‑233 (MQ=255)

                                                                                                              aTGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGtggtgg                                                                                                                               <  2:618540/239‑1 (MQ=255)

                                                                                                              aTGCACGCGGTCGCACATGGGGGTAGCGAAGATGTGGCCTCTTCTGACCGGTGACTGAAAAAAAGGCTACTGCAAAGGCCACGGCACAC‑TGCATGGCCGTCAGGTGGTG‑GCACAGGCGA‑AAGCGGTCATTGAAGCAGTCCATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCAGAACAGTCGCTCTATCCGGAAAACACGGTG‑CCAGCTGtggtgg                                                                                                                               <  1:544108/239‑1 (MQ=255)

                                                                                                                  ccGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCTTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGc                                                                                                                            <  2:152064/237‑1 (MQ=255)

                                                                                                                  aCGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGc                                                                                                                            <  2:967808/238‑1 (MQ=255)

                                                                                                                   cGCTGGCGCAACTGGGCGCAGCGAAAATGGGGCGTCTGCGGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCgg                                                                                                                          <  2:776134/239‑1 (MQ=255)

                                                                                                                          ggaacgtggcgacgCGAAAATGTGGCGTTGGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATcaaca                                                                                                                   <  2:755022/226‑1 (MQ=255)

                                                                                                                          gcAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGAACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATcaac                                                                                                                    <  2:924083/238‑1 (MQ=255)

                                                                                                                           cAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATcaaca                                                                                                                   <  2:249939/238‑1 (MQ=255)

                                                                                                                            aaCTGGGCGCAGCGAAAATGGGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCaacaac                                                                                                                 <  2:38164/239‑1 (MQ=255)

                                                                                                                               ggtgccgatcaaaaaTTTGGCTTCTGATGCCGGGGTAGTCGAAAAAAGGCTCGATCAACAGCCTGGGCCCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGCCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACAcc                                                                                                              <  1:586249/228‑1 (MQ=255)

                                                                                                                               ggggCGCAGCGAAAATGGGGGGGTTGCCGACCGGGGAGTCAAAAAAAGCCGACATCAACAGCCGCTGCGCCC‑TGACTGGCGATCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGGAGCGGCGGA‑CGATAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACAcc                                                                                                              <  1:1066987/238‑1 (MQ=255)

                                                                                                                                     cAGGGAAAATGTGGCGTCTGCGGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCcg                                                                                                         <  2:377388/238‑1 (MQ=255)

                                                                                                                                     cAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCcg                                                                                                         <  2:868899/238‑1 (MQ=255)

                                                                                                                                     cAGCGAAAATGTGGCGGCTGCTGCTCGGTGAGGCGAAAAAAGGCTACCTCATCAGACGCGGCGCTC‑TGAATGGCGGTACGGCGCTG‑CAACAGGCGA‑AGGCGGGTATGGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCcg                                                                                                         <  1:704590/238‑1 (MQ=255)

                                                                                                                                     aaGCGAAAATGTGGCGTCTGCTGCACGGTTAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCcg                                                                                                         <  2:785925/237‑1 (MQ=255)

                                                                                                                                             aTGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGa                                                                                                                                                                                                        <  2:480820/137‑1 (MQ=255)

                                                                                                                                               ttgGCGTCCGTGGCCCGGGGGGTTGAAAAAAGGGTAAATAAACAGCCTCGGCGCAC‑TGACTGGAGGTCAGGCGCTG‑AAACGGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTAGCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATc                                                                                              <  1:1269769/238‑1 (MQ=255)

                                                                                                                                               gtgcggTCTGCTGGCCGGTGAGTGGAAAAAAGGCTACATCAACAGCCTGGGCGCAC‑GGACTGGCGGTCAGGCGTTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGGAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGTTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATc                                                                                              <  1:1085683/234‑1 (MQ=255)

                                                                                                                                               gggggcttgggtgccaggTGAGGCGAAAAAAGGCTACACCAACAGCCCGGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATc                                                                                              <  1:1250420/223‑1 (MQ=255)

                                                                                                                                                tgGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGGAATGCAGAAGTCTATCTGTCGGGGTGGCAGGGAGCGGCGGG‑CGCCTACCCGCGGGCACGCATGTAGCGGGGGCCATCGCGCTGTCCGGC‑AACACCGTGCCCACCTGTGTTGGAGGGGGCCAAAAAAACCTCCCCACGTgggactga                                                                                             >  1:1010128/1‑232 (MQ=255)

                                                                                                                                                 gggggttgctgcCCGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCCCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATc                                                                                              <  2:1268467/231‑1 (MQ=255)

                                                                                                                                                 ggCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCCACCGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATc                                                                                              <  2:1095643/237‑1 (MQ=255)

                                                                                                                                                             aCGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTcc                                                                                <  2:887283/239‑1 (MQ=255)

                                                                                                                                                               ggggAGTCGAAAAAAGGCTACACCACCAGCGTGGGCGCTC‑TGACTGGCGGGCAGGCGCTG‑CAGCAGGCGA‑AAGCGGGGATGGAAGCAGTCTAGCGGTCGGGATGGCAGGTAGCGGCGTA‑CGCTAGCCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCcgc                                                                              <  1:238170/236‑1 (MQ=255)

                                                                                                                                                               ggggAGTAGAAAAAAGGCTACATCAACAGCCTCGGCACAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCcgc                                                                              <  2:484115/236‑1 (MQ=255)

                                                                                                                                                                 tGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGGGGGGCGGGTCAACAAAACCTTCCGTCGGGCCGATCCAATCCAATGGGCCGCgg                                                                            >  2:843853/1‑239 (MQ=255)

                                                                                                                                                                        aaaaaaGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGGATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGGTCAGTCGCTCTATCCGGCAAACTCGGGG‑CCAGCTGTGGGGGAGCGGATCAACAACACCTTCCCTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTg                                                                      >  2:1166175/1‑238 (MQ=255)

                                                                                                                                                                             aGGCTACATCAAAACCCCCGGCCGCC‑TGCCTGGCGGTCAGGCGGTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCGGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCGGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCgg                                                                <  1:860730/239‑1 (MQ=255)

                                                                                                                                                                               gCTAAATCAACAGCCTCGGCGCAC‑TGGCTGGCGGTCAGGCGCTG‑CAACCGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCaa                                                                                      <  2:647747/215‑1 (MQ=255)

                                                                                                                                                                                  ccccccaccgCCCCGGCGCCG‑TGATTGTCGGTCAGGCGCTG‑CAACGGGCGA‑AAGGGGGTTTCGAAGCAGTCTGTCGTCCGTGTTGGCAGGTAGCGGCGGG‑CGCTAACCGGGGGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGAt                                                           <  1:225231/230‑1 (MQ=255)

                                                                                                                                                                                  aCATCAACAGCCTGGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGAt                                                           <  2:1007769/239‑1 (MQ=255)

                                                                                                                                                                                  aCATCAACAGCCTCGGGTCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGAt                                                           <  2:97770/239‑1 (MQ=255)

                                                                                                                                                                                  aCATCAACAGCCTCGGCGCCC‑TGACTGGCGGTAAGGCGCTG‑CAACAGGCGA‑AAGGGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGAt                                                           <  2:878829/239‑1 (MQ=255)

                                                                                                                                                                                  aCATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGAt                                                           <  2:941589/239‑1 (MQ=255)

                                                                                                                                                                                  aCATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGAt                                                           <  2:155528/239‑1 (MQ=255)

                                                                                                                                                                                         cAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGGGGAGCGGATCAACAAAACCTTCCGTCCTGCCGATCAGAACCCATGGTCCCCGGGCCTTTGGCCGGGGGGTCCccgcc                                                    >  2:27341/1‑238 (MQ=255)

                                                                                                                                                                                            ccTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTg                                                                                                    <  2:466419/188‑1 (MQ=255)

                                                                                                                                                                                             cTCGGGTCAC‑GGACTGGCGGTCCGACGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGGCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACGCCTTCCGTCGTGCCGATCAGATCCAATGGGCCGCGGGCATTGAGCCGGGCGATCCGCGCt                                                    <  2:530700/235‑1 (MQ=255)

                                                                                                                                                                                                 ggtccccgtcatggctgtgctgcggTG‑CAGCAGGGGA‑AACGGGGGAATAAAGCGGCTTAGCTGTCGGGATGGCCGGGAGCGGAGGA‑CGCAACCCTGGCGGCCAGCATGTATCAGGATCAGTCGCTCTATCCGGAAAACTAGGTG‑CCAGATGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCgt                                             <  1:1270445/218‑2 (MQ=255)

                                                                                                                                                                                                 gcgcAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGGATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGGG‑CCAGCTGTGGTGGAGCGGGTcaa                                                                                                                     >  2:673248/1‑166 (MQ=255)

                                                                                                                                                                                                  cgcAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGAt                                            <  2:981054/238‑1 (MQ=255)

                                                                                                                                                                                                      cctgccgggCGGTGGGGCGCTG‑CAACGGGCGA‑AAGCGGGTATTGAAGCAGTCTATGTGTCGGGATGGCGGGTAGCGGCGGC‑CGCTAACCGGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACGCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACt                                        <  1:27341/232‑1 (MQ=255)

                                                                                                                                                                                                      c‑tgactgGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGGATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTTGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGGTCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTTAGCCGGGGGGTCCGCGCTTTGTCGATTACt                                        >  2:422618/1‑238 (MQ=255)

                                                                                                                                                                                                        tgactgGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGGCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTc                                      <  2:1245181/239‑1 (MQ=255)

                                                                                                                                                                                                                ggTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGc                                                                                                                            <  2:608447/145‑1 (MQ=255)

                                                                                                                                                                                                                                 aGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGTGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGaa              <  2:199758/239‑1 (MQ=255)

                                                                                                                                                                                                                                     gA‑AAGGGGGTGTTTAAGTGGTCTTTCTGGCGGGCTGGCAGGTTGCGGCGGC‑AGCTTACCTGGCGGCCAGGATGTATCCGGAACAGTTCTTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGcc           <  1:237353/238‑1 (MQ=255)

                                                                                                                                                                                                                                     gA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCa                                                                                                                                                                                   <  1:378606/71‑1 (MQ=255)

                                                                                                                                                                                                                                     gA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCa                                                                                                                                                                                   >  2:378606/1‑71 (MQ=255)

                                                                                                                                                                                                                                      caaggCGGATATTTAAGCAGTCTATCTGCCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACCCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGcc           <  2:241030/234‑1 (MQ=255)

                                                                                                                                                                                                                                         aGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGGTCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCCCGCTAGGTCGATTACTTCCTGCCGATCGTTGCCGATCGGGAAGCCGGtt       >  2:1258933/1‑239 (MQ=255)

                                                                                                                                                                                                                                            gggTTTTGGAGCAGGCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGttt      <  2:1010128/237‑1 (MQ=255)

                                                                                                                                                                                                                                              gTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCCGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATAGTTGCCCATGCGGAAGCCGGTTTTggg  >  2:25365/1‑238 (MQ=255)

                                                                                                                                                                                                                                              gTATTGAAGCAGTCTATCTGGCGGGATGGCAGGTAGCGGCGGG‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTAGCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTgg   <  2:1183491/238‑1 (MQ=255)

AACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGT‑GGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCAC‑TGACTGGCGGTCAGGCGCTG‑CAACAGGCGA‑AAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGA‑CGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTG‑CCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGC  >  NC_000913/4217124‑4217596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`