Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,300,841 A→G 11.2% L64P (CTC→CCC)  mdtP ← outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,300,8410AG11.2% 369.8 / 4.8 148L64P (CTC→CCC) mdtPouter membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base A (53/72);  new base G (12/7);  total (67/81)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

GTATAGTACGGCCCGTCCATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGG  >  NC_000913/4300604‑4301077
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            
ttataGTCCGGCCCTCCCCACACCAGTGCAGGCGCATGCAGTCAAGAAGGGATTAAAAAGCCGGTCGCCGGACCTCGTTGGCGGTTGTGCCATCCTAACGCTGCGGCCTGTAATTGTGAACCGGCCTCTAAAAAATCGGCCTGTGCCTGCGCTTTTTCTTCCCGCAGTTTCGCTGCGGCCAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAg                                                                                                                                                                                                                                             <  1:881754/238‑1 (MQ=255)
gTATAGTACGGCCCGTCCATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAg                                                                                                                                                                                                                                             <  2:923326/239‑1 (MQ=255)
atataGTCCGGCCCGTCCATACCCAGTGCTGGTGCATCCATTGAATAAGGGCTTAAAAAGTCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAg                                                                                                                                                                                                                                             <  2:264843/238‑1 (MQ=255)
      tACGGCCCGTCCATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGGGCATCCCTAACGCAGCGACCCGTAATTGTGAACCGGCCTCTAACAAAACGGCCCGGGACTCCGCTTTTTCTTCCCCCAGTTTCGCCTCGGCCGGGGTGGGCAAACCCACTAACCGCCGTTTGATCCACCCCTCCAGCTGCGGGGGATTGAggtggt                                                                                                                                                                                                                                        >  1:572514/1‑236 (MQ=255)
         ggCCCGTCCATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTTGCTTCGGCGAGGGTGTGTGAACCACTTAACGTCCGTTGGATCAGCGCCACCAGCTACGGGCACTTGAGTTGTTTct                                                                                                                                                                                                                                     >  2:46060/1‑237 (MQ=255)
           cccGTCCATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGTGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTtcac                                                                                                                                                                                                                                   >  2:1160357/1‑238 (MQ=255)
           cccGTCCATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGGGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCTTTGGGTTGTTTtcacc                                                                                                                                                                                                                                  >  2:319825/1‑239 (MQ=255)
           cccGTCCATACCCAGTGCTGGCGCATCCATTGAATAAGAGCTTAAAAAGCCGTTCGCCGGGACCCGTTGGCCGGTGAGCATCCCTTACCTTGCGCGCTGTAATTTTGCACAGGCGTCTAGACAATCGTCCGCGGGTCGCGCTTTTTCTTCCCCTAGATTCCCTTCCGCGGAGGGGTGTGGACCAATTAGCCTCCGTTGGATCACGCGCACCACCTAGCGGACATTGAGGTGTTTccacc                                                                                                                                                                                                                                  >  1:32599/1‑239 (MQ=255)
                ccATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTTCGGGTCATTGAGTTGTTTCCCCCCCTg                                                                                                                                                                                                                              >  2:816128/1‑238 (MQ=255)
                ccATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGGCATTGAGTTGTTTCCACCACTg                                                                                                                                                                                                                              >  2:1090326/1‑238 (MQ=255)
                           tGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGATCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTTGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGGGAACCACTTAGCGTCCGTTGGGTCAGCCCCTTCCGGCCAGGGGCACTGGTGTGTTTCCACCACCGCCCCTGccga                                                                                                                                                                                                                    >  1:143504/1‑235 (MQ=255)
                           tGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTTCCGCAGGTTTGTTTCGGCGAGGGGGGGTGGACCACCTAGCGTCCGTTGGATCAGCGCATCCACCTGCGGGCCATGTGGTTGGTTccacacatgggcccgcgggc                                                                                                                                                                                                                   >  1:201672/1‑223 (MQ=255)
                           tGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGCACCACTTAGCGTCCGGTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCCCTGCGCCTGCGGcc                                                                                                                                                                                                                   >  2:176249/1‑239 (MQ=255)
                           tGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGGGTGTGAACCACTTAGCGTCCGTTGGGTCAGCGCCTCCAGCTGCGGGGCATTGAGTTGTTTCCCCCACTGCGCCTGCGGc                                                                                                                                                                                                                    >  2:1083012/1‑238 (MQ=255)
                            gCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCCCTTCGGCGAGGGGGGGTGAACCACTTAGCGTCCCTTGGATCAGCGCAACCCGCCGCGGGGCCTTTGGTTGTTTCCACCCCAGCGCCTGCGGCCa                                                                                                                                                                                                                  >  1:291749/1‑239 (MQ=255)
                               ggCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGc                                                                                                                                                                                                                <  2:63572/238‑1 (MQ=255)
                                gcgcATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGGGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCCCTGCGCCTTCGGCCAGCCg                                                                                                                                                                                                              >  2:1145078/1‑239 (MQ=255)
                                   cctCCTTTGAATAAGGGCTTAAAAAGCCTGTCGCCGGGCGCGGTTGGCGGTTGAGCATCCCTAACGCTGGGACCTGTAAGTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGATCCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAg                                                                                                                                                                                                           <  1:1100566/237‑1 (MQ=255)
                                    aTCCATTGAATAAGGGCTTAAAAAGCCGTTCGGCGAAGCACGTTGGCGGTTGAGCATCCCTAACGCTGGGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAg                                                                                                                                                                                                           <  2:293843/238‑1 (MQ=255)
                                     tCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGGCATTGAgttgtt                                                                                                                                                                                                                                        >  2:1084201/1‑208 (MQ=255)
                                     tCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGGGTGTGAACCACTTAGCGTCCGTTGGATCAACGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGa                                                                                                                                                                                                            >  2:1078263/1‑236 (MQ=255)
                                     tCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTAGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCGGCGGGGGATTGGGTTGTTTCCCCCACTGAGCCCTGGGCCAGACGGAGcc                                                                                                                                                                                                         >  2:967975/1‑238 (MQ=255)
                                        ttttaaaaggggTTTAAAAGTCCGTGCGCCGTGACCCGTTGGGGGTTGAGCTTCCCTACCGCTGCGACCTGTAATTGTTAACCGGCATCTAACAAATCGGGCTGGGGTTTCGCTGTTTCTTCCCGCAGTTTCGTTTCGGCGAGGGTGTGTGAACCACTTAGCGTTCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGc                                                                                                                                                                                                      <  1:1225923/230‑1 (MQ=255)
                                        cgtGAATAAGGCGTTAAATAGCCGTTCCTCGGAGCACTTTGGCGGTTGAGCATCCCGAACGCTGCGATCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTGCGGCGAGGGTGTGGGAACCGCTTAGCGTCCGTCGGAGCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGc                                                                                                                                                                                                      <  1:483703/237‑1 (MQ=255)
                                        aTTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGc                                                                                                                                                                                                      <  2:1148581/239‑1 (MQ=255)
                                        aTTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGc                                                                                                                                                                                                      <  2:215461/239‑1 (MQ=255)
                                        aTTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGGGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGc                                                                                                                                                                                                      <  2:1286943/239‑1 (MQ=255)
                                           taaTAAGGGCTTAAAAGGCCGTTCGCCGGGACCCGGTGGCGTTTGTGCATCCCTACCGCTGCGACCTGTAATTGTGAACCTGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACAGCTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAg                                                                                                                                                                                                   <  1:1251543/238‑1 (MQ=255)
                                                 gggCTTAAAAGCCGGTTCGCGGTGACATCGTGGCGGTTGAGCATCCCTCACGCGGCGGCCTGTAATTGTGAACCGGCATCTAACAACTCGGCCTGGGACGTCGCTTTTTCTTCCCGCATTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGGCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGaa                                                                                                                                                                                              <  1:25898/238‑1 (MQ=255)
                                                         aaaGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCgg                                                                                                                                                                                     <  2:73453/239‑1 (MQ=255)
                                                         aaaGCCGTTCCCCGGGATGGTTTGGCGGTTGAGCACCCCTAACGCTGCGACCTGTAATTGTGAACCGGGATCTAACAAACCGGGCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTTTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCgg                                                                                                                                                                                     <  1:671771/239‑1 (MQ=255)
                                                          aaaccGTTCGCCGAGACACGTGGGCGTTTGAGCATCCCTAACGCTGGGGCCTGTAATTGGGAACCGGCATCTAACAAATCGGCCTTGGACTGCGCTTTTTCTTCCCGCAGGTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGGTCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGc                                                                                                                                                                                    <  1:1046090/236‑1 (MQ=255)
                                                             ccGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGGGTTGTTTCCCCCCCTGCGCCTGCGGCCAGCCGGGGCTGGCAAGATGAATATCGTCGGCCAg                                                                                                                                                                                 >  2:636213/1‑239 (MQ=255)
                                                             ccGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAAAATCGTCGGCCAg                                                                                                                                                                                 >  2:671771/1‑239 (MQ=255)
                                                              cGTTCGCCGAGACACGTTGGGGGTTGGGCATCCCTAACGCTGGGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGt                                                                                                                                                                                <  2:865746/239‑1 (MQ=255)
                                                               gTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAACGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCCCCTGCGGGCAGCCGGGGCTGGCAAGATGAATATCGTCGGCCCGtt                                                                                                                                                                               >  2:1249103/1‑239 (MQ=255)
                                                                   gCCGAGACGTGTTGGCGGTTGAGCATCCCCAACTCGGCGTCCTGTAAGTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGTTTTTTCTTCCCGCAGTTTCGTTTCGGCGGGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCACCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGAt                                                                                                                                                                           <  1:774467/239‑1 (MQ=255)
                                                                   ccctggccccGTTTGCGGTTCATCAGCCCTAAGGCTGCGACCTGTAATTGTGACCCGGCAGCCAACAAATCGGCCTGGGGCTGTCCTTTTTCTTCCCGCAGGTTCGCTTAGGCGAGGGTGTGTGAACCACGTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGAt                                                                                                                                                                           <  1:726405/230‑1 (MQ=255)
                                                                         ccccGTTGGCCGTTGAACATCCCTACCGCTGCGCCCTGGTATTGTGAACCGGCCTCTAACAAACCGGCCTGGGACTGCGGTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGtt                                                                                                                                                                      <  1:451151/235‑1 (MQ=255)
                                                                                           ctCCCGAACGCTTCGACTTGTAATTGGCAACCGGCCTCTTACAAAGCGGCCTGGGAAGGCGCGTTTTCTTCCCGCAGTTTCGCTTCGGCGATGGTGTGTGAACCACTTAGCGTCCGGTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTtgat                                                                                                                                                    <  1:1066010/237‑1 (MQ=255)
                                                                                           aTCCCTAACGCTGCGCCCTGTAATTGGGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTtgat                                                                                                                                                    <  2:147431/238‑1 (MQ=255)
                                                                                           aTCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAAGCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTtgatg                                                                                                                                                   <  2:143504/239‑1 (MQ=255)
                                                                                            tCCCTAACCCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTtgatga                                                                                                                                                  <  2:1005778/239‑1 (MQ=255)
                                                                                            ccctttcctgtgggtccTTTATTTGTGAACCGGTAGCTCACAAATTGGGCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGTGTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCTCATCCAGCGGCGGGTCATTGAGTTGTTTCCACCACTGCTCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTtgatga                                                                                                                                                  <  1:157020/224‑1 (MQ=255)
                                                                                                tAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTg                                                                                                                                              <  2:18347/239‑1 (MQ=255)
                                                                                                       gcgcCCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGGACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGTCCAGCCGGCGGTGGCGAGGTGAATAACTTCGGCCAGTTTGATTTTTTCCGGTTTGAGcgtgtaaagggagctaggatc                                                                                                                                       >  1:627933/1‑219 (MQ=255)
                                                                                                       gCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCCCTGCGCCTCCGGCCAGCCGGGGCTGGCAAGGTGATATTCGTCGGCCGATTTTTTTTGTTCCGGGTTGGGcgtttttatgggggcaaaac                                                                                                                                        >  1:187930/1‑219 (MQ=255)
                                                                                                        cGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCCTCCAGCTGCGGGGCATTTAGTTGTTTCCACCCCTGCGCCCGCGTCCCGCCGGGGCTGGCAAGATGAATTCCTTCGGCCAGTTTGTTTTTCTCCGTTTTGGGCTGTTGCTGGGGGGCCGaaaac                                                                                                                                      >  1:803061/1‑236 (MQ=255)
                                                                                                        cGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCCGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGaac                                                                                                                                        >  2:196112/1‑236 (MQ=255)
                                                                                                           cgtttAATTGTTAAGCTGCATCTATCCTATCGGCCTGGCTCTTCGCGTTTGTTTCCCGCAGTCTCGTTCGGGCGGTGGTGTGTGATCCTCCTCGTGTCCGTGGTATCAGCTCTTCCATGTGCCGGTCATGATTTTGTGTCCTCCACTGCGCCTGAGGCCAGCCGGAGTGGGCAATATGACTATCGTCGGCCAGTTTGATGTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTa                                                                                                                                   <  1:744609/235‑1 (MQ=255)
                                                                                                                      gAACCGGCATCTAACAAATCGGCCTGGGACTTCGCTTTTTCTTCCCGCCGTTTCGCGTTGGCGAGGGTGTGTGAACCACTTAGCGGCCGTTGGATCAGCGCATCCAGCTGCGGGTCCTTAAGTTGGTTCCACCACTGCGTCTGCGGCCAGCCGGAGCTGGCAAGTTGAATACTGTCGGCCCACTTGATTTGGTCCGGTGTGAGCTGTTGATGGTGTGCCGCATCCTTACGTAACAggga                                                                                                                        >  1:1001621/1‑238 (MQ=255)
                                                                                                                        aCCGTCATCTAACGAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCATTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGcac                                                                                                                      <  2:366879/239‑1 (MQ=255)
                                                                                                                              agcTAACAAATCGGCCTGGGACTTCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCg                                                                                                                 <  2:462047/236‑1 (MQ=255)
                                                                                                                                       tccgtctttggctttgcgttttttttTCCGCAGTTTCGGTTCCGTGGGGGTGTTGTAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGGGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGcg                                                                                                       <  1:337906/221‑1 (MQ=255)
                                                                                                                                       aTCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAACCGGAAATCAGc                                                                                                        >  2:246282/1‑238 (MQ=255)
                                                                                                                                         cGGCCTGGGACTGCGTTTGTTCCTCCCGGAGTTTCGCTTGGGCGAGGGTGTGGGGCCCCCTGGGCGTCGGTTGGATCAGCGGATCCAGCTGGGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAAGATCGTCGGCCAGTGTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGcgtc                                                                                                     <  1:46060/239‑1 (MQ=255)
                                                                                                                                           gCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAg                                                                                                                                                            >  2:455985/1‑182 (MQ=255)
                                                                                                                                             tttgtgtggcgtTTTTTCCTCCCACTTTTGCGTTTCGGCGAGGGTGTGTGAACCAGTTGGCGTCCGTGGGAGCAGCGCCTCCAGCTGCGGGTCATGGAGTTGTTTCCAGCACTGCGCCTGCGGCCGGCCTGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGCTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCGTACGTACCAGGGCACAGCCGGAAATCAGCGTCGtg                                                                                                  <  1:735496/230‑1 (MQ=255)
                                                                                                                                             cggggACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGtg                                                                                                  <  2:330809/236‑1 (MQ=255)
                                                                                                                                             cTGGGACTGCGCTTTTTCTTCCCGGAGTTTCGCTTCGGCGGGGGTGTGTGAACCCCTTAGCGTCCGTTGGATCAGCGCAGCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGtg                                                                                                  <  1:1180071/238‑1 (MQ=255)
                                                                                                                                                      cgcTTTTTCTTCCCGCATTTTCGCTTCGGCGGGGGTGTGTGAACCCCTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGaa                                                                                        <  2:625221/239‑1 (MQ=255)
                                                                                                                                                      cgcTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGaa                                                                                        >  2:96964/1‑239 (MQ=255)
                                                                                                                                                      cgcTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCGGCCGAGaa                                                                                        >  2:319118/1‑239 (MQ=255)
                                                                                                                                                      cgcTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGCCGTGCTGCCGGGaa                                                                                        >  2:26790/1‑239 (MQ=255)
                                                                                                                                                      cgcTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGAGGAATACCGTCGGCCAGGTGGTTTTGTTCCGGGTTGCGCTGTTGATGAGGAGCAAAATCCTTACGTACCAGGGCACAGCCGGAAATCatggcctggtggccgctaa                                                                                        >  1:176050/1‑221 (MQ=255)
                                                                                                                                                        cTTTTTCTTCCCGGAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAAtg                                                                                      <  2:707725/239‑1 (MQ=255)
                                                                                                                                                         tttttCTTCCCGTAGTTTCGCTTCGGGGAGGGTTTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAAtg                                                                                      <  2:840344/238‑1 (MQ=255)
                                                                                                                                                              cttcCCGCATTTTCGCTTCGGCGAGGGTGTGTGAACCCCTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGcac                                                                                <  2:665634/239‑1 (MQ=255)
                                                                                                                                                              cttcCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGcac                                                                                <  2:803061/239‑1 (MQ=255)
                                                                                                                                                                            gattCGGCGAGGGTTGGTGAACCCCTTAGCGTCCGTTGGACTAGGCCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGa                                                                  <  2:40782/237‑1 (MQ=255)
                                                                                                                                                                                cGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGTGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCTTGCTGCCGAGaa                                                                                        >  2:766563/1‑213 (MQ=255)
                                                                                                                                                                                  gggggtggtttgggtaccctttAGCGTCGGGTGGATCAGCGCATCCAGCTGCTGGTCATTGGGTTTTTTCCACCCCCGCGCCGTCGGCCAGCCGGAGCTGGCAAGATGAATAGCGTGGGCCATGTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGtt                                                             <  1:1083012/219‑1 (MQ=255)
                                                                                                                                                                                  gggggggttggggAACCCATTTGCGTCCGTGGGAACGGCGCGTCCAGGCGCGGGTCGTTGGGATGTTTCCGCCACTGCGCCTGCGGCCAGGCGGAGCTTGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGtt                                                             <  1:1005608/226‑1 (MQ=255)
                                                                                                                                                                                   gaccggggtttgAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGtt                                                             <  2:1104418/227‑1 (MQ=255)
                                                                                                                                                                                    ttttttaaggcaccgcccTTTGCTTCCTTTGGATCAGCGCATCCCGCTGCGGGTCATTGAGTTGTATCCCCCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGa                                                           <  1:527761/222‑1 (MQ=255)
                                                                                                                                                                                     gggggggttttaacacattAGCGTCCGGTGGATCAGGCCATCCGGTGGCGGGTAATTGGGTTGTTTCCCCCACTGCGCCTGCGGGCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGa                                                           <  1:26790/221‑1 (MQ=255)
                                                                                                                                                                                       ggTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAAcagca                                                                         <  2:1246483/221‑1 (MQ=255)
                                                                                                                                                                                            gtccggtgttgataagagccagggATCAGCGCATCCAGGTGCGGGTACTGGAGTTGTTTCCACCACTGGGCCGGCGGACAGCCGGAGCTGGGAAGATGAATATCGGCGGCCAGTTTGATTTGTGCCGGGTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCGGCACAACAGCAGACGTGAAAGTTGACGATTGAt                                                   <  1:533573/217‑1 (MQ=255)
                                                                                                                                                                                                   cACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGc                                                                                                                                                                                                      <  2:953702/85‑1 (MQ=255)
                                                                                                                                                                                                   cACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGc                                                                                                                                                                                                      >  1:953702/1‑85 (MQ=255)
                                                                                                                                                                                                    aCTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGGGAATGCTCCACAAAAGCAGACGGGCAAGtttggaattgatccgttc                                             >  1:1104418/1‑221 (MQ=255)
                                                                                                                                                                                                      ttAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTg                                          >  2:755653/1‑238 (MQ=255)
                                                                                                                                                                                                      gggccggccGTTGGGTCAGCCCATCCAGGTGCGGGTCATTGAGTTGTGTCCACCACGGCGCCTGGGGCCAGCCGGGGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGGTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTgg                                         <  1:816128/232‑1 (MQ=255)
                                                                                                                                                                                                           gTCTGTTGTATCCGCGCTTTCGGCTGGGGGTCATTGGTTTTTTTCCCCCCCTGCGCCTGCGGCCAGCCGGGTCTGGCAAGTTGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCt                                    <  1:65043/239‑1 (MQ=255)
                                                                                                                                                                                                              cGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCa                                                 >  2:527761/1‑223 (MQ=255)
                                                                                                                                                                                                               gTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGGGAATGCTGCACACCAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTggt                                 >  2:531697/1‑238 (MQ=255)
                                                                                                                                                                                                                tttgATCAGCGCATCCAGGTGGGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATTAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGc                               <  2:39839/236‑1 (MQ=255)
                                                                                                                                                                                                                          cgcATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACACCAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGt                      >  2:1253249/1‑239 (MQ=255)
                                                                                                                                                                                                                          cgcATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGt                      >  2:1281987/1‑239 (MQ=255)
                                                                                                                                                                                                                          cgcATCCAGCTGCGGGTCATTGAGCTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGa                                                                                                                                                                                                  >  2:627010/1‑67 (MQ=255)
                                                                                                                                                                                                                             atcgggatgtgggTCATTGAGTTGTTTCCACCCCTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGCATATCGTCGGCCAGGTTGATGTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTggc                   <  2:1113184/229‑1 (MQ=255)
                                                                                                                                                                                                                              tCCAGCTGCGGGTCATTTGGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTggcg                  <  2:330188/239‑1 (MQ=255)
                                                                                                                                                                                                                              tCCAGCTGCGGGTCATTGAGTTGTTTCCCCCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGGCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTggcg                  <  2:201672/239‑1 (MQ=255)
                                                                                                                                                                                                                              tCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCCCAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTgg                                         >  2:533573/1‑216 (MQ=255)
                                                                                                                                                                                                                              tCCAGCTGCGGGTCATTGAGGTGTTGCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAGGATGAATATAGTCGGCCAGTTTGGTTTGTTCCGGTTTGAGCTGTTGATGAGGGGCAGAATCCTTACGTACCAGCGCACAGACGGAGATCAGCGTCGTGCCGCCGAGCATGCGGCACAACAGCAGCCGTGCAACTGGCCCATGGACAATAGCGTGGCGCATTGTGCCTTTTCCGTgagg                  >  1:293426/1‑236 (MQ=255)
                                                                                                                                                                                                                               ccAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCCTTGCCTGGCTCCTTGTGCCTGTTCCGTggcgg                 >  2:469151/1‑239 (MQ=255)
                                                                                                                                                                                                                                     gCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGtt            <  2:1001621/238‑1 (MQ=255)
                                                                                                                                                                                                                                          tCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGc       <  2:291749/238‑1 (MQ=255)
                                                                                                                                                                                                                                            aTTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTAc                                                                                                                              >  1:882177/1‑117 (MQ=255)
                                                                                                                                                                                                                                            aTTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGGGAAAGTTGACGATTGATCATTGCGGGGCTCCTTGTGCCTGTTCCGTGGCGGGGGGTAATGcccg    >  2:342628/1‑237 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            
GTATAGTACGGCCCGTCCATACCCAGTGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGG  >  NC_000913/4300604‑4301077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: