Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 175,537 T→G 21.0% V144G (GTG→GGG)  clcA → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913175,5370TG21.0% 118.7 / 3.8 56V144G (GTG→GGG) clcAH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base T (9/33);  new base G (12/2);  total (21/35)
Fisher's exact test for biased strand distribution p-value = 3.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGG  >  NC_000913/175308‑175768
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
cTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGGGGCATGGGGTTtggggc                                                                                                                                                                                                                                  >  2:535860/1‑237 (MQ=255)
 tgtgtttaatagacagtAATTATCCGCTGCGTTTAACCGTCGCTTTTCGCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGgcgc                                                                                                                                                                                                                                 <  2:913323/223‑1 (MQ=255)
      cATACGTCTTATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGTTCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAgg                                                                                                                                                                                                                            <  2:64230/239‑1 (MQ=255)
         aCTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGGGTTGGGGCGCGAAGGGcc                                                                                                                                                                                                                         >  2:916615/1‑239 (MQ=255)
         aCTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGGTCTTTGGCGGGCTGGGGACACCCGGCGGAGGCTTGGGGGTGGGGCGCGAAGGGcc                                                                                                                                                                                                                         >  2:650312/1‑239 (MQ=255)
           tgctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGGGTTGGGgctgtctctttaac                                                                                                                                                                                                                       >  2:351428/1‑227 (MQ=255)
           tgctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGGATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGAAACTCGGCGGAGGCATGGGGTTGGGGGCCGAAGGGCCa                                                                                                                                                                                                                        >  2:948769/1‑238 (MQ=255)
           tgctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGGATTGCCGGGGAAGTTTTTTGGCGGGCTGGGGACACTGGGCGGGGGCATGGTGTTgggggcggcggggccc                                                                                                                                                                                                                        >  1:848106/1‑226 (MQ=255)
           tgcagATAATTTTCCGCTTCTGGTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTGCTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCaa                                                                                                                                                                                                                       <  2:1246492/239‑1 (MQ=255)
           gtcttttttgtttCCTCTTTTTTTAACCGTTGTTTTTCTCTGGTCGGAGGTGGTGGGGGTGGTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGAGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCaa                                                                                                                                                                                                                       <  1:535860/227‑1 (MQ=255)
             ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCCGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTCCGCTGGTGGCGGGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACAATCGGAGGAGGCATGGGGTTGGGGCGCGAAGGGCCAAcc                                                                                                                                                                                                                     >  2:408845/1‑239 (MQ=255)
             ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGGGTGTATTGCCGGGGAAGGTCTTTGGGGGGCTGGGGGCACCCGGGGGGGGGATTGTGTGGGGGGGGCGAGGGGCAAcc                                                                                                                                                                                                                     >  1:1146869/1‑239 (MQ=255)
             ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGGGTTGGGGCGCGAAGGGCCAAcc                                                                                                                                                                                                                     >  2:1164853/1‑239 (MQ=255)
                attaTTGTCCGCTTCTTTTAACCGGCGATGTTCTGTTTTCGGGGGTGCGGGCGAGGTTTGGCTGCTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATGAACGTCCCGTTCGGTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGGGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTg                                                                                                                                                                                                                  <  1:857288/236‑1 (MQ=255)
                          gCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGCAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGGGGCGGGTATTGCCGGGGAAGTTCTTTGGCGGGCTTGGGACACTCGGCGGTGGCATGGTGTTGGGGGGCGAAGGGCCAACCGTGCAGGTcgcgg                                                                                                                                                                                                        >  2:40701/1‑236 (MQ=255)
                           cTTTTGGTAACCGTCGGTTTGCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTGTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGTTCCGGGAAATTGGAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATcggcgg                                                                                                                                                                                                      <  2:234975/239‑1 (MQ=255)
                            ttCTGTTAACCGTCGCTTTACTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATcggcgg                                                                                                                                                                                                      <  2:707199/238‑1 (MQ=255)
                            ttCGGTTAACCATCGCTTTTCTCTGTTCGGCGGTACTGGCGATGTTTGGCAACTTTTTGGTCCGCGAATAAGCGCAGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGCAGATCAACGCCCCGTTTGCCGGGGGCGCGAATTGCAGGCGAAGTTCTCTGGGGGGCGTGGGGAACGCGGCCGGGGCAAGATGTTGGGGGGCGGAGGGcgcaactggatcttattcagc                                                                                                                                                                                                      >  1:913323/1‑219 (MQ=255)
                                 ttAACCGTGGCTTTTCTCGGTTCGGGGGTGGTGGCGATGTTTGGCTACTTTTGGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACAt                                                                                                                                                                                                <  2:299128/239‑1 (MQ=255)
                                 ttAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACAt                                                                                                                                                                                                <  2:964998/239‑1 (MQ=255)
                                 ttAACCGTCGCTTTTCCCTGGTCGGCGGTTCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACAt                                                                                                                                                                                                <  2:225012/239‑1 (MQ=255)
                                    aCCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTgg                                                                                                                                                                                             <  2:848106/239‑1 (MQ=255)
                                      cGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACTCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTAGCCGGGGGCGGGAATTGCCGGCGAGGGTGTTTTGCGGGCGTGGGGCACCGCGCGGGGGCCGGGGGTTGGGGGGCGCAGGGCGAACGGTGCAGactcgggggggcaacggcg                                                                                                                                                                                            >  1:1180130/1‑221 (MQ=255)
                                      cGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATcggcgggtaaatttgcc                                                                                                                                                                                           >  2:979236/1‑228 (MQ=255)
                                        tCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGGCACCCGGCGGAGGCATGGGGTTGGGGCGCGCAGGGCCAACCGTGCAGACctgcggggaaactagccga                                                                                                                                                                                         >  2:947012/1‑226 (MQ=255)
                                        tCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATcggcggcatccatttgcgg                                                                                                                                                                                         >  2:676825/1‑226 (MQ=255)
                                        tCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACCCGGCGGAGGCATGGTGTTGGGGGGCGAAGGGCCAACCCGGCAGATCGGCGGTAACAATGGCCgg                                                                                                                                                                                         >  2:672451/1‑238 (MQ=255)
                                         cGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGGGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGGCGGTGAAGTTCTTTGGCGGGCTGGGGGCGACCGGCGGGGGGAAGGGGTTGGGGGGGGGAGGGCCAAACGGGCAGacaggggggaaaaatagggggg                                                                                                                                                                                         >  1:619107/1‑218 (MQ=255)
                                          gttttgtactGTGCGGTGGTGCTGGAGATGTTTGGCTATTTTTTGGTGCGAAAATACGGCACGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCTCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTTTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTAt                                                                                                                                                                                       <  2:696097/231‑1 (MQ=255)
                                               tctcTGGTCGGGGGTTCTGGCGATTTTTGGCTGTTTTTTGGTGCGAAAATACGGGTCGGAAGCAGGTGGTTCGGGGAGCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGc                                                                                                                                                                                  <  2:1256568/239‑1 (MQ=255)
                                                  tcgTTTGGGGGTGTTGGCGGTGTTTGGCTTCTTTTTGGGGGGCAAATACGGGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTg                                                                                                                                                                               <  1:979236/237‑1 (MQ=255)
                                                           ggTTCTGGCGATGGTTGGCTACTTTTTGGTGCGCCAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTc                                                                                                                                                                       <  2:866044/238‑1 (MQ=255)
                                                           ggTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGGCGGAAGCAGGTGGGTCGGGGATACCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTc                                                                                                                                                                       <  2:632188/238‑1 (MQ=255)
                                                           ggTGCTGGCGATGTTTGGCTACTTTTGGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTc                                                                                                                                                                       <  2:1146869/238‑1 (MQ=255)
                                                             tGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGGATGGGGGTTGATATTTTCCg                                                                                                                                                                     >  2:626832/1‑238 (MQ=255)
                                                               cTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGGAACCATGGCCGGATgggggtttgtgattttccccg                                                                                                                                                                  >  2:205822/1‑220 (MQ=255)
                                                                                cTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGCGGCATGGGTTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGGGAACATTGCCCGATTGGGGTTGTAATTTTTCCCCTTAAAGGTGACGacagc                                                                                                                                                  >  2:1077834/1‑234 (MQ=255)
                                                                                         gcgcAAATACGGGCCGGAAGCGTGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACg                                                                                                                                         <  2:666179/238‑1 (MQ=255)
                                                                                          cgcAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGt                                                                                                                                                                                                     <  2:64638/177‑1 (MQ=255)
                                                                                                                                    aTTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGAt                                                                                                                                                                                                             <  2:512399/128‑1 (MQ=255)
                                                                                                                                                cTGGAAGATCAACGGCCTGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTgg                                                                                 <  2:690211/238‑1 (MQ=255)
                                                                                                                                                 tGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGc                                                                                <  2:619107/238‑1 (MQ=255)
                                                                                                                                                  gtaagttccacgctctgttggttgggggCGTGTATTGGCGGGGAAGTTCTTTGGCGGGATGGGGACACTCGGGGGGGGCATGGGGGTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCgg                                                                              <  1:205822/217‑1 (MQ=255)
                                                                                                                                                        tCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTAtt                                                                         <  2:1180130/238‑1 (MQ=255)
                                                                                                                                                                 cGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGGGTTGGGGCGCGAAGGGCCAACCGGGCAGATCGGCGGGAACAATTGGCGGGTGGGGGTTGTTATTTTTCGCCCGCAAGGGGACGAGGCGCGCCGTAAGCTGCTTGCGACCAGGGGCGTCGCGGGGCGGGCGTCGGCGCTTTACCGGGCGCCTgggggggggtttgtgtttt                                                                >  1:364522/1‑226 (MQ=255)
                                                                                                                                                                                  tATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGc                                                                                                      <  2:816178/184‑1 (MQ=255)
                                                                                                                                                                                   tatGCCGGTGAAGTTCTTGGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGTGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCg                                              <  2:960298/236‑1 (MQ=255)
                                                                                                                                                                                   aTTGCGGGTTAAGTTCGTTGGCGGGCTGGGGACCCTCGGGGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCg                                              <  2:800235/238‑1 (MQ=255)
                                                                                                                                                                                           tGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAAcgcg                                                                                        <  2:92194/188‑1 (MQ=255)
                                                                                                                                                                                              aGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCta                                   <  2:785637/238‑1 (MQ=255)
                                                                                                                                                                                                 tCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACg                               <  2:1093283/239‑1 (MQ=255)
                                                                                                                                                                                                                    gctggggggggggatgggTGTGGGGGGGAGAGGGACCAACGTGCGAATCGGGGGGACCCTGGGCCGTTTGGTGGTGTACTTTTTCCGACTGAAAGTTGGCGAGGCTGGCCATGCGCTGCTGGCAACCGGTGCTGTTGCGGGGCTGGCTGCGGCCTTTAACGCTCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAAATTCGATTAAAGCg             <  1:958145/222‑1 (MQ=255)
                                                                                                                                                                                                                     cctgtgcggagtggttgtgtTGGGGCGGGGAGGGACAACGGTGAGGATCGGCGTGACGATTGGCCGTATGGGGTTTGAGTGTTTCCGGCTGAACGGGGACCAACCCCGTCATACGCTGTGGGCAACAGGTGCTGCTGCGGGGATGGCTGCGGCCGTTAACGCGCCGTTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCg             <  1:408845/222‑1 (MQ=255)
                                                                                                                                                                                                                     acTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCg             <  2:364522/238‑1 (MQ=255)
                                                                                                                                                                                                                     acTCGGCGGAGGCAGTGTGTTGGGGCGCTAAGGGCCAACCTTTCAGATCGGCGGTAACATTGGCCGTATGGTTCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCg             <  2:87624/238‑1 (MQ=255)
                                                                                                                                                                                                                       tCGGCGGAGGCATGGGGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGta          <  2:139140/239‑1 (MQ=255)
                                                                                                                                                                                                                             ggggCATGGTGTTGGGGGGAGAAGGGCCAACCGTGGAGGACGGGGATAACATGTGCCGGTTGGTGTTTGATATTTGCCGCCTGAAAGGTGACGAAGCCCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGtatttat     <  1:916615/236‑1 (MQ=255)
                                                                                                                                                                                                                                ggagtgtgtGGGGGGGGGAGGGGCCAACCGTGCGGAGGGGCGGTAACATTGGCCGTATGTTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTgg  <  1:1164853/233‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
CTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACCGGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGG  >  NC_000913/175308‑175768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: