Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,006,048 G→A 10.2% intergenic (‑264/‑212) ygeV ← / → ygeW putative sigma‑54‑interacting transcriptional activator/putative carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,006,0480GA10.2% 206.4 / 2.0 82intergenic (‑264/‑212)ygeV/ygeWputative sigma‑54‑interacting transcriptional activator/putative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base G (29/42);  new base A (7/2);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 7.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAG  >  NC_000913/3005815‑3006280
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
gATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCaaa                                                                                                                                                                                                                                        <  2:797640/239‑1 (MQ=255)
             gTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGACTTCCCCTTTTTCTTATTTCTGATACTTTACAGCAAATAAATAAAGCGATAAAAAAAACCCAAAGGTTTTTTAttt                                                                                                                                                                                                                          >  2:734884/1‑239 (MQ=255)
                              cATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGGTTTTCAAAATTAAAAGAGATAGACTGCCCCTTTTTTTTATTTTTTTTACTTTGCAGCAAAAAAAAAAAGCGATAAAAAAAACCAAACACTTTTGTATTTTTCAAAAAAACaaaaaa                                                                                                                                                                                                         >  2:595575/1‑237 (MQ=255)
                                   tttttttAACTCAGGTGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCATTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCa                                                                                                                                                                                                    <  1:1187976/234‑1 (MQ=255)
                                        ttAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAAt                                                                                                                                                                                               <  2:1213375/239‑1 (MQ=255)
                                        ttAACGCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAAt                                                                                                                                                                                               <  2:1159514/239‑1 (MQ=255)
                                               aGGGGAAATTGGTTTTGATAAAAACATAAGATTTTTATCAATTTCGAATGAAATTATGGAAGGGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGAt                                                                                                                                                                                        <  1:143877/239‑1 (MQ=255)
                                                 gCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGGGGGTATTAAACTAAATAGAGATAACCCTCCCCCTTATTTTCTTTTTTATATTTTGCGACAAAAAAAAAACACCCTAAAAAAAACCACACCATTTTGTTTTTTACAAAAAACAAAAAACAGGCAACAAatttttgcaa                                                                                                                                                                                      >  1:659130/1‑232 (MQ=255)
                                                 gCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTTAAAAAAAATAACCTGCCCCCTTATCTTATTTCTTATACTTAGCAGCAAAAAAAAAAACCCATAAAAAAATACAAAAAGTTTCGATATTTTCAAAAAAACAACAACTAGCATCAAATtgtgtatt                                                                                                                                                                                      >  2:817156/1‑235 (MQ=255)
                                                                             aTTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAc                                                                                                                                                                                    <  2:81926/213‑1 (MQ=255)
                                                                                 ttATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTGTATCAAAATGAAGAGAGAGAGCCTGCCCCTTTATCTTATTTCTGATAATTAACAGCAAATAAAAAACAAGAGAAAAAAAGCCAAAAGATTTCGTAATTTTCAAACAACCAGAAACTGGACCTAATTTTTGATTAACCCCACACCATCTCCGCCaaaaaagaatttt                                                                                                                                                      >  2:115941/1‑229 (MQ=255)
                                                                                               tGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTTAAGAAAAAAAATATTCCCCTTTTTCTTTTTTTTTATACTTTAAAGCAAATAAAAAACGAGATAAAAAAAAACAAAAGTTTTTTCATTTTTAAAACAAACACAAGCTACAAACCATTTTTTATAAACCCCCCCAAATAAACGTCCAAATAGTCTTTTTCAGACCCCCGGaa                                                                                                                                       >  1:698917/1‑238 (MQ=255)
                                                                                                          aaGAGATATCCCAGTTCTATATCAATATGAGAATTACGGCGGTGAGTTTAGCAAACTGAAGAGAGATAGCCTTCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGGCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGca                                                                                                                             <  2:320353/238‑1 (MQ=255)
                                                                                                          aaGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATTCTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGca                                                                                                                             <  2:511895/238‑1 (MQ=255)
                                                                                                             agaTATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGGTTATTAAAATGAAGAGAGATAACCTGCCCCCTTATTTTATTTTTGATAATTTGCAGCAAATAAATAAAGCGATAAAAAAAAACAAAAGATTTTGTATTTTTCAAACAAACAAAAGCTGGCATCAAATTTTGATCAACACCACACAATTTCCCGCCAATTAATCTTTTTCAGCCGCCGCGGTAATTATTGcacc                                                                                                                           >  2:492888/1‑236 (MQ=255)
                                                                                                              gaTATCACATTTCCATATCAATATGAAAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACt                                                                                                                         <  2:699485/238‑1 (MQ=255)
                                                                                                                 atCACATTTCTATATCAATATGAGAATTACGGCGGTTGGGGTTTTAAACCTGAATAGAAAAGACTGCACCTTTTTTTTTTTTCTGTTTCATATCAGCAAAAAAAAAAAGACAGAAAAAAAACCAAAAGTTTTTGTATTTTTTAAAAAACCAAAAGATGACATAAAATTTTGATTAAACCCCCACCATATACCACAAAATAATCTTTTTCAGACCCACGGAAAAATATTACACACCTAta                                                                                                                     >  1:628/1‑238 (MQ=255)
                                                                                                                   cccaTTTTTTTTTCAATTTGTGAATTTGGGGGGTGATTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGAAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt                                                                                                                   <  1:595575/237‑1 (MQ=255)
                                                                                                                   cacaGTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATGACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAccc                                                                                                                                                                                  <  2:128228/177‑1 (MQ=255)
                                                                                                                   aacaagcttattTCAATATGAGAATTTCGGGTGTTAGTTTAACAAACTGAAAAAGGATAGCCTGCCCCTTTAACTTATTTCTGATACTTAGCAGCAAATAAATAACGCTATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt                                                                                                                   <  1:1225798/231‑1 (MQ=255)
                                                                                                                        ttttttatCAATTTTAGAATTTCAGCGTTGATTTTATAAAACTGAAAAAAAATAGACTTCCCCTTTAACTTATTTCTTATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc                                                                                                              <  1:1004540/234‑1 (MQ=255)
                                                                                                                        ttCTTTACCAATTTGAGAATTACGGGTGTGTATTTATCAAACTGAAGAGAGAAGGCCTGCCCATTTAGCTGATTTTTGATACTTCGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCGTGCATCAATTTGTGATCAACCCAACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTGATTGTTAGTc                                                                                                              <  1:817156/239‑1 (MQ=255)
                                                                                                                        ttCTATATCAATATGAGAATTACGGCGGTGAGTTTATTAAACTTAAGAGAGATAACCTGCCCCCTTATTTTATTTCTGATAATTAACAGCAAATAAATAACACGCTAAAAAAAACCCAACCGTTTTGTATTTTTCAAACAACCAGAAACTGGACTCCACTTTGGATCAAACCCACACATTATCCGTCCAATTAACCTTTTTCTGCCGCCCCGGTAATTCTTGTACCCCTATTTTTTGt                                                                                                               >  2:139631/1‑238 (MQ=255)
                                                                                                                          cTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTccc                                                                                                            <  2:896983/239‑1 (MQ=255)
                                                                                                                                           ttACGGGGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGaaaa                                                                                           <  2:483372/239‑1 (MQ=255)
                                                                                                                                                            aTCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGc                                                                           <  2:659130/239‑1 (MQ=255)
                                                                                                                                                                atcttaagagaattaagcTGCCCCTTTATCTTAATTCTGATACTTAGCAGGAAATAAATACCGCGATAAAAAAAGCCAAAGTGTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTGTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGt                                                                       <  1:3374/222‑1 (MQ=255)
                                                                                                                                                                aaCTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGt                                                                       <  2:628/239‑1 (MQ=255)
                                                                                                                                                                  atGAAAAGAAATAACCTTCCCCTTTATTTTATTTTTGATTCTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGTGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTca                                                                    <  1:492888/238‑1 (MQ=255)
                                                                                                                                                                    gAAGAGAAATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTcaca                                                                  <  2:480417/239‑1 (MQ=255)
                                                                                                                                                                     aaGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAg                                                                 >  2:3374/1‑239 (MQ=255)
                                                                                                                                                                     aaGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAg                                                                 >  2:301441/1‑239 (MQ=255)
                                                                                                                                                                      agagagATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAg                                                                 <  2:1174935/238‑1 (MQ=255)
                                                                                                                                                                            aTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATa                                                          >  2:204386/1‑239 (MQ=255)
                                                                                                                                                                               gCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGACAACACCAATCACTTTGGCAAGTCACAGTGAAATaaa                                                        >  2:729763/1‑238 (MQ=255)
                                                                                                                                                                               gCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAAc                                                       >  2:250124/1‑239 (MQ=255)
                                                                                                                                                                                 cttccccTTATTTTTTTTTTTGATACTTACACGCAAAAAAATAACGCGATAAAAAAAGCACAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCTCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCa                                                     <  1:120065/236‑1 (MQ=255)
                                                                                                                                                                                    ccccTTTATCTTCTTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGTTCACCCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATCATTCTGGCACACTTATGCTTCTTCCCAGGTATAGCTGTGAAAACCCAAATCACTTTGGCAAGCCACAGGGAAAAAAAACACtt                                                  >  1:24648/1‑239 (MQ=255)
                                                                                                                                                                                    ccccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgc                                                                                                                                             >  2:497616/1‑149 (MQ=255)
                                                                                                                                                                                    ccccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgc                                                                                                                                             <  1:497616/149‑1 (MQ=255)
                                                                                                                                                                                    ccccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACtt                                                  >  2:789870/1‑239 (MQ=255)
                                                                                                                                                                                    ccccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGGGAAATAAACCACtt                                                  >  2:1276608/1‑239 (MQ=255)
                                                                                                                                                                                    ccccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAAAACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACtt                                                  >  2:385801/1‑239 (MQ=255)
                                                                                                                                                                                    ccccTTTATCTTATTTCTGATACTTAGAAGCAAATAAATAGCGCGATATAAAAAGCCAAACGTTTTCGTCTTTCACAAACAACCAGACGCTGGCATCAATTTGTGATCAACCCAACACATTATCCGTCAACTTAGACTTTTGCAGCCGCGCGGATAATCCTGGCACACTTCTTGTTAGTCCCAGGTATAGCTGGGAAAAAACCAATCACTTTGTAAAGACAAAGTGAaaaaaaaaaata                                                  >  1:1058872/1‑233 (MQ=255)
                                                                                                                                                                                     cccTTTATCTTATTTCTGATACTTAGAAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACttt                                                 <  2:657214/239‑1 (MQ=255)
                                                                                                                                                                                       cTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAAGTTGTGATCAACCCCACTCATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAg                                                                 <  2:1155703/221‑1 (MQ=255)
                                                                                                                                                                                        tttATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGcc                                              <  2:856942/239‑1 (MQ=255)
                                                                                                                                                                                          tATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAACCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGTGACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAAAACCAATCACTTTTGAAAGTCACAGTGAAAAAAACCACATTGCCTg                                            >  1:861991/1‑239 (MQ=255)
                                                                                                                                                                                           tttttATTTTTTATACTTAAAAGGAAATAAATAGCGCGATAAAAAAAGCCAAACGTTTTCGTATTTTAAAAAAAAACAGAAAGTGGCATCAACTTGTGATCCACCCCACCCATTATCCGTCAAATTAGGCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGt                                           <  1:1064616/236‑1 (MQ=255)
                                                                                                                                                                                           tttctATTTTTGATTCTTAGCAAGAAAATAAAAACCGGATAAAAAAAGCCAAACGGTTTCCTTTTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGTCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGt                                           <  1:1214993/235‑1 (MQ=255)
                                                                                                                                                                                           aTCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGt                                           <  2:673260/239‑1 (MQ=255)
                                                                                                                                                                                                 tttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGCAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTATTCCCAGGTTCAGCTGTGACAACCCAAACCACTTTGGAAAGTACCAGTGCACAAAAccctgtttcttaaacacaa                                     >  1:1155703/1‑222 (MQ=255)
                                                                                                                                                                                                 tttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTcc                                     >  2:1019549/1‑239 (MQ=255)
                                                                                                                                                                                                 tttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTc                                      >  2:764973/1‑238 (MQ=255)
                                                                                                                                                                                                 tttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTc                                      >  2:71270/1‑238 (MQ=255)
                                                                                                                                                                                                 tttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACAAGAAGCTGGCATCAATTTGTGATCAACACCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATACTTCTGGCACACTTATTGTTATTCCCAGGCATACTTTTGAAAAAACCAATAACTTTGGCAAGTACACATGAAAAAAAACCATTTGCCttcaattcc                                     >  1:314003/1‑231 (MQ=255)
                                                                                                                                                                                                  ttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCa                                    <  2:861991/239‑1 (MQ=255)
                                                                                                                                                                                                      tatatttgacagtaAATAAGTAACGCGATAAAAAAACCCAAAGCTCTTCGTATTTAACCACAAACGCGAAGGTGACATCAATTTGGGATCAACCCGACTCATTATCCGTCAAAGTAGGCGTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCCCTTTGGCAAGTCACAGTGAAATAAACCACTTTGCATGTCATTCCACttc                                <  1:17067/230‑3 (MQ=255)
                                                                                                                                                                                                      gATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGCCAAGTCACATTGAAATAAACCACTTTGCCTGACATTCCACTa                                 >  2:567802/1‑238 (MQ=255)
                                                                                                                                                                                                             agcagcAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGCCt                         >  2:987750/1‑239 (MQ=255)
                                                                                                                                                                                                             agcagcAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGACGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCCCAGTGAAATAAACCACTTTGACTGTCATTCCACAACCGGGACt                         >  1:1284585/1‑239 (MQ=255)
                                                                                                                                                                                                                catcaAAAAAAAAAAGCGATAAAAAAAGACAAACGTTTTCGTATTTTCCAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAGTTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGGCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACttt                       <  1:1019549/236‑1 (MQ=255)
                                                                                                                                                                                                                   caAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTg                                                               <  2:1288129/196‑1 (MQ=255)
                                                                                                                                                                                                                     aataaataACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCAGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGAAACCTTATTGTTAGTCCAAGTGTTAGCTGTGAAAAAACAAATCACTTTGGCAAGTACCAAGGAATTAAAACACTTTGCCAGTAATTCCACACCCGGGACTTTatgat                  >  1:1001705/1‑239 (MQ=255)
                                                                                                                                                                                                                        aaataaCGCAATAAAAAAAGCCAAACGTTTTCCTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGaa               <  2:158607/239‑1 (MQ=255)
                                                                                                                                                                                                                             aCGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATaa                                                                                                                                     >  2:13904/1‑117 (MQ=255)
                                                                                                                                                                                                                             aCGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATaa                                                                                                                                     <  1:13904/117‑1 (MQ=255)
                                                                                                                                                                                                                             aCGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGaaa              >  2:266681/1‑235 (MQ=255)
                                                                                                                                                                                                                              cgcgATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGt         >  2:750636/1‑239 (MQ=255)
                                                                                                                                                                                                                              cgcgATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAAAACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATTATGaaaaaagt         >  1:718821/1‑235 (MQ=255)
                                                                                                                                                                                                                                cgttAAAAAAAACCAACTTGTTTCTAATTTTACAATCAGCCAGAAGCTGGCATGAATGTGTGATCAACACCACTCATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGAGACTTTATGATGAAAACTGTTa       <  1:385801/236‑1 (MQ=255)
                                                                                                                                                                                                                                cgATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCAACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACAAATCACTTTGGCAAGTCACAGTGAAATAAACAACTTTGCCCGTCTTTCCAACACCGGGCATTTCAGATAaaaaacttta       >  1:837885/1‑233 (MQ=255)
                                                                                                                                                                                                                                  aTAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGTCAATTCTGGCACACTTATTGTTAGTCCCAGGTATACCTTTGAAAACACCACTACCTTTGGCCAGTCCCAGGGAAATAAACCACTTTACCTGTCATTCCCCTCCCCGGCCTTTAATAAAAAAACTGTAAAt     >  1:283546/1‑239 (MQ=255)
                                                                                                                                                                                                                                  aTAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAAt     >  2:795246/1‑239 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaGCCATATGTTTTCGTATTTTACAAACAAACAGAAGCTGGCATCAATTTGTGATCAACCCCACTCATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTAGTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGGCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAg  <  1:764973/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaGCCAAACGTTTTCGTTTTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAg  <  2:1114494/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAg  <  2:1058872/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAg  <  2:837885/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAg  <  2:718821/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAg  <  2:698917/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAg  <  2:328755/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaACCAAACTTTTTCGTTTTTTACAAACAACCAGACGTTGGCAACAATTTGGGCACAAACCAACACTGTATCCGTCAAAGTAGTCTGTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGCACTTTATGATGAAAACTGTTAATGAg  <  1:734884/239‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
GATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAG  >  NC_000913/3005815‑3006280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: