| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 458,189 | A→G | 14.8% | D255G (GAT→GGT) | clpX → | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 458,189 | 0 | A | G | 14.8% | 194.1 / 4.3 | 77 | D255G (GAT→GGT) | clpX | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease |
| Reads supporting (aligned to +/- strand): ref base A (9/53); new base G (10/2); total (22/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGA > NC_000913/457951‑458425 | gggtataaggttctggTCTACAGCGATTAAATCGGCAACATTTCTC‑GTAAGT‑CAGTCAACCGGTTCATTGCCCGAGACATTTCCGGTGAAGGCGTTCAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAATCATCCGCAGCAGGAATTCTTGCAGGTTGATACCACTAAGATCCTGTTTATTTATGGCGGTGCGTTTGCCGGTCT‑GGa < 2:181137/224‑1 (MQ=255) cacaGGCTGGTATTGTCTTCATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGAt < 2:994742/239‑1 (MQ=255) taaaagggaaaggtctcaatccgtGAAATCGACAAGATTTCTC‑GTAAGTACAG‑CAACCCGTCCATTACCCGGGGCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATaa < 1:1015161/216‑1 (MQ=255) gggtttttttGTCTACATCGATGAAACCGACCAGATTTTTG‑GCAAGC‑CAGAACACCCGTCCATGACCCGAGGCGTTGCCGGTGAAGGCGTACGGCCGGCCATGGTGGAGCGGATCGAAGGTACGGTAGCTGCTGTTCCACAGCAAGGTGGGCGTAAACATCCGGAGCAGGAATTCTTGCAGGTTCATAACTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAg < 1:995581/231‑1 (MQ=255) agagacagttGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAg < 2:606801/231‑1 (MQ=255) gTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAg < 2:189820/237‑1 (MQ=255) gtctcaaTCGATGAGATCGACATAGTTTCGC‑GAAAGT‑CAGACAACCCGTCCATTTCCCTGGGCGTTTCCGGTGAAGGCGTCCCGCAGGCACTGTTGAAACTGAGCGAAGGTACGGTCGCTGCTGTTCCACCGCAAGGGGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCa < 1:1289911/233‑1 (MQ=255) gTCTAAATCGATGAAAGCGACAAGGTTTCGC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGGTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCa < 2:786145/239‑1 (MQ=255) cTACATCGATGAAAGCGACAAGA‑TTCTCGGTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCAc < 2:1244916/238‑1 (MQ=255) cTAAATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCAcc < 2:1123031/239‑1 (MQ=255) tACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTTGCGGTGCGGTTTCCGGTCT‑GGTTAAAAGGATTTTTCAccc > 2:1235085/1‑238 (MQ=255) aTCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCCGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGGGGGGTTGGCGGGCT‑GGATaaaaagaatttccaacggtg > 1:382926/1‑222 (MQ=255) aTCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGCTTTTCTGTCT‑GTCTAAAGTGAGTTTCCAcccgct > 2:970279/1‑235 (MQ=255) tGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCTGGGCT‑TGGTAAAATGATTTcccctgtctctttt > 2:1015161/1‑227 (MQ=255) tGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGGTAAAGAGATTTCCAACCctgtgggaa > 2:1139855/1‑234 (MQ=255) aGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCT‑GGGTAAAAGGATTTTCCCCCGTGGAG‑AAAACGGCCCCGGCC‑TTTGTTTTggg > 2:537281/1‑238 (MQ=255) caacacaCCCGTCCATTACCCGAGGCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGgcgc < 2:1002558/232‑1 (MQ=255) gACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCG‑GGTTAAATGGATTTCTCCCCGGGTGG‑CAAACGCACCCGGCC‑TTGGgttttcacagcg > 2:80187/1‑231 (MQ=255) cAACCCGTCCATTGCCCGATCCGGTTCCGGTGAAGGCGTACCGCAGGCACGGTTGAACCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTAT‑GGATAAAGTGATTTCCCTCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACgg < 2:714740/239‑1 (MQ=255) cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATaaaa > 2:517912/1‑178 (MQ=255) cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAg < 1:517912/179‑1 (MQ=255) aTTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGaa < 2:949766/239‑1 (MQ=255) gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGGTTTCCGGGCT‑GGATAAAGTGAGTTTCACCCAGGTAGAAAACGGGCCCCGCCC‑TTGGTTTTTGCG‑GACGGTcaaacgcaatgctcgc > 2:1070363/1‑227 (MQ=255) agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTTCCGGGCT‑TGGTAAAGTGGTTTTCCCCCCTGGAG‑GAACCGGCCCCCGCA‑TTGGGTTTGGTGCGACGGTTAAACGCAAGTcccgca > 2:781949/1‑235 (MQ=255) agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTTCGGTCT‑GGGTAAAAGGATTTCCCCCCGTGTAG‑AAACCCGCTCCGGCC‑TTGGTTTTGGCGCGACGGTCAAAGCGAAGTCCGACa > 2:203872/1‑239 (MQ=255) tGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTTTGGCGGTTGGCTTGTCGGGCT‑CGGTCAAGCGGTTTTCCCCCCTGTTG‑GAAACGGCCCCTGCACTTGCTTTGGTCGCGCCGGAAAAAGCaaaagcgacaaacaaacgcgaa > 1:899939/1‑220 (MQ=255) cTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTCTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGt < 2:996759/239‑1 (MQ=255) gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTTCGTTTGCCGGACT‑GGGTAAAGTGCATTTCCACCGTGTAG‑AAACCGGCTCCGGCATTTGGTTTTGG‑GGGCCGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGGCGCTGGCGCCGGTTg > 2:201529/1‑239 (MQ=255) gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGTCT‑TGATAAAGGGATTTTCCACCGTGTAG‑AAAACGGCTCCGGCA‑TTGGTTTTTGCGCGGCGGGAAAAGAGAAGGCCGGCAAAACAAACGAAGGCGAGCTGCTGGGCCAGGGTg > 2:686838/1‑239 (MQ=255) gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTTGCGGGCT‑GGATAAAATGATTTTCCCCCGCGTAG‑AAAACAGGCCCGGCA‑TTTGGTTTGGGGCGGCGGTGAAAGCGAAGAGCGCCAAAAAAAGCGAAGGGGGGCTGCTggggggggggg > 1:1196546/1‑230 (MQ=255) gTTGAAACTGAACGAAGGTAGGTGAGTGGCTGTTCCACCGTAAGGTGGGCGTAAACAACCCGAGAAGGAATTCTTGGAGGTCGATACCTGTAAGATCCTGTTTATTTGGGGCGGTGCGGTTGCCGGTCT‑GGATAAAGTGAGTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCTCAGGTTg < 1:201529/239‑1 (MQ=255) tttgatgggctggggggTTCGGTGGCGTCTGTTCCCCCGAAAGGTGGGCCTAAAACTCCGCAGCAGGAATTCTTGCAGGTTGATTCCTCTTAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑TGATAAAGTGATTTCCCCCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa < 1:760013/224‑1 (MQ=255) ttGAAACTGATCGAGGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAGCATCCGCAGCAGGAATGCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa < 2:197806/239‑1 (MQ=255) ttGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGGCT‑GGGTAAAATGATTTTCCACCGTGTCG‑AAAACCGCCCCGGCA‑TTGGGTTTGGTGCGCCGGGAAAAGAGAAGACCGGCAAAGCAAGCGAAGGCGGCCTGCTGTGGCCGGTTGa > 2:1074885/1‑239 (MQ=255) ttGAAACTGACTGAAGGTGCGTGGTCTGCTGTGCCACCGCACGGGTGGCGTTAACATCCGCATCAGGAATTCGTGAGGGTTGATACCGTTAAGCTCCTGTTGATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGGGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa < 1:781949/239‑1 (MQ=255) ccTGATCGAAGGGACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCgg < 2:170455/238‑1 (MQ=255) tCGAAGGTACGGTAGCTGCGGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt < 2:537719/239‑1 (MQ=255) cGAAGGTTCGGTAGGTGCTGTCCCCCCGCAAGGTTGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt < 2:511365/238‑1 (MQ=255) tgttcggtggttgtttttCCCCCGCATGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTTTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt < 1:537281/223‑1 (MQ=255) tACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg < 2:922143/214‑1 (MQ=255) cGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGACCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGATAAA‑TGATTTCTCCCCGTGTAG‑AAAACGTCTCCGGCG‑TTGGGTTTGGAGCGCGGGCAAAATAGAAAGCCGGCAAAGAAAGCGCAAGCGGGCGGGTGGGGCAGAGTGAAAAGGaaaaaatgaacaaa > 1:27905/1‑227 (MQ=255) gtcgctcttgTTCCCCCGCAATTGTGGCGTAAAATTCCGTAGCAGGAATTCTTGACGGTTGATACCTCTAAGGTCCTGTTGTTTTGTGGCGGTGCGTTTGCCGGTCG‑GGATAAAGTGATTTCCCACCGGTTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGGGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 1:644447/231‑1 (MQ=255) tAGCTGCTGTTCCACCGGAAGGTGGGCGTAAACCTCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 2:1094244/238‑1 (MQ=255) tAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACCTCCGCAGCAGGAATTCTTGCAGGTTTATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 2:1205243/238‑1 (MQ=255) ggctgcgGTTCCCCCTGAAGGGGGGGGTAAACATCCGAAGCAGGAATTCTGTCAGGTTGATACCTCGAAGATCCTGTTTAGTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGttt < 1:834255/237‑1 (MQ=255) gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGGTAAAGTg > 2:32436/1‑111 (MQ=255) gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTg < 1:32436/111‑1 (MQ=255) gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCt < 2:1196546/239‑1 (MQ=255) ccACCGGAATGTGGGCTGAACCATCCGCAGCAGGAAATCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc < 2:870587/239‑1 (MQ=255) ccACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc < 2:568557/239‑1 (MQ=255) cccctgaaGGTGGGTATAAACCTCCGGAGCAGTAATTCTTGCAGTTTCGTCCCTCTAAGAGCCTGTTTGTTTGTGGCGGCGCGTTTGGCGGGCC‑GGATAAAGTGAGTGCCCACCGTGTAG‑AAACCGGCTTCTGCC‑TTTGTTTGGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGACGAGCTGCTGGCGCAGGTTGAACCGGAATATCTGATCAAGTTTGGTCTTATCCCt < 1:313096/233‑1 (MQ=255) ccccGTAAGGTGGGCTTAACCATCCGGAGCAGGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTCTATTTGTGGCGGTGCGGTGTCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt < 1:260982/237‑1 (MQ=255) gccGCAAGCGGGGCGTAAACATCCGGAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCAGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTg < 2:177259/238‑1 (MQ=255) ccGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 2:539900/224‑1 (MQ=255) gggAATTTTTGCAGGTTGATACCTCTAAGATCATGTTTAATTGGTGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCa < 2:958429/238‑1 (MQ=255) agGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCg < 2:382926/237‑1 (MQ=255) aaTTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATATAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc < 2:983329/238‑1 (MQ=255) aaTTCTTGAAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCCCCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGATAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCa < 2:673463/236‑1 (MQ=255) ttGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg < 2:1208849/159‑1 (MQ=255) ggTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCT‑GGATAAAAGGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGGTTTTGCGCGACGGTAAAAGCGAAGTCCGCCAAAACAAGCGAAGGCGAGCGGCTGGCGCAGGGTGCACCGGGAGCACCGAGACAGCTTGGTACTCTCCCTGAGTTGTTTTGGCTTTTTCCGGTTGtgcggacagtgtttgaaa > 1:1121084/1‑222 (MQ=255) ttGATACCTCTAAGGTCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtgaatgaa < 2:823613/236‑1 (MQ=255) ctctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTc < 2:168042/179‑1 (MQ=255) ctctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑gaag < 2:857284/239‑1 (MQ=255) tctAAGGTCCTGTTTATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑gaaga < 2:974074/239‑1 (MQ=255) aTCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGa < 2:942758/38‑1 (MQ=255) aTCATGTTTATTGGGTGCGGTGCGTTTGCCGGTCT‑GGATAAAGGTATTACCGACCGTGTAG‑AAACCGGCTACGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGctct < 2:435958/239‑1 (MQ=255) cTGTTTATTTGTGGCGGTGCGTTTTCCGGTCT‑GGGTAAAGTGATTTTCCACCGTGTAG‑AAAACGGCTCCGGCA‑TTGGGTTTGGCGCGACGGGAAAAACGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGGCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTGTTGGCAACGTTGAATTAACTGAGC‑GAGGAAGCTCTGAt > 2:326541/1‑239 (MQ=255) tGTTTATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGAtt < 2:1105375/239‑1 (MQ=255) tttATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCa < 2:910734/239‑1 (MQ=255) tttATTTGGGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCa < 2:1156287/239‑1 (MQ=255) tgGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGCTTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGGCGGACGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATATGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCa < 1:203872/239‑1 (MQ=255) ggggggTGCGTTTGGCGGTCG‑GGAGAAAGGGAGTTCCCACGGTGTAG‑AAACCGGCTCAGGCA‑TTGGGTTTGGCCCGGGCGTAAAAGCGAAGTCCGAAAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTTTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCa < 1:1074885/235‑1 (MQ=255) ggggTGCTTTTTCTGGGCT‑GAGTAAAGGTATTTCACACCGTGTAG‑AATCCGGCTTGGGCA‑TTGGTGTCGGCGCGACGGTAAAAGCGAAGTCCGTCAAAGCAAGCGAAGGTGAGATGCTGTCGCAGGTTGCACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGGTGAATGAACTGAGC‑GAAGAAGCGCTGATTCGGACCATCaaa < 1:210137/237‑1 (MQ=255) ggggtgttttgcggtttttgATAAAGGGATTGCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TGGGTTTTGGCGCGCATGTAAAAGCGAAGGCCGACAAAGCAAGCGAAGGCGAGCTTCTGGCTCAGGTTGAACCTGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCaaa < 1:686838/219‑1 (MQ=255) ggTCT‑GGGTAAAGTGATTACCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGc < 2:260512/239‑1 (MQ=255) tCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAACGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAGCAAGTTTGGTCTTATCCCTTAGTTTATTGGTCTACTTCCGGTTGTTGCCAAGTTGCAGCAACTGAGC‑GAAGAAGCTCTGATTCACATCCTAAAAGAGCCGCAAACCGcca > 1:364237/1‑238 (MQ=255) cT‑GGAAAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGccc < 2:1093492/238‑1 (MQ=255) t‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGCAGGCGAGCTGCTGGCGCAGGGTGAACCGGAAGATCTGATCAAGTTTGGGCTAATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAGTGAAAT‑AGCGGAATAAGCCCGGGTTCAGAACCCAAAACAGACAAAAAACGcccaa > 1:685890/1‑237 (MQ=255) ggATAAAGTGGTTTCCCTCCGGGTAG‑AAAGCGGGTCCGGCA‑TTGGTTTTGGCGCGTCGGTAAAAGCGAAGTGCGAAAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGACGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACTCCCTGa < 2:428283/239‑1 (MQ=255) | GCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGT‑CAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCT‑GGATAAAGTGATTTCCCACCGTGTAG‑AAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGA > NC_000913/457951‑458425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |