Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 921,011 T→G 27.7% V222G (GTG→GGG)  macB → macrolide ABC transporter peremase/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913921,0110TG27.7% 67.9 / 2.0 44V222G (GTG→GGG) macBmacrolide ABC transporter peremase/ATPase
Reads supporting (aligned to +/- strand):  ref base T (21/9);  new base G (6/8);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGC  >  NC_000913/920774‑921247
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
cacaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGTGGATGGCGATCCTGCATCACCTGCGCGATCGTGGGCATACGGTGCTTATCGTCACCCCAGAACTGCAGGTCGAGGCTCAGGCCGAGCAGGGGCTCGCACTTCACGACGGCGAAATtgtg                                                                                                                                                                                                                                                >  1:362874/1‑239 (MQ=255)
cacaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGGGATCGAAATTCGCGCCGGCGAAATtgt                                                                                                                                                                                                                                                 >  2:607634/1‑238 (MQ=255)
cacaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCCCGGGCCGAGCGGGGGATCGAAATTCGCGACGGCGAAATtgt                                                                                                                                                                                                                                                 >  2:62312/1‑238 (MQ=255)
 acaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGGGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACAGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATtgtg                                                                                                                                                                                                                                                <  2:1200543/238‑1 (MQ=255)
  aaGCTTTCGGGTGGTCAGAAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTgcg                                                                                                                                                                                                                                              <  2:1139529/238‑1 (MQ=255)
    tgtttCGGGTGGTCAACAACAGGGCGCCAGCATCGGGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCa                                                                                                                                                                                                                                            <  2:688731/237‑1 (MQ=255)
       ttCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGAGAAGAGGTGATGGCGATCCTGCATCAGATGCGCGCTCGTGGCCATCCGGTGCTTATCGTACCCCACGATCCGCAGGCCGCTGCCGCGGCCAAGCGGGGGATCGAAAATCGCGACGGCGAAACTGGACGCaac                                                                                                                                                                                                                                          >  1:67794/1‑237 (MQ=255)
             tggtCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTccc                                                                                                                                                                                                                                    >  2:214155/1‑238 (MQ=255)
               gtCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGGGGGCATACGGTGATTATCGTCACCCACGGTCCGCCGGTCGCGGCTCATGCCGGGCGGGTGTTAGGAATTCGGCGCGGCACAATTTGGCGAAACCATCCCGc                                                                                                                                                                                                                                  >  1:168627/1‑238 (MQ=255)
                tCAGCAACAGCGGGTCAGCATCGGGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCa                                                                                                                                                                                                                                            <  2:126044/227‑1 (MQ=255)
                          cgcgTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCCCGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATCCGCGACGGCGAAATTCGGCGCAATCCCCCCGCCCTTGAAAAAgg                                                                                                                                                                                                                      >  1:1022366/1‑238 (MQ=255)
                          cgcgTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCCCAGGTCGCTGCTCCGGCCGAGCGGGTGATCGCAATTCGCGACGGCGAAATTGGGCGCAATCATCCGGCCCTTGAAAAAGt                                                                                                                                                                                                                      >  2:1261661/1‑239 (MQ=255)
                                                   tgaTCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGACGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGCCGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGGCGGaa                                                                                                                                                                                               >  2:1011042/1‑236 (MQ=255)
                                                      aCGGTGGTCAGGTAATTCTTGCCGATGCACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATAGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAAGGGTACTGGCGGGACGGAACCggtt                                                                                                                                                                                         >  2:523551/1‑239 (MQ=255)
                                                      aCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCTTTGAAAAAGGGAATGTTACTGGCGGGCGGGAACCtttt                                                                                                                                                                                         >  2:586288/1‑239 (MQ=255)
                                                      aCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGGGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGGGCGTGTGATCCAAATTCGCGCCGGCGAAATTTGGCCCAACCCTCCCCCCATTGTAAAAGGGAAGGTTACTggggggcggggacaaggg                                                                                                                                                                                         >  1:429988/1‑227 (MQ=255)
                                                      aCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGCGATGGCGATCCTGCATCAGCTGCGCGCTCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGATCAGGCAGAGGGGGGGATCGAAATTGGCGACGGCGAAATTCGGCGCAAATCACCCGCCATTGAAAAAGGGAAGGGTACTggggggggcagcaaggtg                                                                                                                                                                                         >  1:1141286/1‑227 (MQ=255)
                                                      aCGGTGGGCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGTCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCCGCTCCGCGATAGTGGGCATACGGGGATTATCGTCACCTAGCATCCGCAGGTCGCTCTTATGGCGGAGCGGCTGATCGCAATTGGCGAGTGCGACATTTTGCGAAATCCTCCAGCCCTGGCACAAGAGCATGGTACTgcatggcggcaccattgt                                                                                                                                                                                         >  1:313495/1‑222 (MQ=255)
                                                       cGGTGGTCAGGGAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCCTACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCCCGACGGCGACATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGTACTGAAccactt                                                                                                                                                                                         >  1:283119/1‑234 (MQ=255)
                                                         gggggcgggTAATTCTGCGCGATGAACCACCGGGCGCACTGGACAGCCATTCGGGCGAAGAGGTTATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCCCCCACGATCCGCAGGTCGCTGCTCAGGCCGGGCGGGTGATCGAAATTCGGGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCtgttg                                                                                                                                                                                        <  2:1022366/230‑1 (MQ=255)
                                                          tggtCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGGGAATGTTACTGGCGGGGCGGGACCCGTTGTcc                                                                                                                                                                                     >  2:1173306/1‑238 (MQ=255)
                                                            gtCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCTCGACGGCGAAATGGTGCGCAATCCGCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAAAACGTTGTCAac                                                                                                                                                                                   >  2:600347/1‑239 (MQ=255)
                                                                    aTTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGGAACcgttgtgacacaggggcc                                                                                                                                                                           >  2:200494/1‑227 (MQ=255)
                                                                                                     aGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACCCGGTGGCTGGCTGGGGGCAGTTTGTTATCGGTTTTAAcca                                                                                                                                         >  2:1258321/1‑237 (MQ=255)
                                                                                                          ttCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACAAGGCGTTCGGCTGGCGGCAGTTTTTccagggatttaaaagggcgc                                                                                                                                    >  2:570280/1‑220 (MQ=255)
                                                                                                            cTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCCCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCCGTTTTCAACACGGGTCCTGTCCTGCGGCATGTTTGCCGCGGGTTTAACGCGgggcgg                                                                                                                                  >  2:810333/1‑237 (MQ=255)
                                                                                                                         gATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGGACCTGTTGTCAACACGGGGTCTGGCTGGCGGCAGATTGTCACCGGGTTTAACGAGGCGGTggacatggactggc                                                                                                                     >  2:1056989/1‑226 (MQ=255)
                                                                                                                                 tCCTGCATCAGTTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCCGGTCGCTGCTCAGGCCGAGCGGGGGATCGAAATTCGCGACGTCGAAATTGTGTGCAATCCTCCCGCCCTTGAAAAAGTGAATGTTACTGGGGGGGAGGGAACCGGTGCTCACAAGGCGTCTTGTTGCCTGCAGTTTTTCCGCGGGTTTTACAAGgcgccgcccagagcgcgggggggggcg                                                                                                             >  1:49101/1‑216 (MQ=255)
                                                                                                                                 tCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGCTTGTCAGCGGTTTTAACGGGGGCATGACGAAGGCCTggcggggcgtg                                                                                                             >  2:401348/1‑234 (MQ=255)
                                                                                                                                 tCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAAAAAGGGGTCTGGCTGGTGGCAGTTTTTCAGCGGTTTTAACGAGGCGCGGACGCTGGCATGGCGGgggcgg                                                                                                             >  2:367255/1‑237 (MQ=255)
                                                                                                                                            cTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGGACCCGATTGCACACCGGGTTTTGGCTGGGGGCCGATTGGCAGCGGGTGTATCGCGGCGCTGCCGGGGGCATGGGGGGGGCggggggggcgaaa                                                                                                  >  1:202151/1‑229 (MQ=255)
                                                                                                                                            cTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGGTAAAACGGGGTCTGGCTGGCGGCAGTTTTTCAGCGGTTTTAACGAGGCGCGGACGATGGACTGGCGGGGGCTGGGAGCGAATaa                                                                                                  >  2:737234/1‑239 (MQ=255)
                                                                                                                                                ggggtCGGGGGCATACGGTATTTATCGTCACCCAGGATCCGAAGGTCGTGGTCCAGGCGGGGGGGGTGACAGAAATTCGCGACGGCGAAATTGTGCGAAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAAt                                                                                               <  2:1245624/234‑1 (MQ=255)
                                                                                                                                                     ggggggCATAGGGTAATTTTGCCCCCCCACGACCCGCAGGGCGCTGCTCAGGCCGGGGGGGTGATCGAAATTCGCGACGGCAAAATTGGGCCCAACCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTa                                                                                          <  2:1246767/235‑1 (MQ=255)
                                                                                                                                                              ggcgTGATTCACCTCACCCACGGGCGGGGGGCCGCTGCTCCGGCCGGGCGGGTGATCGAAAGTCGGGCCGGCGAAATTGGGCGAAACCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGAc                                                                                <  2:195068/236‑1 (MQ=255)
                                                                                                                                                                     ttttCGTCACCCACGATCCGGAGGTCGCTGCACAGGCCGAGGGGGTGAACGAAATGCGCGACGGCAAAATTGGGCACTATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCt                                                                         <  2:168627/235‑1 (MQ=255)
                                                                                                                                                                     ttggcctcccccGCGATCCGCAGGTCGCGGCTCAGGCCGAGCGGGTGATCAAAATGCGGGACGGCGAAATTGTGCGCAATCCCCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCt                                                                         <  2:463691/229‑1 (MQ=255)
                                                                                                                                                                         gctCACCCGCGTCCCGCAGGTCCCTGCTCAGGCCGGGCGGGTGATCGAAATTCGCGACGGCGAAATTGGGCACAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGa                                                                    <  2:473030/237‑1 (MQ=255)
                                                                                                                                                                                cccGATCGCCAGGGCGCTTGTCAGGCCTAGCGGGTTAACTAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGTAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATtattat                                                             <  2:615522/237‑1 (MQ=255)
                                                                                                                                                                                cagagtcgggagtgcGCTGCCGAGGCCGGGCGGGGGATCGAAATCGGGGGCGGCGAAAGTGTGCCCAGTCCGCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATtattat                                                             <  2:1167596/225‑1 (MQ=255)
                                                                                                                                                                                caagATCCGCGGGGCGCTGCTGAGGCCGGGGGGGTGCTGAAAATTCGGGACGGGGAAATTGGGCGAAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATtattat                                                             <  2:362874/236‑1 (MQ=255)
                                                                                                                                                                                      cggagggccggtgccCAGGCCGGGGGGGTGATCGAAATTCGCGCCGGCGAATTTGGGCGCAATCCGCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTAt                                                       <  2:899032/228‑1 (MQ=255)
                                                                                                                                                                                        gCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCCGTTTGTCAGCGGGTTTAACGAGGCGCTGACGATGGCATGGCGGGGGCTGGCGGCGCATAAAAAGCGTGCTTTTCTTACCCTGCTGGGGGTTATTATCGGGAttt                                                     >  2:353159/1‑238 (MQ=255)
                                                                                                                                                                                               gctgctCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGa                                                                                                                                                                                                    >  1:992867/1‑91 (MQ=255)
                                                                                                                                                                                               gctgctCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGa                                                                                                                                                                                                    <  2:992867/91‑1 (MQ=255)
                                                                                                                                                                                                            ggggggTGATCAAAATTCGGGACGGCAAAATGGGCCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGt                                  <  2:66167/235‑1 (MQ=255)
                                                                                                                                                                                                             gCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCCGGCAGGGGATAAAAAGGGGCCTTTTCTGACCCTGCTTGGGGTTTTTTTTCGGATTTGGCTGGGGGGTTTcatttttg                                 >  2:934925/1‑232 (MQ=255)
                                                                                                                                                                                                                             cgggtcgtggAAATTGGTCGAAATCCTCCCGGCATTGAAAAAGTGAAGGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGAcgccgc                <  2:49101/230‑1 (MQ=255)
                                                                                                                                                                                                                                           tgtgCACAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGGCAACGCGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGc  <  2:49246/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
CACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGC  >  NC_000913/920774‑921247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: