Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,435,725	C→A	12.3%	intergenic (‑106/+35)	uspF ← / ← ompN	stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,435,725	0	C	A	12.3%	217.3 / 2.1	74	intergenic (‑106/+35)	uspF/ompN	stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base C (56/8);  new base A (2/8);  total (58/16)
Fisher's exact test for biased strand distribution p-value = 3.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACACCTGCCGGGTTGTCTGCACC  >  NC_000913/1435488‑1435960                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              cAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCa                                                                                                                                                                                                                                              >  2:234292/1‑239 (MQ=255)    tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCGGaa                                                                                                                                                                                                                                           >  2:484260/1‑239 (MQ=255)    tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAAATAAAAGAAGAAAAAAAAAAAAGaa                                                                                                                                                                                                                                           >  1:38450/1‑239 (MQ=255)                    aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACa                                                                                                                                                                                                                           >  2:398017/1‑239 (MQ=255)                                 ctGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGC‑AGggagga                                                                                                                                                                                                             >  2:546432/1‑239 (MQ=255)                                    catcatCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCCCATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGC‑CAGGAGGAttt                                                                                                                                                                                                          >  2:324100/1‑239 (MQ=255)                                       atgtttataagagacagtCCTCAACGTGGCTAATCACGTGTTGAGTTAATTCCGAATCGGAAATAGCGATAGGGAAAAGAA‑TCGTTCTGTTCATAAACCCTCCTT‑TTTTAGTATCCGCAAAAAG‑TGTAACGCAAGATGACTTTTTTTGTGA‑AATGACGGAGTTCACTTTTTTAATTTAGATAAAAGGAGGAAGAAAAAGCAAAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAg                                                                                                                                                                                                        <  2:591573/221‑1 (MQ=255)                                         tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAT‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTGAATTTAGTTCAAGGAAGGAAGAATAGGCGGACAAATCCCGCATTAAAACCGGC‑Aaggagacattaaaac                                                                                                                                                                                                     >  2:556896/1‑229 (MQ=255)                                                 tGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGAAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATaaa                                                                                                                                                                                              >  2:917993/1‑238 (MQ=255)                                                       ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGCAGAATAAGCAGAAAAAGCCCGCCCTAACACCGGG‑CAGGGGGATTTAAAACTGAAAAAACAAAcc                                                                                                                                                                                       >  1:374900/1‑239 (MQ=255)                                                       ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATaaaccaaacc                                                                                                                                                                                       >  2:877213/1‑239 (MQ=255)                                                                 tGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGCCa                                                                                                                                                                             >  2:64747/1‑239 (MQ=255)                                                                  ggCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCTGCCATAACAGCGGG‑CAGGAGGATTTAGAATTGTTAAACCAGACCTAAAGCGACaa                                                                                                                                                                            >  2:994890/1‑239 (MQ=255)                                                                  gcgtAACCACGCGTGGAATTAATTCTGAATCGAAAATATCGATAGGGAAAAGAA‑TCTTTCTGTTCAAAAACCCTCCTG‑TTTTAGTATCCGCAAAAAG‑TTTAACGCCAGAACACCTTTTTTGTGA‑AATGAGGGATTTCACTTTTTTAATTTAGAAAAAAGGAGGAAAAATAAGCAGAAAAGGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                                                                                                                                                             <  2:355919/235‑1 (MQ=255)                                                                   gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATTAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAAAAATAATaaaactgtctctaaaaaacaac                                                                                                                                                                           >  1:591573/1‑220 (MQ=255)                                                                   gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACaaa                                                                                                                                                                           >  2:1286294/1‑238 (MQ=255)                                                                   gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAAAGCGGGACAGG‑GGATTTAAAAAAGAAAAAACAAACCTaaaacaaaaaa                                                                                                                                                                           >  1:573139/1‑231 (MQ=255)                                                                           tataaagaaaaatttatagatgagAAAAATCGATAGGGGAAAAAA‑TTCTTTTTTTTAAAAAACCCTCTT‑TTTTATGTTACGCAAAAAA‑TGATACCCCAGAAGAAATTTTTTGTGTAAAGGGGGGTTTTCACTTTTTTATTTAAAAAAAAAGGAGAAAAAATAAGAAAAAAAACCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCg                                                                                                                                                                    <  1:763888/216‑1 (MQ=255)                                                                           cgcgTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCgt                                                                                                                                                                   >  2:217511/1‑238 (MQ=255)                                                                             cgTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGAAGGATTTAGAACTGATAAACCAGACCTAAAGAGACAATATCATCgtga                                                                                                                                                                 >  2:47432/1‑236 (MQ=255)                                                                             cgTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑AGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCACGCCATAAAAGCGGG‑CAGGAGGATTTAGAAAGGATAAACAAGACCCAAAACGaaaaaaaaatgggag                                                                                                                                                                 >  1:1087257/1‑228 (MQ=255)                                                                                        ttCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCCtt                                                                                                                                                      >  2:1111319/1‑239 (MQ=255)                                                                                        ttCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAAAAACATCGGAAAAGAAGCCAta                                                                                                                                                      >  1:29052/1‑238 (MQ=255)                                                                                            gAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGCGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGACCTGATAAACCAAACCTAAAACGACAATATCACCGGAAGAGATGCCATTGGCa                                                                                                                                                  >  1:927215/1‑239 (MQ=255)                                                                                            gAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCa                                                                                                                                                  >  2:466423/1‑239 (MQ=255)                                                                                             aaTCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCa                                                                                                                                                  >  2:1170720/1‑238 (MQ=255)                                                                                                  gAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGc                                                                                                                                                          >  2:763888/1‑225 (MQ=255)                                                                                                  gAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGTCA‑GCGTaa                                                                                                                                           >  2:882685/1‑238 (MQ=255)                                                                                                       atCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGaa                                                                                                                                         >  2:798505/1‑236 (MQ=255)                                                                                                       atCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGaa                                                                                                                                         >  2:984536/1‑236 (MQ=255)                                                                                                         tgaaaGGGAAAAAAATTCGTTCTTTAAAAAAACCCTCCTT‑TTTTAGTACCCGAAAAAAG‑TTAAACGCCAGATGACATTTTTGGTGT‑AAGGAGGGAGTTCACTTTTTTAATTAAGATAAAAGGAGAAAGAAAAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTg                                                                                                                                     <  2:927215/235‑1 (MQ=255)                                                                                                                   aaGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTc                                                                                                                                   >  2:1179129/1‑230 (MQ=255)                                                                                                                           gTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACa                                                                                                                                                                                                                           >  2:707714/1‑137 (MQ=255)                                                                                                                           gTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATcctgtctctttta                                                                                                                    >  2:381521/1‑226 (MQ=255)                                                                                                                           gTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAACCTGTCATCTTCATCCAACAgg                                                                                                                   >  2:468233/1‑239 (MQ=255)                                                                                                                            ttatgtaaaaaaacCCTCCTG‑TTGTAGGATCCAAAAAAAG‑TATAACGCTAAAGCACTCTTTTGGGTA‑AATGACGGAGTTCACATTTTTATTTAAGAAAAAAGGAGAAAAAAAAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGt                                                                                                                  <  2:782870/229‑1 (MQ=255)                                                                                                                            ttCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCC‑GCGTAGAAGCTGTCAACTTCATCCAAAAgat                                                                                                                  >  1:192239/1‑237 (MQ=255)                                                                                                                             ttcttttaaaaaaCCTCTTTTTTTTAGTA‑CCACAAAAAGTTGGAACGACGGATGACTTTTTTGGTGT‑AGTGACGGATTACATATTTTTAATTTAGATAAAGGGAGGAAAAAAAAACAAAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGt                                                                                                                  <  2:938690/230‑1 (MQ=255)                                                                                                                                      aaaaCCTCCTT‑TTTGACTACCCGAATAAAG‑TGTAACCCCAGATGACATTTTTTGGGT‑AATGACGGATTTCTCATTTTTAAGTGAGATCAAGGGAGAAAGATGAACCAGAAAAAGCACGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATACCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTtata                                                                                                        <  2:160640/239‑1 (MQ=255)                                                                                                                                      aaaaCCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAAc                                                                                                                                                                                             >  2:647337/1‑155 (MQ=255)                                                                                                                                      aaaaCCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATg                                                                                                                                                           >  2:777460/1‑189 (MQ=255)                                                                                                                                        atcctcctT‑TTTAAGGACCCGCATAAAG‑TGTAACGCCAGATGACGTTTTTTGTGT‑AATGACGGAGCTCAAATTTTTAATTTAAATAAAAGGAAAAAGAATAAGAAGAAAAAGCCCGCCATAACGGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGt                                                                                                      <  2:615857/237‑1 (MQ=255)                                                                                                                                           ctcctG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CCGGAGGATTTAGAAATGATAAACCAGACCTAAAGCCACAAAATCATCGGTAAAAATGCAATTGGCA‑CCGTACAAGCTGCCATCTTAATCCACCAGGTTGGtttttaagacaa                                                                                                   >  1:1069340/1‑231 (MQ=255)                                                                                                                                           ctcctG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGGTTTTAAAGTCaa                                                                                                   >  2:429955/1‑239 (MQ=255)                                                                                                                                           ctcctG‑ATTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACATCGGG‑CATGAGGATTTAGAACTGATAAACCAGACCTACAGCGACAATATCACCGCAACAGCTGCCATTGGCATGCG‑ACAAGCTGTCATATTCATCCAAAAGGTTGATTTTATAGTcca                                                                                                   >  1:779200/1‑237 (MQ=255)                                                                                                                                             cctG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGCCCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGGTGCTTTTATAGTCaaag                                                                                                 >  1:890771/1‑237 (MQ=255)                                                                                                                                              ctG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACgg                                                                                                >  2:1037297/1‑238 (MQ=255)                                                                                                                                                  ttttAGTACCCGCATAAAT‑TAGACCGCAAGATTGCTGTTTTTGTGT‑AAGCGAGGATCTCAATTTTTAAATTTAAAAAAAAGGAGAAAGAATAAGAAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAgg                                                                                             <  2:661797/239‑1 (MQ=255)                                                                                                                                                            cGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                                                   >  2:446550/1‑239 (MQ=255)                                                                                                                                                            cGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                                                   >  2:889300/1‑239 (MQ=255)                                                                                                                                                            cGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                                                   >  2:1244973/1‑239 (MQ=255)                                                                                                                                                            cGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGAAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGCCATGtt                                                                                   >  2:312382/1‑239 (MQ=255)                                                                                                                                                                       gtAACGCCAGATGACACTTTTTGTGT‑AATGACGGGGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGCTTTAGAACTGATAAACCAGACCTAAAGCGACACTATCAACGGTAGAGATGCCATTGGTA‑CAGTAGATGAGGTACACTTCATCCAACAAGTTGATTTTATATTCCAGACAGAGGGAAATGTTTTTATTTaaa                                                                         >  1:1102407/1‑239 (MQ=255)                                                                                                                                                                       gtAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTTCAA‑ACTCAGAAACTTCCATCATTATCCAACAGGTCGATTTTTCAGTCAACGAGAGGGGACAGTTTTTTTTTaaaa                                                                         >  1:1077237/1‑239 (MQ=255)                                                                                                                                                                          aCGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAg                                                                      >  2:701915/1‑239 (MQ=255)                                                                                                                                                                          aCGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCAATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                                                                                                                                                 >  2:670313/1‑133 (MQ=255)                                                                                                                                                                                gATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAAAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGACATCTTCATCCAACAGGTTGATTTTATAGTCACAGAAGGGGGAAATGTTTTTATTGAAAAAATaaatg                                                                >  1:583136/1‑236 (MQ=255)                                                                                                                                                                                  tgacttttttgGTGT‑AATGCGGGAGTTCAAATTTTTAATTTAAAACAAAGGAAGAAGAAAAAGAAAAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGt                                                                 <  2:1124760/230‑1 (MQ=255)                                                                                                                                                                                  gaacttttgtggtga‑AATGAGGGAATTCAAATTTTAAATTAAGAAAAAAGGAAAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGt                                                                 <  2:1069340/225‑1 (MQ=255)                                                                                                                                                                                      acTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCa                                                          >  2:313466/1‑239 (MQ=255)                                                                                                                                                                                             tggaaaaTGAGGAATTTAACATTTTAAATTTAAAAAAAGGGAGAAAAAAGAAGAAAAAAACGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCg                                                    <  2:890771/234‑1 (MQ=255)                                                                                                                                                                                                t‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGt                                                >  2:514498/1‑239 (MQ=255)                                                                                                                                                                                                   aTGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTTGCGCCAATATCGGCGGAt                                              >  2:1048915/1‑239 (MQ=255)                                                                                                                                                                                                           gTTCACATTTTAAATTAAGAAAAAAGGAGAAAGAATAAGCAAAAAACGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAg                                      <  2:29052/239‑1 (MQ=255)                                                                                                                                                                                                           atctaaaTTTTAAATTTAGAAAAAGGGGGGAAAAATAGCAAAAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCa                                       <  2:1299145/232‑1 (MQ=255)                                                                                                                                                                                                            ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGAATTTAAccct                                      >  2:1104629/1‑236 (MQ=255)                                                                                                                                                                                                                  tttttAATTAAGAAAAAAGGAGGAAGAAAAAGAAGAAAAAGCCCGCCATAACAGAGGG‑CAGGAGGATTTAGAACTGAGAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGGCATCTTCATCCAACAGGTTGATTTTATAGTCAACTTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTa                               <  2:1254491/239‑1 (MQ=255)                                                                                                                                                                                                                    tttAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatc                             >  2:1095832/1‑239 (MQ=255)                                                                                                                                                                                                                            agacaaaaGGAAAAAAAATAAGCAAAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAacac                     <  2:414197/234‑1 (MQ=255)                                                                                                                                                                                                                             gATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAacac                     >  2:40729/1‑238 (MQ=255)                                                                                                                                                                                                                                   aggaggAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAATTAGTAAGTCGCGCCACTTTCGGCGTATTTAACCGGATCTTTATCATCAACACCTCCCgg              >  2:510272/1‑239 (MQ=255)                                                                                                                                                                                                                                   aggaggAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGACCTTTCTCATCACAACCTGCCgg              >  2:333394/1‑239 (MQ=255)                                                                                                                                                                                                                                        gaagaaTAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGGGGATTTAACCAGATCTTTATCATAAACACCTGCCGggtggt         >  1:768923/1‑239 (MQ=255)                                                                                                                                                                                                                                                aGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGCATTTAACCAGATCTTTATCATCAAAACCTGCCGGGTTGTCTGcacc  >  2:1060637/1‑238 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              CAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAA‑TCGTTCTGTTCATAAAACCTCCTG‑TTTTAGTATCCGCATAAAG‑TGTAACGCCAGATGACACTTTTTGTGT‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGG‑CAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCA‑GCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACACCTGCCGGGTTGTCTGCACC  >  NC_000913/1435488‑1435960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑