BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,242,021	C→A	11.7%	C78F (TGC→TTC)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,242,021	0	C	A	11.7%	290.9 / 2.3	128	C78F (TGC→TTC)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base C (68/41); new base A (3/15); total (72/56)
Fisher's exact test for biased strand distribution p-value = 5.07e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGAC  >  NC_000913/4241787‑4242259

gaaaccggTTCCAAAATATGAATTGGGGAGAG‑ATTGGAGGTAAA‑CCAAACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAAGAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAAt                                                                                                                                                                                                                                               <  2:406767/231‑1 (MQ=255)

 ttggtaaaagaaacggataCATGGGGGAGGGAATGGGGGCAAAC‑CC‑AACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                                                                                                                             <  2:33529/223‑1 (MQ=255)

    caggtccgacaataaaattgggCGAGAGCATTGAGACTAAC‑CA‑AACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAacc                                                                                                                                                                                                                                           <  2:612351/218‑1 (MQ=255)

      aGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTAAACCCGAAATAAAAAAC‑AGGCCAGAAGCAATCTTAAGT‑GAATCACAACGACCGAACAGGTTACAGAAAAAaccaa                                                                                                                                                                                                                                         >  1:1023414/1‑237 (MQ=255)

           cGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAGATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTACGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAACAATCTTAATG‑AAATCACGACAACCAAAACGATAAATGCAAAAAAAACCAAggg                                                                                                                                                                                                                                   >  1:490370/1‑239 (MQ=255)

                 ataCATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCACGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAACAACCAACAAGGGCAcacc                                                                                                                                                                                                                             >  2:586862/1‑236 (MQ=255)

                     aTTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCcat                                                                                                                                                                                                                         >  2:1192898/1‑239 (MQ=255)

                     aTTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAAACTCAACCAGAAATAAAAAAC‑AGGACAGCAGACATCTTAAGT‑GAATAACGACGAACGAAGCGGTTACTGCAAAAAACACCAAGGGCAcccccgga                                                                                                                                                                                                                         >  1:995924/1‑237 (MQ=255)

                     aTTGCCGATAGCATTCAGGCTAAA‑CC‑AACGCCAATACCGCCAATACTGCGATCAATACCAAATTCCGGG‑C‑CATAACCTGCCCGTTATCCA‑GGCAAAGAG‑TT‑GTCCGGGTTC‑ATAAAGGAAAAA‑CCACGTCCTGGCCAGGCCGAACCTACACCCGAAATAAAAAAA‑CGCCCAGGCGCAACCTTACAT‑CAATCACCACAACCAAAGAGGTGCCTGCCAAAaccaccacgccaaccccaccc                                                                                                                                                                                                                         >  1:1018346/1‑224 (MQ=255)

                          cgatgtctgtgaagcacaa‑CC‑AGCGACAATCCCGCCAATATGGCGATAAATAACAAATTCCGGT‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAC‑CCAAGTTCTGGCCAGGCAGAACCTACCCCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGACACCGCCGatgatg                                                                                                                                                                                                                     <  2:220053/221‑1 (MQ=255)

                          cGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACAACGACCGAAGCGGTTACTGCAATAACCACGCAGGGCACCGCCgatgtagc                                                                                                                                                                                                                    >  2:637969/1‑235 (MQ=255)

                          cGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACAGACAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGACAAATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTAAACAAGAAATAAAAAAC‑AGGACAGCAGAAATCTAAAGG‑GAATCACGACGACCGAAGCAGTTACTGAAAAAACCACAGAgggaaacaccggcagacg                                                                                                                                                                                                                    >  1:514725/1‑224 (MQ=255)

                           gggagTATTGGGGGTAAACCC‑AACCCAAATACCGCAAATAAGGCGATAAATAACAAATTCCGGG‑A‑AATAACCGGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                                                                                                                                                                                     <  2:112563/236‑1 (MQ=255)

                            agagCATTGCGGCTAAA‑CC‑AACGCCAATACCGACAATAATGCGATAAATAACAAATTACGGG‑A‑CATAACCCGCCAGATAAAAC‑GGCACAGTG‑TT‑GTCCGAGTTT‑ATAGAGGCACAA‑CCACGTTCTGGCCAGGCAGAACCTACACCAGAAATCAAAAAA‑AGGACAGCAGACATCTTAAGT‑GAACCACACCCAACGAAGCGGTATCATCAATACCCAACAAGGGGACCACAGAGGATGaaa                                                                                                                                                                                                                  >  1:1062008/1‑239 (MQ=255)

                            agagCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCCGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGCCGACCAAAAAGGTTACTGCAAAAACCACCGAGGGAACCGCCCATGAGGCaa                                                                                                                                                                                                                  >  1:146755/1‑239 (MQ=255)

                            agagCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGA‑A‑CATAACCTGCCAGATAAACA‑GGCACAGGG‑TT‑GTCCGGGTTT‑ATAGAGCTCAAA‑CCAAGTTCTGGCCAGGCAGAAACTACACCACAAATAAAAAAC‑AGAACAGAGGCAATCTTAAGT‑GAATCACGACGACCGAAGCGTATACGGCAAAAAAAACCGAAGGcccggctctaataaaaa                                                                                                                                                                                                                  >  1:33529/1‑223 (MQ=255)

                              agCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAATAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGCAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAcc                                                                                                                                                                                                                >  2:632225/1‑239 (MQ=255)

                              agCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAACCTTAAGG‑AAATCCCGACGACCGAAGCGGTTACTGCAATAACCACCAAGGGCACCGCCGATGATCAAAcc                                                                                                                                                                                                                >  1:1023108/1‑239 (MQ=255)

                                        aaaaa‑CC‑AACCCAAATCCCGCCAATAATGCGATAAATAAAAAATTCCGGG‑A‑CATAACCTGCCAGAAAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAAAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgcg                                                                                                                                                                                                      <  2:1023108/237‑1 (MQ=255)

                                          ccccaaccccgacaaccccGCTAATAGTGACAAAAATAACCATGTCCGGC‑A‑CATAACCTGCCAGATAAATG‑GGCACGTTG‑TT‑GCCAGGTTGT‑AGAAAGGAAAAC‑ACAAGCTCGTGCCAGGAAGACTCAAC‑CCAAAAAAAAAAAACAAGGCCGGCAGC‑ATTTTAACTGGAACCCGGACGACCGAAGCGGTTCCGGCCATAGCCACCGACGGAACCGCCGATGATGCAACCAATCAGAgcg                                                                                                                                                                                                      <  1:1125159/222‑1 (MQ=255)

                                          aaa‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGCCGACCGAAGCGGTTACTGCAATAACCACCGAGGGCCCCGCCGATGTTGCAACCAATCaaggc                                                                                                                                                                                                       >  2:330974/1‑232 (MQ=255)

                                                   cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACg                                                                                                                                                                                             >  2:482197/1‑239 (MQ=255)

                                                   cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCAAGGGCACCCCCGATGATGCAACCAATCagctggctcttttac                                                                                                                                                                                             >  2:558990/1‑226 (MQ=255)

                                                      aaagcccGCCAAAAATGCAAAAAAAAAAAAATTCCGGG‑A‑AATAACCTGCCAGAAAAACA‑GGCACATTG‑TT‑GTCGGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                                                          <  2:1023414/234‑1 (MQ=255)

                                                       caaccccccAAAAAGGGGAAAAAAAAAAAATGCCGGG‑ATCTAAACCAGCAAGA‑AAACA‑GGCACAGGGTTT‑GCCCGGGTTA‑AGAAGGGAAAAA‑AAAATTCCGGGACAGGAAAGACAAACACAAAAAAAAAAAACC‑AGGACGACAACAATTAGGAGG‑GAACACCGACGCACGAGGCGTTGAAGGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                                                          <  1:1181495/232‑1 (MQ=255)

                                                          aCCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACGCAAAAc                                                                                                                                                                                       >  2:43893/1‑238 (MQ=255)

                                                                 ataatGCTATAAATAA‑AAACTCCGGGAA‑TATAACCCAGCAGATAAA‑A‑GGCCCAAGG‑TTGGTCCGTGTTA‑AAAGAGGAAAAA‑CCAATTTCGGGCCAGGAAGAACCTACACCAAAAATAAAAAAC‑AGGAGACCAGAAATCTAAAGG‑GAACCACGACGACCGAAGTGGTTACTGCAAGACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAg                                                                                                                                                                               <  1:824524/239‑1 (MQ=255)

                                                                     tGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCAGCCAAAACCCTAACAGGGAg                                                                                                                                                                           >  2:258847/1‑239 (MQ=255)

                                                                     agcggataataaAAAAATCCGGGAA‑CAAATCCTGCCAAAAAAACA‑GGCA‑AGTG‑GT‑TTCCGGGTTTAATAGAGAAAAAC‑CAAAGTCCGGGCAAGGGAGAACCTACACCAGAAAAAAAAAAC‑AGGCCAGCACCAATCTTAAGG‑GACCCACGAGGACGGAGGCGTTTACTGCAATAACCACGGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGa                                                                                                                                                                            <  1:888931/232‑1 (MQ=255)

                                                                       ggATAAATAAAAAATCCGGGG‑A‑AATAACCGGACAGAAAAAAA‑GGCACAGTG‑TT‑GGCGGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCGGGCAAGGAAGAACATACACCAGAAATAAAAAAC‑AGGACAGCAGAAAGCTAAAGG‑GAAGCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAg                                                                                                                                                                           <  2:616519/236‑1 (MQ=255)

                                                                          taaataaCAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑AT‑GTCCGGGTTT‑ATAGAGGAAAAA‑CCAAGTTAAGGCCAGGCAGAACCTACCCCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCAAGACAACAGCAGCCGTTACTCAAATAACCACAGAGGGCACCGCCGCTCATGCACACAATAAGAGCACAGGCCACCCAAACACCTAACACGGAGTTgag                                                                                                                                                                      >  1:134604/1‑237 (MQ=255)

                                                                                aCAAATTCCGGG‑A‑AAAAACCTGCCAAATAAACA‑GGCACAGTT‑TT‑GTCCGGTTTT‑ATAGAGTTAAAC‑CCAAGTTCGGGCCAGGCAGAACCTACCCCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGCCCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGa                                                                                                                                                                <  2:914518/239‑1 (MQ=255)

                                                                                    aTTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCAAGGGCACCGCCGAGGAGGCAACCCCTCAGAGCCCTGGCCCCGAACAACACAAAAAGGGAGAGGGCAGGGGATACa                                                                                                                                                            >  1:965325/1‑239 (MQ=255)

                                                                                     aattcggggtatatcacTGTCAGATAAAGAGGGCACAGTG‑TT‑TTCCGGGTTA‑ATAGAGGAAAAC‑ACAAGTTCGGGCCAGGCAGACCCTACACAAGAAATAAAAAAC‑AGGCCAGCAGCAAGTTTAAGG‑TAATCACGACGCCCGAAGCGGTTCCTGCAATAACCCCCGAGGCCTCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGAtt                                                                                                                                                              <  1:67368/223‑1 (MQ=255)

                                                                                                       gCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCCAGGGCACCGCCGGTAATGCACCCAATCAGCGCGCTGGACACGCAACACCATAACCGGGAGTTGGCAGCGGAttacctagagtttgtgggaga                                                                                                                                           >  1:1093498/1‑220 (MQ=255)

                                                                                                          aaaTAAACG‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGTTAAAC‑CCAAGTTCTGCCCAGGCAAAACCGACACCAGAAATAAAAACC‑AGGACAGCAGCAATCTTAAGG‑GAATCACGACGACCGAAGCGTTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAACCCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGcaac                                                                                                                                       <  2:1117377/237‑1 (MQ=255)

                                                                                                           aaaaaaccgggCACGGTG‑TT‑GTCCGGGTTT‑AAAGAGGAAAAC‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACGGCAGCAATCTTAAGG‑GAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGcaac                                                                                                                                       <  2:429288/230‑1 (MQ=255)

                                                                                                            aTAAACA‑GGCACAGTT‑TT‑GTCCGGGTTT‑ATAGAGGTAAAC‑CCAAGTTCGGGCCAGGCAAAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGcaacaa                                                                                                                                     <  2:612085/239‑1 (MQ=255)

                                                                                                            aTAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGCAGTTGGCAGCGACTTCAC‑TTAAGTTTTGTGGAGcaacaa                                                                                                                                     >  2:1170031/1‑239 (MQ=255)

                                                                                                                     gCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCATCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACggga                                                                                                                             >  2:997057/1‑237 (MQ=255)

                                                                                                                       acaGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATAAGGGCTCTGGCCACGCAAAACCCTAACAGCGAGTTGGCAGAGGATTCAC‑TTCAGTTTTTGGGGGAACCAAAAACgggaat                                                                                                                           >  1:87325/1‑235 (MQ=255)

                                                                                                                          ttg‑tt‑gtCCGGGTTG‑AAAAGGGTAAAA‑CCAACTTCTGGCCAGGCAAAACCAACCCCAAAAAAAAAAAAC‑AGGACAGTAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAg                                                                                                                       <  2:1131199/238‑1 (MQ=255)

                                                                                                                           tg‑tt‑gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGAATT‑GAgg                                                                                                                      >  2:90867/1‑238 (MQ=255)

                                                                                                                             ttt‑gtCCGGGTTA‑AAAGGGGAAAAA‑CCAAGTTCGGGCCAGGAAGACCCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCATAATT‑GAATCACGACGCCCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGt                                                                                                                      <  2:506249/237‑1 (MQ=255)

                                                                                                                              tt‑gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAAACGGAATT‑GAGTg                                                                                                                     >  2:630485/1‑238 (MQ=255)

                                                                                                                                 gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGGTTCAC‑TTAAGTTTTGTGGAGCAACAAAGCCGGTATT‑GAGTGAc                                                                                                                   >  2:565166/1‑238 (MQ=255)

                                                                                                                                 gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAAACGGAATT‑GAGTGACt                                                                                                                  >  2:524795/1‑239 (MQ=255)

                                                                                                                                 gtCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAAACGGAATT‑GAGGGACt                                                                                                                  >  2:996128/1‑239 (MQ=255)

                                                                                                                                  tCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGG‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACACAAAACCCTAACAGGGAGTTGGGAGCGGATTCAC‑TTAAGTTTTGTGGAACAACAAAGACGGTAAT‑GAGTGaacc                                                                                                                 >  1:716570/1‑236 (MQ=255)

                                                                                                                                     cgttttttaaggtgaaaaa‑CAAATTACTGCCCAGGAAAACCCTACCCCAGAAAAGAAAAAA‑GAGACAGCGAAAATCTGAAGT‑GAATCACGACGCCCGAGGCGGTAACTGAAGTAGCCACAGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCGAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCaa                                                                                                               <  1:630485/224‑1 (MQ=255)

                                                                                                                                      cggttttaaaagGTAAAACCCAAGTTCTGGCCGGGCGAAACCTACACCAAAAAAAAAAAAC‑GGTACAGCAGCAAGCTTAAGT‑GAACCCCCACGACCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCaa                                                                                                               <  2:134604/229‑1 (MQ=255)

                                                                                                                                       gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCCGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGAAGACCGAAGCGGTTACTGTAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCACAAGCCTGGCCAAGCAATACCCCAACAGGGGGTTGGTAGCGGCTTCAC‑TTAAATTTTGTCGAGCAACAAAGACGGTATT‑GAggagccaaacaa                                                                                                            >  1:704444/1‑228 (MQ=255)

                                                                                                                                       gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCCATCTTAAGT‑GAATCACGACGACCGAAGCGGTTATTGCAATAACCACCGAGGGACCCGCCGACGGTGCCACCAATCAGAGCGCTGGCCAAGCAAAACCCCAACAGGGAGTTGGCGGCGGA‑TCACATTAAGTTTTTGGGAGAAAAAAAAACGGGAtgggaggaccaaaaag                                                                                                             >  1:683109/1‑224 (MQ=255)

                                                                                                                                       gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGGGCAACAAAGACGGAATT‑GGGTGACCCAACaa                                                                                                            >  2:1024795/1‑238 (MQ=255)

                                                                                                                                       gTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCCAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCTAAACCCTAACCGGCGGTTGGCAGCGGATTCCC‑TTAAGTTTTGTGGAGCACCAAAGAGGGTATT‑GAGTGCCTCAAAAg                                                                                                            >  2:1227995/1‑239 (MQ=255)

                                                                                                                                            ataGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCC‑TTAAGTTTTGTGGAACAAAAAAAACGATAttaaagaacacaaaagaaac                                                                                                         >  1:23906/1‑221 (MQ=255)

                                                                                                                                             taGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCg                                                                                                       >  2:796930/1‑239 (MQ=255)

                                                                                                                                             taGAGGAAAAA‑CAAAGTTCGGGCAAGGCAGAACCTAACCCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGCCCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCg                                                                                                       <  2:665844/239‑1 (MQ=255)

                                                                                                                                                  gTAAAA‑CCAAGTTCTGGCCCGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATCATGCAACCAATCCGAGCGCTGGCCACGCCAAACCCTAACAGGGAGGTGGCAGCAGCATCAC‑TTAAACTTTGTGGAGAAACAACAACAGCATT‑GAGTGTATCAACAAAAACGGAAAt                                                                                                  >  1:450832/1‑239 (MQ=255)

                                                                                                                                                  gTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GACTCACGACGACCGAAGCGGTTACTGCAATAACAACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCCCGCAAAACCCTAACAGGGCGTTGGCAGCGGATTCCA‑TTAAGTTTTGTGGAGCAACAAAGACGGAATT‑GAGTGACTCAAACGTACCGGAAAt                                                                                                  >  2:488848/1‑239 (MQ=255)

                                                                                                                                                  gTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACACTAACAGGGAGTTGGCAGCCGATTCAC‑TTAAGTTTTGTGGCGCAACAAAAGAGGTATT‑GAGGGAATCAAAATAACAGGaaaa                                                                                                  >  1:441094/1‑238 (MQ=255)

                                                                                                                                                   gaaaa‑CCAAGTTCTGGCCAGGCAGAACATACACAAGTAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATTGCGACGACCGAAGCGGATACTGCAATAATCACCGAGGGCACCGCCGATGATGGAACAAATCAGAGGGCTGGCCACTCAAAACCCGCACCTGGGTCGGGCAGCGGAGTCAC‑TTAAGGTTTGTGAAGCCACAAAAAAGGTATT‑TAGCGACTTCACATAAACGGaaaaa                                                                                                 >  1:269760/2‑237 (MQ=255)

                                                                                                                                                    aaac‑CCAAGTTCTGGCCAGGCAGAACCTACACCAAAAAAAAAAAAC‑AGGCCAGCAGCAATTTTAAGT‑GAACCACGACGGCCGAAGCGGTTACGGAAATAACCCCCGAGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATaa                                                                                                <  2:995924/239‑1 (MQ=255)

                                                                                                                                                          cAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGCCAGCAGCAATCTTAAGT‑GAACCACGACGACCGAAGCGGTTACTGAAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAAcggcg                                                                                           <  2:822374/239‑1 (MQ=255)

                                                                                                                                                            aGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACCGGGAGTTGGCAGCGGCGTCAC‑TTAAGTTTTTTGGAGCAAAAAAGACGGAATT‑GAGTGACTCAACCTTACCGGAAATAACGGCGgt                                                                                         >  2:714248/1‑239 (MQ=255)

                                                                                                                                                             gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCC‑TTCAGTTTTATGGAGCAACAAAAACGGTATT‑GAGCGACTCAACAGTACCGGCAATAAaggcggag                                                                                        >  2:1249206/1‑237 (MQ=255)

                                                                                                                                                              gtcgggacAGGAAAAACATACACCAGAAAAAAAAAAC‑AGGACAGCAGAAATCTTAAGG‑AAACCACGACGCCCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGgtgt                                                                                       <  2:1093498/232‑1 (MQ=255)

                                                                                                                                                                  ggCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAACAATCAGAGCGCTGGCCACGCCAAACCCTAACAGGGAGTTAGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGTTATT‑GAGTGACTACACAAGAACGGAAAACAcggcgggatggag                                                                                   >  1:812509/1‑232 (MQ=255)

                                                                                                                                                                   gCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACTCAAAACCCTAACAGGGAGTTGGCAGCGTATTCAC‑TTAAGTTTTGTGGCGCAAAAAAGACGGTATTAGAG‑GACGCAACACAACCGGAAATAAcgtcgggggtggac                                                                                  >  1:1019353/1‑231 (MQ=255)

                                                                                                                                                                   gCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGgttgccgt                                                                                   >  2:190449/1‑232 (MQ=255)

                                                                                                                                                                     cgggCAGACCCTAACCCAAAAAAAAAAAAC‑AGGACCGCAGCAACTTTAAGT‑AAACCACGACGACCGAAGCGGTTACTGAAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGcc                                                                                <  2:490370/237‑1 (MQ=255)

                                                                                                                                                                     cggcgaacaCCTACCACAAAAAAAAAAAGC‑AAGACAGCAGAACTTATAAGT‑GAACCCGCACGACCGAAGGGTTTACGGAAACACCCACCGAGGCACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGcc                                                                                <  1:489521/231‑1 (MQ=255)

                                                                                                                                                                        gCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGCGGGCACCGCCGATGATGCAACCAATCTGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGGGCAACAAAGACGGTATT‑GAGTGACTCAACCGTACCGGAAATAACGCCGgtgcgtgctgccaa                                                                             >  2:1137047/1‑228 (MQ=255)

                                                                                                                                                                        gCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACt                                                                                                                  >  2:954687/1‑202 (MQ=255)

                                                                                                                                                                               ttaacacaaaaaGAAACAAC‑AGGACATCAGCAATCTTAAGT‑GAACCAGGCCGACCGAAGCGGTTACTGAAAAAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  2:110459/230‑1 (MQ=255)

                                                                                                                                                                               tcaccccAGAAAAAAAAAAC‑AGGACAGCAGCAACCTTAAGT‑AAATCACGCTGACCGAAGCGGTTACTGCAATAACCACGGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  2:81597/234‑1 (MQ=255)

                                                                                                                                                                               cTACACCAAAAAAAAAAAAC‑AGGACAGCAGCAATCTAAAGT‑GAACCCCGCAAACCGAAGCGGTTACTGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  2:683109/239‑1 (MQ=255)

                                                                                                                                                                               accccccAAAAAAAAAATAC‑AGAACACCGTCAATCTGAAGC‑GACCCCCGACACCAGAAGCGTGTACTCCAATCCCCCCCGAGGCGACCGCGGATGGGGCACCCAATCAGAGCGCTGGCCACGCAAAACACTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  1:671802/234‑1 (MQ=255)

                                                                                                                                                                                tACACCAGAAAAAAAAAAC‑AGGACAGCAGAAATCTTAAGT‑GAATCACGACGCCCGAAGCGGTTCCTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  2:904119/238‑1 (MQ=255)

                                                                                                                                                                                 ggctgggagatgtgtaaaagaaaCAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  2:943887/216‑1 (MQ=255)

                                                                                                                                                                                  cacCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGGGGCGTAGCCAAAAAATaaaaaa                                                                   >  2:480191/1‑236 (MQ=255)

                                                                                                                                                                                          aTAAAAAAC‑AGGACAGCAACAATCTTAAGT‑GAACCACGACGCCCGAGGCGGTTACTGCAATAACCGCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGt                                                           <  2:1062008/239‑1 (MQ=255)

                                                                                                                                                                                           aaaaaaaC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACCGAAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  2:10672/226‑1 (MQ=255)

                                                                                                                                                                                                      gACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGGGACTCAACATTACCGGAAATAAGGGCGGGGGCGGTGTCGAATAATAaacaacaaaaagacagtctactaa                                                >  1:10672/1‑221 (MQ=255)

                                                                                                                                                                                                          acacaaaTCTTAAGT‑AACACACCACCAACGAAGCTGTAACCCAAACAACCCCCGAGTACACCGCCGATGATGCAAACAATCAGAGCGCTGGCCTCGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACTGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAAt                                            <  1:1247864/234‑1 (MQ=255)

                                                                                                                                                                                                           cagcaATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGGGTCGTAGCCCAAAAATAAACCACCTACTTTTGGGACCAAGGCaaac                                           >  2:873067/1‑237 (MQ=255)

                                                                                                                                                                                                            agcaATCGTAGGT‑GAATCACGACGACAGAGGCGGTTACTGAAAGAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAAt                                            <  2:700261/237‑1 (MQ=255)

                                                                                                                                                                                                             aaaaTCTTAAGG‑GAACCCCGACGACCGAAGCGTTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGa                                         <  2:988493/237‑1 (MQ=255)

                                                                                                                                                                                                               aaTCTTAAGT‑GAATCCGGTCGGCCGAAGGGGTTACCGCAAAAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGa                                         <  2:129559/237‑1 (MQ=255)

                                                                                                                                                                                                                         gaaaccccgccgacCGAAGCGGTTACTGCAATCACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGAAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  2:1268784/229‑1 (MQ=255)

                                                                                                                                                                                                                          gAATCACGGCGACCGAAGCGGTAACGGCAATACCCCCGGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  2:492906/238‑1 (MQ=255)

                                                                                                                                                                                                                          aaaacagcacgacCGAAGCGGTTCCTGCAATACCCACCGAGGTCACCGCCGATGAGGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  2:450832/230‑1 (MQ=255)

                                                                                                                                                                                                                            aTCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTACGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGAGTCGGAGCCAAAAAAAAAAACACCCAAAGAAAAGACCAAGGGAAAGGaaaaaaaaaaaaaa                            >  1:988493/1‑228 (MQ=255)

                                                                                                                                                                                                                              cctgacgccCGAAGCGGTTCCTGCAATACCCACGGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGaa                         <  2:681883/236‑1 (MQ=255)

                                                                                                                                                                                                                                   cgacCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAg                                                                                                                                   >  2:684123/1‑129 (MQ=255)

                                                                                                                                                                                                                                          aggtgTTACCGCAATAACCACCGGGGTCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTa             <  2:294803/235‑1 (MQ=255)

                                                                                                                                                                                                                                          aggggTTCCTGAAATAACCCCCGGGGCCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTa             <  2:110604/236‑1 (MQ=255)

                                                                                                                                                                                                                                             ggTAACTGAAATACCCACCGAGGGACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTat            <  2:1158309/237‑1 (MQ=255)

                                                                                                                                                                                                                                                     cAATCACCCCCGAGGGCCCCGCCGATGATGCAACCAAGTCGGGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGTGAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGAc  <  1:996128/239‑1 (MQ=255)

                                                                                                                                                                                                                                                     cAATACCCCCCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGAc  <  2:965325/239‑1 (MQ=255)

GGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACA‑GGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGTAAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAC‑AGGACAGCAGCAATCTTAAGT‑GAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAC‑TTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGAC  >  NC_000913/4241787‑4242259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`