Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,065,745 A→G 19.3% E54G (GAG→GGG)  agp → glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,065,7450AG19.3% 117.5 / 3.6 57E54G (GAG→GGG) agpglucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base A (16/29);  new base G (10/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 7.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGA  >  NC_000913/1065507‑1065982
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
tttgGCGAACTTGTTCCCGGAAAAAAGTCATTTTTCTGTCACACTCTTTAGGGATTGATAACAAAAGAGGTGCCAGGAATGAAAAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGa                                                                                                                                                                                                                                                  <  2:209637/239‑1 (MQ=255)
                    aaaaaaGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAATGCTCATGATGAGCCGCCATAACTTACGGGCGCCGCTGGGGAACAATGGCAGGGGGCTGGGGCAGTCGACGCCGAATAAAt                                                                                                                                                                                                                              >  2:291799/1‑239 (MQ=255)
                    aaaaaaGTAATTTTTCTTCCACCCTTTTTAGTGATTGAAAACAAAAGAGCTGCCAGAAATGAACAAACCGCTAATCGCCGCAGCTGGGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAAt                                                                                                                                                                                                                              <  2:1176675/239‑1 (MQ=255)
                       aagaaactttttCTGCCACCCTTTTTAATGATTGACAAAAAAAGGGGTGACAAGAATGAAAAAAACCCTAACCGCCGCAGGTGGGGTAGGGATAGTTTATCTCGCTTCAAACGCTCAGGAACAAACCGTACCGGAAGGCTATCAGCGGCAGCAAGGGCTCAGGGTGGGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGc                                                                                                                                                                                                                           <  1:1011829/230‑1 (MQ=255)
                       aaaGTCATATTTCTGTCACCCTCTTTAGTGATTGAAAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGGGGCAGGGATAGTTTTACTCGCTTAAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGc                                                                                                                                                                                                                           <  2:889639/239‑1 (MQ=255)
                                           ctctTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTAATCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTAAAGCAAATGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGGGTGCTGGGGGAGTCGACGCCGAATACATGGCCAGAATGGGAcctgtccctt                                                                                                                                                                                                       >  2:1011829/1‑230 (MQ=255)
                                              tttAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCCTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAACTGCTCATGATGAGCCCCCATAACTTACGCGCGCCGCGTGCGCACATATGCAGGGTGCTGGGGCAGTCTACGCCGACAAGAAGGCCAAAATagggccgcccgcggggg                                                                                                                                                                                                    >  1:700121/1‑225 (MQ=255)
                                                    gattgaaAACAAAAGAGGTGCAAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACt                                                                                                                                                                                               <  2:700121/238‑1 (MQ=255)
                                                     attgatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAAGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACCTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGGGCAGTCGACGCCGAAAAAATGGCCAGAATGGGACGTCCCCGAGGGGCACCTc                                                                                                                                                                                              >  2:115381/1‑238 (MQ=255)
                                                     attgatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGGTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGGAGGCTATCAGCCACAGCAAGAGCTCACTAAGAGCCGCCCTAACTTACGGGCGCCGCTCGGCGACCACGTGCGGGGGCTGGAGCCAGCGCAGCCGAATAAATAGCCCAAACGGGAGCGCCCCGGGGGGCagcacc                                                                                                                                                                                             >  1:25075/1‑234 (MQ=255)
                                                      ttttaaaaaaaaGGGGGGCAGGGGATGAACAAAATGATAAGCGCCGCGGTGGTGGGGGGGATTGTTTATCCCGCTTCAAGCGCTCAGGCAAAATCCGTACCGGAAGGCTATCAGCGAAAACAGGTGCTAATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGTGACGTCCCCGGTGGGCAACTca                                                                                                                                                                                             <  1:1118629/230‑1 (MQ=255)
                                                        gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCCTAACTTACGTGCGCCGCTGGCGAACAATGGCAGGTTGCTGGGGCAGTCGACGCCGAATAAATGGCCCGAATGGGACGTCCCCCGTGGGCAAATcacc                                                                                                                                                                                           >  2:286777/1‑238 (MQ=255)
                                                        gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGGGCAGTCGACGCCGAATAAATGGCCCGAATGGACCGCCCCCGCGGGCCAACTaacac                                                                                                                                                                                          >  2:607740/1‑237 (MQ=255)
                                                        gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACCCTCAGGCACAAACCGTACCGGAAGGCTATCACCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACTTCCCCGGTGGGCAAccactc                                                                                                                                                                                           >  2:1013092/1‑233 (MQ=255)
                                                         aaaaaaaaaGGGGTGCCAAGAAAAAAAAACACCTAAACCGCCGCAGGTGGGGCGGGGATATTTTTACTCGTTTCAATCGACAGGCCACAACCCGTACGGGAAGGCTACCAGCTACAGCAAGTGCTAAGGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTcaccac                                                                                                                                                                                         <  1:1040850/234‑1 (MQ=255)
                                                          tAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCCTAACTTACGTGCGCCGCTGGCGAACAAGGGCAGTGGGCTGGGGAAGTCGACGCCGAAAAAATGGCCAGAATGGGACGGCCCCGGGGGGCAAACCcccacc                                                                                                                                                                                        >  2:1250065/1‑239 (MQ=255)
                                                                aaGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAAATCACCCCCCAAgg                                                                                                                                                                                   >  2:1118629/1‑238 (MQ=255)
                                                                aaGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGGGTGCTGGGGCAGCTGACGCCGAATAAATGGCCAGAATTGGAAGACCCCGGTGGGAAACTCCCACCCAAAgag                                                                                                                                                                                  >  2:159849/1‑237 (MQ=255)
                                                                aaGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTAATCGCTTCAAACGCTCAGGCCAAAAACGTACCGGAAGGCTATCAGCTACAGCAAATGCTCATGATGAGCCGCCATAAATTACGTGGGCCCCTGGCGAACAATGGCAGTGGGGTGGAGCCATCGGACCCGCAAAAAAGGCCACAAAGGGACGGCCCCGGGGGGGAAACCAACACAAAAgag                                                                                                                                                                                  >  1:164953/1‑237 (MQ=255)
                                                                     gTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGGGAACAATGGAAGTGTGCTGGGGCAGTCGGCGCCGAATAAATGGCCGGAATGGGACGCACACGGGGGGGAACTCACCACCAAAGGTggggg                                                                                                                                                                             >  2:87484/1‑236 (MQ=255)
                                                                     gTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTAACGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCCTAACTTACGTGCGCCGCTTGCGAACAATGGCAGTGTGCTGGGGCAGTCGACCCCGAATAAATGGCCCGAATGGGAGGTCCCCGGTGGGCAACTCACCACCAAAGGGGGCGt                                                                                                                                                                             >  2:131808/1‑239 (MQ=255)
                                                                          attaaTAAACAAACCGCTAGTCCCCCCGTGTGGGGCAGGGATTGTTTTGCTCGTTTCACACCCTCAGCGCCAAACTGTCACGGAAGGCTACCAGCTAAAGGAAGGGGTGAGGAGGGACCGCCATAACCTACGGGCGCCGCTGGCGAACAATGGCAGGGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATTGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                        <  1:1083565/236‑1 (MQ=255)
                                                                            aaaaaaaCAAACCGCTAAAGCCCGCAGGTGTGGCAGGGATAGTTTTACTCCCTTAAACCGTCCAGGCAAAAACCGTCCCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGaa                                                                                                                                                                      <  2:562805/234‑1 (MQ=255)
                                                                             aaTGAACAAACCTCAAATCCCCGCAGCTGTGGCAGGGATAGTTTTCCCCCTTACAAACGCTCAGGCACAACCCGTACCGGAAGGCTATCAGCTACAGCAAGTCCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                        <  2:213351/236‑1 (MQ=255)
                                                                             aaTGAAAAAACCGCAAAACGCCGAGGCGGGGGCGGGGATAGTTTTACTCGCTTCAAACGCCCAGGCACAAACCGAACCGGAAGGTTATCAGCTACACGAAGTGCTCATGATGGGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                        <  2:1127041/236‑1 (MQ=255)
                                                                             aaTAAAAAAACCCCTAATCGCCCGAGCTGGGGCGGGGATAGTTTTACTCCCTTCAAACCCTCAGGCCCAAACCGTACCGGAAGGGTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                        <  2:586699/236‑1 (MQ=255)
                                                                             aaTAAAAAAAACGCTAACCGCCGCAGTGGGGGCAGGGATAGTTTTCCCCGCTTCAAACGCTCAGGCAAAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGaa                                                                                                                                                                      <  2:57223/238‑1 (MQ=255)
                                                                                   cAAACCGCAAATCGCCGCAGCTGGGGCAGGAATAGTTTTACTCGTTTCAACCGCTCAGCCACAAACCGTCCCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGtata                                                                                                                                                               <  2:164953/239‑1 (MQ=255)
                                                                                        cGCTAATGGCGGCAGGTGTGGCAGGGATAGTTTTCCTCGCTTCAAACGCTCAGGCAAAAACCGTACCGGAAGGCTATCAGCTACAGCAGGTGCTCATGAGGGGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGc                                                                                                                                                         <  2:535840/239‑1 (MQ=255)
                                                                                                               ggggTATTTTTACTCTCTTTAACCGCTGCGACCCAAACCGTACGGCAAGCGAATCAGCCTGAGGAAGGTATAGTGTGGGGCCGCGATAACTTACGGGCGGCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAAtggctgg                                                                                                                                  <  1:443675/238‑1 (MQ=255)
                                                                                                                      ttttACCCGCTTAAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCa                                                                                                                                <  2:915196/233‑1 (MQ=255)
                                                                                                                           cTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTCCCCGAAGTGTATATGGGCCCTTACTTGCGTAAATGCCTGGCGCGGCAGGGGAt                                                                                                                     >  2:98570/1‑239 (MQ=255)
                                                                                                                                ttCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGGGTATATGGGCCATTACATGCGTGAATGGCTGGCAGATCAGGGGTTGGGGa                                                                                                                >  2:1215320/1‑239 (MQ=255)
                                                                                                                                ttCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGGGCCCCAAGGGGAAATGGGCCATTTCATGCGCGAATGGCGGGGAAGGCAggggggatgtg                                                                                                                >  1:915196/1‑232 (MQ=255)
                                                                                                                                          catgCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCAGGCGACCAATGGCAGTTTGCTGGAGCAGTCGACGCCGAATAAATGTCCCGAATGGGACGTCCCCGGTAGGCAACCCACCACCCAAGGTGGCGTGTTCGAAACGCCTAAGGGCCATTACAGTGAGTAATGGGTGGCCGAGCGggggtggggggaatgagggga                                                                                                      >  1:857814/4‑222 (MQ=255)
                                                                                                                                          cAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTTCCCGGTGGGCAACTCCCCACCAAAGGGGGCGTGCTCGACGGGAAAATGGGCCATTCAATGCGTGAATGGCGGGACGGGCAgggggggggtgaaccggggga                                                                                                      >  1:784920/1‑222 (MQ=255)
                                                                                                                                                ccaAACCGTCCGGGAAGGCTACAAGCACAGGAAAGTGTTATGGAGGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGcc                                                                                                 <  2:25075/238‑1 (MQ=255)
                                                                                                                                                            ggAAGGCTGTCAGCTACAGCAAGTCCTCAGGATGACCCGCCATAACTTACGGGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTaca                                                                                     <  2:538338/239‑1 (MQ=255)
                                                                                                                                                            ggAAGGCTATCAGCTACAGCAGGGTATCATGGTGGGCCGCCATACCTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAATTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTaca                                                                                     <  2:205121/239‑1 (MQ=255)
                                                                                                                                                                    aTCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGGGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTtat                                                                            >  2:443775/1‑239 (MQ=255)
                                                                                                                                                                      cAGCTAAAGCAAGTGCTCATGATGGACCGCCAAAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGAGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGCGGGGCAACTCACCCCCAAAGGTGGCCGGCTCGAAGTGATATTGGGCCATTCAATGCGTGACTGGCGGTCAGAGCAGGGGAGGGGGAAACAGGTGAATTGCCGCCCGCCGAACCCCGttattg                                                                           >  1:1078507/1‑234 (MQ=255)
                                                                                                                                                                                    tGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGGGGGCAACTCACCACCAAAGGAGGCGAGCTCGAAGGGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAAGGGCCGCCGCCGCAAACCTTTTGTGCCTATGACAAAAg                                                              >  1:729625/1‑238 (MQ=255)
                                                                                                                                                                                                     cATAACTTCCGTGGGGCGGTTGCATACAATGGCCTTGGTCGGGGGCACGCGACGTCGAATAAATGGCCAGAATGGGACGGCCCCGGTCGCCAACTCACCACCAAAGGGGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGc                                            <  1:291799/239‑1 (MQ=255)
                                                                                                                                                                                                       aaacgtaccgtcgccccTGGCAAACAAAGGCAGTTGGCGCAAGCAGTTGTCCGCGAATAAATGGCCGAAATGTAACCTCCCCGGTGGGCACCTCAACACCAAAGGTGGCGTGTGTGAATTGTATTTGGTCCATTACATGCGTGAATGGCTGTCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGa                                          <  1:87484/228‑1 (MQ=255)
                                                                                                                                                                                                            tACGTTCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                     <  2:399610/239‑1 (MQ=255)
                                                                                                                                                                                                            tACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGGCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                     <  2:380825/239‑1 (MQ=255)
                                                                                                                                                                                                               gTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACCTGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAACTGGGGGAATGCCCGCCGCCGACACCCGTTTATGCCCTTGCCCAAAATCTGCACCGAACCGGTGCGACCGCCCAg                                  >  1:65068/1‑239 (MQ=255)
                                                                                                                                                                                                               gTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGAAGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAg                                  <  2:420914/239‑1 (MQ=255)
                                                                                                                                                                                                                  cgccgcTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATCAATGGACAGAATGGGACGTCCCCGGTGGGCAACTAACCACCAAAGGATGCGTGCTCGAAGTGAATATGGGCCATTACATGCGGGCAGGGCTGGCAACGACGGGGATGGGCAAATCGGGGTAATGCCACCCGCCGTGCACCGTTACTGCCCAGGAGAAAAACCTGCAACGTCCCGTTGAAACCgacaact                                 >  1:285113/1‑231 (MQ=255)
                                                                                                                                                                                                                            gtcaCAATGGCAGGTTGTTGGGTCAGTCGGCGCAGAAGAAATGCCCAGAATGGGAGGTCCCGGGGTGGCAACCAACATCCAAAGGGTGAGTGCGCGAAGTGTATGTGGGCCATTACATGCGTGAAGGGCTGGCAGCGCAGGTGATGGTGAAAGCGGGGGAAGGCCCGCAGCCGTAGACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTttatgaa                       <  1:778868/235‑4 (MQ=255)
                                                                                                                                                                                                                               aCAATGGGAGTTTGCTGGAGCAGTCGGCCCCGAATAAATGGCCAGAATGGGACGGCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGATCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCGATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCgg                    <  2:310642/238‑1 (MQ=255)
                                                                                                                                                                                                                                cAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGGGCAGGGGATGGGGAAATCGGGGGAATGCCCGGCGCCGTACACCGTTTATGCCTTTGCCCATAGTCTACAACGAACCGTTGCGACCGCCAAGGTCTTTAATAACcgcg                  >  1:619154/1‑239 (MQ=255)
                                                                                                                                                                                                                                cAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGgcg                  <  2:423482/239‑1 (MQ=255)
                                                                                                                                                                                                                                 aaTGGCAGTGGGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGGCGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGgcg                  <  2:921141/238‑1 (MQ=255)
                                                                                                                                                                                                                                        gggtgCTGGAGCAGTCGACGCCGAATAAAGGGCCAGAATGGGACGTCCCCGGTGGGCAACTACCCACCAAGGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTccc           <  2:590132/236‑1 (MQ=255)
                                                                                                                                                                                                                                         tgtgCTGGAACAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCg          <  2:689765/238‑1 (MQ=255)
                                                                                                                                                                                                                                         tgggcgggACCAGGCGACGCCGAATAAAGGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCg          <  2:668293/232‑1 (MQ=255)
                                                                                                                                                                                                                                                gAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTAAATGGCCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGGGAAACCGGGGGAATGCCCGCCGCCGTAGACCGCTTTGGCCTATGCCAAAAGGCTGCAACGGACCGCTGCGCCCGCAAGGTTCTTTATTACCgggcacatcaccgggggggg  >  1:721419/1‑221 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
TTTTGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGA  >  NC_000913/1065507‑1065982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: