Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,266,745 T→C 10.2% I107V (ATT→GTT)  tdcA ← tdc operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,266,7450TC10.2% 243.6 / 2.4 102I107V (ATT→GTT) tdcAtdc operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (43/43);  new base C (2/9);  total (45/57)
Fisher's exact test for biased strand distribution p-value = 5.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGC  >  NC_000913/3266513‑3266982
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    
gTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAAACTGCGCTTTCGGGAAAACCACTTTGACCTTG‑TTGAT‑CCTCCCTGACA‑TAAAAACAAAACacatcaataa                                                                                                                                                                                                                                                >  1:973876/1‑237 (MQ=255)
   cATTTTCGGGACTTCATGGCTACCAGCCCAAACTCGGACCCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGAGTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAgg                                                                                                                                                                                                                                             <  1:974051/239‑1 (MQ=255)
    aTGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAGCCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAgg                                                                                                                                                                                                                                             <  2:1249478/238‑1 (MQ=255)
    aTGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAgg                                                                                                                                                                                                                                             <  2:4299/238‑1 (MQ=255)
       ttcgtgtatttctggtcaacAGCACCAACTTCGACCCGAACAGCGGCTCAACCTTAAAATCCTGAAGCTTCATTTGTTCCCTTAACGTAACAATCGCAAAA‑T‑CCAGCCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGaaaa                                                                                                                                                                                                                                         <  1:1106677/220‑1 (MQ=255)
           ggACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAAAACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAACCACTGAAGGAAAACCaa                                                                                                                                                                                                                                     >  2:184990/1‑239 (MQ=255)
              cttaacggttACCAGCACAAACTCGGACTCGAACAGGTGCTCAACATGTAAAGCCGTAAGCTTCATTTGTGCACCTAACGGACCAATCGCAAAA‑T‑CCAGGCGACCGTCGCGGATTG‑ACGGTAAGAACGAAGACAGTTGCTCGTCATACATAGAAACCTGCGCTTTCGGGAACACCTCATTGAACGTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑At                                                                                                                                                                                                                                  <  1:742796/229‑1 (MQ=255)
               ttACTTGCCACCAGCACAAACTCGGACCCGAACAGCGGCCTAACATGTAAATCCGGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCATCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑g                                                                                                                                                                                                                                <  2:778228/238‑1 (MQ=255)
                ttccggCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGGTTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑gag                                                                                                                                                                                                                              <  2:858948/235‑1 (MQ=255)
                tACTGGCTACCACAACAAACTCGGACACGAACAGCGGCTCAAACGGTAAATCCTGAAGCTTAATTTCTGCCCTTAACGTACCAAGCGAAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑gag                                                                                                                                                                                                                              <  1:839833/239‑1 (MQ=255)
                 aCTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGACATTG‑TTGAT‑CATCCCTGCCA‑TAAAAGTAAACCCATTCAATAAAGGAAAACCAA‑AT‑gag                                                                                                                                                                                                                              >  2:134066/1‑238 (MQ=255)
                 aCTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑gag                                                                                                                                                                                                                              >  2:734512/1‑238 (MQ=255)
                 aCTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑gag                                                                                                                                                                                                                              >  2:839833/1‑238 (MQ=255)
                         ccAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTCCGCTTTCGGGCACACCTCTTTGCACTTG‑TTCAT‑CATCCATTACC‑TAAAAGGAAAACCCATCAATGAAGAAAAAACAA‑AA‑GACAA‑ATccacc                                                                                                                                                                                                                    >  1:680543/1‑239 (MQ=255)
                           acacaaaacgtggacaggAACAGTGGCTAAAAATGTAAATCCTGAAGCTTATTTTCTGACCGTAACGAACCACACGCAAAA‑T‑CAATGCGACCGCGGCGGATTG‑CGGGTAAGACCGAAGACAGTTGCGATTCATCCATAGAACCCTGCGTTTTCGGAAACACCTCTTGAAACTTG‑TGTAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAAGGAAGGAAAACCAA‑AT‑GAGAC‑TTCcacc‑ac                                                                                                                                                                                                                 <  1:734512/222‑1 (MQ=255)
                           aGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTccacc‑a                                                                                                                                                                                                                  >  2:1096795/1‑238 (MQ=255)
                             cacaAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCCCTTCATACATAGAAACCTGCGCTTTCGGGAACACCCCTTTGAACTTG‑TTGAT‑CATCCCTGCAC‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAA‑TTCAaca‑acc                                                                                                                                                                                                                >  1:1288245/1‑238 (MQ=255)
                               caAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTT‑CCGGTACGAACGAAGACAGTTGCTCTTCATACATAGAAACCTGCGCTTTCGGGAAAACCTCTTTCAACTTG‑TTAAT‑AGACCCTGCAA‑TAAAAGTAAAACCAATCAATGAAGATAAACCAA‑AA‑CAGAA‑TTacacacccac                                                                                                                                                                                                               >  1:717371/1‑232 (MQ=255)
                                 aaCTCGGACCCGAACCGGGGCTAAACATGTAAATCCTGAAGCTTCATTTCGGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCCACCGGCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACCCCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTc                                                                                                                                                                                                           <  2:1213743/239‑1 (MQ=255)
                                   cctggACCCGAACAGCGGCCAAACTTGAAAATCCTAAACTTTATTTTTTGCCTTTAACGTACCAATCGAAAAT‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAAAACGAAGACATTTGCGTTTCATACATAGAACCCTGCGCTTCCGGGAACACCTCTTTGAACTGG‑TTGAT‑AATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AG‑GAGAC‑TTCCACC‑ACCGCCTCag                                                                                                                                                                                                         <  1:787552/236‑1 (MQ=255)
                                         cTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTGCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAACCAATGAAGGAAACCCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACa                                                                                                                                                                                                   <  2:182225/239‑1 (MQ=255)
                                                 cggggtaaaCGTGTAAACCCTGAATCTTAATTTCTGCATTTAACGGACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCCTTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCtt                                                                                                                                                                                           <  2:267125/232‑1 (MQ=255)
                                                  cGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCAAC‑ACCGcaccaaaaaaaaaaaccgtta                                                                                                                                                                                          >  1:490906/1‑219 (MQ=255)
                                                   ggcccaaatgtaaaaGCCTAAAGTTTACTTTCCCCACTTAACCAACCAACCGAAAAA‑T‑CCGTTGCACGGTCCGGGATGC‑CCGGTAAAAAGAAAGACGCTTGCTCTCAATCCAAAGAACCCTCCGTTTCCGGGACCACCCCTTTGAACTTG‑TTGAC‑CATCCCTGCCA‑TAAAAGTAAAACCAATCAATAAAGGAAAACCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTAt                                                                                                                                                                                         <  1:1291679/227‑1 (MQ=255)
                                                        aacagtaaaaTCCGGAAGCTTCATTTCTGCACTTAACGTACCAATCGAAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAACCCGGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAACCCAATCAATGAAGGAAACCCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCAt                                                                                                                                                                                    <  2:459107/232‑1 (MQ=255)
                                                                    cTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAACAATACCCCTTATCCAATAAACCAAAtttt                                                                                                                                                                        >  1:101380/1‑239 (MQ=255)
                                                                     ggagactttgttttTGCCCTTAACGTACCAATCGCAAAA‑C‑CCAGTCGCCCGTAGGGGATTG‑CCGGTAAGAACGAAGACAGTGGCTCTTCATACATAGAAACCTGCGCTTTCGGAAACACCTCTTTGAACTGG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAACCAATGAGGAAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATAttttt                                                                                                                                                                       <  2:968115/229‑1 (MQ=255)
                                                                       aaGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTTAT‑CATCCCTGACA‑TAAAAGTAAAACCAACCAATGAAGGAAACCCAA‑AT‑GGGAC‑‑TCCACCAAACGCCTCACAAAACAAACGGACTATCTAATTAACCATCtttttta                                                                                                                                                                     >  1:762051/1‑237 (MQ=255)
                                                                       aaGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTtttca                                                                                                                                                                     >  2:1150869/1‑239 (MQ=255)
                                                                          cttcttTTCTGCCATTACCGAACAAATCCAAAAA‑T‑CCATGCGCCCTGCGCGGATTG‑CCGGTAAGAAAGAAGACAGTTGCTCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAAGTTG‑TTGAT‑CATCCCTAACA‑TAAAAGAAAACCCAACCAATGAAGAAAAACCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTtttcattt                                                                                                                                                                  <  1:731473/239‑1 (MQ=255)
                                                                           ttCATTTTTGCACTAAACGATCCAAGC‑CAAAA‑TCCCAGTCGACCCTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGTTTCATACATAAAAACCTGCGCTTCCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGAAA‑TAAAAGTAAAACCAATCAATGAAGGAAACCCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTtttcatttc                                                                                                                                                                 <  2:1007850/239‑1 (MQ=255)
                                                                            ttatttctaactttAACGTACCAAACGAAAAA‑C‑CCCTGGGACGGTGGCGTATGG‑CCGGAAAGAAAGAAAACAGTTGCGTTTCATAAAAAAAACCCTGGGTTGTGGGGAACCCCTC‑TTGAACCTGTTTGAT‑CATCCCTCACA‑AAAAAGTAAAACCAATCAATGAAGAAAAACCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTtttcatttca                                                                                                                                                                <  1:142643/227‑1 (MQ=255)
                                                                                     cACTTAACGTACCAATTGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATg                                                                                                                                                      >  2:477835/1‑239 (MQ=255)
                                                                                     cACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGGCAGTTGCGCTTCATACATATAAACCTGCGCTTTCGGGAACACCACATTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAACGTAAAACCATTCACTGAAGGAAAACCAA‑AT‑CAGCC‑CTCAACC‑ACCGCCACAGCAGACAAACCGCTTACCTTATAAACCACATTTTACTTTTCA‑Agggaatta                                                                                                                                                      >  1:699392/1‑234 (MQ=255)
                                                                                       cTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACAAACCGCTTATCTCTTTACCCATA‑TTTTCATTTTACCGGGAAATGGa                                                                                                                                                    >  2:933030/1‑239 (MQ=255)
                                                                                         taccGTCCCAATCGCAAAA‑C‑CCATCCGACCGTCGCGGATTG‑CCGGAAAAAACGAAGCCAGTTGCCCTTCAAACATAGAACCCGTCGCTTTCGGAAACCCCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAACCCAAACAAGGAAGGAAAACCAA‑AG‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGAtt                                                                                                                                                  <  2:587103/236‑1 (MQ=255)
                                                                                         tAACGTACCAATCGAAAAA‑C‑CCAGTCGACCGTCGGGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAACCCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGAtt                                                                                                                                                  <  2:973876/239‑1 (MQ=255)
                                                                                          aaCGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGGAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGCAGACATACCGCTTAACTCATTAACCAAAATTTTAATTTAA‑CGGGaaaatgaagc                                                                                                                                                 >  1:591154/1‑232 (MQ=255)
                                                                                               ccaaagcGCAAAA‑T‑CCAGTCCGCCGGCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATGAATAGAACCCGGCGCTTTCGGGAACCCCTTTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAACCCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGaa                                                                                                                                             <  2:769368/232‑1 (MQ=255)
                                                                                               aCCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCAACC‑ACCGCCTCAGAAGACATACCGCTTCTCTCATTACCCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAAc                                                                                                                                            >  2:683322/1‑239 (MQ=255)
                                                                                               aCCAACCGAAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACAAAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGaa                                                                                                                                             <  2:101380/238‑1 (MQ=255)
                                                                                                 atagcagaaaa‑C‑CCAGTCCACCGTCGGGGTTGG‑CCGGTAAGAAGGAAGGAAGTTGGGTTTAATACATAGAACCCTGCGCTTTCGGGAACCCCTCTTTGAACTTG‑TTAAT‑CAACCCTGATA‑AAAAAGTAAAACCAACAAATGAAGGAAAACCAA‑TT‑GAATC‑TCACACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATGCGGAACgg                                                                                                                                          <  2:947116/232‑1 (MQ=255)
                                                                                                    tCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCAt                                                                                                                                                                                    >  1:722417/1‑195 (MQ=255)
                                                                                                    tCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCAt                                                                                                                                                                                    <  2:722417/195‑1 (MQ=255)
                                                                                                    acGCAAAACT‑CCAGCTGATCGGTGCGGATGT‑GCGGTAAGAACGAAGACAGTTGCGTTTAATACATGAAAACCTGCGTTTGCGGGAACACCTCTGTGAACTTG‑TTAAT‑AATCCCTGAAA‑TAAAAGTAAAACCAATAAATGAAGTAAAACCAA‑TT‑GAGAC‑TTCCACC‑AACGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGga                                                                                                                                        <  2:524303/238‑1 (MQ=255)
                                                                                                         aaa‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTAATCCAATTACCCCTATTTTTCATTTAA‑CAGGTAATGGATACGGAACGGGAGAGaaa                                                                                                                                  >  1:423098/1‑239 (MQ=255)
                                                                                                                    tggccgctggcgATTGCCCGGTAAAAATAAAGACAGTTGCACTTAATACAAAGAAACCTGCGCTTTCGGGAACACCGCTTTGAATGTG‑TTGAT‑CAACCCTGAAA‑TAAAAGAAAACCCAATAAATAAAGAAAACCAAA‑AT‑ACGAC‑TTCCCCA‑ACCGCCTCAGAAGACATATCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGAc                                                                                                                          <  1:267578/228‑1 (MQ=255)
                                                                                                                       ccgtctggatttG‑CCCGTAACGACGAAAACAGTCGCCCGTCAGACATAGACACCTGTGCTTCCGGTAACACCGCTTGGACATTC‑ATGAT‑CATCCCGGACA‑AAAAAATAAACCCAAGCATTGAAGAAAAACCAA‑TT‑AAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATGAACCATATTTCTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGc                                                                                                                      <  2:1248391/229‑1 (MQ=255)
                                                                                                                       ccGTCGCGGTTTG‑CCGGTAAGAACGAAGACATTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGc                                                                                                                      <  2:1052160/239‑1 (MQ=255)
                                                                                                                       ccGGCGGGGTTTG‑CCGGAAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCCTTTTCGGGAACCCCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGc                                                                                                                      <  2:490906/239‑1 (MQ=255)
                                                                                                                        cGTCGGGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTtttcatttca                                                                                                                                                                <  2:8846/197‑1 (MQ=255)
                                                                                                                        cGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTtttcatttca                                                                                                                                                                >  1:8846/1‑197 (MQ=255)
                                                                                                                        cGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCC‑CGGGAAAAGGATTCGGAACGGGAGGGTAACAATTTACCGGCa                                                                                                                     >  1:1241565/1‑239 (MQ=255)
                                                                                                                        cGTCGCAGATTG‑CCGTGAAGAACGAAGACAGTTCGGTTTCATACATAAAACCCTGCGCTTCCGGGAACACCTTGTTGAACTTG‑TTGAT‑CATCCCTGCCA‑AAAAAGTAAACCAAATCAATGAAGGAAAACCAA‑AT‑GAGTC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGc                                                                                                                      <  2:179262/238‑1 (MQ=255)
                                                                                                                         gTCGCGGATTG‑CCGGTAAGAACGAAGCCAGTTGCGCTTCATAAAAAGAAACCTGCCCTTTCGGGAACCCCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAg                                                                                                                    <  2:1288245/239‑1 (MQ=255)
                                                                                                                          aatgtgtataagaaacaggAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACCCCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCa                                                                                                                     <  2:284358/220‑1 (MQ=255)
                                                                                                                             gggATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATAAATAGAAACCTGCGCTTTCGGGAACCCCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑AAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGgtgt                                                                                                                <  2:591154/238‑1 (MQ=255)
                                                                                                                              ggATTG‑CCGGTAAGAACGAAGACATTTGCGCTTCAAACAAAAAAACCGGCGCTTTCGGGAACACCTCTTTGAATTTT‑TTGAT‑CATCCCTGACA‑AAAAAGTAAAACCAATCAATGAAGAAAAACCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGgtgt                                                                                                                <  2:1000887/238‑1 (MQ=255)
                                                                                                                              ggATTG‑CCGGAAAGAACGAAGACAGTTGCGCTTCATACATAGAACCCTGCGCTTTCGGGAACCCCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGgtgt                                                                                                                <  2:708728/238‑1 (MQ=255)
                                                                                                                                                   gACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATTTTACAACGGGAtttt                                                                                            >  1:929196/1‑238 (MQ=255)
                                                                                                                                                       tttgccgttaatcaaaaaaaaCCTGGCGTTTCGGAAACCCCTCTTGGAACTTT‑TTGAT‑CACCCCTGAAA‑AAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGc                                                                                       <  2:593489/221‑1 (MQ=255)
                                                                                                                                                       gTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCC‑CGGGGAATGGATTCGGACCGGGAGAGTAACAATTAACCGGCAGGGGTTAATGtctgtctttttaaaaaatcc                                                                                       >  1:284358/1‑220 (MQ=255)
                                                                                                                                                         tGCGCTTCATACAAAGAAACCTGGCCTTTCGGGAAACCCTCTTTGAACTTT‑TTGAT‑CATCCCTGACA‑AAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGc                                                                                       <  2:680543/237‑1 (MQ=255)
                                                                                                                                                             ttttaaaccaaGAAACCCGCTGTTTCGGGAAC‑CCCCTTTGAAATTT‑TTTATAAACCCCTTACA‑AAAAAATAAAACAAAACAATGAAAGAAAACCAA‑AA‑G‑GACTTTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACt                                                                                 <  1:933030/229‑1 (MQ=255)
                                                                                                                                                             cTTCACACATAAGATCCGGCTATTGCGGGAACACCT‑TTTGAAAGTGTTTGAT‑CAGCCTGTAAA‑TAAAAGTAAACACAACAAATG‑AGGAAAACATA‑ATGGGGAC‑TCCCGCC‑GCCGCCTCAGATGACATACCGCTTATCGTATTAACCATATGTGACATTACA‑GGGGTAATGGATTCGGAACGGGAGAGTAACAATTTACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACt                                                                                 <  1:1052802/239‑1 (MQ=255)
                                                                                                                                                                catacataGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAt                                                                              >  2:127877/1‑239 (MQ=255)
                                                                                                                                                                catacataGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAt                                                                              >  2:649723/1‑239 (MQ=255)
                                                                                                                                                                catacataGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGGGTTAATGTTACAACGGGATTTTTCCGCACCACTaaa                                                                              >  1:61506/1‑238 (MQ=255)
                                                                                                                                                                 atacataGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGAAATGGATTCGGAACGGGAGCGTACCAATTGACCGGCAGGAGTTAATGTTACACCGGTATTTTTCCGCACCACTAAt                                                                              >  2:1052802/1‑238 (MQ=255)
                                                                                                                                                                      taGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGGGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCcac                                                                        >  2:1187501/1‑238 (MQ=255)
                                                                                                                                                                          aaCCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAATAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAAACATACAGCTTATCTCATTAACCATATTTTTCATTTAC‑CCGGTAATGGATTCGGACAGGGAGAGAAACAATTGACCGGAAGAGGTAAAAGTAACACCGGAATTTTTCCGCCCCACTaaaccaacaacaa                                                                    >  1:113756/1‑228 (MQ=255)
                                                                                                                                                                           aCCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGTAGAGTAACAATTGACCGGCAGGGGTTAATGTTAACCCGGGATTTTTCCGcaaaccaaattcacccccaaa                                                                   >  1:166231/1‑220 (MQ=255)
                                                                                                                                                                            ccggcgtTTTCGGGAACCCCTTTTTGAACTTG‑TTGAT‑CAACCCGGCAA‑TAAAAGTAAAACCAATCAATGAAGGAAAACAAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCaaaa                                                                 <  2:112034/236‑1 (MQ=255)
                                                                                                                                                                              tcgcttttCGGGAACCCCTTTTTAAATTTT‑TTTAT‑CACCCCT‑ACACAAAAAGAAAAACCAACCAATTAAGGAAACCCAA‑AT‑GCACC‑TACCCCC‑ACCGCTTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAaata                                                               <  1:649723/234‑1 (MQ=255)
                                                                                                                                                                              tGCGCTTTCGGAAACCCCTTTTTGAACTTT‑TTGAC‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAaata                                                               <  2:166231/239‑1 (MQ=255)
                                                                                                                                                                                cgcTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAaataat                                                             >  2:946673/1‑239 (MQ=255)
                                                                                                                                                                                cgcTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCACACCACTAAT‑TCCACACCAAaataaa                                                             >  2:167190/1‑238 (MQ=255)
                                                                                                                                                                                cgcTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGGATTTTTCCGCCCCACTAAT‑TCCACACCAAaataat                                                             >  2:387411/1‑239 (MQ=255)
                                                                                                                                                                                         gAACCCCTCTTTGAACTTG‑TTGAT‑CACCCCTGACA‑TAAAAGTAAAACCAATCAAAGAAGGAAAACCAA‑AT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ata                                                    <  2:929196/238‑1 (MQ=255)
                                                                                                                                                                                               ccctttttgactgtttttgccctcCCTGAAA‑AAAAGGTAAACACAACAAATGTAGGAACACGAA‑AA‑GAGAC‑TTCCACC‑TCCGCCTCAGAAGACATTCCGCTTATCTCATTAGACAATTTTGTCCTTTCA‑CGGGTAAGGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACGCCGGTATTTTTCCCCGCCACGAAT‑TCCACACCAAAATAACCTTCA‑ATATCGtt                                               <  1:847987/217‑1 (MQ=255)
                                                                                                                                                                                               cattttGAACTTG‑TTCAC‑CCTCCCTGACA‑AAAAAGAAAAACCAATAAATAAGAGAAAACCAA‑CT‑GAGAC‑TTCCCCC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTaa                                             <  1:1087929/236‑1 (MQ=255)
                                                                                                                                                                                                 cctttttacttttttattatccccTGACA‑AAAAAAAAAAACCAACAAATGAAGAAAAACCAA‑AG‑GTGAC‑TCCCCCC‑CCCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTaa                                             <  1:1228585/218‑1 (MQ=255)
                                                                                                                                                                                                      ttaattgtttaactccccccGACA‑AAAAATAAAAACAAACCAATGAGACAAAACCAA‑AG‑GATCC‑TTCCCCC‑ACCGCCTCAGAATACATACCGCTTATCCCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATACGGAACGGGAGAGTAACAATTGGCCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGAtt                                        <  1:946673/223‑1 (MQ=255)
                                                                                                                                                                                                         ttg‑ttgAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCCTTAACCATATTTTTCCTTTCA‑CGGGAAATGGATTCGGACCGGGAGATCAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTATCTCACTACAAAT‑TACACACCCAAACAATATTTA‑AATTCGTTAAAGATTTTAc                                    >  1:368771/1‑238 (MQ=255)
                                                                                                                                                                                                           g‑ttgAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTg                                  >  2:665199/1‑238 (MQ=255)
                                                                                                                                                                                                            ttttattcttcccctcta‑TAAAAAAAAAACAAATAAAAAAAGAAACACCAATGT‑GAACC‑TACCACA‑A‑CGCCCCAGAAGACATTCCGCTTAACTCATTAACCATATTTTGCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTg                                  <  1:1096795/222‑1 (MQ=255)
                                                                                                                                                                                                             ttgAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTgct                                >  2:1087929/1‑237 (MQ=255)
                                                                                                                                                                                                             ttgAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGGATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACt                                   >  2:1228585/1‑236 (MQ=255)
                                                                                                                                                                                                                 atccccccTTACA‑AAAAAATAAAACAAAAAAAGAAAGGAAAACCAA‑AT‑TAGTC‑CTCCACC‑CACGCCTCAGAAGACATTCCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTGACGGc                             <  1:1187501/234‑1 (MQ=255)
                                                                                                                                                                                                                   catcCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTGACGGCCg                           <  2:440937/239‑1 (MQ=255)
                                                                                                                                                                                                                    atcCCTGACA‑TAAAAGTAAAACCAAACAATGAAGGAAACCCAA‑AT‑GAGAC‑TCCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTGACGGCCgg                          <  2:423098/239‑1 (MQ=255)
                                                                                                                                                                                                                     tcCCTAACA‑AAAAAGTAAACCCAATCAATGAAGGAAAACCAA‑AG‑GACAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTGACGGCCGGt                         <  2:967529/239‑1 (MQ=255)
                                                                                                                                                                                                                      cccTGACA‑TAAAAGTAAAACCAATCAATGCAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGACTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGGGTTAAAGTTAACCCGGTCTTTTTCGGCA‑CACTTATATCACCACCAAAAAAATATTCA‑AAATCATTaagtctgtccttaatacacaact                        >  1:30746/1‑218 (MQ=255)
                                                                                                                                                                                                                       ccTGACA‑TAAAATTAAACCCATTCAAGAAAGGAAAACCAA‑AT‑‑AGACTTTCCACC‑GCCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTGACGGCCGGt                         <  2:1172467/237‑1 (MQ=255)
                                                                                                                                                                                                                           aCA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGGATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTGACGGCCGGTTGAGtt                   >  2:1300345/1‑239 (MQ=255)
                                                                                                                                                                                                                                aaaaGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTAAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGCGAGTAACAATTGCCCGGCAGGCGTTAATGTTTCACCGGTATTTTTCCGCAACACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCTTTAATTCTTTTAcatcagccaggtttagtaaaa                >  1:965181/1‑218 (MQ=255)
                                                                                                                                                                                                                                        aaCCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTcc                                                        >  1:698659/1‑190 (MQ=255)
                                                                                                                                                                                                                                        aaCCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCa                                                        <  2:698659/191‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGGGTTAATGTTACACCGGTATTTTTACGCCACACTAAT‑TTCACACCAAAATAAACTTCA‑ATATCGTTAATGATTTTCTTGACACCCAGATAAGTTAACCCAAATACt       >  1:206198/1‑239 (MQ=255)
                                                                                                                                                                                                                                             atcaatGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGAGTCGGAAAGGGAGAGAAACAATTGACCGGCAGGGGTTAAAGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCACATA‑CGTTAATGATTTTTATTACGGCCGGTTGAGGTAAACCACATTAttttta  >  1:976687/1‑237 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    
GTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAA‑T‑CCAGTCGACCGTCGCGGATTG‑CCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTG‑TTGAT‑CATCCCTGACA‑TAAAAGTAAAACCAATCAATGAAGGAAAACCAA‑AT‑GAGAC‑TTCCACC‑ACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCA‑CGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAAT‑TCCACACCAAAATAATCTTCA‑ATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGC  >  NC_000913/3266513‑3266982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑