Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	811,721	T→G	30.6%	V67G (GTG→GGG)	bioC →	malonyl‑ACP O‑methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	811,721	0	T	G	30.6%	41.6 / 4.5	41	V67G (GTG→GGG)	bioC	malonyl‑ACP O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (11/15);  new base G (11/4);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCAT‑GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGT  >  NC_000913/811485‑811957                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               gAAATGCAGGATACCGACCGTTTGCTGGAGGTGCTGCAT‑GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGAAGCCGCACACTAG‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                              <  2:768973/239‑1 (MQ=255)   aaTGCAGGATATCGACCGTCTGCGGGAGGTGCTGCAT‑GGAAACGTTTAAAAAACAAGCAATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                              <  2:761844/237‑1 (MQ=255)        ctgatttccgtctggtgctgGGGGTGC‑GCATGGGCAAAGGTAAATCAACAAGCCATGGGAGCGGAATTTGGCTGGGCAGCCGCACAATAT‑GAGCAACATGC‑GAA‑CTAACTACGCAA‑GAGGTCGTACGACTTACGGGCAATGCTTCCACGGCTTAAATGCACCCAC‑GTACTGGAAGCGGGTTGTGGCGCTGGC‑TGG‑ATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCtt                                                                                                                                                                                                                                       <  1:773376/224‑1 (MQ=255)                  ccGGCTGGTGGGGGTGCGTTAT‑GGCAATGGTTAATAAACAAGCCATTGCGGCGGCATTTGGTCGGGAGCCCCCACACTAT‑GAGCACCACGC‑GATCCG‑ACAGCGCCA‑GAGTGCCGACGCCTGCCGGGAAATGCTCCCACAGCGTAAATCCCCCCAG‑GTACGGGACGCGGGTTGTGGCCCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTcg                                                                                                                                                                                                                             <  1:288697/239‑1 (MQ=255)                    gTCTGCTGGAGGTGCTGCAT‑GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCAAGCGCAGGTGACGGCCTTAGATCTCTcgc                                                                                                                                                                                                                            >  2:846684/1‑238 (MQ=255)                      ctgctgGAGGTGCTGCAT‑GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGGCACCGCCAGGGGACGGccctgagttttccgccc                                                                                                                                                                                                                          >  2:1277770/1‑223 (MQ=255)                               gTGCTGCAT‑GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGGGCAGGGGACGGCCTTAGATACCTCGCCGCCCA‑TGCTTg                                                                                                                                                                                                               >  2:476596/1‑239 (MQ=255)                                   tgcAT‑GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCGAGGGACGGCCTTTGACCCCCCgccgcccaagcgttttcc                                                                                                                                                                                                            >  2:990297/1‑227 (MQ=255)                                   tgcAT‑GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCCCCCA‑TGCTTGTTCa                                                                                                                                                                                                           >  2:281307/1‑239 (MQ=255)                                       gtttataagaaacagaaaCAAGCCTTTGCAGGGTCTTTTGGGCGGGGAGCCGCACATTAT‑GAGAAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATCCACCCAC‑GTACTGGACGGGGGTTGGGGCCCTGGC‑TGG‑ATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAgg                                                                                                                                                                                                         <  1:429000/223‑1 (MQ=255)                                           cAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGTAACGTCACGCGCAGGTGACGGCCTTTGACCTCTTGCCGCCCA‑TGCTTGTTCAgggagg                                                                                                                                                                                                     >  2:1104900/1‑234 (MQ=255)                                                gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGGACGTACCGCGCCGGGGACGGCCTTAGCACTCTCGCCGCCAC‑TGCtttgtgcgggccccccgaa                                                                                                                                                                                               >  2:233293/1‑222 (MQ=255)                                                          aaGCCATTGCGCCGGCATTTGGTCGGG‑AGGCCCACACCATAGAGC‑ACATAGCGATACT‑AC‑GCGCCAGGAGTGCTGTCGCCTTACGGGCAATGCTTCCAGAGGGTAAATCCCCCCGC‑GTACGGGACGCGGGTGGGGGCCCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCa                                                                                                                                                                                    <  1:281307/239‑1 (MQ=255)                                                           aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCCCAGGTGACGGGCTTCGGTCTCCTGCCGCCCA‑TGCTTGTTCTGGCACCCCAGCAG‑GATGCCGcc                                                                                                                                                                                    >  2:549596/1‑237 (MQ=255)                                                           aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGGCACCCGCACGTGCCGGCCCCAGATCTCTCGCCGCCAC‑TACTTGTTCAGGCACCCCAGCAG‑GAGGCCCCAg                                                                                                                                                                                   >  2:235858/1‑239 (MQ=255)                                                           aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGCCACGCGCAGGGGCAGGCCTTAGATCTCTCGCCGCCCA‑TGCTTTGTCAGGCACCCCAGAAG‑GAGGCCGCa                                                                                                                                                                                    >  2:813224/1‑238 (MQ=255)                                                           aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGCCAAGCGCAGGGGACGGCCCTAGATCTCTCCCCGCCCA‑TGCTTGTTCCAGCACCCCAGAAG‑GAGGccccgg                                                                                                                                                                                   >  2:1200995/1‑235 (MQ=255)                                                           aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGCCGGGAACGTCACGCGCAGGTGACGGCCTTAGAACTCTCGCCGCCCA‑TGCTTTTTCAGGGCCGCCAACAG‑GAAGcccccg                                                                                                                                                                                   >  2:622756/1‑235 (MQ=255)                                                            gCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCCA‑TGCTTGTTCAGGCACGCCAGCAG‑GAAGCCGCAg                                                                                                                                                                                   >  2:523382/1‑238 (MQ=255)                                                                ttGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGCCACGCGCCAGTGACGGCCCTAGATCTCTCGGCGCCAAGACCTTGTT‑AGGCACACCCCAAG‑GAGGcccgcaccaca                                                                                                                                                                              >  2:537421/1‑230 (MQ=255)                                                                 tGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGAGCCCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAgctgtcccttatacaca                                                                                                                                                                              >  2:429000/1‑223 (MQ=255)                                                                                    gCAGCCGCACTCTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCg                                                                                                                                                           <  2:431058/238‑1 (MQ=255)                                                                                                  t‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCCA‑TGCTTGTTCAGGCACGCCCGAAG‑GATGCCGCAGACCATTATCTGGCGGGGGATATCGAATCCCCGcccgta                                                                                                                                             >  2:1262337/1‑234 (MQ=255)                                                                                                      gCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCCGGTGTCGGCCCTAGGTTGCTCGCCGCCCC‑TGCTTGTTCAAGCACGCCAGAAGAGAGCCCGC‑GACCACTATCCGGAGGGAGataatcaaacccccccgcgagcga                                                                                                                                         >  1:392632/1‑218 (MQ=255)                                                                                                           aTGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCCTAGATCTCTCGCCGCCCA‑TGCTTGTTCAGGCACGCCCGACG‑GGAGCCGCCGGCCATTAt                                                                                                                                                                           >  2:707170/1‑200 (MQ=255)                                                                                                               aGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCCCCGGGGACGGCCTTAGATCTCTCGCCGCCCA‑TGCTTGTTCAGGCCCCCCAGAAG‑GATGCCGCCGACCCATATCTGGGGGGGGGTATCCAATCCCTGCCGTTTGCGCCCTCGACGt                                                                                                                                >  2:253007/1‑239 (MQ=255)                                                                                                                     ttcaacgccccagtggccTGACCCCTTACGGGCAATGCTTCCCAAGGGTAAATACCCCCAC‑GTCGGGGCCGCGGGTTGGGGCCCGGGC‑TGG‑ATGGCCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGtt                                                                                                                               <  2:392632/220‑1 (MQ=255)                                                                                                                                   tgtctgcccccTTACGGGAAATTCCTCCCAGGCGTAAATCCCCCCAA‑GGACTGGACGGGGGTTGGGGCCCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAg                                                                                                                <  2:824740/229‑1 (MQ=255)                                                                                                                                      gtttccccTTTCTGGGAATGGTTCCACATGGTAAAT‑CACCCCCTGGTCTGGGCGGGGGGTGGGGCCCCGGT‑TGGCAGGCGCCCCCCAGGGGGGGAGCGTCACCGGCAGGGGCCGGCTTTAGATCCCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCaa                                                                                                             <  1:476596/233‑1 (MQ=255)                                                                                                                                                                              ccAC‑GTGCGGGACGGGGGTTGGGGACCTGGC‑TGA‑ATGAGCCGCCACGGGGGGGAACGTCCCGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTcc                                                                    <  2:1258898/239‑1 (MQ=255)                                                                                                                                                                                    tACTGGCCGGGGTTTGGGGACCGGGC‑TGG‑ATGAGCCGCCACTGGGGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAg                                                               <  2:1134148/239‑1 (MQ=255)                                                                                                                                                                                    gtccgggacggggTTGGGGACCCGGCGGGG‑AGGGCCCGCCACTGGCGGGAACGTCCCGGGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCGGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGa                                                                <  2:167086/229‑1 (MQ=255)                                                                                                                                                                                                    gggggctggggtgg‑ATGGGCCGCCACTGGGGGGAACGTCAGGGGAAGGGGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCGGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGgtggtg                                                <  2:94361/228‑1 (MQ=255)                                                                                                                                                                                                                   aTGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCCA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGGCTGCGACGTTCGATCTTGCATGGAGCAAACTCGCAGTGCAGTGGTGCGGGAATTTATTCACGGCACTCCGCGGGCTGTCTCCGGGGGGGGGCCCCAAAGGCgg                                  >  2:744845/1‑238 (MQ=255)                                                                                                                                                                                                                         cgcccggggggtgagcgagcggcgcAGGTGCCGGTCTTACATCTCTCGCGGCAAA‑TGCTTGGTCAGGCACGCCAGCAT‑GATGCCGCAGACCATTATCGGGCGGGGGATAGCGACTCCCTGCCGTGAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTcgc                            <  1:233996/218‑1 (MQ=255)                                                                                                                                                                                                                          gcccttggggggacctcaccgcgTAGGGGCCGGCTTTAAACCCCTCGCCGCCAT‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGCCATTTATCTGGGGGGATATATCGATTCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTcgc                            <  2:813647/219‑1 (MQ=255)                                                                                                                                                                                                                               gggggggAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTAc                      <  2:109167/235‑1 (MQ=255)                                                                                                                                                                                                                                           cACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCGGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGGGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCCGGGGGTGCGCCCCAAAGGCGGGGGCGCGTTTACCACGCggggtca          >  1:389784/1‑235 (MQ=255)                                                                                                                                                                                                                                            ccgggaaGGGGACGGCCTTAGATCTCGCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAg         <  2:770663/233‑1 (MQ=255)                                                                                                                                                                                                                                                  gggTGACGGTTTTAGCCCTCCCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATc    <  2:207721/237‑1 (MQ=255)                                                                                                                                                                                                                                                    gTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGGAATTTATCCACGGCCCCCCGCGGGCTGTATCGGGGGGGGCCCCCCCAAAGGGCGGTCACGTTTAcacccccgtggcggggatcgg  >  1:1095032/1‑218 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               GAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCAT‑GGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT‑GAGCAACATGCAGAT‑CT‑ACAGCGCCA‑GAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAC‑GTACTGGACGCGGGTTGTGGACCTGGC‑TGG‑ATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAA‑TGCTTGTTCAGGCACGCCAGAAG‑GATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGT  >  NC_000913/811485‑811957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑