Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,130,037 G→T 17.5% A31E (GCA→GAA)  flgM ← anti‑sigma factor for FliA (sigma 28)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,130,0370GT17.5% 183.5 / 7.8 96A31E (GCA→GAA) flgManti‑sigma factor for FliA (sigma 28)
Reads supporting (aligned to +/- strand):  ref base G (7/71);  new base T (16/2);  total (23/73)
Fisher's exact test for biased strand distribution p-value = 4.76e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCA  >  NC_000913/1129802‑1130274
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
tGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTtcc                                                                                                                                                                                                                                                   >  2:449596/1‑238 (MQ=255)
  gTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCTTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGGTGTTTTTgcctc                                                                                                                                                                                                                                                 >  2:522317/1‑236 (MQ=255)
  gTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGCCGGTTGGTTTTtcccc                                                                                                                                                                                                                                                 >  2:365883/1‑236 (MQ=255)
  gTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGATTTCCTTGCGCGTCGCTTAACGACACCATTGTGCTGGTGGAGGCGGTTGGTTTTgtcgc                                                                                                                                                                                                                                                 >  1:86233/1‑238 (MQ=255)
       ggATCTCTGCCAGACGTGTCATACGGTCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCCCCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTCCCGGGT‑TGCATCAGTTTTGCTTCCGCGCCGCTCAACGTCCCACTGGGGCTGGTGGAGGCGGTTGTTTTTGTCGCCCGTCt                                                                                                                                                                                                                                           >  1:229513/1‑239 (MQ=255)
          tctcctcaattcgtgcctttcGATCAGTTTCGTCTG‑AAGTGTGGCTGTGCTTCGTTGATCAGCGAGTCGGCAATTTTGCTCGTGTCCATTTTTAG‑TTCCCCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACGCTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑tt                                                                                                                                                                                                                                       <  1:967943/219‑1 (MQ=255)
                catggcgttgtaTACGATCTTTTAC‑TCTGCAAGTTTTGCTGCGCTTCGTTTATCCGCGCATCGGCCATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGCCACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTAc                                                                                                                                                                                                                               <  1:293746/228‑1 (MQ=255)
                     cggtttctaagATCAGTTTC‑CTTGCAAGTTTTGGTGCGCTTCGTTGGCAAGCGGATCGGCAATTTTGCCGGTTTCCATTTTTAG‑TTCACCGTTACGAATCGGCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCgg                                                                                                                                                                                                                            <  1:907550/227‑1 (MQ=255)
                        gtCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTTCCGCCCCGCCG‑GT‑CG‑TTaaccgccgc                                                                                                                                                                                                                        >  2:293746/1‑230 (MQ=255)
                        gtCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCCG‑TT‑CG‑TTTCCggggggc                                                                                                                                                                                                                       >  2:411457/1‑234 (MQ=255)
                        gtCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGATTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGAGGAGGCGGTTTCTTTTTCTGCCCGGCGG‑TT‑CG‑TTTCCgggcggt                                                                                                                                                                                                                       >  2:737278/1‑234 (MQ=255)
                        gtCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAAATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCCCCCTGGTGCTGGTGGAGGCGGGTGGTTTTTGCGCCCCGccgctccgttcgggggggg                                                                                                                                                                                                                          >  1:1278364/1‑221 (MQ=255)
                                 tCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTTCCCCCCGGCGG‑TT‑CGTTTACCGG‑‑GCGTCacagggtt                                                                                                                                                                                                             >  2:161356/1‑232 (MQ=255)
                                      tttctctggcggtttttctggtcTTTGTTTATCCGCGCTTTGGCTATTTGGGCGGGTTCCATTTTTAG‑TTACCCGTTACGAATCGTCAGTTTTAACGCTTCGACACGTTAAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGGCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTcgcg                                                                                                                                                                                                        <  1:442254/217‑1 (MQ=255)
                                      tAC‑TCTTGAAGTCTTGGTTCTGTTCATTGATCAGCGCATCGGCAATGTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATAGCACTGCTGCCGGGT‑TGCAGCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTcgcg                                                                                                                                                                                                        <  1:787885/238‑1 (MQ=255)
                                      tAC‑TCTGCAAGTCTTGCTGCTCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTcgcg                                                                                                                                                                                                        <  2:176684/238‑1 (MQ=255)
                                      gtcagtgtcAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTcg                                                                                                                                                                                                          <  2:31784/229‑1 (MQ=255)
                                       tacttggCAAGTCTTTCCGCGTTTGGTTGATCAGCCGATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGAAACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTcgcg                                                                                                                                                                                                        <  1:107194/232‑1 (MQ=255)
                                        c‑tctGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTTTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCAACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCgg                                                                                                                                                                                                     <  2:1279397/239‑1 (MQ=255)
                                        c‑tctGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCgg                                                                                                                                                                                                     <  2:435903/239‑1 (MQ=255)
                                          tctGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTGTTTTCCGCCCGCGTG‑GT‑CG‑TTTCCGG‑GGCGGCAGTGGGTTTCGCgggg                                                                                                                                                                                                     >  2:510030/1‑239 (MQ=255)
                                          tctGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGGGCTGGTGGAGTCGGTTTTTTTTTCCGCCCCGCTG‑TTCCT‑TTCCCGG‑CGCGGACGT‑GGtttgtcgggg                                                                                                                                                                                                     >  1:206520/1‑232 (MQ=255)
                                            tGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGGGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGAGGTTGTTTTTTGTGCCCGGCGG‑TT‑CG‑TTGCCGG‑CGCGtacggggggtagggcgggtt                                                                                                                                                                                                   >  1:93976/1‑220 (MQ=255)
                                            tGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTTCCGCCCGGCCG‑TT‑CG‑TTACCGG‑cccgcccgggggtttccgcgggtt                                                                                                                                                                                                   >  2:351513/1‑219 (MQ=255)
                                            tGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCCG‑TT‑CG‑TTTCCCG‑GGCGGCAGT‑GGGTTTGCGCGGGTg                                                                                                                                                                                                  >  2:211056/1‑239 (MQ=255)
                                            tGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGGTTTTTCCGCCCGGCTGTTT‑CT‑TTACCgggggtcccggggtttcgccgggtg                                                                                                                                                                                                     >  2:147969/1‑216 (MQ=255)
                                            tGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGGTGTTTTTGCCCTCCCGCCG‑TT‑CG‑TTACCGG‑CGCGGCCGG‑GGGTTCGCGCGGCTg                                                                                                                                                                                                  >  2:558772/1‑239 (MQ=255)
                                             ggaaGTCTTGCTGCGCTTCGTTGATCAGAGCATCGGCAATTTTGCCGGTGTCCATTTTTAT‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGa                                                                                                                                                                                                 <  2:647488/237‑1 (MQ=255)
                                             cgaaGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTTGCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGa                                                                                                                                                                                                 <  2:1014210/237‑1 (MQ=255)
                                               cttggttGCTCCGCTTCGGGTATCAGCCCCACTGGAATTTTGCCGGTGTCCTTTTTTAG‑TTTCCCGGTCCGAATCGCCAGTTTTAACGGTTCGACCCGTTTAAGTATGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGTTGTAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTATCGG‑CGACTCAGT‑GGTTTCGCGCTGTTGAAc                                                                                                                                                                                               <  1:510030/234‑1 (MQ=255)
                                                aGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGA‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGCGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTATCGG‑CGCGGCAGT‑GGGTTTGCCCGGGTGGACg                                                                                                                                                                                              >  2:793071/1‑239 (MQ=255)
                                                   cTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGGTTTTTCCGCCCGGCCG‑TT‑CG‑TTTCCGGCCGGGTGGGG‑GGTTTcgcgggtgtgaccgg                                                                                                                                                                                             >  2:103475/1‑227 (MQ=255)
                                                    ttGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGGTTTTTTCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTGTTCCGCGGGTGAACggg                                                                                                                                                                                            >  2:1280554/1‑236 (MQ=255)
                                                    ttGCTGAGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGTTTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTTGCTTAACCTAGCACTGTGGCGGGCGGAGTGGGTTGTTTTTTTCGCCCCGCCG‑GT‑GG‑TTACCGT‑CCCGTGAGT‑ggggtaccgcgggtgttcggggg                                                                                                                                                                                          >  1:207724/1‑218 (MQ=255)
                                                      ggtgcGCTTCGTTTATCAGCGCATTGGCATGTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCtt                                                                                                                                                                                        <  1:912691/237‑1 (MQ=255)
                                                      gctgcGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGGTGTTTTTTCCGCCCGGCTG‑TT‑CC‑TTTACGG‑GGCGCCAGG‑GGTGTCCGGCGGTTgtatgggggtt                                                                                                                                                                                        >  2:790051/1‑229 (MQ=255)
                                                      gctgcGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGCCGGTTGTTTTTTCCGCCCGGCTG‑TT‑CG‑TTTCCCG‑CCCGTCCCG‑GGTTTCGCGCGGGTTAAccgggcgt                                                                                                                                                                                        >  2:732334/1‑232 (MQ=255)
                                                       ctgcGTTTCGTTGATCAGCGCAGCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTa                                                                                                                                                                                       <  2:1035084/239‑1 (MQ=255)
                                                         tgccttcgtttgtccgggctttcgCAATTTTTCCGGTTGCCATTTTTAG‑TTCACCGTTACGAATCGGCAGTTTTAACGCTTCGACACGTTCAAGATTGATAGCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACa                                                                                                                                                                                     <  1:453117/216‑1 (MQ=255)
                                                         tcgtgtgtgtttttcggccATCGGGAATTTTGCCGGGTTCCATGTTTAG‑GTCCTCGTTACGAAGCGGCAGTTTTAACCGTTCGGTACGGTCAAGATTGATATCACTGCTTCCGGGT‑TGCATCAGTTTTGCGTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACa                                                                                                                                                                                     <  1:104542/221‑1 (MQ=255)
                                                         gcgcTTGGTTGATCAGGGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGAGTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACa                                                                                                                                                                                     <  2:231675/239‑1 (MQ=255)
                                                          cgcTTCGTTGATCAGCGTATCGGCAATTTTGCCGGTTTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAg                                                                                                                                                                                    <  2:219873/239‑1 (MQ=255)
                                                                         cgcATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCCG‑TT‑TG‑TTTCCGG‑CGCGCCAGG‑GGGTTTGCGCGGGTGGACCGGGGTTTCAGGCtttccaggggaga                                                                                                                                                                     >  2:352071/1‑228 (MQ=255)
                                                                         cgcATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGGTGGTTTTTCCGCCCGGCTG‑TT‑CG‑GTTCCGG‑CGCGCCACG‑GGTTTTGCGCGGGTTAAAGGGGCTTCACGGCGTCGGAGG‑GGaaa                                                                                                                                                                    >  2:1266413/1‑238 (MQ=255)
                                                                          gcATCGGATTTTTTTCCGGTGTCTCTTTTTTG‑TTTACCTTTACGAATCGCGAGTTTTAACGCTTCGACACGGTCAAGATTGATATCGCTGCGGCCGGGT‑TGCAGCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGt                                                                                                                                                                   <  1:161356/239‑1 (MQ=255)
                                                                            ccgcgatgttttGCGGGT‑TCCTTTTTTGA‑TTCACCCATACGAATCGCCGGTTTTAACTCTTCGGCACGTTCAAGATTGATAACCGTGCTGGCGGGT‑TGCATCAGTGTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTTGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTAACGG‑CGCTTCAGT‑GGTTTCGCGCGGTTGAACGGGGCTTACAGGCTTCAGAGG‑GGAAGAGCg                                                                                                                                                                <  1:666514/231‑1 (MQ=255)
                                                                                tcatttttGCCTGTTCCCTTTTTTTG‑TTCCCCGTTTCGAATCGCCAGTTTTAACGCTTCCACAGGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCAGCAGTTTTGCGTGCGAGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATc                                                                                                                                                             <  1:1182476/235‑1 (MQ=255)
                                                                                   tattgggcGGTGTCCATTTTTAG‑TTCCCCCTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCaa                                                                                                                                                           <  2:842250/231‑1 (MQ=255)
                                                                                       tGCCGGTTGCCATTTTTAG‑TTCCACCTTACAAATCGCCAGTTTTAAATGCGCGACACGTTTAAGATTGATATCACTGCGGCCGGGT‑TGCATCAGTTTTGGTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACCGGCTTCAGAGG‑CGAAGTGCGATCAATACTc                                                                                                                                                      <  1:1282619/239‑1 (MQ=255)
                                                                                        ttctgtttCCATTTTTTG‑TCTACTGTTTCGACACGCCAGTTTTTACGCTTGGAGTCGTGTGAGATTGTGATCCATGCTGCCGGGT‑TGGATCAGGTTTGCTTTCTCGTTGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGGCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCTTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTTAGAGG‑CGAAGTGCGATCAATACTCa                                                                                                                                                     <  1:558772/232‑1 (MQ=255)
                                                                                        tgcgggcgccATTTTTTG‑TTGCCCGTTATGAATCGCTTGTTTTAACGCTTCGTCACTTTCAAGATTGATACCGCTGCTGGCGGGT‑TGCAGCTGTTTAGATTGCGCGTCGCTTAGCGTCACACTGGTGCGTGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑AG‑TTTCCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCa                                                                                                                                                     <  1:1182863/231‑1 (MQ=255)
                                                                                        ggcGGTTTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGAAACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCa                                                                                                                                                     <  2:1180879/237‑1 (MQ=255)
                                                                                        gcctgttcccttttttAGTTTCCCGGTTAC‑AATCGCCAGTTTTAACGGTTCGACGCGTTCAAGATTGATACCCCTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCGGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCa                                                                                                                                                     <  1:411457/228‑1 (MQ=255)
                                                                                        gCCGGTGTCCATTTTTAG‑TTCCCCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCa                                                                                                                                                     <  2:229513/239‑1 (MQ=255)
                                                                                        gCCGGGTTCCATTTTTAG‑TTCCCCGTTACGAATCACCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCa                                                                                                                                                     <  2:1020722/239‑1 (MQ=255)
                                                                                        gCCGGGTTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTGTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCa                                                                                                                                                     <  2:891739/239‑1 (MQ=255)
                                                                                          ctttgtCCCTTTTTAG‑TTTACCGTTACGAATCGCAAGTTTTAACCCTTCGACCTGTTGAAGGTTTGTACCCCGGGTGCCGGGT‑TGCATCGAGTTTGCTTGCGCGTCGCTTAGCGGCACACTGGTGCTGGGGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑GG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGT‑CGAAGTGCGATCTATACTCATg                                                                                                                                                   <  1:790051/236‑1 (MQ=255)
                                                                                             tgatcttttttCG‑TTCCCCGTTCCGCATCGCTAGTTTTAACGCTTCTACCCGTTCAAGATTGGTATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTT‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGt                                                                                                                                                 <  1:1011344/232‑1 (MQ=255)
                                                                                               tttctttttttttttcccttttcGAATCGCCGGTTTTAACGCTTCGACCCGTTCAAGATTGATATCTCGGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGttt                                                                                                                                               <  1:908804/217‑1 (MQ=255)
                                                                                               ttctttttttgtttccctcttACGATTCGTCAGTTTTAACGCTTCGGCTCTTTCAAGCTTGATATCCGTGCTGTCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACTCTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGGTG‑TT‑CG‑TTACCGG‑CGCGTTAGT‑GGTTTCGCGCGGTTGATCGGTGATTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGttt                                                                                                                                               <  1:163740/220‑1 (MQ=255)
                                                                                               tCCTTTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACTCGTTCAAGGTTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCCTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTa                                                                                                                                              <  2:639019/239‑1 (MQ=255)
                                                                                                  aTTTTGAG‑TTCACCGTTACGAATCGCCATTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTGTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTc                                                                                                                                           <  2:86233/239‑1 (MQ=255)
                                                                                                        aG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCAtt                                                                                                                                     <  2:706984/239‑1 (MQ=255)
                                                                                                              tcccttACGATTCGCCAGTTTTAAAGCTTCGACACGTTCAAGATTGATATCACTGGTGCCGGGT‑TGCACTAGTTTTGCTTGCGCGGCGCTTAACGTCTCACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAggg                                                                                                                                <  1:1114753/235‑1 (MQ=255)
                                                                                                              ccctttccgattcctcgtttttAACGGTTCTGCTCGTGAAAGTTTGTTTTCCCTGCTGCCGGTT‑TGCATCAGTTTTTCTTGCGCGTCGCTTAGCGTCACACTGGTGCTTGTGGAGGCGGTTGTTTTTGCCGCTCGTCTG‑GT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGAGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTACTCCTCATTGAagg                                                                                                                                <  1:737278/221‑1 (MQ=255)
                                                                                                                cttttcgaagtctcAGTTTTAACCCTTCGACACGTTCAAGATTGGTATCCCGGCGGCCGGGT‑TGGATCAGTTTTGCTTGCGCGTCGCTTAACGTTACACTGGTGCGGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGgcg                                                                                                                              <  1:799607/226‑1 (MQ=255)
                                                                                                                cGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGgcg                                                                                                                              <  2:206520/239‑1 (MQ=255)
                                                                                                                cGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGgcg                                                                                                                              <  2:857889/239‑1 (MQ=255)
                                                                                                                cGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGgcg                                                                                                                              <  2:199680/239‑1 (MQ=255)
                                                                                                                  tttcGACTCGCCAGTTTTTACGCTTGTAACCGTTCAAGATGTTAATCGCTGGTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCt                                                                                                                            <  1:147969/236‑1 (MQ=255)
                                                                                                                   tACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCtt                                                                                                                           <  2:1278364/239‑1 (MQ=255)
                                                                                                                        actgCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATc                                                                                                                      <  2:272817/236‑1 (MQ=255)
                                                                                                                            cctgTTTTAACGTTTGCACACTTGCAAGATTGGTATCCCTGCTGCCGGGT‑TGCTTCAGTTTTTTTTGCGGGTCGCTTAACGTCGCACTGGTGGTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCAtgt                                                                                                                  <  1:1226327/236‑1 (MQ=255)
                                                                                                                             tgttttttaggtttCGACACGTTCAAGATTGATTTCCCTGCATCCGGGT‑TGCATTGGTTTTGCTTGCGCGTCGCTTAACTTCACACTGGTGCTGGTGGAGTCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCAagtg                                                                                                                 <  1:423474/227‑1 (MQ=255)
                                                                                                                                  tttaCGCTTCGGCATTTTCAAGATTTATATCACTGCTTGCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCt                                                                                                            <  1:487340/236‑1 (MQ=255)
                                                                                                                                     aCGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCttat                                                                                                         <  2:246605/239‑1 (MQ=255)
                                                                                                                                              cacTTTCAAGATTGATATCCCTGGTGCCGGGTGTGCA‑CGTTTTTGCTTGGGGGGCGCTTAACGTCCCACTGGTGCTGGGGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCa                                                                                                <  1:667337/239‑1 (MQ=255)
                                                                                                                                                         ttGATATCACTGCTGCCGTGT‑TGCATCTGTTTTGCTTGCTGGTCGCTTAACGTCACGCTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACggg                                                                                       <  2:207724/237‑1 (MQ=255)
                                                                                                                                                         ttGATATCACTGCTGACGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTa                                                                                     <  2:847119/239‑1 (MQ=255)
                                                                                                                                                                 ccttctgctgGGT‑TGCGTCTTGTTTGCTGGGGCGCCGGTTTACGTCCCCATGGTGCGGGTGGAGGCGGTTGTTTTGGCCGCCCGGCTG‑TT‑CG‑TTGCCGG‑GGCGTCAGT‑GGGTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGAGGGGTAATCTTTaa                                                                             <  1:1266413/235‑1 (MQ=255)
                                                                                                                                                                 aCTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCaa                                                                                                                                                           <  2:925178/161‑1 (MQ=255)
                                                                                                                                                                 aCTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGGTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTTAGGGCGCTTTTTTCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTaa                                                                             >  2:1221662/1‑239 (MQ=255)
                                                                                                                                                                         cGGGT‑TTCATCAGTTTTGCTTGCGCGTCGCTTAACGTCCCACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTAc                                                                     <  2:93976/239‑1 (MQ=255)
                                                                                                                                                                         cGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTa                                                                      <  2:813826/238‑1 (MQ=255)
                                                                                                                                                                          cggttttgttctgTTTTTCTTGCGGGTTGTTTAACGGAACACTGGTGCTGGGGGAGGCGTTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTAc                                                                     <  1:1280554/227‑1 (MQ=255)
                                                                                                                                                                             t‑tGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGGTTTTTGCGCCCGGCTG‑GT‑CG‑TTACCGG‑CGCGGCCGG‑GGGTTTGCCCGGGTTTAGGGGGCTTCCATGGTTTAGCGG‑GCACGGGGGGGCAAAATCCATGGGTATTTCCCCCTTGGTGGCGCTTTTTTCCTGTGTTGTTGTTTTTTCCGGAAGGGACGGGtgactttttaaacggttacggg                                                                 >  1:786696/1‑218 (MQ=255)
                                                                                                                                                                                   tCAGTTTTGCTTGCGTGGCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTtat                                                             <  2:98134/238‑1 (MQ=255)
                                                                                                                                                                                        tttGCTTGCGCGTGCCTTAACGTTACACTGGTGCTGGTGGAGGGGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTTCCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATTCTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGa                                                         <  2:646125/237‑1 (MQ=255)
                                                                                                                                                                                                              tgtcgttggttttggttgGGGTGGTTGTTTTTTCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCCTGAGT‑GTTTTCGGTCGGTTGAACGGTGCTTACAGGCTTAAGAGG‑CGAAGTGCGATCAATTCTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGTCAAGGGACGTGTAATCTTTAACAGCTTACAGTTTTATAAGAATATTCCCATCTGCATCAACAAcg                                 <  1:336522/227‑1 (MQ=255)
                                                                                                                                                                                                               cacgtcgtggcgggtggAGGCGTTTTTTTTTGCCGCTCTGCTT‑GT‑CG‑TTGCCGG‑GGCGTGGGT‑GGTTTTGCGCGGTTGAACGGTGCTTACAGGCATCAGGGG‑CGAAGTGCGATGAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTGTATAAGAATATTCCCATCTGCATCAACAAcga                                <  1:705601/227‑2 (MQ=255)
                                                                                                                                                                                                                 ctcggttggtggtggAGGGGGTTGTTTTTGCCGTCCGGGTG‑TT‑TG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCTGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATTCCCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCCTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAAcgccg                              <  1:896561/230‑1 (MQ=255)
                                                                                                                                                                                                                  acTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTaa                                                                             <  2:454905/191‑1 (MQ=255)
                                                                                                                                                                                                                    tggtgctggtgGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTg                           <  2:786696/239‑1 (MQ=255)
                                                                                                                                                                                                                    tggggctggggGGGGGGGTTTTTTTGGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGGGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTg                           <  1:103475/239‑1 (MQ=255)
                                                                                                                                                                                                                          tggtgtGGGGGGTTGTTTTTGCCTCCCGGCTG‑TT‑TG‑TTACCGG‑CGCGTCAGT‑GGTTGCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTAcc                     <  1:352071/239‑1 (MQ=255)
                                                                                                                                                                                                                          tggtggAGGCGGTTGTTTTTGCCGCCCGGCTT‑TT‑CG‑TTACCGG‑TGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTAcc                     <  2:1195795/239‑1 (MQ=255)
                                                                                                                                                                                                                          gggtggGGGTGGTTGTTTGTGCCGCCGGGCTG‑TT‑CG‑TGTCGGG‑GGTGTTAGG‑GGTTTCGGGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATATTCATGTTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCGTACAGGTTTATAAGAATATTCCCATTTGCAGCAATACAGCCGGTGGCTAcc                     <  1:732334/238‑1 (MQ=255)
                                                                                                                                                                                                                           ggtggGGGGGTTTTTTTTTTCCGCCCGGCTT‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTAcc                     <  1:1221662/238‑1 (MQ=255)
                                                                                                                                                                                                                                           tctGCCGCCCGGGTT‑GT‑TG‑TTACTGG‑CCCTGTAAT‑GGTTTCGCGCGGTGGAATGGTGTTTACCGGCTGCAGGGGTCGAGGAG‑GATGAATCCCCATGGTTTATTCCTGATTGATGTAGCTTTTATAATGTGTTGCTTATTTATCGGCCAGGGACGGGTTATCTTTAACAGCCTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATgcg    <  1:449369/237‑1 (MQ=255)
                                                                                                                                                                                                                                             tGCCGCCCGGCTG‑TT‑GG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCa  <  2:1188929/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
TGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTAC‑TCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAG‑TTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGT‑TGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTG‑TT‑CG‑TTACCGG‑CGCGTCAGT‑GGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGG‑CGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCA  >  NC_000913/1129802‑1130274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑