BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,993	T→G	14.7%	S87S (TCA→TCC)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,993	0	T	G	14.7%	248.1 / 7.5	120	S87S (TCA→TCC)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base T (64/34); new base G (9/11); total (73/47)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGA  >  NC_000913/4241763‑4242220

aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAATT‑GAATCacg                                                                                                                                                                                                                                >  2:242977/1‑239 (MQ=255)

     tttCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACgacgac                                                                                                                                                                                                                           >  2:614581/1‑239 (MQ=255)

        cccGCGAATATGAGATGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATATGGGTAAAA‑CCAAGTTCTGGCCAGGAAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCACCAATCTTAGGG‑GAATAACGACGACCGa                                                                                                                                                                                                                        >  2:8289/1‑239 (MQ=255)

               atatGAGGTGGAGCCAGTCCCGAAAATTACTTTGGGGAGACAATTGAGGCTAAA‑CC‑AACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGt                                                                                                                                                                                                                  <  2:635605/238‑1 (MQ=255)

                        gaaaccggTTCCAAAATATGAATTGGGGAGAG‑ATTGGAGGTAAA‑CCAAACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAAGAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAAt                                                                                                                                                                                                         <  2:406767/231‑1 (MQ=255)

                         ttggtaaaagaaacggataCATGGGGGAGGGAATGGGGGCAAAC‑CC‑AACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                                                                                       <  2:33529/223‑1 (MQ=255)

                            caggtccgacaataaaattgggCGAGAGCATTGAGACTAAC‑CA‑AACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAacc                                                                                                                                                                                                     <  2:612351/218‑1 (MQ=255)

                              aGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTAAACCCGAAATAAAAAAC‑AGGCCAGAAGCAATCTTAAGT‑GAATCACAACGACCGAACAGGTTACAGAAAAAaccaa                                                                                                                                                                                                   >  1:1023414/1‑237 (MQ=255)

                                             aTTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAAACTCAACCAGAAATAAAAAAC‑AGGACAGCAGACATCTTAAGT‑GAATAACGACGAACGAAGCGGTTACTGCAAAAAACACCAAGGGCAcccccgga                                                                                                                                                                                   >  1:995924/1‑237 (MQ=255)

                                             aTTGCCGATAGCATTCAGGCTAAA‑CC‑AACGCCAATACCGCCAATACTGCGATCAATACCAAATTCCGGG‑C‑CATAACCTGCCCGTTATCCAGGCAAAGAG‑TTGTCCGGGTTC‑ATAAAGGAAAAA‑CCACGTCCTGGCCAGGCCGAACCTACACCCGAAATAAAAAAA‑CGCCCAGGCGCAACCTTACAT‑CAATCACCACAACCAAAGAGGTGCCTGCCAAAaccaccacgccaaccccaccc                                                                                                                                                                                   >  1:1018346/1‑224 (MQ=255)

                                               tGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCCCGACGACCGAAGCGGTTACTGCAAAAACCACCGAGGGCAACGCCgatga                                                                                                                                                                                 >  1:612085/1‑239 (MQ=255)

                                               tGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                                                                                                 >  2:997382/1‑239 (MQ=255)

                                                  cgatgtctgtgaagcacaa‑CC‑AGCGACAATCCCGCCAATATGGCGATAAATAACAAATTCCGGT‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAC‑CCAAGTTCTGGCCAGGCAGAACCTACCCCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGACACCGCCGatgatg                                                                                                                                                                               <  2:220053/221‑1 (MQ=255)

                                                  cGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCCGAACCTACACCAGAAATAAAAAAC‑AGGAAAGCAGCACTCTTAAGT‑GAATCACGCCGACCGAAACGGTTAATGCAAAAACCACCCAAGGCACCCCCgataaggc                                                                                                                                                                              >  1:914518/1‑234 (MQ=255)

                                                  cGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACAACGACCGAAGCGGTTACTGCAATAACCACGCAGGGCACCGCCgatgtagc                                                                                                                                                                              >  2:637969/1‑235 (MQ=255)

                                                  cGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACAGACAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGACAAATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTAAACAAGAAATAAAAAAC‑AGGACAGCAGAAATCTAAAGG‑GAATCACGACGACCGAAGCAGTTACTGAAAAAACCACAGAgggaaacaccggcagacg                                                                                                                                                                              >  1:514725/1‑224 (MQ=255)

                                                   gggagTATTGGGGGTAAACCC‑AACCCAAATACCGCAAATAAGGCGATAAATAACAAATTCCGGG‑A‑AATAACCGGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                                                                                                                                               <  2:112563/236‑1 (MQ=255)

                                                    agagCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCCGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGCCGACCAAAAAGGTTACTGCAAAAACCACCGAGGGAACCGCCCATGAGGCaa                                                                                                                                                                            >  1:146755/1‑239 (MQ=255)

                                                    agagCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGA‑A‑CATAACCTGCCAGATAAACAGGCACAGGG‑TTGTCCGGGTTT‑ATAGAGCTCAAA‑CCAAGTTCTGGCCAGGCAGAAACTACACCACAAATAAAAAAC‑AGAACAGAGGCAATCTTAAGT‑GAATCACGACGACCGAAGCGTATACGGCAAAAAAAACCGAAGGcccggctctaataaaaa                                                                                                                                                                            >  1:33529/1‑223 (MQ=255)

                                                      agCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAATAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGCAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAcc                                                                                                                                                                          >  2:632225/1‑239 (MQ=255)

                                                      agCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAACCTTAAGG‑AAATCCCGACGACCGAAGCGGTTACTGCAATAACCACCAAGGGCACCGCCGATGATCAAAcc                                                                                                                                                                          >  1:1023108/1‑239 (MQ=255)

                                                           tGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCa                                                                                                                                                                     >  2:1247864/1‑239 (MQ=255)

                                                           tGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGAACCGCCGATGATGCAACCAATCa                                                                                                                                                                     >  2:373602/1‑239 (MQ=255)

                                                                aaaaa‑CC‑AACCCAAATCCCGCCAATAATGCGATAAATAAAAAATTCCGGG‑A‑CATAACCTGCCAGAAAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAAAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgcg                                                                                                                                                                <  2:1023108/237‑1 (MQ=255)

                                                                  ccccaaccccgacaaccccGCTAATAGTGACAAAAATAACCATGTCCGGC‑A‑CATAACCTGCCAGATAAATGGGCACGTTG‑TTGCCAGGTTGT‑AGAAAGGAAAAC‑ACAAGCTCGTGCCAGGAAGACTCAAC‑CCAAAAAAAAAAAACAAGGCCGGCAGC‑ATTTTAACTGGAACCCGGACGACCGAAGCGGTTCCGGCCATAGCCACCGACGGAACCGCCGATGATGCAACCAATCAGAgcg                                                                                                                                                                <  1:1125159/222‑1 (MQ=255)

                                                                    a‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCCAAGCGCTg                                                                                                                                                             >  2:27840/1‑238 (MQ=255)

                                                                           cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACg                                                                                                                                                       >  2:482197/1‑239 (MQ=255)

                                                                           cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCAAGGGCACCCCCGATGATGCAACCAATCagctggctcttttac                                                                                                                                                       >  2:558990/1‑226 (MQ=255)

                                                                              aaagcccGCCAAAAATGCAAAAAAAAAAAAATTCCGGG‑A‑AATAACCTGCCAGAAAAACAGGCACATTG‑TTGTCGGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                    <  2:1023414/234‑1 (MQ=255)

                                                                               caaccccccAAAAAGGGGAAAAAAAAAAAATGCCGGG‑ATCTAAACCAGCAAGA‑AAACAGGCACAGGGTTTGCCCGGGTTA‑AGAAGGGAAAAA‑AAAATTCCGGGACAGGAAAGACAAACACAAAAAAAAAAAACC‑AGGACGACAACAATTAGGAGG‑GAACACCGACGCACGAGGCGTTGAAGGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                    <  1:1181495/232‑1 (MQ=255)

                                                                                aTACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACCGGCACAGTG‑TTGTCCGGGCTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCCATAACTACCGAGGTCACCGCCGCAAATGCAACCAATCagacccattcccaccgaaa                                                                                                                                                   >  2:474982/1‑222 (MQ=255)

                                                                                             tGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCAGCCAAAACCCTAACAGGGAg                                                                                                                                     >  2:258847/1‑239 (MQ=255)

                                                                                             agcggataataaAAAAATCCGGGAA‑CAAATCCTGCCAAAAAAACAGGCA‑AGTG‑GTTTCCGGGTTTAATAGAGAAAAAC‑CAAAGTCCGGGCAAGGGAGAACCTACACCAGAAAAAAAAAAC‑AGGCCAGCACCAATCTTAAGG‑GACCCACGAGGACGGAGGCGTTTACTGCAATAACCACGGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGa                                                                                                                                      <  1:888931/232‑1 (MQ=255)

                                                                                               ggATAAATAAAAAATCCGGGG‑A‑AATAACCGGACAGAAAAAAAGGCACAGTG‑TTGGCGGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCGGGCAAGGAAGAACATACACCAGAAATAAAAAAC‑AGGACAGCAGAAAGCTAAAGG‑GAAGCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAg                                                                                                                                     <  2:616519/236‑1 (MQ=255)

                                                                                                  taaataaCAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑ATGTCCGGGTTT‑ATAGAGGAAAAA‑CCAAGTTAAGGCCAGGCAGAACCTACCCCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCAAGACAACAGCAGCCGTTACTCAAATAACCACAGAGGGCACCGCCGCTCATGCACACAATAAGAGCACAGGCCACCCAAACACCTAACACGGAGTTgag                                                                                                                                >  1:134604/1‑237 (MQ=255)

                                                                                                        aCAAATTCCGGG‑A‑AAAAACCTGCCAAATAAACAGGCACAGTT‑TTGTCCGGTTTT‑ATAGAGTTAAAC‑CCAAGTTCGGGCCAGGCAGAACCTACCCCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGCCCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGa                                                                                                                          <  2:914518/239‑1 (MQ=255)

                                                                                                         cAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGCTGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGcgcaggat                                                                                                                         >  2:1103920/1‑232 (MQ=255)

                                                                                                          aaaTCCCGGC‑A‑CATACCCTGCCAGAAAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAC‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGAtt                                                                                                                        <  2:1127663/239‑1 (MQ=255)

                                                                                                            aTTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCAAGGGCACCGCCGAGGAGGCAACCCCTCAGAGCCCTGGCCCCGAACAACACAAAAAGGGAGAGGGCAGGGGATACa                                                                                                                      >  1:965325/1‑239 (MQ=255)

                                                                                                                            ccTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTgg                                                                                                       >  2:489521/1‑239 (MQ=255)

                                                                                                                               gCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCCAGGGCACCGCCGGTAATGCACCCAATCAGCGCGCTGGACACGCAACACCATAACCGGGAGTTGGCAGCGGAttacctagagtttgtgggaga                                                                                                     >  1:1093498/1‑220 (MQ=255)

                                                                                                                                  aaaaaaccgggCACGGTG‑TTGTCCGGGTTT‑AAAGAGGAAAAC‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACGGCAGCAATCTTAAGG‑GAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGcaac                                                                                                 <  2:429288/230‑1 (MQ=255)

                                                                                                                                    aTAAACAGGCACAGTT‑TTGTCCGGGTTT‑ATAGAGGTAAAC‑CCAAGTTCGGGCCAGGCAAAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGcaaca‑a                                                                                              <  2:612085/239‑1 (MQ=255)

                                                                                                                                    aTAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCCATAACCACCGAGGGAACAGCCGATGCTCCACCCAATCAGAGCGCTGGCCCAGAAAAACCCACACAGGGAGTGGGCAAAGGATTAAC‑ATAAGTTTTGAGGaaaaaaaa                                                                                               >  1:129559/1‑232 (MQ=255)

                                                                                                                                    aTAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGCAGTTGGCAGCGACTTCAC‑TTAAGTTTTGTGGAGcaaca‑a                                                                                              >  2:1170031/1‑239 (MQ=255)

                                                                                                                                    aTAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGAAAAA‑CCAAGTTCTGGCCAGGCAGAACATACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGATTACGGCAATAACAACCGAGGGGACCACCGATGATGCAACCAATAGTACCACGGGCACGGCGAAACCCTAACAGGGAGCTGGCAGGGGATTAAC‑ATAAATTTTGTGGAGcaaaa‑a                                                                                              >  1:81597/1‑239 (MQ=255)

                                                                                                                                       acacgggcaggtg‑tGGTCGGGGTTT‑AAAGAGTTAAAA‑CCAATTTCTGGCCAGGACAACCCTACACCAGAAATAAAAACC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGa                                                                                           <  2:806521/229‑1 (MQ=255)

                                                                                                                                            gCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCATCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACggga                                                                                      >  2:997057/1‑237 (MQ=255)

                                                                                                                                              acaGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATAAGGGCTCTGGCCACGCAAAACCCTAACAGCGAGTTGGCAGAGGATTCAC‑TTCAGTTTTTGGGGGAACCA‑AAAACgggaat                                                                                    >  1:87325/1‑235 (MQ=255)

                                                                                                                                                 ttg‑ttgtCCGGGTTG‑AAAAGGGTAAAA‑CCAACTTCTGGCCAGGCAAAACCAACCCCAAAAAAAAAAAAC‑AGGACAGTAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAg                                                                                <  2:1131199/238‑1 (MQ=255)

                                                                                                                                                  tg‑ttgtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGAATT‑GAgg                                                                               >  2:90867/1‑238 (MQ=255)

                                                                                                                                                    tttgtCCGGGTTA‑AAAGGGGAAAAA‑CCAAGTTCGGGCCAGGAAGACCCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCATAATT‑GAATCACGACGCCCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGt                                                                               <  2:506249/237‑1 (MQ=255)

                                                                                                                                                       gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGGTTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGCCGGTATT‑GAGTGAc                                                                            >  2:565166/1‑238 (MQ=255)

                                                                                                                                                       gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAAACGGAATT‑GAGTGACt                                                                           >  2:524795/1‑239 (MQ=255)

                                                                                                                                                       gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAAACGGAATT‑GAGGGACt                                                                           >  2:996128/1‑239 (MQ=255)

                                                                                                                                                       gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAACAGCAATCTTAAGT‑GAATCACGCAGACCGAATCGGTTACTGCATTAACCACCGAGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGAAAAACCCACAAAGGCGGTTGGAACCGGAATCAC‑TAAAGTTTTTGGGAGAAAAA‑AAAACggaaataggggagt                                                                            >  1:481462/1‑227 (MQ=255)

                                                                                                                                                        tCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACACAAAACCCTAACAGGGAGTTGGGAGCGGATTCAC‑TTAAGTTTTGTGGAACAACA‑AAGACGGTAAT‑GAGTGaacc                                                                          >  1:716570/1‑236 (MQ=255)

                                                                                                                                                           cgttttttaaggtgaaaaa‑CAAATTACTGCCCAGGAAAACCCTACCCCAGAAAAGAAAAAA‑GAGACAGCGAAAATCTGAAGT‑GAATCACGACGCCCGAGGCGGTAACTGAAGTAGCCACAGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCGAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCaa                                                                        <  1:630485/224‑1 (MQ=255)

                                                                                                                                                            cggttttaaaagGTAAAACCCAAGTTCTGGCCGGGCGAAACCTACACCAAAAAAAAAAAAC‑GGTACAGCAGCAAGCTTAAGT‑GAACCCCCACGACCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCaa                                                                        <  2:134604/229‑1 (MQ=255)

                                                                                                                                                             gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCCGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGAAGACCGAAGCGGTTACTGTAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCACAAGCCTGGCCAAGCAATACCCCAACAGGGGGTTGGTAGCGGCTTCAC‑TTAAATTTTGTCGAGCAACA‑AAGACGGTATT‑GAggagccaaacaa                                                                     >  1:704444/1‑228 (MQ=255)

                                                                                                                                                             gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCCATCTTAAGT‑GAATCACGACGACCGAAGCGGTTATTGCAATAACCACCGAGGGACCCGCCGACGGTGCCACCAATCAGAGCGCTGGCCAAGCAAAACCCCAACAGGGAGTTGGCGGCGGA‑TCACATTAAGTTTTTGGGAGAAAAA‑AAAACGGGAtgggaggaccaaaaag                                                                      >  1:683109/1‑224 (MQ=255)

                                                                                                                                                             gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTCACAGGGAGTTGGCAGCGGATTCCC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTGTT‑GAGTGACTCAACAg                                                                     >  2:1045963/1‑239 (MQ=255)

                                                                                                                                                             gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGGGCAACA‑AAGACGGAATT‑GGGTGACCCAACaa                                                                     >  2:1024795/1‑238 (MQ=255)

                                                                                                                                                             gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAg                                                                     >  2:619949/1‑239 (MQ=255)

                                                                                                                                                             gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCCAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCTAAACCCTAACCGGCGGTTGGCAGCGGATTCCC‑TTAAGTTTTGTGGAGCACCA‑AAGAGGGTATT‑GAGTGCCTCAAAAg                                                                     >  2:1227995/1‑239 (MQ=255)

                                                                                                                                                             gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGAAAGCAGCAATCTTAAGT‑GAATCACGACGACGGAACCGGTTACTGCATCAACCACCGAGGGCACCGCCGTTAATGCAACCAATCAGAGGACCGCCCTCGCAAAACCCCAACAGGAGGTTGGCACCGGATCCAT‑TTAAGTTTTTTGGTGTAAAA‑AAAACGGTAttaggagatcatatac                                                                      >  1:1028401/1‑225 (MQ=255)

                                                                                                                                                                  ataGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCC‑TTAAGTTTTGTGGAACAAAA‑AAAACGATAttaaagaacacaaaagaaac                                                                  >  1:23906/1‑221 (MQ=255)

                                                                                                                                                                   taGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCg                                                                >  2:796930/1‑239 (MQ=255)

                                                                                                                                                                        gTAAAA‑CCAAGTTCTGGCCCGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATCATGCAACCAATCCGAGCGCTGGCCACGCCAAACCCTAACAGGGAGGTGGCAGCAGCATCAC‑TTAAACTTTGTGGAGAAACA‑ACAACAGCATT‑GAGTGTATCAACAAAAACGGAAAt                                                           >  1:450832/1‑239 (MQ=255)

                                                                                                                                                                        gTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAGAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGACGCGGTTACGGCAATAACCACCGAGGGCAACGCCGAGGATCCAACCAATCAGAGCGCCTGCCACGCCACACCCTAAACGGGCGTTGGCCGCCGGACCAC‑TTACGCTTTGTGGCGCCACACAAAACGG‑ATT‑GGGGGACTCacaaaacacgcaaat                                                           >  1:110459/1‑225 (MQ=255)

                                                                                                                                                                        gTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GACTCACGACGACCGAAGCGGTTACTGCAATAACAACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCCCGCAAAACCCTAACAGGGCGTTGGCAGCGGATTCCA‑TTAAGTTTTGTGGAGCAACA‑AAGACGGAATT‑GAGTGACTCAAACGTACCGGAAAt                                                           >  2:488848/1‑239 (MQ=255)

                                                                                                                                                                        gTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACACTAACAGGGAGTTGGCAGCCGATTCAC‑TTAAGTTTTGTGGCGCAACA‑AAAGAGGTATT‑GAGGGAATCAAAATAACAGGaaaa                                                           >  1:441094/1‑238 (MQ=255)

                                                                                                                                                                         gaaaa‑CCAAGTTCTGGCCAGGCAGAACATACACAAGTAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATTGCGACGACCGAAGCGGATACTGCAATAATCACCGAGGGCACCGCCGATGATGGAACAAATCAGAGGGCTGGCCACTCAAAACCCGCACCTGGGTCGGGCAGCGGAGTCAC‑TTAAGGTTTGTGAAGCCACA‑AAAAAGGTATT‑TAGCGACTTCACATAAACGGaaaaa                                                          >  1:269760/2‑237 (MQ=255)

                                                                                                                                                                          aaac‑CCAAGTTCTGGCCAGGCAGAACCTACACCAAAAAAAAAAAAC‑AGGCCAGCAGCAATTTTAAGT‑GAACCACGACGGCCGAAGCGGTTACGGAAATAACCCCCGAGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATaa                                                         <  2:995924/239‑1 (MQ=255)

                                                                                                                                                                                cAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGCCAGCAGCAATCTTAAGT‑GAACCACGACGACCGAAGCGGTTACTGAAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAAcggcg                                                    <  2:822374/239‑1 (MQ=255)

                                                                                                                                                                                  aGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACCGGGAGTTGGCAGCGGCGTCAC‑TTAAGTTTTTTGGAGCAAAA‑AAGACGGAATT‑GAGTGACTCAACCTTACCGGAAATAACGGCGgt                                                  >  2:714248/1‑239 (MQ=255)

                                                                                                                                                                                   gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCC‑TTCAGTTTTATGGAGCAACA‑AAAACGGTATT‑GAGCGACTCAACAGTACCGGCAATAAaggcggag                                                 >  2:1249206/1‑237 (MQ=255)

                                                                                                                                                                                   gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGGGACTCAACAGTACCGGAAATAAcggcgggt                                                 >  2:946903/1‑237 (MQ=255)

                                                                                                                                                                                    gtcgggacAGGAAAAACATACACCAGAAAAAAAAAAC‑AGGACAGCAGAAATCTTAAGG‑AAACCACGACGCCCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGgtgt                                                <  2:1093498/232‑1 (MQ=255)

                                                                                                                                                                                        ggCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAACAATCAGAGCGCTGGCCACGCCAAACCCTAACAGGGAGTTAGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGTTATT‑GAGTGACTACACAAGAACGGAAAACAcggcgggatggag                                            >  1:812509/1‑232 (MQ=255)

                                                                                                                                                                                         gCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACTCAAAACCCTAACAGGGAGTTGGCAGCGTATTCAC‑TTAAGTTTTGTGGCGCAAAA‑AAGACGGTATTAGAG‑GACGCAACACAACCGGAAATAAcgtcgggggtggac                                           >  1:1019353/1‑231 (MQ=255)

                                                                                                                                                                                         gCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGgttgccgt                                            >  2:190449/1‑232 (MQ=255)

                                                                                                                                                                                           cggcgaacaCCTACCACAAAAAAAAAAAGC‑AAGACAGCAGAACTTATAAGT‑GAACCCGCACGACCGAAGGGTTTACGGAAACACCCACCGAGGCACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGcc                                         <  1:489521/231‑1 (MQ=255)

                                                                                                                                                                                              gCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGCGGGCACCGCCGATGATGCAACCAATCTGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGGGCAACA‑AAGACGGTATT‑GAGTGACTCAACCGTACCGGAAATAACGCCGgtgcgtgctgccaa                                      >  2:1137047/1‑228 (MQ=255)

                                                                                                                                                                                              gCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACt                                                                           >  2:954687/1‑202 (MQ=255)

                                                                                                                                                                                                   aCCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACa                                                                      >  2:700086/1‑202 (MQ=255)

                                                                                                                                                                                                     ttaacacaaaaaGAAACAAC‑AGGACATCAGCAATCTTAAGT‑GAACCAGGCCGACCGAAGCGGTTACTGAAAAAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                               <  2:110459/230‑1 (MQ=255)

                                                                                                                                                                                                     tcaccccAGAAAAAAAAAAC‑AGGACAGCAGCAACCTTAAGT‑AAATCACGCTGACCGAAGCGGTTACTGCAATAACCACGGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                               <  2:81597/234‑1 (MQ=255)

                                                                                                                                                                                                     cTACACCAAAAAAAAAAAAC‑AGGACAGCAGCAATCTAAAGT‑GAACCCCGCAAACCGAAGCGGTTACTGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                               <  2:683109/239‑1 (MQ=255)

                                                                                                                                                                                                     accccccAAAAAAAAAATAC‑AGAACACCGTCAATCTGAAGC‑GACCCCCGACACCAGAAGCGTGTACTCCAATCCCCCCCGAGGCGACCGCGGATGGGGCACCCAATCAGAGCGCTGGCCACGCAAAACACTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                               <  1:671802/234‑1 (MQ=255)

                                                                                                                                                                                                       ggctgggagatgtgtaaaagaaaCAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                               <  2:943887/216‑1 (MQ=255)

                                                                                                                                                                                                        cacCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGGGGCGTAGCCAAAAAATaaaaaa                            >  2:480191/1‑236 (MQ=255)

                                                                                                                                                                                                                 aaaaaacC‑AAGACACCAGCAAACATAATG‑TAACCACCGCGACCGAAGGGGTTCCTTCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTa                   <  2:514725/238‑1 (MQ=255)

                                                                                                                                                                                                                 aaaaaaaC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACCGAAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                               <  2:10672/226‑1 (MQ=255)

                                                                                                                                                                                                                            gACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGGGACTCAACATTACCGGAAATAAGGGCGGGGGCGGTGTCGAATAATAaacaacaaaaagacagtctactaa         >  1:10672/1‑221 (MQ=255)

                                                                                                                                                                                                                            gACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGAATT‑GAg                                                                                >  2:654154/1‑168 (MQ=255)

                                                                                                                                                                                                                                 cagcaATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGGGTCGTAGCCCAAAAATAAACCACCTACTTTTGGGACCAAGGCaaac    >  2:873067/1‑237 (MQ=255)

                                                                                                                                                                                                                                  agcaATCGTAGGT‑GAATCACGACGACAGAGGCGGTTACTGAAAGAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAAt     <  2:700261/237‑1 (MQ=255)

                                                                                                                                                                                                                                  agaaaTCTAAAGG‑GAACCCCGACGACCAAAGGGGTTACTGAAAAACCCCCCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTa       <  2:1019353/232‑1 (MQ=255)

                                                                                                                                                                                                                                     aaTCTTAAGT‑GAATCCGGTCGGCCGAAGGGGTTACCGCAAAAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGa  <  2:129559/237‑1 (MQ=255)

ACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TTGTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACA‑AAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGA  >  NC_000913/4241763‑4242220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`