Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,207,318 A→T 13.0% L542F (TTA→TTT yehM → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,207,3180AT13.0% 214.0 / 2.8 77L542F (TTA→TTTyehMuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (10/56);  new base T (9/2);  total (19/58)
Fisher's exact test for biased strand distribution p-value = 2.37e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

CGCACGCAATAAATTAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGTAGCCTTACTGGAGCGACGCAGTCAGGCAATTCATGCCCCGGCGCTGGATCGC  >  NC_000913/2207081‑2207543
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    
cgcacgcaATAAATTAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGAGCTGAGGAAATTAGCGGGATGCAAGACGCGCAGATATTAc                                                                                                                                                                                                                                     >  2:496529/1‑239 (MQ=255)
  cacgcaATAAATTGGAAGCCGTTATGGTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTGCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAAGCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACaa                                                                                                                                                                                                                                   <  1:1255909/239‑1 (MQ=255)
  cacgcaATAAATTAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGGTGAAATTAGCGGTGTGGAAGGCGCGCAGAAATTTCaa                                                                                                                                                                                                                                   >  2:547279/1‑239 (MQ=255)
             ttAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATg                                                                                                                                                                                                                        <  2:1206555/239‑1 (MQ=255)
               aggagccagtttggggggggggggcggAACCGGCAGTAACCCCAACTGCTTTAGCCTGGAGGCGTTTGGGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACGCTATTAATCGCCCAGGAAAAGCAATTGGCCGAAATGTGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATgcg                                                                                                                                                                                                                      <  1:832368/218‑1 (MQ=255)
                 aaGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGGTGGAATTAGCGGGATGCAAGGGGCGCAGGTATTTCAAACGACGGTATggccg                                                                                                                                                                                                                    >  2:848593/1‑235 (MQ=255)
                 aaGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGGTGAAAATAGAGGGATGTGAGAGGCGCGGAAATTTCAAACGAGGAGATGcgcg                                                                                                                                                                                                                    >  2:293537/1‑239 (MQ=255)
                 aaGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTATCGGGTTGCAAAGCGCGCAGAAATTTCAAACGACGTTTTtcgc                                                                                                                                                                                                                     >  2:713963/1‑238 (MQ=255)
                  cgccgtgaggCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTCCTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGcgcg                                                                                                                                                                                                                    <  2:924362/229‑1 (MQ=255)
                  aGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATgcgc                                                                                                                                                                                                                     <  2:253674/237‑1 (MQ=255)
                   tgctatttggtggagcgggggggAATCGGCAGTAACACCACATGGTTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACTCTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGAc                                                                                                                                                                                                                  <  1:1016307/222‑1 (MQ=255)
                    ccGTTATGGGGGACGCGGGGGGAATCGGCCGTCACACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGa                                                                                                                                                                                                                   <  1:713963/237‑1 (MQ=255)
                             tGGACGCGGGCGGAATCGGCAGTATCCCCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGGCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTatcgatcg                                                                                                                                                                                                         <  2:1212545/238‑1 (MQ=255)
                              tgggggggggggAATTGGCAGTATCACCCAATGGTGTAGCCAGGCGGCGTTAGCGGGGCTGGCGCTCCACAGTCAACAATTACTGGAGCAACTCCCACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGGTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCa                                                                                                                                                                                                       <  1:1081526/233‑1 (MQ=255)
                               gACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGGTGGAATTAAAGGTATGGAAGGCGGCCCGAAATTTAAAACGGCCTTATGCGCGACTCTCGATAGCa                                                                                                                                                                                                       >  2:1138032/1‑238 (MQ=255)
                               gACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGGAAATAAGCGGTTGCAAGGGGGCCAGAAATTTAAAAAGACGGTTTCCGCGACTATCCGTCGCa                                                                                                                                                                                                       >  2:619296/1‑238 (MQ=255)
                                 gggggggggAATAGGCAGTTTCGCCACCTTTCTGTGCCCGGCGGCGTTGGCGGGTCTGGCGTCCTGCAGTCAACAATTTCTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACg                                                                                                                                                                                                     <  1:63534/235‑1 (MQ=255)
                                    gggcggAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAACGGTATGCAAGGCGCGCAGATATTTAAAAAGACGTTTTTCGCGCCCATCAGTCGCAcacctt                                                                                                                                                                                                 >  2:1237287/1‑234 (MQ=255)
                                       tggAATCGGGCGTATCCCCAAGTGTCTGAGCCAGGGGGCGTTAGCGGGTCTGGCGTCCCTCAGGCAACAATTAATGGAACAACCCACACTATTAAGCGCCCAGGAAAAGTAATTTGCAGAAATGGGCCGGGCGCTGGAAGTGCTGTATGGCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACgctgtgg                                                                                                                                                                                               <  1:743441/237‑1 (MQ=255)
                                       ccgcaTCGGGTGTAACACCACATGTTTTAGCCAGGCGGCGTTAGCGGTTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACCCTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACgctgtgg                                                                                                                                                                                               <  2:223840/234‑1 (MQ=255)
                                             cGGCAGTTCCACCACATGTCTTGGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACCCTATTAACCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCtgtgtg                                                                                                                                                                                        <  1:848593/239‑1 (MQ=255)
                                                cAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGGAATTAGCGGGATGCAAGGCGCGCCGATATTACAAACGACGGTTTGGGGGACTATCGATCGCCAGCCGTGGCTGTGTGGAt                                                                                                                                                                                     >  2:645060/1‑239 (MQ=255)
                                                    aTCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGGTGAAATTAGCGGTCTGCAAAGCGCGCAGAAATTACAAAAGCAGGTATGCGCGACTATCGATCGCACCCCGTGGCTGGGTGGATCTaa                                                                                                                                                                                 >  2:1278938/1‑239 (MQ=255)
                                                           caTGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGa                                                                                                                                                                          <  2:795162/239‑1 (MQ=255)
                                                           caTGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGa                                                                                                                                                                          <  2:1038811/239‑1 (MQ=255)
                                                             tttttttgCCAGGCGGGGTTGGGGGGTCGTGCCTCCTTACATCAAACTTTACCGGAGGAAACTACCGTGTTAATCGCCCAGGAAAATTAATTGGCCGAAAGGGGCCAGGCGCTGGAAGTGCGTTATTCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCGGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGAcc                                                                                                                                                                        <  1:933239/232‑1 (MQ=255)
                                                             tGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCCCACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGAcc                                                                                                                                                                        <  2:784952/239‑1 (MQ=255)
                                                             tGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGGTGAAATTAGCGGGATGCAAGGCGCGCAGATATTTCAAACGAAGTTATTCGCGGCTATCGATCGCAAGCTGTGGCTGTGTGAATCTAACGGCAGAcc                                                                                                                                                                        >  2:858047/1‑239 (MQ=255)
                                                             cccggagagttgtataagaggcagggTTTGGCGTCCTTTAGTCAACAATTACCGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTAGGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGAcc                                                                                                                                                                        <  2:307353/216‑1 (MQ=255)
                                                               tCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCa                                                                                                                                                                                                       <  2:192878/206‑1 (MQ=255)
                                                                   aGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGGAATTAGCGGTATGCAAAGCGCGCAGATATTACAAACGAAGGTATGTGGGCCTATCGATTGCACGCTGTGGCTGTGTGGATCTAAAGCCAGACCCGGTGa                                                                                                                                                                  >  2:610529/1‑239 (MQ=255)
                                                                        ggcggcGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGTAGTGCTTTATGCCTTATGGCGGCCGGATGAAAATAGCGGTATGCTAGTCGCGCCGATATTACAACAGCAGCTTTGGGCTCCCATCCATCGCACGCTCGGGGTGGGTGGGTCTTACGGCaagcagcgcggaaaag                                                                                                                                                             >  1:1113119/1‑224 (MQ=255)
                                                                        ggcggcGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCCGATATTTCAAACGACGTTATGCGCGACTATCGATCGCACGCCTTGGGTGTGTGGATTTAACGGCAGACCGGAGGAAAAgg                                                                                                                                                             >  2:861475/1‑239 (MQ=255)
                                                                          cggcgTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAg                                                                                                                                                              <  2:1113119/236‑1 (MQ=255)
                                                                            gctttAGCGGGTCTTGCGTCCTGCAATCATCAATTTCTGGACAATCTCACTCTTGTAATCGCCCAGGAAAAGTAAGTTGCCGAAACGTGACCGGGGCGGAAAGTGGTTGTTCCCTTACGGCGGCTGGATGAAATTTGCGGTATGCAAGGCGCGCCGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTCGCTGTCTGAATCTAACGGCACACCGGATGAAAAGGCGtt                                                                                                                                                        <  1:256510/236‑1 (MQ=255)
                                                                            gccttcgCGGGTCTGGCCTCCTTAAGTCAACAATTACTGGAGCCACTCCCCATATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGtt                                                                                                                                                        <  1:999295/233‑1 (MQ=255)
                                                                             cgTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGtt                                                                                                                                                        <  2:860524/238‑1 (MQ=255)
                                                                                     ggTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTc                                                                                                                                                <  2:272435/238‑1 (MQ=255)
                                                                                       tCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCAc                                                                                                                                              <  2:626379/238‑1 (MQ=255)
                                                                                                          caatgcttGGAGCAATCCACACTATTAGTCCTCTATGAACATATATTTGCCGAAATGGGGCTGGCGCTGGGACTGCCTTATGCCTTATTGCGCTTGGAATAAAATAGAGGTATGCAAGGCGCGCAAATAGTACAAACGACGTGAGGCGCGACGAACGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTGACGCTCACCTGCATAGCTGGCAAGCGCt                                                                                                                          <  1:220171/232‑1 (MQ=255)
                                                                                                          caaTTTCGGGCGCAACTCACACTATTAAACGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGGGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCt                                                                                                                          <  2:1198975/239‑1 (MQ=255)
                                                                                                          caaTTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTGGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCt                                                                                                                          <  2:556999/239‑1 (MQ=255)
                                                                                                                        actcccccgttttaccGCACAGGAAAATTAATTTGCCGAAAGGGGCCAGGGGATGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAAGTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTgc                                                                                                            <  1:858047/224‑1 (MQ=255)
                                                                                                                        actacgtcttttAATCGCCGAGGAAAATCAATTTGCCGAAAGGGGGCAGGCGCGGGAAGTGCGTTATGCCTTATGGCGGGTGGATGAAATTAGCGGGATGCAAGGCGCGCAGATATTACAAACGACGGTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTgc                                                                                                            <  1:590158/229‑1 (MQ=255)
                                                                                                                        aCTCCCACTAATAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTgc                                                                                                            <  2:986883/239‑1 (MQ=255)
                                                                                                                        aCTCCCAATATTAATCGCCCAGGAAAAGCAAGTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCCTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTgc                                                                                                            <  2:898133/239‑1 (MQ=255)
                                                                                                                        aCTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTgc                                                                                                            <  2:275999/239‑1 (MQ=255)
                                                                                                                        aCTCACACTATGAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCTCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTTTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTgc                                                                                                            <  2:48022/239‑1 (MQ=255)
                                                                                                                         cTCCCACTATTAACTGCCCAGGAAAATGAATTTGCCGAAAGGGGCCAGGCGCTGGAAGGGCTTTATGCCTTATGGCGGCTGGGTGAAATTGGCGGTATGCAAGGCGAGCAGATATTTCAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTgcg                                                                                                           <  1:1256245/239‑1 (MQ=255)
                                                                                                                         cTCACCCTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCTGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTgcg                                                                                                           <  2:558672/239‑1 (MQ=255)
                                                                                                                           cccccttttAATCCCCCAGGAAAATAAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTTTGCTTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGcgcg                                                                                                         <  1:1148713/232‑1 (MQ=255)
                                                                                                                           cccccTATTAATCTCCAAGGAAAATCATTTTGCCGAAATGGGCCAGGGGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGcgcg                                                                                                         <  1:619296/235‑1 (MQ=255)
                                                                                                                           cacaCTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGAGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGcgcg                                                                                                         <  1:632821/239‑1 (MQ=255)
                                                                                                                           cacaCTAATAACCGGACAAGAAAATCAATTTGGCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGTCGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGcgcg                                                                                                         <  2:367271/239‑1 (MQ=255)
                                                                                                                             cccTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGAt                                                                                                       <  2:946158/237‑1 (MQ=255)
                                                                                                                             cacTATTAATCGCCCAGGAAAATCAAGTGGCCGAAATGGGCCGGGCGCTGGAAGTGGTTTATGCCTTATGGCGGCGGGATGAAAGTAGCGGGATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGAt                                                                                                       <  1:861475/239‑1 (MQ=255)
                                                                                                                                       gccccAGGAAAAATCCAGTTTGCGAAATGGGTCCGGGGTTGGAAGTTATTTATGCCTTATGGCGGGTTGATGTAATTAGCGGGATGCAAGGCGCTCAGATGTGACAAACGACGTTATGCGCGACTATCGATCGCCCGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATAGTCTGCGCGATCTACATAgc                                                                                              <  1:195585/237‑1 (MQ=255)
                                                                                                                                          ccAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAgcggcg                                                                                          <  2:946405/239‑1 (MQ=255)
                                                                                                                                            aGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGGAAATAGCGGGATGCAAGGCGCGCAGATATTACAAAAGACGGTTTTCGCGACTAACCATCCCACGCCGTGGCTGTGTGAATCTAACGGCCGACCAGAAGAAAAAGAGGGTTCGCCCCCCCTCACTAACTTGCAAACGCATTGCCATATTTTGTGCGACCTACaaacaggcggt                                                                                        >  1:578083/1‑229 (MQ=255)
                                                                                                                                                    tCAAGTTGCCGAAATGGGACAGGCGATGGAAGTTCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTAc                                                                                 <  2:969571/239‑1 (MQ=255)
                                                                                                                                                         ttGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGGATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAAGGGTTTCACGCTCACCTGCCTAGCTGGCAAACGCTTTGCCCTATTCTTCGCGATCTCCATAGCGGGGTTAATTTTCCCCGc                                                                            >  2:547037/1‑239 (MQ=255)
                                                                                                                                                                       cAGGCGCTGGAAGTGCTTTAAGGCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGGACAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTTAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTg                                                               <  2:861332/238‑1 (MQ=255)
                                                                                                                                                                            cgtGGAAATTCTTTTTGCCTTTTGCCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACTGTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGcggcgg                                                         <  1:645060/237‑1 (MQ=255)
                                                                                                                                                                             cttggagggtttttttcctttaggcggcTGGATGAAATGAGAGCTCTGCAAGTGTGGAAGGTATTTCAATCGACGTTATGCGGGAACTTCGATGGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATTAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGt                                                        <  1:293537/217‑1 (MQ=255)
                                                                                                                                                                             cTGGAAGTGGTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTAGGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGcggcgg                                                         <  2:989478/238‑1 (MQ=255)
                                                                                                                                                                              tggaatggttttATGCCTTTGTGCGGGTGGATGAAATTAGAGGTAAGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGt                                                        <  1:540569/229‑1 (MQ=255)
                                                                                                                                                                              tGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGGATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTTGCAAGCGCCTTGCCATCTTCTGCGCGATCTCCATATCGGCGTTAATTTTCCCGGCGGTTCTCTTTCTTCGGCCGtg                                                       >  2:540569/1‑238 (MQ=255)
                                                                                                                                                                               ggAAGTGCTTTATGCCTTATGGCGGGTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGTAg                                                      <  2:36256/239‑1 (MQ=255)
                                                                                                                                                                                      cTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGcggcgg                                                         <  2:1189658/229‑1 (MQ=255)
                                                                                                                                                                                             ccTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGcgcg                                                                                                         <  2:811656/174‑1 (MQ=255)
                                                                                                                                                                                              cTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGTAGCCTTACTGGAGCGa                                       <  2:782169/238‑1 (MQ=255)
                                                                                                                                                                                                         cTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGTAGCCTTACTGGAGCGACGCAGTCAgg                             <  2:575965/238‑1 (MQ=255)
                                                                                                                                                                                                                     tAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGAGCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGTATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGTAGCCTTACTGGAGCGACGCAGTCAGGCAATTCATGccc                <  2:689778/239‑1 (MQ=255)
                                                                                                                                                                                                                     tAGCGGTATGCAAGGCGCGCAGTATATACAAACGTCGTTATGCGCGAGTATCGATGGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCCGCGGCGGTAGCCTTACTGGAGCGACGCAGTCAGGCAATTCATGccc                <  2:1250824/239‑1 (MQ=255)
                                                                                                                                                                                                                      aGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGt                                                        <  2:645503/200‑1 (MQ=255)
                                                                                                                                                                                                                            aTGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGTAGCCTTACTGGGGGGAACCAAGCCGGGAATTCATTCCCCCgcgcc         >  2:582634/1‑238 (MQ=255)
                                                                                                                                                                                                                                     gcgcAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGTAGCCCTATCTGGGCGAGCCACACAGGCAAATTCATGCCCggggccgggtcccg  >  2:650186/1‑227 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    
CGCACGCAATAAATTAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCCTTCAGTCAACAATTACTGGAGCAACTCACACTATTAATCGCCCAGGAAAATCAATTTGCCGAAATGGGCCAGGCGCTGGAAGTGCTTTATGCCTTATGGCGGCTGGATGAAATTAGCGGTATGCAAGGCGCGCAGATATTACAAACGACGTTATGCGCGACTATCGATCGCACGCTGTGGCTGTGTGAATCTAACGGCAGACCGGATGAAAAGGAGTTTCACGCTCACCTGCATAGCTGGCAAGCGCTTTGCCATATTCTGCGCGATCTACATAGCGGCGTTAATTTACCCGGCGTTTCTCTTTCTGCGGCGGTAGCCTTACTGGAGCGACGCAGTCAGGCAATTCATGCCCCGGCGCTGGATCGC  >  NC_000913/2207081‑2207543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: