BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,800	C→A	14.1%	A152S (GCG→TCG)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,800	0	C	A	14.1%	304.6 / 8.2	116	A152S (GCG→TCG)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base C (74/22); new base A (4/16); total (78/38)
Fisher's exact test for biased strand distribution p-value = 2.42e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 118 total aligned reads displayed.

CCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGG  >  NC_000913/4241562‑4242036

ccGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGc                                                                                                                                                                                                                                                    >  2:756664/1‑239 (MQ=255)

ccGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCA‑GCG‑AATA‑TG‑AA‑CTGGAGCCAGT‑TCCGc                                                                                                                                                                                                                                                    >  2:367538/1‑239 (MQ=255)

   gacgACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAat                                                                                                                                                                                                                                                 <  2:673912/237‑1 (MQ=255)

     gcacctcggccaacGGGGACTTTCGGGCCCGGTAAAAGGCAGGCTTAAGAAAAGCAGTGCAGGGGTACATGCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCTGAACGGGCAATAAAATAG‑TTTACGCAGGAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAatat                                                                                                                                                                                                                                               <  1:662535/231‑1 (MQ=255)

               caggGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCga                                                                                                                                                                                                                                     <  2:983092/239‑1 (MQ=255)

                       aCTTTCGGGCACGGTATACAGCAGGATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CAtt                                                                                                                                                                                                                            <  2:32086/238‑1 (MQ=255)

                           tCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACAGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TC‑AG‑CTGGAGCCAGT‑TCCGAAATATACATTTGCAGGA‑G‑CAtgaaggg                                                                                                                                                                                                                       >  1:999124/1‑233 (MQ=255)

                            ctggcaccgttttaggacgtcttAAAAACAGCAGTGCGGGGATCAATCCCGAGGCAAACATATAACGCCAGGCGGCAGTAATCAGGCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCt                                                                                                                                                                                                                      <  1:514718/218‑1 (MQ=255)

                                 atggTTTACAGCAGCATTAAAACCAGCAGGGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAc‑c                                                                                                                                                                                                                <  1:1275962/237‑1 (MQ=255)

                                 accggataCAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACCCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAc‑c                                                                                                                                                                                                                <  2:223055/234‑1 (MQ=255)

                                        aCAGCAGCATTAAGACCAGCAGTGCAGGGATACATCCCGAGGCAAACATATAACGCAAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCAAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAACC‑C‑AACGCCa                                                                                                                                                                                                        <  2:1076259/239‑1 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATTCATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGc                                                                                                                                                                                                                       <  1:1228130/219‑1 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGCACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑ATGGAGCCAGT‑TACGCAAGAAAAATTGGCGAAA‑G‑ACATAAAGCAAAAC‑C‑AACGCCAA‑AACCGc                                                                                                                                                                                                >  1:596308/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑CATGGTTAAAAGAGAACAGTTTACC‑GCG‑CATA‑AG‑AA‑CTGGAGCCAGT‑TATGCAATATACATTGGC‑AGAGG‑CATTGAGGCCAAAC‑C‑AACGCAAA‑AAcccc                                                                                                                                                                                                >  1:220053/1‑237 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCCATATAAATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCg                                                                                                                                                                                                 >  2:1254579/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGc                                                                                                                                                                                                                                       >  2:182338/1‑205 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTTAGGCTAAAC‑C‑AACGCCAA‑TACCg                                                                                                                                                                                                 >  2:703605/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGc                                                                                                                                                                                                                       >  2:1228130/1‑219 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGc                                                                                                                                                                                                >  2:1092720/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑AAccc                                                                                                                                                                                                 >  2:1181495/1‑237 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑AATTGAGTCTAAAC‑C‑AACGCCAA‑TACCg                                                                                                                                                                                                 >  2:671802/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATAAACATTGGCGAGA‑G‑CATTGAGGCCAAAC‑C‑AACGCCAA‑AAACGc                                                                                                                                                                                                >  1:314613/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TA‑AG‑CTGGAGCAAGT‑TCCGCAATATACCTTGGCGAGG‑G‑CATTGAGGCTAAAC‑A‑AACGCCAA‑TACCGc                                                                                                                                                                                                >  2:517906/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AAAA‑TA‑AG‑CTGGAGCCAGT‑TCCGCAATATAAATTGGCGAGA‑G‑CATTTAGGCTAAAC‑C‑AAcccccaaaaccc                                                                                                                                                                                                 >  1:376383/1‑231 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACCGGTTCAAAGAGACCAGTTTCCC‑CCG‑AAAA‑TA‑AC‑CTGAACCAAGT‑TCCCAAATAAACATT‑GCGAGA‑GCCATTGAGGCGAAAc‑a‑aaacccaa‑aacccc                                                                                                                                                                                                >  1:612351/1‑237 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTAAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑CCTGGTTAAAAGAGACCAGTTTCAC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACAATGGAGAGA‑G‑CATAGAAGCTAAAC‑C‑AAAGCACA‑TAccac                                                                                                                                                                                                >  1:490546/1‑237 (MQ=255)

                                                         agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGCGCCAGT‑TCCGCAATATACATTGGCGAGG‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCg                                                                                                                                                                                      >  2:86036/1‑239 (MQ=255)

                                                         agcagGGGAGGTAAACATTCGGGGGCAAAAATATAACGCCAGCCGTCAGTATTCAGCAAGCTGGCATCCCCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGAAA‑ACTGGTTAAAAGAGACCAGTTCCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGAAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCg                                                                                                                                                                                      <  2:376383/239‑1 (MQ=255)

                                                                cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACa                                                                                                                                                                                                                                            >  1:81469/1‑183 (MQ=255)

                                                                cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ataaata                                                                                                                                                                              >  2:103974/1‑239 (MQ=255)

                                                                cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ataaata                                                                                                                                                                              >  2:542097/1‑239 (MQ=255)

                                                                   ggATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑TTAATAAACa                                                                                                                                                                           >  2:398508/1‑239 (MQ=255)

                                                                     aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCGTCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCAGAAAATAATAGCAA‑ACTGGTTAAAAGAGACCAGTGTCCA‑GCT‑AAAC‑TG‑AA‑CTGGCGCCAGG‑TCCGACATAAACATTGGCGAAA‑C‑AATAGAGGCTAAAC‑C‑AAAGCGAA‑AACAGCCAATAAAACG‑GAAAAAAACaaa                                                                                                                                                                         >  1:635605/1‑239 (MQ=255)

                                                                     aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACaaa                                                                                                                                                                         >  2:855188/1‑239 (MQ=255)

                                                                        cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAAtt                                                                                                                                                                       >  2:174448/1‑238 (MQ=255)

                                                                        cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCCCCAATAATGCG‑ATAAAAAACAAAtt                                                                                                                                                                       >  2:1041871/1‑238 (MQ=255)

                                                                         aTTCCGAGGAAAAATAAAAACCCCAGCCGTAATTTTCCAGCCAGCTGGCATCACCGAAACGGGAAAAAAAATAG‑TTTACGAAGAAAACTAAAAGTTGCCCAAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTCCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTcc                                                                                                                                                                     <  2:965784/239‑1 (MQ=255)

                                                                             cGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCACTAATGCG‑ATAAATAACAAATTCCGGGa                                                                                                                                                                 >  2:1134142/1‑239 (MQ=255)

                                                                             cGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCAGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCTAGA‑G‑CATTGAGGCTATAA‑C‑AACGCCAA‑TACCGCCAAAAATGCG‑ATAAATAACAAATTCCCGGa                                                                                                                                                                 >  2:551238/1‑239 (MQ=255)

                                                                               aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AAAGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGaac                                                                                                                                                               >  2:525734/1‑237 (MQ=255)

                                                                               aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AAAGCCAA‑GACCGCCAAAAATGAG‑aaaaaaaaaaaaatacaggaaa                                                                                                                                                               >  1:948985/1‑229 (MQ=255)

                                                                                               ccagccgtacgttatcgaccacgtGGCCTCACCGAAACGGGAAAAAAAATAG‑TTTACGAAGTAAACTAAAAGTTCCCAAAAAATAATCGCAA‑ACTGGTTAAAAGAACCCAGTTCCCC‑GGA‑AATA‑TG‑AG‑CGGGAGCCATT‑CCCGAAATATACATTGGCGAGA‑G‑CATTGAGGCAAACC‑C‑AACCCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATaaa                                                                                                                                               <  2:490546/216‑1 (MQ=255)

                                                                                               ccAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGG‑G‑CATTTAGGCTAAAC‑C‑AACGCAAC‑TACCGCCAAT‑ATACGAATAAAAAAAAAATTCCCGAAACAAACCTGccggcaaaa                                                                                                                                               >  1:294803/1‑232 (MQ=255)

                                                                                               ccAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGCCGAGG‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTCCCAGATaaa                                                                                                                                               >  2:317517/1‑239 (MQ=255)

                                                                                                  gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑TTAAATAACAAATTCCGGGACATAACCTGCCAGTTAAACAg                                                                                                                                            >  2:520245/1‑239 (MQ=255)

                                                                                                  gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATTACAAATTCCGGGACATAACCTGCCAGATaacaag                                                                                                                                            >  2:1127185/1‑235 (MQ=255)

                                                                                                  gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAg                                                                                                                                            >  2:460648/1‑239 (MQ=255)

                                                                                                  gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAATTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑GCCGCAAAATCCATTGGCGAGC‑G‑CATTGAGGCTAAAC‑C‑ACAGCCAA‑CACCGCCAACAATGGG‑AAAAATAAAAAAATCAGGAAAAAAACCTGcacaaaaaaaag                                                                                                                                            >  1:506249/1‑228 (MQ=255)

                                                                                                        aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGAACAg                                                                                                                                      >  2:979025/1‑239 (MQ=255)

                                                                                                        aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAAA‑G‑CATTGAGGCCAAAC‑C‑AACGCCAC‑TACCGCCAATAATGCG‑ATCAATAACAAATTCCGGGACATAACCTTCCAGATAAACAGGCACAg                                                                                                                                      >  2:606504/1‑239 (MQ=255)

                                                                                                            ttCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGtgtt                                                                                                                                  >  2:1209728/1‑239 (MQ=255)

                                                                                                               caccggtgtgCATCCCCGGAAGGAGCAATAAAATAT‑TTTCAGAAGAAAACAAAAAGTTCCCAGAAAATAACAGCAA‑CCTGGTTAAAAGAG‑CCAATTTCCCGGGG‑AATA‑TG‑AG‑CGGGAGCAAGT‑CCCGAAATATACATTGGCAAGA‑A‑AATTGAGGCAAAAC‑C‑AACCCAAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                               <  1:480457/230‑1 (MQ=255)

                                                                                                               agccagcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTCCCAGATAAAAAGGCACAGtgttgt                                                                                                                                >  2:1132616/1‑238 (MQ=255)

                                                                                                                ggccagtcggaaccccGGAAAGGGAAAAAAAAAAGTTTTACGAAGAAAAAAAAAAGGGGCAAAAAAATAATCGAAA‑TGTGGTAAAAAGAACCCAGTTCCGC‑ACG‑ATTA‑TG‑AG‑AGGGAGCCAGT‑CCCACAATATAAATTGGAGAGA‑G‑AATTGAGGCTAACC‑C‑ACCCCAAA‑TTCCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                               <  1:979025/225‑1 (MQ=255)

                                                                                                                 ccagcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CCTTGAGGCTAAAA‑C‑AACGCCAA‑TACCGCCCAAAATGCG‑GAAAATAAAAAATTCCGGGGCACAACCCACCCGATAAACAGGAACAGAGTTGTACg                                                                                                                             >  1:890347/1‑239 (MQ=255)

                                                                                                                 ccagcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGAACAGAGTTGTCCg                                                                                                                             >  2:801998/1‑239 (MQ=255)

                                                                                                                  aagcTGGAAACCCCGAAACGGGAAATAAAATTG‑TTTACGAAGTAACCAAAAAGTTGCCCAAAAATAACCGCAA‑ACTGGTTAAAAGAGCCCAGTTCCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑CCCGAAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCAAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                               <  2:314613/235‑1 (MQ=255)

                                                                                                                    gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGt                                                                                                                          >  2:1075954/1‑239 (MQ=255)

                                                                                                                    gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGt                                                                                                                          >  2:480457/1‑239 (MQ=255)

                                                                                                                     cTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAACAAGGCACAGAGTTGTCCGGGtt                                                                                                                         >  2:67368/1‑239 (MQ=255)

                                                                                                                                  aaCGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGTTTATAAAGGaaaa                                                                                                             >  2:219343/1‑238 (MQ=255)

                                                                                                                                  aaCGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTACGGGACATCACCTGCCAGATAAACAGGCCCAGCGTTGTACGGGTTTATAAAGGTaaa                                                                                                             >  2:866601/1‑238 (MQ=255)

                                                                                                                                    cGGGCAAAAAAATTG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAAAAATCGCAA‑ACTGGTAAAAAGAGCCCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCACAATATACATTGGCGAGA‑G‑CATTGAGGCAAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAAcc                                                                                                          <  2:948985/239‑1 (MQ=255)

                                                                                                                                                 aG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACg                                                                                                                                                                                                           >  2:513172/1‑131 (MQ=255)

                                                                                                                                                    tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGc                                                                                           >  2:195066/1‑239 (MQ=255)

                                                                                                                                                    tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGc                                                                                           >  2:610562/1‑239 (MQ=255)

                                                                                                                                                    tttACACAATAAACTAAAGTCTGCCAGAAAATAACCGAAA‑ACTGGTAAAAAAAGCCCTGTTCCCC‑GCG‑AATA‑GG‑GG‑GTGGAGCCAGT‑CCCGAAATATAAATTGGAGAAA‑G‑AGTTGAGGCTAACC‑C‑ACCGCAAA‑TCCCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGc                                                                                           <  1:610562/239‑1 (MQ=255)

                                                                                                                                                     tttcccAGAAAAATAAAATTTGACAAAAAAAAAGCGAAA‑CCTGGTAAAAAGAAACCATTTCCCT‑GCG‑AATA‑TG‑AG‑CTGGAGCCTGT‑CCCCAAATATACATTGGAGAGA‑C‑AATTGAGACAAACC‑C‑AACCCAAT‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                          <  1:1092720/234‑1 (MQ=255)

                                                                                                                                                     ttccGCAGTAAACTAAAGTTTCCCCGAAAATAACCGCAA‑ACTGGTTAAAAAAAACCGTTTCCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑CCCGCAATATACATTGGCGAAA‑G‑CATGGAGGTTAACC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                          <  2:652595/236‑1 (MQ=255)

                                                                                                                                                      ttaccatgtaaCTAAAAGTTTGCCAAAAATAAACGCAA‑TGTGGGTAAAAAGAACCCGTTTCCC‑GGG‑GAAT‑TG‑TGCCTGGACCCGGT‑CCCGAATAATAGTTGGGCGAGC‑G‑ATTTGAGGCTAACC‑C‑AACGCCAT‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                          <  1:174448/230‑1 (MQ=255)

                                                                                                                                                      tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGCCATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGGTTATAGAGGTACAACCAAGTTCTGGCCAGGCAg                                                                                         >  2:1128055/1‑239 (MQ=255)

                                                                                                                                                      tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAg                                                                                         >  2:688712/1‑239 (MQ=255)

                                                                                                                                                      tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGTTTATAAAGGTAAAACCAAGTTCTGGCCAGGccg                                                                                         >  2:1284223/1‑237 (MQ=255)

                                                                                                                                                      tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGAGTTGTCCGGGTTTATAAAGGTAAAACCAAGGTCTGGCCAGGcgg                                                                                         >  2:1160346/1‑237 (MQ=255)

                                                                                                                                                      tACGCAGTAAACTAAAAGATGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGTCGAGA‑G‑CATTGAGGATAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACCTAACCTGCCCGATAAACATGCCAAGTGTTGTCCGGGTTTAAAAAGGAAAAACCaatgttcggccaagaaa                                                                                         >  1:665844/1‑224 (MQ=255)

                                                                                                                                                        cGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCgag                                                                                                                                                                                                                                    <  2:864408/104‑1 (MQ=255)

                                                                                                                                                        cGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCgag                                                                                                                                                                                                                                    >  1:864408/1‑104 (MQ=255)

                                                                                                                                                         gCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGaa                                                                                       >  2:824524/1‑238 (MQ=255)

                                                                                                                                                            gTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAatat                                                                                                                                                                                                                                               >  1:50770/1‑89 (MQ=255)

                                                                                                                                                              aaaCTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTGGTCCGGGCTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTac                                                                                  >  2:304938/1‑238 (MQ=255)

                                                                                                                                                                  aaaagtttcacaaaaaaaaACCGCAA‑AGGGTTTAAAAGAGCCCGAGGTCCC‑GAA‑AATA‑TG‑AG‑CGGAAGCAAGT‑ACCAAAATAGCAAGTGGCGAAA‑G‑CATGGAGGCAAAAC‑C‑AACGCAAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                             <  1:703605/226‑1 (MQ=255)

                                                                                                                                                                   aaaaATTGCCCCAAAAAAATTCCAATACTGATTAAAAGACACTATTTACCC‑G‑G‑CATAGTG‑AG‑TTGGAGATAGT‑TCCGAAATATCAATTGGCGAAC‑G‑CATTGGGACTAAAC‑C‑ATCGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                             <  2:1247777/239‑1 (MQ=255)

                                                                                                                                                                          gCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAACAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGTCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATACG‑ATAAAAAACAAATTCCGGGACATGACCTGCCAGATAAACAGGAACAGTGTTGTCCGGGGTACTAGAGGTAAAAACAAGGTGTTGCCCGGCCGAACCTAAAACAAAAATaaaa                                                                     >  1:1131199/1‑239 (MQ=255)

                                                                                                                                                                           cccGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCCGAAAAACAAGCACAAGGTTGTCCGGGTTTATAAAGGGAAAACCAAGTTCTGGCCAGGCGAAACCCACACCAGAaaaaaaaa                                                                    >  1:990320/1‑239 (MQ=255)

                                                                                                                                                                           cccGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATCAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaa                                                                    >  2:778087/1‑239 (MQ=255)

                                                                                                                                                                           cccGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaa                                                                    >  2:627860/1‑239 (MQ=255)

                                                                                                                                                                                 aatacccGCAA‑ACTGGTTAAAACAGACCGGTTCCCC‑GGA‑AATT‑TG‑GC‑GAGGACCCAGT‑GCCGAAATATCACGTGGGGAGA‑G‑CTTTGGGTCCACAC‑C‑GACGCCAA‑ATCCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                              <  1:756084/233‑1 (MQ=255)

                                                                                                                                                                                  aaaaaagcgaaaaaggTGAAAAGAGACCCGATTCCC‑GAGAAATT‑TG‑CG‑CGGCAGCAAGT‑CCCGCAAGATACAGTGGGGGAA‑G‑AATTGA‑GATAAAA‑C‑GACCCCAACTACCGCTAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAg                                                                <  1:278132/224‑1 (MQ=255)

                                                                                                                                                                                    aatCGAAA‑ATTGGTTAAAAGAGACCAGTTCCCC‑G‑G‑AATA‑TGTGG‑GTGGGCCCAGT‑TCCGCAATAACCATTGGCGAGA‑A‑CATTGAGGCTAACC‑C‑AACCCAAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcag                                                           <  2:596308/239‑1 (MQ=255)

                                                                                                                                                                                       caaaa‑aCTGGTAAAAAGAGACCATTTCCCC‑GCA‑AATT‑GG‑GG‑CTGGAGCCATT‑TCCGAAATATACATGGGCGAAA‑G‑CATTGAGGCAAACC‑C‑AACGCAAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagcag                                                        <  2:890347/236‑1 (MQ=255)

                                                                                                                                                                                        ccacacgtgttaaaaaGACACCAG‑TTCCC‑GGG‑GAAA‑TG‑GG‑GTGGGGCCCGTGCCCGAAATATACATTGGGGAGG‑G‑CATTGA‑GCAAAACAC‑AACGCAAA‑AACCGCCAATAAGGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagcag                                                        <  1:1041871/227‑1 (MQ=255)

                                                                                                                                                                                                           aCCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAC‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGCTAACCAGGCCCAGTGTTGTCCGGGTTTATCGCGGTAAAACCAAGTCCTGACCAGGCAATCCCTCCCCCAGAACAAAAACAAAGAAACACACCAATCTTaacggaaaccaa                                     >  1:616519/1‑229 (MQ=255)

                                                                                                                                                                                                           aCCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAATTGAATCacg                                     >  2:242977/1‑239 (MQ=255)

                                                                                                                                                                                                           aCCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCacg                                     >  2:805562/1‑239 (MQ=255)

                                                                                                                                                                                                                tttCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACgacgac                                >  2:614581/1‑239 (MQ=255)

                                                                                                                                                                                                                   ccc‑GCG‑AATA‑TG‑AG‑ATGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATATGGGTAAAACCAAGTTCTGGCCAGGAAGAACCTACACCAGAAATAAAAAACAGGACAGCACCAATCTTAGGGGAATAACGACGACCGa                             >  2:8289/1‑239 (MQ=255)

                                                                                                                                                                                                                            ata‑tG‑AG‑GTGGAGCCAGT‑CCCGAAAATTACTTTGGGGAGA‑C‑AATTGAGGCTAAAC‑C‑AACCCAAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                       <  2:635605/238‑1 (MQ=255)

                                                                                                                                                                                                                                        gaaaccggT‑TCCAAAATATGAATTGGGGAGA‑G‑‑ATTGGAGGTAAAC‑CAAACGCCAA‑AACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAAGAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAAt              <  2:406767/231‑1 (MQ=255)

                                                                                                                                                                                                                                              aGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTAAACCCGAAATAAAAAACAGGCCAGAAGCAATCTTAAGTGAATCACAACGACCGAACAGGTTACAGAAAAAaccaa        >  1:1023414/1‑237 (MQ=255)

                                                                                                                                                                                                                                                    cGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAGATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTACGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAACAATCTTAATGAAATCACGACAACCAAAACGATAAATGCAAAAAAAACCAAggg  >  1:490370/1‑239 (MQ=255)

CCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGACCAGTTTCCC‑GCG‑AATA‑TG‑AG‑CTGGAGCCAGT‑TCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAC‑C‑AACGCCAA‑TACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGG  >  NC_000913/4241562‑4242036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`