Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,274,347 T→G 29.8% G57G (GGT→GGG yejB → microcin C ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,3470TG29.8% 91.6 / 18.1 73G57G (GGT→GGGyejBmicrocin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (12/37);  new base G (21/3);  total (33/40)
Fisher's exact test for biased strand distribution p-value = 5.62e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGT  >  NC_000913/2274111‑2274580
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
gATACCGGGTGGTATGACGGCAATAAAGCGGCCAACCTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGgtgt                                                                                                                                                                                                                                           <  2:1232426/239‑1 (MQ=255)
 ttcccTGGTGGTTTGACGTCAATAAAGCGGCCAAACGGCCGTCCGCCAGCAAAAAGGGAGAGTAGATGGGCGTTTACCTGATTCCCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGtt                                                                                                                                                                                                                                          <  1:334957/236‑1 (MQ=255)
 ctccttggtggTAACCCGACTATAAGGCGGCAAAACTGCCTGCCGCCGGCAAACGGGGAGAGTAGATGGGTGCTTCCCGGATTCGCCTTGTGTTGCTGGGGATCCCAACATTAGGGGGGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGtt                                                                                                                                                                                                                                          <  1:422122/234‑1 (MQ=255)
      tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCCGGCGGGGAAGGGGtttggt                                                                                                                                                                                                                                      >  2:1169391/1‑234 (MQ=255)
            tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGTTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGCGCAGGTGTTccggacgccca                                                                                                                                                                                                                               >  2:460102/1‑229 (MQ=255)
            tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGTgccagc                                                                                                                                                                                                                                 >  2:1020012/1‑237 (MQ=255)
            tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGCGGGGGCAGGGGTTACTgcccgcc                                                                                                                                                                                                                                >  2:209327/1‑238 (MQ=255)
            tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGCCGACAAGGCCATCGCCGCCATTGAGGTTGCTAATGCCGGGGGATTACCCGGCACAGGCGGGGGAGGGGTTCGTgccgtcc                                                                                                                                                                                                                                >  1:580067/1‑234 (MQ=255)
             ttgtgagaaatacacggcccAACCTGCCCGGCGTCAGCAAAAAGGGGGAGGGGATGGGTGCTTACCTGATCCGCCGTTCGTTGTTGGCTGTCCAAATATTCTGGGCTATTAGCACCATCAAATTTTACAGCGGTAAAATTGCGCCGGGCGGTCTGCTCGACCAGGCCTGCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTgccagcca                                                                                                                                                                                                                               <  1:74938/220‑1 (MQ=255)
             aTGGCGTCAAAAAAGCGCACAAGCGCCCTGCCGCCGGAAAAGAGGGAGAGTCAATGGGCGCTTCCCTGATTCCCCTTCTGTTGCGGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCACCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCAt                                                                                                                                                                                                                              <  1:946722/239‑1 (MQ=255)
                 cGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGCGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATAGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGTGCCAGCCAtcggc                                                                                                                                                                                                                          >  2:45371/1‑235 (MQ=255)
                  gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTTAAGGGGTTCGTGCCAGCCATGCGCa                                                                                                                                                                                                                         >  2:584185/1‑239 (MQ=255)
                      aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCCAACg                                                                                                                                                                                                                     >  2:1169437/1‑239 (MQ=255)
                          aggggCCAAACTGCGGGCCGGCAGCAAACAGGGAGGTTAAATGGGCGTTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGgtg                                                                                                                                                                                                                 <  2:94425/236‑1 (MQ=255)
                          aGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGTgttgtctcttttaaccacttcc                                                                                                                                                                                                                 >  2:735514/1‑218 (MQ=255)
                            cGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGCCGGTGAAGGGGTTCGTGGCGGCCATGGCCAAACGGgggtg                                                                                                                                                                                                               >  2:1245350/1‑238 (MQ=255)
                            cGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGACCGGTCGACAAGGCCTCGTCCCCCATGGAGTTTGTTAATGCCGGGGCATACCCCGGGCCAGCCGGTGAAGAGGTTCGGaccaaccaaacagaaagggagggg                                                                                                                                                                                                               >  1:582549/1‑223 (MQ=255)
                            cGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCGGGCGGTCCGGTCGACCAGGCCATCCCCGCCATTGGTTTTGGTAGTGCCGGAGTATTACCCGGCGCAGGGGGTGAAAGGGGTCGTTCAACCCATGGGAAAACggagggc                                                                                                                                                                                                               >  1:1104920/1‑234 (MQ=255)
                                         gTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCCGCCATGCGCAAACGGGGGTCgcgcaaataagc                                                                                                                                                                                                   >  2:829707/1‑227 (MQ=255)
                                            agCCAGCAAAAAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                                <  2:752061/237‑1 (MQ=255)
                                                             agTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGGATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCCAACGGGGGTCGGCCATATCAGCGACAGCAATTACCCGGGCgg                                                                                                                                                                             >  2:661967/1‑238 (MQ=255)
                                                                aGATGGGCTCTTCCCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                          <  2:1264007/238‑1 (MQ=255)
                                                                       cgcTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGGGACAAGAATTACCGtccgtctcttataaaca                                                                                                                                                                  >  2:1277659/1‑223 (MQ=255)
                                                                         cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGa                                                                                                                                                                 >  2:1059464/1‑238 (MQ=255)
                                                                         cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGGGCCAACGGGGTTCGGCAATATCAGCGACAGGAATTACCCGGGCGGACCCGGAtttaa                                                                                                                                                                 >  2:1177468/1‑235 (MQ=255)
                                                                         cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGTTTTGTGCCAGCCATGCGCAAACGGGGTTCGGCAACATCAGCGCCCGTAATTAcccggggggtccgcggttgca                                                                                                                                                                 >  2:30111/1‑219 (MQ=255)
                                                                          tttccTGATTCGCCGTCTGTGGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTTCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATc                                                                                                                                                               <  2:1150425/236‑1 (MQ=255)
                                                                            aCCAGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCa                                                                                                                                                             <  2:242590/239‑1 (MQ=255)
                                                                                             ttGCTGGTGATCCCAACATTATGGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCt                                                                                                                                                 <  2:662047/234‑1 (MQ=255)
                                                                                                 ggggatcccacaacttgtGGGCCGTTATCCCCTTCAACTTTTTCCTCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCAGCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTATCGTGGCGGACGCGGATTAGATCCAGAAGGGATCGCTGAGATCACt                                                                                                                                        <  1:1020012/222‑1 (MQ=255)
                                                                                                  ggTGATCCCAACATTAGGGGCGATTAACACCATCAACTTTTTCATCATGCAAATTGCGCCTGGCGGTTCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACt                                                                                                                                        <  2:1296612/238‑1 (MQ=255)
                                                                                                   gTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCCTCGCCGCCATTGAGTTTGGTAATGCCGGAGCATTACCCGGCGCAGGCGGCGACGGTGGTCGTGCCAGCCATGAGCAAAACGGGGTCGGGACTATCAACGACAGTAATATACCCGGCGGACGCGGGGCAGAACCCAAAGTGATCCCGgaggagacta                                                                                                                                       >  1:1155148/1‑231 (MQ=255)
                                                                                                    tgtcccaactgttATGGGCGATTATCACATACCAATTTTTCATCGTGCAAAGTGCGCCGGGCGGCCGGGTCGACCGGGCCATCGCCGCCAGTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTTCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGTCAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCAt                                                                                                                                     <  1:19175/228‑1 (MQ=255)
                                                                                                       tCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGGGCCAGCCCAGCGCAAACGGGGGGCGGCAATATCAGCGGCCGTAATTAACCGGGGGGGCCGGGGTTATATCCAAAAAGGATCGGCGAAATCACTCaccat                                                                                                                                  >  1:662047/1‑235 (MQ=255)
                                                                                                         ccAACATTTTGGGGTATTACCCCCATCAACTTTTTCAGGTTGAAAATGGCGCCTGGGGGCCCGGTCGCCCAGGCCTTGGCCGCCATTGAGTTTGTTAATGCCGGAGTATTTCCCGGCGCAGGCGGTGAAGGTGTTCGTGCAAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTa                                                                                                                                <  1:1245350/239‑1 (MQ=255)
                                                                                                         caaacttgtggggggttttcctccAACAATTTTTCCGTCGTAAAAATTGGGCCGGGCGGTCCGGGCGACCGGGCCAGCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCCGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTa                                                                                                                                <  1:1177468/217‑1 (MQ=255)
                                                                                                          cAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTTGTGCCAGCCATGCGGAAACCGTGGGCGGCACTTACAGCGGCCGTAATTACCGTGGGGGACGCGGGTTAGATACAGAAGGGGGCGCTgcgagaccccctcccccc                                                                                                                               >  1:298288/1‑225 (MQ=255)
                                                                                                                  tGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACCGTAATTACCGTGGCGGACGCGGGTTAGATCCCGAAGTGATCCCTGAGATCACCCACCGCTACGGGTTTgt                                                                                                                       >  2:1198639/1‑238 (MQ=255)
                                                                                                                  tGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGGGCCAGCCAGTGGCAAACGGGGTCCGGCAAAATCACGGAAAGTAATTACCCTGGCGGACGCGGGTTGGATCCCGAAGTGATCGCGTGGATCAccaaacgccaggggttgga                                                                                                                       >  1:351342/1‑221 (MQ=255)
                                                                                                                   gggcggttaagcccctacaCTTTTTAAGCGTGACAATTGGGCCGGGGGGTGCGGTCGCACAGGCCATGGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGAt                                                                                                                      <  1:1169437/221‑1 (MQ=255)
                                                                                                                    ggCCATTACCACCATCAACTTTTTCATCGGGCAAAGTGCGCCGGGGGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGAt                                                                                                                      <  2:481173/238‑1 (MQ=255)
                                                                                                                        aTTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGGTTAGATCCAGAAGGGATCGCTTGGATCACTCATCGCCCCGGGTTTGGTAAAcc                                                                                                                 >  2:546968/1‑239 (MQ=255)
                                                                                                                         ttactcccATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCg                                                                                                                <  2:1104920/233‑1 (MQ=255)
                                                                                                                          tATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGa                                                                                                               <  2:1024348/239‑1 (MQ=255)
                                                                                                                                taTCAATTTTTTCATGTTGCAAATTGCGCCTGGGGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGTGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACg                                                                                                         <  2:319790/238‑1 (MQ=255)
                                                                                                                                cATCAATTTTTTCATCGTGCAAATTGCGCCGGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACg                                                                                                         <  2:478103/239‑1 (MQ=255)
                                                                                                                                         tttCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGGGATCGCTGAGATAACTCATCGCTACGGGTTTGATAAGCCGATCCACGCACGTTACt                                                                                                >  2:879276/1‑239 (MQ=255)
                                                                                                                                               tctGCACATTGTGCGCTGGCTGCCCGCTGGCCCAGGCTACGGCCTCCATTGGTTTCGGTAACGCAGGAGTTTTGCAGGGCGCCGGCGGTGACGGTGTTCGTGCCACCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTATCGTGGCGGACGAGGATTAGATCAAGAAGTGCACGCTGAGATCACTAATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTATGTCaaa                                                                                           <  1:1246216/237‑1 (MQ=255)
                                                                                                                                                 tGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCACACCATGCGCAAACGGGTGGCGGCCATACAAGCGACAGTAATTACAGCGGCGGACGCGGGGTAGATCCCGAAGGGGTCGCGGAGGAGACTACACCCTACGGTGTTGATACCGCCATCCACCGACGTTCACTCAAAAt                                                                                         >  1:4266/1‑238 (MQ=255)
                                                                                                                                                                     tccggtcGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCt                                                                                                                                 <  1:396903/179‑1 (MQ=255)
                                                                                                                                                                     tccggtcGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCt                                                                                                                                 >  2:396903/1‑179 (MQ=255)
                                                                                                                                                                      ccggtcGACCAGGGCATCGCCGCCATTGGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTCCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCt                                                                     <  2:1222713/238‑1 (MQ=255)
                                                                                                                                                                         gtcGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTTGATCCAGAAGTGATCGCTGCGATCACTCATCGCTACGGGTTTGTTAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCCCTTTGa                                                                 >  2:744955/1‑239 (MQ=255)
                                                                                                                                                                            gACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGAtt                                                               <  2:574287/238‑1 (MQ=255)
                                                                                                                                                                                 cgCCTTCCCCGCTTTTTAGTTTTTTTATGCCGGGGTTTTACCCGGGGGCGGCCGTGAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCg                                                         <  1:584185/238‑1 (MQ=255)
                                                                                                                                                                                   ccATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGa                                                        <  2:351342/238‑1 (MQ=255)
                                                                                                                                                                                    tcctgggcgccATTGGGTTTTGGAAGGCCGGAGTATTACCCGGCGCGGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGGCGGCAATATCATCGACAGTAATTTCCGGGGCGGAAGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGa                                                        <  1:1198639/231‑1 (MQ=255)
                                                                                                                                                                                      tCGCCGGCAGTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGc                                                    <  2:290063/239‑1 (MQ=255)
                                                                                                                                                                                             ccATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGGGATCGCTGGGATCACTCCTCGCTACGGTTTTGGTAAGCCGATCCACGGACGTTACCTCCAAATGCTCTGGGACTACATCCGCTTTGATTTTTGCGATAGCCTGtt                                               >  2:381433/1‑238 (MQ=255)
                                                                                                                                                                                                      tttggattGCCGGTGTATTTCCCGTCGCGTGGGTGTAAGGTTTGTGTGGCAGCCATGCGCAAACGGGTGTCGGCAATATCAACTACAGTAAGTGCCGTGGCGGAAGAGGATCAGATCCTGAAGTTATATGTGAGATCTTTCATGGCTACTGTTTTGATAAGCCGAGCCACGAACGTTACTTCAAAATGCTCTGGGACTACGTCCGCTTTGATTTTGGCGATGGCTTGTTTCGCGGCGcc                                     <  1:460102/232‑1 (MQ=255)
                                                                                                                                                                                                       gtgtaatccggtgtttttACCCGGGGCAGGGGGGGAAGGTGTTCGGGCCAGCCAGGCGAAACCGGTTTTCGGCAATAGAAGCGACAGTAATTACCGTGGCGGACGCGGATTAGACCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATTCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                                    <  1:1099325/223‑1 (MQ=255)
                                                                                                                                                                                                        ggTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCCAAAAGCTCTGGGCATACAACCGCTTTGATTTTGGCGATAACCTGTTTCGCAACGCCTc                                   >  2:540335/1‑239 (MQ=255)
                                                                                                                                                                                                             tGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGGTTAGATCCCGAAGTGATCGCCGAGATCACTCATCCCCACGGGTTTGATAACCAGAACCCCGAAAGTTTATTCAAAAATCTCTTGGACTCAAACCCCCTTTATTTTGGGGCTAGccctttttcaccagccccgggtc                              >  1:100675/1‑218 (MQ=255)
                                                                                                                                                                                                                    tattttcccGGGGCAGGCTGTCACGGGTTTAGGGCCAGCCCTTGACAAACGGGTGCCTGCAATAGCAGCGACAGGAATGTCCGTGGCGGACTGGTATTAGAGCCAGAAGTGATCGCTGAGATCACTCATCGGTACGGTTTTGATAAGCCTATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  1:1110136/232‑1 (MQ=255)
                                                                                                                                                                                                                    gTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  2:305940/238‑1 (MQ=255)
                                                                                                                                                                                                                    gTATTACCCGGCGCAGGCGGTGAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTAGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  2:709411/238‑1 (MQ=255)
                                                                                                                                                                                                                    aaaTTACCCGGGGCGGGCCGTGAAGGTGATCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  2:365042/236‑1 (MQ=255)
                                                                                                                                                                                                                     tATTCCCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  2:1220779/237‑1 (MQ=255)
                                                                                                                                                                                                                     tATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGct                                                                                      <  2:1013785/175‑1 (MQ=255)
                                                                                                                                                                                                                           ccGGCTCAGGCGGTGAAGGTGGTCGGGCCCGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGCAGCGGATTAGATCCAGAAGTTATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTaaa                <  1:1059464/239‑1 (MQ=255)
                                                                                                                                                                                                                               cgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCCGCGGCAGTAATTACCGGGGCGGACGCGGGTTAGATCCAGAAGTGATCCGTGGGATCACTCCTAGCCACGGTTTTGATAAGCCGAACCACGAAAGTTACTTCAAAAAGCCCTCGGAGCACATACGCTTTGATTTTTGGGGTAACCTGTTTCGCCACGCCCCGGGGCGGACGcgcgtgaaaaaca            >  1:1220779/1‑227 (MQ=255)
                                                                                                                                                                                                                               cgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGGCAGGAATTACCGGGGGGGACGCGGCTTTGATCCACAAGTGATCGCTGGGAACACTCCTCGCGCACGTTTTGGTAAAGCGCACCCCGAACGTTACTTCCAAAAGCCCCGGGCGCACATCCGCTTTGATTTTTGCGATAGCCCTTTTCTCAGCGCCCCGGGGCGCACGCCGTTTTAAAACa            >  1:10282/1‑239 (MQ=255)
                                                                                                                                                                                                                                   cggggggagaGGGGTTCGGGCGACACATTCGCAACCGGGGGTGGGCAATATAAGCGACAGTAATTACCGGGGGGGAGGCGGATGAGATCCAGAAGTGATCGGTGAGACCACACATCGCTACGGTTTTGATAAGCCGATCCACGAACGGTACTTGAAAATGCTCTGGGACTACATCCGATTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGAAAGt          <  1:1169391/229‑1 (MQ=255)
                                                                                                                                                                                                                                           aaGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGt  <  2:1155148/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
GATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGT  >  NC_000913/2274111‑2274580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: