Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,616,800	G→A	14.6%	V346V (GTG→GTA)	yneK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,616,800	0	G	A	14.6%	124.7 / 2.5	57	V346V (GTG→GTA)	yneK	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (31/17);  new base A (2/7);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 2.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACTCACCCGATAATCTGTTATGACAAC  >  NC_000913/1616562‑1617035                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             gCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTCAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTg                                                                                                                                                                                                                                              >  2:68827/1‑239 (MQ=255)            cTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCCCCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGcc                                                                                                                                                                                                                                   <  2:478491/239‑1 (MQ=255)            cTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAACCAGGc                                                                                                                                                                                                                                    >  2:1152280/1‑238 (MQ=255)               ttggcagcaCATCAATCTCCTCTTTTTCTGAACTTGTTTTTATTTTTCACCACTATGTTATTGCACTATCACAAACTTATGAATCCCAATGAAAGTGATTTGACTGAGGTGT‑TGAGCCCAGCATAATTAAGCGATGATTACATATTAAAACTTATGGCGCGA‑C‑GGACCCTACAACTGTGTGCATAAAATTAAAAACCACCAAAAGCAC‑CTAAAGAAGACCTGTTGaaaaaaaaaccaa                                                                                                                                                                                                                                 >  1:426311/4‑230 (MQ=255)               ttAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCACTGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑C‑GTACCCTCAAACTATTTGTAAAAAATGAAACACCTCCCAAAGTTA‑TCAACAAAGCCCTGAGACAAAACAAGCCTc                                                                                                                                                                                                                                 >  2:826795/1‑238 (MQ=255)                  gCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTc                                                                                                                                                                                                                            <  2:1083350/239‑1 (MQ=255)                            aaTCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGa                                                                                                                                                                                                                  <  2:952203/239‑1 (MQ=255)                             aTCTCCTCATTTTCAGAATTTTTTTTTATTTTACCCCAATTTTTTATTGCCCTATCACCAATGTATGAAACCAAATTAAAGTTATTTGAATGATGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGac                                                                                                                                                                                                                 <  1:1242478/239‑1 (MQ=255)                                tcctcATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGacca                                                                                                                                                                                                               >  2:531745/1‑238 (MQ=255)                                tcctcATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAATTA‑ACCACGAAAACGTGGTGAAAAACAGCCCTC‑ACCCCCCGGTCAGacacc                                                                                                                                                                                                              >  1:818102/1‑236 (MQ=255)                                   tcATTTTGAGAACTTTTTTTTATTTCTCGCAATTATTTTATTGCACTATCACCAATTTATAAATCCAAATGAAATTGATTTGAATGACGTGT‑TGATGCCAGTATCATTAAGGGATGATAAAATTATCAAACATATGGCGCCA‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCGCCta                                                                                                                                                                                                           <  1:244207/239‑1 (MQ=255)                                            gAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTCTACCCGCa                                                                                                                                                                                                   >  2:788780/1‑238 (MQ=255)                                               cTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCTTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTAAAAAAAAGGGCTC‑GCCCCCCTGGCATACCACCAAAACCCCCaaac                                                                                                                                                                                                >  1:123056/1‑236 (MQ=255)                                                 tGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑C‑GGACCCTCAAACGGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAAACCTGGTGAAAAACAGGACTC‑CCTCCCCCGTCAGACAACCCAAAACACCAACCGcc                                                                                                                                                                                             >  1:109916/1‑239 (MQ=255)                                                   tttttATTTTTCGCCAATTTGTTATTGCCCTATCCCCAATTTAGGAACCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCaa                                                                                                                                                                                           <  2:620191/239‑1 (MQ=255)                                                            ttCCCCAATTTGTTTTTGCATTCTCCCAATTTTATAAACACAAATAAAATTGATTTAAAGGACGTTT‑GGAGGCAAGAATCATTAAGTGAAGAAAAAATAATCAAACAGATGGCCCGG‑A‑GGACCCCAAAATTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCAACAAGCCCTGGTGAAAAACAGGCCTC‑GCCCCCCTGCCAGCCCACCTATACCCGCAACCGCCAAAACGCCAGa                                                                                                                                                                                  <  1:788780/239‑1 (MQ=255)                                                                    tatGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAAAACCGCACAACGTTA‑CTCACGAAAACCTGGTGAAAAACAGGCCTA‑CACCCCCCGTCAGACCCCCCAAACACGCACCACCCAAAACGCCAAAccccccca                                                                                                                                                                          >  1:423823/1‑237 (MQ=255)                                                                      tGTTTTTGCTATATCCCCAATTTATGAATCCCAATTAAAGTGATTGGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGaa                                                                                                                                                                        <  2:960913/239‑1 (MQ=255)                                                                             gctttATCACAAATTTATGAACTCAAAGTAAAGTGTTTTGTA‑GTCGTTTGTGAGTCCAGCCATATAAGGTGATGAAAAAATTATCTAAGCAGTGGCAAGC‑A‑GGACCATAAAGCGGTTTGTAAAAAAGAAAACGCCGCCAAAAGGTA‑CCCACGTAGACCTGGTAAAAAACAGGCCTC‑GCTCCCCGGTCAGACCCCCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCAtt                                                                                                                                                                 <  1:439534/235‑1 (MQ=255)                                                                                cTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTaa                                                                                                                                                               >  2:272962/1‑238 (MQ=255)                                                                                cTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGATA‑CTCACGAAGACCTGGTGAAAAACAGGCACC‑GTGCCCCTGTAAAACCACCTATACACGCAACAGCCAAAACCACAGACCACCCTGAAcacaaataaa                                                                                                                                                              >  1:583013/1‑230 (MQ=255)                                                                                  aTCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGAccctgtcccttatccccatct                                                                                                                                                             >  2:72284/1‑219 (MQ=255)                                                                                   tccccAATTTAGAAACCCAAATGAAAATGATTTAAAAGACGTTT‑GGTGGCCAGCGTCATAATGTGAAAAAAAAAAACACAAACATGGGGGGCAC‑A‑GGACCCAAAACGTGTTTGAAAAAAAGGAAACCCCGCAAAAAGTTA‑CTCCCGAAGACCGGGGGAAAAACAGGCCTC‑GCCCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCg                                                                                                                                                           <  1:315108/236‑1 (MQ=255)                                                                                   tCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGac                                                                                                                                                                                                                 <  2:62367/185‑1 (MQ=255)                                                                                   tCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGac                                                                                                                                                                                                                 >  1:62367/1‑185 (MQ=255)                                                                                   tCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCg                                                                                                                                                           >  2:11640/1‑239 (MQ=255)                                                                                         aTTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGATA‑CTCACGAAGACCTTGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAAACCCCAAAACGCCAGACCCCCCCCAACCTCATAAAACCgcaaagt                                                                                                                                                     >  1:311505/1‑233 (MQ=255)                                                                                          tttATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCACCCGCCAAAACGCCAGACCTCCCTGAACGCCATTAAACCGTGATGt                                                                                                                                                     >  2:941770/1‑238 (MQ=255)                                                                                                            agcgATTTGTATGACGTGT‑TGATTCAAGCATCATTAAGCGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACGGTTTGTAAAAAATGAACAACAGCCAACAGATA‑CTCACAAATACATGGCGAAAAACAGGCATC‑ACCCCACTGTAATACCACACAACCCCGCAACGGACAAAACGCCGGACACCCCCGAACGCACTTAAACCACGATGTTCCCggccccctacacctt                                                                                                                                  >  1:222049/4‑225 (MQ=255)                                                                                                                aTTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGCAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTACCCGCCCCTCTGAcgcggaaaa                                                                                                                              >  1:208724/1‑234 (MQ=255)                                                                                                                     aaTGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGACATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATc                                                                                                                         >  2:954736/1‑239 (MQ=255)                                                                                                                          cGTGT‑TGCTGCCAGCATCATTAAGAGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGGCCACCTATACCCGCAACAGCCACAACGCCAGACCTCCCTGAACGTCATTAAACATTGATGTTACCGACTCTCTGACGCCTGAAAGAGTCAGCGt                                                                                                                    >  2:237197/1‑239 (MQ=255)                                                                                                                                    gCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCTTGATGTTACCAACTCTCGAACGCGTGAAAGAATCACGTTCAAAGAAAc                                                                                                           >  2:883631/1‑239 (MQ=255)                                                                                                                                      cAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGCAGCGTGAAAGAATCAGCTTCagaagagcgg                                                                                                         >  2:717387/1‑232 (MQ=255)                                                                                                                                       ggCATCATAAGGTATTGATAAAATTATCAAACATAGGGCGCGC‑A‑GGACCCTAAATCTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGCCCGGGTAAAAACCAGGCCTC‑GCTCCCCTGTCAGACCACCTATCCCCGAAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGa                                                                                                        <  2:423823/238‑1 (MQ=255)                                                                                                                                       gatatcataaaggtatataaaaaTTAACAAAAATACGGCCAGA‑A‑GCCCCCCAACAGTGTTTGAAAAAAAAGAACCACAGAAAAACGCTA‑CCAAGAAAGGCCGGGGAACAACCAGGCCCC‑CCTCCCCGATAAGACCCCCACAACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGa                                                                                                        <  1:1007349/220‑1 (MQ=255)                                                                                                                                              ttAAGTGCTGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCACTACCAGCAACCGCCAAAAACCCATACCTCCCTGAACGTCATTCAACCGTGAGGTTACCGACTCTCTGACGCCGGAAAAAATCAGCGACAGAAGAACGGCAAAcgc                                                                                                  >  1:314927/1‑238 (MQ=255)                                                                                                                                                 aGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCCAt                                                                                               >  2:789847/1‑238 (MQ=255)                                                                                                                                                      tgatAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑CCTCCCCTGTCAAACCACCTATACCCGCAAACGCCAAAACGCCAGACCTCCCTGAACCTCCATAACCCGCGATGTTACCGCCTCCCCTAACCCTGAAAGAACCACCATCACACAAACGGAAAAcaccacccaca                                                                                          >  1:424797/1‑228 (MQ=255)                                                                                                                                                      ataaaaaaATAACAAACA‑ATGGGGAGC‑AGGGACCCCTACATTGTTAGAAAAAAATAAAACTCCCGCAAAATTCA‑CTCACGAAACCTTGGTGAAAAACAGCCCTC‑CCTCCACGTACAAATCCCCTTGCCCCGCAACCGCCAAAACGCCAAACCTCACGGAACGTCATTAATCCGTGATGTTACCGACTCTCTGACGCGTGAAAGAACCAGCGTCAGAGAAACGGAAAACGCGATACAGAt                                                                                         <  1:883631/235‑1 (MQ=255)                                                                                                                                                      agaaaaaaTTA‑CAAACACATCGCGCGCGA‑GGCCCCCCAAATCGTTGGAAAAAAAAGAACCCCCGCAAAAAGTTC‑CTCCAGAAGCCCTGGTGAAAAACAGCCCTC‑GCTCCCCTGTCAGACCCCCTATACCCGTAACCGCCAAAACGCCAGCCCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGAt                                                                                         <  1:5487/235‑1 (MQ=255)                                                                                                                                                           aagaaaaagaaCATATGGCGCGC‑A‑GGACCCTAAAACGGTTTGAAAAAAAAGAAACCCCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACaa                                                                                    <  2:580441/230‑1 (MQ=255)                                                                                                                                                                        tatGGCCCGC‑A‑GGACCCACAAACTGTTTGAAAAAAATGAAACCCCGCCAAAGTTTA‑CTCCCGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCCCCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTc                                                                       <  2:109916/239‑1 (MQ=255)                                                                                                                                                                           ggCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTcaca                                                                    >  2:1050150/1‑239 (MQ=255)                                                                                                                                                                               cgc‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCCTTAAACCTGGATGTTACCGCCCCTCCGACGCGTGAAAGAACCACCGTCCGAGGAACGTACAACCCCGTCCAGAACCCAAATCCATTGTAtccggctctt                                                                >  1:580441/1‑230 (MQ=255)                                                                                                                                                                               cgc‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTAACGACCCTCTGACGCGTGAAAGAATCAGCGACAGAGAAACGGAAAACGCGATCCAGATCACAAAAgcattgcatttcaatcat                                                                >  2:463946/1‑231 (MQ=255)                                                                                                                                                                               cgc‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCCCCCCTGTCAGACCACCTATACCCGCAACCGCCAAACAGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTCCCGACCCTCTCACGCGTGAAAGAATCAGCGTCAGCGAAACGG‑AAACGCGCTCACAATAACAAACGCATGGAAttacaataaca                                                               >  1:220359/1‑230 (MQ=255)                                                                                                                                                                                    caccccccaaacgtttttcaaaaaaaTCAAAC‑CCCCCAAAAGTCACCTCACGAAGCCCGGGTGAAAACACGCCCTC‑GCACCCCTGACACCCCCCTTACACCCGCACCCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTaa                                                             <  1:1081500/219‑1 (MQ=255)                                                                                                                                                                                                tttttgaaaaaaaaGAAAACCCCGCAAAAAGATC‑CCCACGAACCCCTGGTAAAACACAGGCCCC‑TCCCCCCTGTCACACCACCAACACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGAt                                                 <  1:11640/231‑1 (MQ=255)                                                                                                                                                                                                     tGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCCTTAACCGTTTTAAGATCAttt                                            >  2:1137770/1‑239 (MQ=255)                                                                                                                                                                                                        aaaaaaGGAAACCCCCCAAAAATTTA‑CCCCAGAAGACCGGGTAAAAAACGGGCCTC‑GCCCCCCTGTCAGACCCCCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTtcat                                         <  2:208724/239‑1 (MQ=255)                                                                                                                                                                                                                      cGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATACATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAAc                           >  2:622293/1‑239 (MQ=255)                                                                                                                                                                                                                       gCCAAAAGTTA‑CCCACGAAAGCCTGGTAAAAAACAGGCCCC‑GCTCCCCTGTCAGACCACCTATCCCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACt                          <  2:314927/239‑1 (MQ=255)                                                                                                                                                                                                                       ccctaaaGTTA‑CCCAC‑AAGCACTTGGGAAAAAAAGGCCTCACCCCCCCTCCAAGACCTCCTATCCGCGCAACCGGCAAAACGCCAGACCCCGCTAAACGGCATTAAACCGGGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCAAAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACt                          <  1:1137770/235‑1 (MQ=255)                                                                                                                                                                                                                                tA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAAcc                                                           >  2:1157941/1‑197 (MQ=255)                                                                                                                                                                                                                                  acccacaAAGACAGGGTGAAACACAGGCCTC‑GCTCCCCTGTCAGACCCCCTATACCCACAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACTCACCCGATaa                <  2:674720/236‑1 (MQ=255)                                                                                                                                                                                                                                          aggccgggTAAAAACCAGGCCCC‑GCCCCCCTGTAAAACCCCCTTACCCCGAAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACTCACCCGATAATCTGTTa         <  2:424797/232‑1 (MQ=255)                                                                                                                                                                                                                                                ggTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTAAAGATAATTGCATCACTTTTTGGCAACTAACCCGATAATCTGTAATGacaca  >  2:811892/1‑237 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             GCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGT‑TGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGC‑A‑GGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCCAAAAGTTA‑CTCACGAAGACCTGGTGAAAAACAGGCCTC‑GCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACTCACCCGATAATCTGTTATGACAAC  >  NC_000913/1616562‑1617035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑