Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,745,095	G→C	12.8%	G81G (GGC→GGG)	trmD ←	tRNA m(1)G37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,745,095	0	G	C	12.8%	205.8 / 5.8	89	G81G (GGC→GGG)	trmD	tRNA m(1)G37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (59/16);  new base C (2/10);  total (62/27)
Fisher's exact test for biased strand distribution p-value = 5.75e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

GTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTAATTATGCCAATC  >  NC_000913/2744860‑2745332                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 gTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCTTCCCTGTGGGGCCAGATAAATCACCTTT‑CC‑GCCt                                                                                                                                                                                                                                               >  2:62404/1‑239 (MQ=255)       ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCTTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGAGTCCCTGTGGTGACAGAAAAATCACCTTT‑TC‑GCCTTC‑Accc                                                                                                                                                                                                                                        >  1:626715/1‑239 (MQ=255)       ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTATCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCCCTGACGCCCGATTGATCAAGCTTCCGTCCTTGTGGGGCCAGCTAATTCACCTTT‑GC‑CCCTTc‑ccc                                                                                                                                                                                                                                         >  2:320721/1‑238 (MQ=255)       ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCACTTTTT‑GATTCGTGGCCAGTTCGCTGACGCCCGCTTGATCAACCTTGCGCCCCTGTGGTGACAGATAAATCACCTTG‑CG‑GCCTTc‑ccc                                                                                                                                                                                                                                         >  2:1262125/1‑238 (MQ=255)       ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑Acc                                                                                                                                                                                                                                         >  2:932704/1‑238 (MQ=255)       ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACCCCCGCTTGATCAACCTTGCTTCCCCTTGGGGAAAGATAAATACCCTTT‑GG‑CCCttaccca                                                                                                                                                                                                                                         >  1:343596/1‑234 (MQ=255)           aaTAAACCGGGAAACGGAGTAAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGACCGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACccgcc                                                                                                                                                                                                                                     <  2:350157/238‑1 (MQ=255)                     gAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGACAGTTCGCTGACCCCCGCTTGATCAACCTTCGGTCCCCTTGGGGCCAACTAAATAAACTTT‑GC‑GCCTTCAACCCACCG‑GGCTTTtgg                                                                                                                                                                                                                          >  1:1081214/1‑238 (MQ=255)                      aaacggggttaatctcgttctttGCTTGAAACTCCCCCTCACGGAGAACTGAACGGCCAATTGACCATTCTTCGTCAATTTCGGTGTGAATGCCGTCC‑TCTTGTTTACTCTCGTAGCCGCCGCACCCAAGAACCAATTTTT‑GATTCGTTGCCATTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGCCAGAAAAATCACCTTT‑GC‑CCATTA‑ACCCGCCGCGGCTGTtgc                                                                                                                                                                                                                          <  1:294127/217‑1 (MQ=255)                                      cGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTcc                                                                                                                                                                                                         >  2:868033/1‑238 (MQ=255)                                        tctttgttgGTAACTCACCCCCACTGGACACGTAATCGCCGAGTGTCAACTCTTCGCAAATTCCGGTTTGGCTCACGCGT‑TCAGTGTTCTCTTCGTAGCGACCGCCCACCAGAAACAATTTTT‑GATGCGTTGCCGTTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGAAAGATAAATCACCTTT‑GC‑GCCTTC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGc                                                                                                                                                                                                      <  1:192330/236‑1 (MQ=255)                                        tCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGCATGGCGTCCCGc                                                                                                                                                                                                      >  2:777651/1‑239 (MQ=255)                                               tGGTAACTCCCCACCACTGAGAACGTAACCGCCGATTGACCATTCTTCGTCAATTTCGTTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGtt                                                                                                                                                                                              <  2:609926/239‑1 (MQ=255)                                                 gttactcccccccccgcagactgTAATCTCCGATTTCCCTTTCTTCGTCAATTTCGGTTTGAATCACGCCC‑TCATCTATCCCTTCGGAGCGACCGCCCACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGCCCCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGc                                                                                                                                                                                            <  1:214493/217‑1 (MQ=255)                                                 gTAACTCACCACCACTGAGAACGTAATCGACGATTGAACATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTAGTAGCGACCGCACACCAGCATCAATTTTT‑GATTCGTTGCCAGTTCGCTGCCGCCCGCTTGATCAAGCTTGCGCCCCTGTGGTGAACGGTAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCCCGCCCTTTCCTGCA‑TAAAAGGCGCCCCGC‑AAGGGtatc                                                                                                                                                                                            >  1:751654/1‑236 (MQ=255)                                                   taccctccccccttaagaACGTACTCGTCTATTTCCATTTCTTCGACAATTTTGGTTGGGATCACGCGT‑TCCTCTATCCCTTCGTAGCGACCGCCCACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGTTGACGCCCGTTTGATCAAGCTTGGTTCCTTGTGGTGCCAGATAAATCCCCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCAc                                                                                                                                                                                          <  1:1252279/224‑1 (MQ=255)                                                   aaCTCCCCCCCCCTGGAAACGTAATCGCCGATTGCCCATTCTTCGTCAATTTCGGTTTGGATCCCGCGC‑TCATCTATCCCTTCGTAGCGACCGACCACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCa                                                                                                                                                                                           <  2:190316/238‑1 (MQ=255)                                                   aaCTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATCCCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCa                                                                                                                                                                                           <  2:1085961/238‑1 (MQ=255)                                                   aaCCCACCACCACGGAAAACGTAACCGCCGTTTGACCATTCGTCGTCAATTTCGGTTTGGATCCCGCGC‑TCATCTATCCCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCa                                                                                                                                                                                           <  2:626715/238‑1 (MQ=255)                                                      tCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCAccct                                                                                                                                                                                       >  2:1045226/1‑237 (MQ=255)                                                        accaccacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGAC‑TGAATGGCGTCCCGC‑AAGGGTTGCACcatca                                                                                                                                                                                     >  2:343777/1‑239 (MQ=255)                                                        accaccacTGAGAACGGAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGTTCAAGCTTGCTTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCT‑TGAATCGCGTCCCGC‑AAGGCTTGCCCcctca                                                                                                                                                                                     >  2:400440/1‑239 (MQ=255)                                                                  agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑CCCCGCCCAGGCTTTTTCTGAA‑TGAAAGGCCTCCC‑C‑ACAGGGTGCACCCTCACTaaaaatcc                                                                                                                                                                           >  1:1132144/1‑232 (MQ=255)                                                                    aaCGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAATTCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTGCCGC‑AAGGGTTGCACCATCATTAACATCCCCg                                                                                                                                                                         >  2:1134722/1‑239 (MQ=255)                                                                    aaCGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGCCGTCCCGC‑AAGGGTTGCACCATCATTAACATcccc                                                                                                                                                                          >  2:53448/1‑238 (MQ=255)                                                                       gTAATCGCCGATTGACCATTCTTCGTCATTTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACCCCCGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGCCCGATAAATCACCTTT‑GC‑GCCTTC‑CCCCCCCCCGGCTTTTGCTGCA‑TGCATGCCATACCGC‑CAGGGATGCACCATCATTAACATCCACggg                                                                                                                                                                       >  2:111193/1‑237 (MQ=255)                                                                       gTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGCTAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGAA‑TGAATGGCGTCCCGC‑ACGGGTTGCACCTTCCTTAAAATCCCCGGt                                                                                                                                                                       >  2:1283622/1‑238 (MQ=255)                                                                       gTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑ACGGGTTGCACCATCATTAACATCCCCGGt                                                                                                                                                                       >  2:690516/1‑238 (MQ=255)                                                                                           cttcGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGGTGCACCATCATTAACATCCCCGGTCCGCCGCCGTCAGGCCGATc                                                                                                                                                   >  2:354550/1‑238 (MQ=255)                                                                                              cGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTTTTGTGAAAGATCAATCCACTGT‑GC‑GCCTTC‑CCCCGCCGCGGCTTTTGCGGCA‑TGGATGCAGTCCCGC‑AAGGGTTTCACCACAATTAACAACCCCGCTCCCCCCCCCCAACGACGTTCgga                                                                                                                                                >  1:1267364/1‑236 (MQ=255)                                                                                              cGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGCCAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGGTGCACCATCATTACCATCCCCGGCCCCCGCCCGTAAGGACGATCgcc                                                                                                                                                >  2:705040/1‑236 (MQ=255)                                                                                               gcaaaTTTGGGTTTGGATCCCGCCC‑TCATTTATCCCTTCGGAGCGACCGCACACAAAAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGCCCCCCGCTTAATCAAGCTTGCGCCCCTGTGGTGACAGATAAATCCCCTTG‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGATGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCa                                                                                                                                              <  2:343596/236‑1 (MQ=255)                                                                                               gTCATTGTCGTTTTGGATC‑CGCGCGTCATTAATCCCTTCGTACCCCCCGCACACCAAAATTAATTTTT‑GATTCGTTCCCATTTCGCTGCCGCCCGTTTGATCAAGCTTGCGTCCTTGTGGTGACAAATAAATCCCCTTT‑GC‑CCCTTC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCa                                                                                                                                              <  2:1204467/239‑1 (MQ=255)                                                                                                    tttCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGCTAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCCCGGCTTTTGCTGCA‑TGAATGCCGTCCCCC‑AAGGGTTGCACCATCACTACCATCCCCGGTCCGCCGCCGCAAGGCGGATCGCCCacgatc                                                                                                                                         >  1:582062/1‑236 (MQ=255)                                                                                                    tttCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCacggt                                                                                                                                          >  2:286042/1‑238 (MQ=255)                                                                                                                          cATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTAATGCGTGAAg                                                                                                                    >  2:680385/1‑239 (MQ=255)                                                                                                                          cATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGACCGTCCACGGTACGGTGCCGGCCATGCggcaa                                                                                                                     >  2:510835/1‑234 (MQ=255)                                                                                                                                   cTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGCTCGTCCACGGTACGGTGCCGGTCATGCGGGAAGTCGCGAgg                                                                                                            >  2:911176/1‑238 (MQ=255)                                                                                                                                   cTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAAAGGAGTCCCGC‑CAGGGTTGCACCACCATTAAAATCCCCGTCCCCCCGCCGACAGGAGGACCGGCCCCGGACCGGTCCCGGCCAtcgggaaatgcggggggc                                                                                                           >  1:1173810/1‑222 (MQ=255)                                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCacggtacg                                                                                                                                       >  2:991339/1‑209 (MQ=255)                                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGCggcc                                                                                                          >  2:684369/1‑236 (MQ=255)                                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGAc                                                                                                          >  2:90268/1‑238 (MQ=255)                                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGAc                                                                                                          >  2:539377/1‑238 (MQ=255)                                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGAc                                                                                                          >  2:283832/1‑238 (MQ=255)                                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGGGAAGGCGCGAGGAc                                                                                                          >  2:1064947/1‑238 (MQ=255)                                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGAGCCGGTCATGCGTGACATCGCCAGGAc                                                                                                          >  2:967199/1‑238 (MQ=255)                                                                                                                                      cGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACt                                                                                                         >  2:667287/1‑238 (MQ=255)                                                                                                                                       gTAGCGACCGCACACCAGAATCACTTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGCCGTCCCGC‑AAGGGTTGCACCCTCATTAACATCCCCGGTCCACCGCCGTACGGACGACCGTCCACGGTCCGAGGCCGGTCATGCCGGAAGTCACGCGGACTcc                                                                                                       >  2:594000/1‑239 (MQ=255)                                                                                                                                       gTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑CAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTACGGACGATCGTCCACGGTAAGGGGCCGGCCATGCGTGAGATCGAGAGGAcccc                                                                                                       >  1:758920/1‑239 (MQ=255)                                                                                                                                       gTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCTTCCACGGTACGGTGCCCGCCATGCGGAAAGGCGGAAGGACGcc                                                                                                       >  1:493855/1‑239 (MQ=255)                                                                                                                                       gTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGCGGACTc                                                                                                        >  2:1006986/1‑238 (MQ=255)                                                                                                                                       gTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGGGCCGGTCATGCGTGAAGTCGCGAGGACTcc                                                                                                       >  2:1045859/1‑239 (MQ=255)                                                                                                                                       gTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACACCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTc                                                                                                        >  2:1094596/1‑238 (MQ=255)                                                                                                                                         ggccccccccaccaaaaaTAAATTTTT‑GTTTCGTTGCCAGTTCGTTGACGCCCGTTTGATCAAGCTGGCCTCCCTGTGGTGACAGATAAACCCCTTTT‑GC‑GCCTTC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAg                                                                                                     <  2:346099/223‑1 (MQ=255)                                                                                                                                          gCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCCTTACCATCCCCGGTCCGCCGCCGTAAGGACGGTCGTCCACGGTACGGTGCCGGTCATCCGGGAAGGCCGGAGGGCCCCAg                                                                                                     >  1:346099/1‑238 (MQ=255)                                                                                                                                          gCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTAACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCAGC‑AAGGGTTGCACCACCATTAACTTCCCCGGTCCCACGCCCTAATGCCGATCGTCCACGATACGGTGCCGCTCATTCGTACACACACGGGGACTCCacc                                                                                                    >  1:464210/1‑237 (MQ=255)                                                                                                                                             aCCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑TC‑GCCTTC‑ACCCGCCCCGCCTTTTCTTGCA‑TGAATGCGGCCCCCA‑AAGGGTTCCCCCTCCATT‑ACATCCCCCGTCCCCCCCCCTAAAGACGCTCGCCCCCGGTACGGGGCCGGGCACCCGGGAAggcccgggggccccccccccc                                                                                                >  1:667739/1‑219 (MQ=255)                                                                                                                                             aCCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCg                                                                                                                                                                                                            >  2:1280642/1‑133 (MQ=255)                                                                                                                                             aCCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCg                                                                                                                                                                                                            <  1:1280642/133‑1 (MQ=255)                                                                                                                                             aCCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGc                                                                                                    >  2:828771/1‑236 (MQ=255)                                                                                                                                             aCCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCCCAGGGACTCCAGc                                                                                                    >  2:13834/1‑236 (MQ=255)                                                                                                                                             aCCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGCCGCGAGGACTCCacc                                                                                                    >  2:238018/1‑234 (MQ=255)                                                                                                                                             aCCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGGCCCGGGCATGCGTGCAGTCGCGAGGACCCCacc                                                                                                    >  1:770830/1‑234 (MQ=255)                                                                                                                                                            cAATTTTTGGTTTTGTTCCAAGTTCCTTGACGCCGTCTTGATCAACCTTGCTTCCTTGTGTTAAAAAAAAAATCCCCTTT‑GC‑CCCTTC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAgg                                                                                   <  2:1081214/239‑1 (MQ=255)                                                                                                                                                                tttt‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGACCACCGGGCCAtt                                                                              >  2:131570/1‑239 (MQ=255)                                                                                                                                                                          gTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGCAGTCGCGAGGACTCCAGCGCTGGGTGCTCAGCAGGCCATTTTTACCTg                                                                      >  2:804390/1‑238 (MQ=255)                                                                                                                                                                                  ttCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGCCGCGGGGACTCCAGCTCTGGAGGCTCAGCAGGCCATTTTTAACTgcccgccccg                                                             >  2:95234/1‑237 (MQ=255)                                                                                                                                                                                            cccGTTTGAACAAGCTTCCGTCCGTGTGGTGACAGAAAAACCACCTTT‑GC‑CCCTTC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGA‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTa                                                   <  2:953275/239‑1 (MQ=255)                                                                                                                                                                                                         gCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTCCCCGGCCAGTTACCCCGCAACCGGTAAttccc                                      >  2:524364/1‑236 (MQ=255)                                                                                                                                                                                                          ctgccccccccgttggtaCAGAACAATCCCCTTT‑GCTGCTTCC‑TCCCGCCGGGGCTTTGGGTGGC‑GGAATGG‑GCCCCGCGAGGGGTTGCCCCTTCTTTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGGCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTgcg                                      <  1:283832/222‑1 (MQ=255)                                                                                                                                                                                                              cGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGAGGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTCCCCCGTAATCGGTAATTGCGCGGa                                  >  2:279709/1‑238 (MQ=255)                                                                                                                                                                                                                    tgtgGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGGAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGCGGACTCCAGCCCTGGATGCCCGGCGGGCCATTTTTAACTGCCCGGCCAGGTACCCCGTAAACGGGAATTGGCCCGAACAGTTc                           >  1:729000/1‑239 (MQ=255)                                                                                                                                                                                                                          ggagatatagctcccTTT‑GC‑GCCTTC‑CCCGGCCGCTCCTTTGGCGCCA‑TGCAGGGCTCCACAG‑AGGGGTTCACCCATCATTACCTGCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGTTCATCAGGCCATTTTTAACCGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGaaa                     <  1:510835/226‑1 (MQ=255)                                                                                                                                                                                                                          gACAGATAATCCCCCTTT‑GC‑GCCTCC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGaaa                     <  2:493855/239‑1 (MQ=255)                                                                                                                                                                                                                           gacagcaaaaccccgtgggc‑gcTTTC‑ACCCGCCGCGGCTTTGGCTGAA‑TGAGGGGGGTCCCGC‑AGGGGTGGCCCCATCTTTTACCCCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGaaa                     <  1:238018/221‑1 (MQ=255)                                                                                                                                                                                                                           aCAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTCCGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCCGGCCATTTTTAACTCCCCGGCCAGTTCCCCCGTAATCGGCAATGGCGCGGAAAATTTTAGGAAAc                    >  1:196796/1‑239 (MQ=255)                                                                                                                                                                                                                           aCAGATAAATCACCTTT‑GC‑ACCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGCTGCCAGCTCTGGCTGCTCAGCAGGACATTTTTAACTGCCCGCGCAGTTACCCCCTAATCCGTACAGTCGCGGTACATTTCAAGaaaa                    >  1:919284/1‑238 (MQ=255)                                                                                                                                                                                                                            aacgatacactccTTTGGC‑GCCTTC‑TCCCGCCGCGGATTTTGCTGCA‑TGAATGGCTTCCCGC‑AAGGGTTGCCCCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGaaa                     <  2:1069755/227‑1 (MQ=255)                                                                                                                                                                                                                             ataaaaaTCCCTTTT‑GC‑‑CCTTC‑CCCCCGCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAgg                 <  2:667739/235‑1 (MQ=255)                                                                                                                                                                                                                             aaaaaaacccccTTT‑GC‑CCCTCC‑CCCCGCCGGGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCCCCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAg                  <  2:758920/229‑1 (MQ=255)                                                                                                                                                                                                                             aGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGGTGGAGGTCACGGGGACTCCAACCATGGAGGCCCAGAAGGCCCTTTTTAACTGCCCGCACCGTTCCCCCCAAAGCGGGAAATGCGCGGAACATTTCAGGAAACAg                  >  1:387371/1‑239 (MQ=255)                                                                                                                                                                                                                              aaatatccctcTTG‑GC‑CCCTTC‑CCCCGCCGTGGCTTTGGCTGCA‑TGAAGGGCGCCCCGC‑AAGGGTTCCCCCATCATTAACCTCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAgg                 <  2:1132144/229‑1 (MQ=255)                                                                                                                                                                                                                              aaTAAATCCCCTTT‑GC‑GCCTTC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAgg                 <  2:934662/238‑1 (MQ=255)                                                                                                                                                                                                                                tAAATCCCCTTT‑GG‑CCCTTC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCAATAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAgg                 <  2:582062/237‑1 (MQ=255)                                                                                                                                                                                                                                aaatcccccTTA‑GC‑CCCCTC‑CCCCGCCGCGGCTTTTCCTGCA‑TGAAGGGCGTACCCC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAgg                 <  2:34132/230‑1 (MQ=255)                                                                                                                                                                                                                                  aacccccTTT‑GC‑CCCTTC‑CCCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATGAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTaa             <  2:1274734/234‑1 (MQ=255)                                                                                                                                                                                                                                       atttt‑GC‑CCCCTC‑CCCCCCCGCGGCTTTTGC‑GCACTGAAGGGGGCCCCGC‑AAGGTTCGCACCATCATTAACCGCCCCGGTCGGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTAATTATg        <  1:1006986/237‑1 (MQ=255)                                                                                                                                                                                                                                           t‑GC‑GCCTTC‑ACCCGGCGCGGCTTTTGCTGCA‑TGAATGACGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTAAGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGGTCTGGATGCTCAGTAGGCCATTTTTACCTACCCGGCCAGTTACCCCGTACTCGGATCATGCGCGGACAATTTCAGGAAAACGGCTAATAATGcac     >  1:827004/1‑236 (MQ=255)                                                                                                                                                                                                                                           t‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACTGGCTAATAATGCCaa    >  2:422515/1‑239 (MQ=255)                                                                                                                                                                                                                                           t‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAAGTGCGCGGAAAATTTCAGGAAACAGGCAAATTATGCCaa    >  1:154718/1‑239 (MQ=255)                                                                                                                                                                                                                                              c‑gcCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTAATTATGCCAATc  >  2:72540/1‑239 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 GTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGC‑TCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTT‑GATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTT‑GC‑GCCTTC‑ACCCGCCGCGGCTTTTGCTGCA‑TGAATGGCGTCCCGC‑AAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTAATTATGCCAATC  >  NC_000913/2744860‑2745332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑