| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 766,690 | T→C | 31.6% | L236P (CTC→CCC) | mngA → | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 766,690 | 0 | T | C | 31.6% | 11.3 / 2.5 | 16 | L236P (CTC→CCC) | mngA | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component |
| Reads supporting (aligned to +/- strand): ref base T (8/2); new base C (2/2); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
GGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAA‑C‑GGCGGATCAACTCAATAGTG‑CAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGA > NC_000913/766454‑766922 | ggCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGGTTAA‑C‑GGCGTGTCCACTCa > 2:753638/1‑239 (MQ=255) cgCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGGCGCAAACTCGGCGTAAATGTTTCCGTTGAAAAACAAGGCGGTAACGGCCTTGAAGGGCGGTTAA‑C‑GGCGGGTCAACCCacaaaggcc > 2:1082174/1‑231 (MQ=255) cgCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGCCGCAAACTCGGCGTAAATTTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAA‑C‑GGCGGATCAACTCCAAAGtac > 2:1212609/1‑236 (MQ=255) ctctCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTTAAGGGCGTTTAA‑C‑GGCGGATCCAATCCAAAGTG‑CAACccccctcatttt > 2:684075/1‑231 (MQ=255) cAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAAAAAGGCGCTAACGGCCTTGAAGGGGTTTTAA‑C‑GACGGAGGACACCAATAAGG‑CAAACGCCTGGCATTTTTCGGCTGAAATCGccc > 2:541489/1‑238 (MQ=255) tGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAA‑C‑GGCGGATCAACTCaa < 1:1046382/137‑1 (MQ=255) ctaatagGGCTGTGAAATATAAGGAAAACGCCGGCCAAAACCTGGGAAAAATTTTTTAGGTTAAAAAACAGGGC‑ATGAGGGGATTGAAGGGGTTTTAAGC‑GGGGAATCACCACAATAGGC‑AAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCcaa < 2:1230660/236‑1 (MQ=255) tatGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAA‑C‑GGCGGATCAACTCAATAGTG‑CAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGGTTTAAATGCATTCCCCCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCCTGCAGAAGCGTTGATCcaacaa > 2:359102/1‑238 (MQ=255) cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAA‑C‑GGCGGATCAACTCAATAGTG‑CAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCCCGCTTTCAGTGCCTGTTGCCGAGCCGCTTCGCCATGCAGAAGCGTTGTTccccgtctctttt > 2:847627/1‑228 (MQ=255) cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAA‑C‑GGCGGATCAACTCAATAGTG‑CAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGGTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTTCCGAGCCGATTCGCCATGCCGAAGCGTTGATCCAACAAacgcct > 2:388590/1‑237 (MQ=255) acgcgggagcaaaaCTGGGCATAATTTTTCAGGTTAAAAAACAGGGC‑CTAAACGATATGGAGGGGTTTTAA‑CGGGCGGACCAACCAAATAGTG‑CAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGAt < 2:116302/228‑1 (MQ=255) gCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAA‑C‑GGCGGATCAACTCAATAGTG‑CAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGGATTTCCCCGCTTTCAGTGCCCGTTTCCGGGCCGGTTCGCCCTGCAGAAACGTTGATCCAACAAacg > 2:813219/1‑216 (MQ=255) aaaccggggataaattttttACGTAAAAAAACAGGGCG‑TAACGGGATTGAAGGGGTTTTAA‑A‑GGGGGATAAACCAAAAGGGGACACCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGcgta < 2:988212/222‑1 (MQ=255) tgttacggggaggATCCACAAAATTGTG‑CAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAgg < 2:397861/227‑1 (MQ=255) cggATCAACTCAATAGTG‑CAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGGGTCAAAAAGGGGCTGAAACacaggctcttatac > 2:983702/1‑226 (MQ=255) acAACTAAATGGGG‑CACCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGa < 2:1179199/237‑1 (MQ=255) | GGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAA‑C‑GGCGGATCAACTCAATAGTG‑CAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGA > NC_000913/766454‑766922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |