Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,277,507	A→G	13.3%	E58G (GAG→GGG)	yhaV →	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,507	0	A	G	13.3%	201.3 / 4.1	97	E58G (GAG→GGG)	yhaV	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (28/53);  new base G (10/4);  total (39/58)
Fisher's exact test for biased strand distribution p-value = 1.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAA  >  NC_000913/3277269‑3277738                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              aaaaataaaaatcgtttttgCTGGCATGGAGATAACAATTGATGATGATATGGGCGCCGAAAAATAAGGTATTTGCCAAAAAGGTTTAATGGTTG‑GCGTATATACCCTGACCCGTTTTTACAGGAAA‑CCACCGACTCTTTAGTTGCGAAATTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATGAGAGAAAGGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTgagga                                                                                                                                                                                                                                               <  1:669825/220‑1 (MQ=255)       aaagaaaCTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGACAATTATCAGAGAAAAGCCGCCCCAAAGTTATTGGCGGGT‑GTCCATAAAAGGAATTAGGAGCata                                                                                                                                                                                                                                          >  2:456766/1‑238 (MQ=255)        aagaaaCTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑ATCCCAAAAATGATTGAGGAGCgtaat                                                                                                                                                                                                                                        >  2:822325/1‑237 (MQ=255)          gaaaCTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCCTAAAATGAGTGAGGGGCATATCAc                                                                                                                                                                                                                                      >  2:800621/1‑239 (MQ=255)               tgggcgcgGGCGTGGACTTCAAAATTGAGGATTGTATTGGCGTCAAGGAATAAGGGATTTCCCAAAAAGGGTTAAGGTTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGTCGTGTTAGTTGCCGAAGTCGAGATATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCa                                                                                                                                                                                                                                 <  2:527812/235‑1 (MQ=255)                                  cAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GGCCATAAAAGGTTTTAGGGGGATATAACGGTCAAAccccggcccttttatac                                                                                                                                                                                                              >  2:98689/1‑224 (MQ=255)                                  cAAAATTGATGATGAGATTGGCGACGACGAATAATGGATTTCCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCAt                                                                                                                                                                                                               <  2:801600/238‑1 (MQ=255)                                  aaaaatggagggtggaaTTGGCGACTACGAATAAGGGATTTGCCACAAAGGGTTAGTGGTTG‑GGCTCTATATGCTCATCTCTGTTTTCAGAAAA‑CCTACACTGTTTTAGTTGCGGAATTCGAAAAATTAAAGGG‑AAAAGA‑CCCTTAAATTTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCAt                                                                                                                                                                                                               <  1:946610/222‑1 (MQ=255)                                        tgatgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCCTAAATGGGTTGAGGAGCATATCACGGTAAATCCATCATCACCGGCATTCCGTCa                                                                                                                                                                                                        >  2:107703/1‑239 (MQ=255)                                        tgatgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGGGATTGAGGGGCATATCACGGTCAATCCATACTCACCCGCATTACCTCa                                                                                                                                                                                                        >  2:342468/1‑239 (MQ=255)                                         gatgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTAATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGA‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAt                                                                                                                                                                                                       <  2:1227549/239‑1 (MQ=255)                                             atgaggtggggaacgaagaATAATGGTTTTTCCACAAATGGTTAAGGGTTG‑GGCGCTATATGCACATCCCTGTTTCCAGGAAA‑CCTACGGCGCTTTAGTTGCCGAGGTCGAGACATTAAGGGG‑AAAAGT‑TCCTAAAAATTAGCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGc                                                                                                                                                                                                    <  2:695262/226‑1 (MQ=255)                                             atgaGATTGGCGACGACGAATAATGTATTTACCAAAAGGGGTTAATGGTTG‑GGCGATATATGCTCACCCTTGTTTTCAGGAAA‑CCTGCGACGCTTTAGTGGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAATTTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCa                                                                                                                                                                                                   <  2:920991/239‑1 (MQ=255)                                              tgaGATTGGCGACGCAGAATAATGTATTTTCCAAAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTCCGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCa                                                                                                                                                                                                   <  2:168664/238‑1 (MQ=255)                                              ggagtttggggtatacgaATAATGTTTTTCCAACAAAGGGTTAAGGGTGT‑GGCGCTTTATGCTCACCCCTGTTGTAAGGAAA‑CCTCTGGCGTTTTAGTTGCCGAAGTCGAGACATTAAAGGA‑AAAGTA‑TCTTGAAAATCATCAGAGAAAGGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCa                                                                                                                                                                                                   <  1:189305/224‑1 (MQ=255)                                               gtgtttGGCGCCGTAGAATTATTGTTTTCCCAAAAGGGGTTAATGGTGG‑TGCCCTATATGCTACCCCTTGTTACCAGGAAA‑CCTACGACGGTTTAGTTGCCGAAGCCGAGACATTAAAGGA‑AAAAGA‑CCCTGAAAATTATCAAAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAg                                                                                                                                                                                                 <  1:431266/235‑1 (MQ=255)                                               gagaTTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCACAAAAAGATTTAGGAGCGAAACACGGGTAATACATCATCAACGGCCGTCCGGCAAGGGaaa                                                                                                                                                                                                 >  1:888360/1‑238 (MQ=255)                                                    tgggcaagaggaAAAATGGATTTTCCAAAAGGGTTTAATGGTTG‑GGGGTTTATTGCCCACTCAGTTTTTCAGGAAA‑ACTACGCCTGTTTAAGTCCCGAATGCGAGACATTAAAGGG‑AAAAGC‑CCCGGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGtt                                                                                                                                                                                            <  1:1186491/229‑1 (MQ=255)                                                    tGGCGATGACGAATAATGGTTTTCCCAAAAAGGGTAAATGGTTG‑GCCATTATATGCTCATCCTTGTTTTCAGGAAA‑CCTACGGCGCTTAAGTTGCAGAAGCCGAGACATTAAAGGA‑AAAAGA‑CCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGtt                                                                                                                                                                                            <  2:445113/239‑1 (MQ=255)                                                          ggagatatattGTATTTTCCAAAAAGGTTTAAGGGTTGTGGCG‑TATATTC‑CACACCCGTTTTTAAGAAACCCTACGACGTTTTACATGCCGAAGGCGAGACATTAAAGGG‑AAAAGC‑TCCAGAAAATTACCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGct                                                                                                                                                                                       <  1:1241981/232‑1 (MQ=255)                                                                         tttCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGGGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCCAGTCGCTAGGCGC‑TG‑GGG‑AAAATAAAGa                                                                                                                                                                    >  2:106146/1‑239 (MQ=255)                                                                          ttCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAATGATTGAGGAGGCAATAAAGGGCCATTCCTACTCACCGGGATTCCCGCAGGGACAGTAGCTAAGCTC‑TG‑GGC‑AAAATaaaa                                                                                                                                                                     >  2:858903/1‑236 (MQ=255)                                                                                              tG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCCTAAAGAGATTGCAGAGCATATCACGGGCAATCCCTCATCACCCGCCTTCCGTTCTGGCAAGTCGTTATGCGCATG‑GGA‑AAAA‑AAAAACATGT‑CC‑C‑CGGTAAAAATTgg                                                                                                                                            >  2:1170412/1‑239 (MQ=255)                                                                                              tG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGGGCATATCACGGGCCATCCATCATCACCGGCCTTCCGTCATGGCAAGTCGGTAGGGTC‑AG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑CGGGAAAAATTgg                                                                                                                                            >  2:1250172/1‑239 (MQ=255)                                                                                              tG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GGCCCTAAAGGGATTTAGGAGCATAACACGGTCAATCCATCATCACCGGCATTCCGTCCTGGCAAGTCGTTAGGCTC‑TG‑GGG‑AAAATAAAGACAGGG‑CA‑C‑GGGGAAAAATTgg                                                                                                                                            >  2:718573/1‑239 (MQ=255)                                                                                               g‑ggCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCCTAAAAGGAATGAGGAGCATATCACGGTCAATCCATCATAACCGGCATTCCGCCATGGGAAGACGTTACGCTC‑TG‑GGG‑AAAAACAAAACTGGG‑AACC‑GGGAAAAATTTgc                                                                                                                                            >  2:476982/1‑238 (MQ=255)                                                                                                 ggCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAATGATTGAGGAGCATATCACGGTCAATCCATTCTCACCGGCAATCCGTCCTGGCAAGTCGTTAGGGCC‑TT‑GGG‑AAAATAAATACTGGT‑CC‑CGGGGAAAAATTTGGt                                                                                                                                           >  2:136215/1‑239 (MQ=255)                                                                                                   cgcTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GGCCATAAAAGGATTTAGGAGGATTTCACGGTCACTCCTTCATCCCCGGCATTCCGTCATTGGAAGACGTTGGGATC‑TT‑GGG‑AAAATAAAGACTGGT‑GC‑C‑AGGTAAAATTTtgtgtt                                                                                                                                        >  2:81048/1‑237 (MQ=255)                                                                                                        tatGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTTCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TC‑GGC‑AAAATAAAAAACGGG‑AC‑C‑GGGGAAAATTTTGttgtgggct                                                                                                                                   >  2:271225/1‑231 (MQ=255)                                                                                                               accccTGTTTTCAGGAAA‑CCTACGAGGCTTTAGTCGCCGAAGCCGGAACATTAAAGGG‑AAAAGG‑TCCTATAAATTAGCGGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGAGTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGt                                                                                                                            <  2:906502/237‑1 (MQ=255)                                                                                                               aTCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGGA‑GTTCATAAAAGGAGTGTGGGGGCAATCAAGGGTAAACCATCATCACCCGCATTCAGTCATGGAGACACGGTAGGGGC‑TC‑GGACAAAAAAAAACCGGGG‑CA‑C‑GGG‑AAAATATTGTGCTGGtgggtaaggg                                                                                                                            >  1:1060007/1‑230 (MQ=255)                                                                                                                tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTTCATAAAGTGATTGAGGAGCATATCACGGTCAATTCATCATTCACGGCCTTCCGTCCTGGCAACACGTTAGAACC‑TG‑GGG‑AAAATAAACAATAGT‑TA‑C‑CGGGAAAATTTGCTGCTGGTCGTTTTca                                                                                                                             >  1:1020484/1‑236 (MQ=255)                                                                                                                tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCCTAAAGAGGTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCCTGGCAAACCGTTAGGGTC‑TG‑GGG‑AAAATAAAAACTGGT‑CA‑C‑GGGGAAAATTTGTGGCTGGCCGTTATCg                                                                                                                             >  2:56109/1‑237 (MQ=255)                                                                                                                tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATCAAAGGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAAGCTC‑TG‑GGA‑AGAATAAAAAATTGT‑CA‑C‑GGGGAAAAATTTGTGCTGGTCGCTATTGt                                                                                                                            >  2:227035/1‑238 (MQ=255)                                                                                                                tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAAAGATTGAGGAGCCAAACAACGGCAATCCATCATCACCCGCATTCCCTCCTGGGAAGTCGGTAGGCCC‑TG‑GGT‑AAAATAAAAAATGGG‑CA‑C‑GGGGAAAATTTTGTGGTGGTCGTTATctc                                                                                                                            >  2:1228898/1‑236 (MQ=255)                                                                                                                tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GGCCATAAAATGATTGAGGAGCATATCACGGGCAATCCATCATCACCGGCATTCCGTCATGGCAAATCGTTAGGCTC‑TG‑GGG‑AAAATAAAAACCGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGGTATCg                                                                                                                             >  2:428320/1‑237 (MQ=255)                                                                                                                tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑AGCCCTAAAATGATTGAGGAGCCTATCACGGTTAATCCATCCTCCACGGGATTCCGTCCTGGCCAGTCGTTTGGGTC‑TT‑GGG‑AAAATAAAGAATGGT‑CA‑C‑GGGGaaaaccgtattctttaaccaactccc                                                                                                                           >  2:1210174/1‑217 (MQ=255)                                                                                                                    tGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAATGATTGAGGGGCATATCACGGGCAATCCATCATTACCGGCATTCCGGCCTGGGAAGTCGTTTGGGCC‑TG‑GGG‑AAAAAAAAAAATGGTACC‑C‑GGGAAAAATTTGGGGcctgtcctttttaacat                                                                                                                         >  2:111695/1‑222 (MQ=255)                                                                                                                    tGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GGCCATAAAATGATTGAGGAGCATATTCCGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGGTTGGCTC‑TG‑GGA‑AAAAAAAAAACTGGG‑CA‑C‑GGGGAAAAATTGTGCCTGGTCGTTATCGTCtctt                                                                                                                       >  2:411923/1‑239 (MQ=255)                                                                                                                     gTTTTCAGGAAA‑ACTGCGACGTGTAAGTGGCCAAAGTGAAAAAATTAAGGGA‑AAAAGC‑ACAGAAAAATTAACAAAAAAAAGCCGCCACAAAGGTATTGGCGGTA‑GGCCAGAAAGTGATTGAGGAGCATATGACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCtcttc                                                                                                                      <  1:745652/239‑1 (MQ=255)                                                                                                                      ttttCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTTAGGGGCATATCACGGGCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCTGTAGGCTC‑TG‑GGA‑AAAAAAAAGACTGGT‑CA‑C‑GGGGAAAATTTGGTGCTGGTGTGTATCGTCccttct                                                                                                                     >  2:235274/1‑239 (MQ=255)                                                                                                                        ttCAGAAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCttt                                                                                                                   <  2:727694/239‑1 (MQ=255)                                                                                                                              aaa‑CCTTCTGCGGTTTTGTTGGCAAAGTCGCGAAAATAAAGGGAAAAAGA‑TCTTAAAAATAATAAGAGAAAGGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTta                                                                                                              <  1:1228898/239‑1 (MQ=255)                                                                                                                               aaatctgcgacgtgtgatggaccGATGTAGAGAAATAAAAGTG‑AAAAGA‑TGGAGAAAATTAAAGAAAAAAAGCCGACACAAAGTTATTGGAGGTA‑GTCCAGAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGt                                                                                                                <  2:261679/215‑1 (MQ=255)                                                                                                                                            tttAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGGCAAACCAACATCACCGGCAATCCGGCATGGCAAGTCGTTAGGGCC‑TG‑GGG‑AAAAAAAAAACTGGT‑CA‑C‑GGGGAAAAATTTGTGCTGGTGGTTATCGTCTCTTCTTTCTTTTTAGTGgaaaaagga                                                                                                >  2:305240/1‑235 (MQ=255)                                                                                                                                            ttaaGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTa                                                                                                                                                    <  2:1023653/184‑1 (MQ=255)                                                                                                                                             ttAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAAGGCTTTAGGGGCATATCACCGTCAATCCATCATCCCCCGCATTCCGTCATGGGCAGTCGTTAGGCCC‑TG‑GGT‑AAAATAAAAACTGGT‑CA‑C‑CGGTAAAATTTTGTGCTGGTGCTTTTAGTCTCTTCTTTCGTTTTAGGGCAAAACAGaa                                                                                               >  2:46945/1‑239 (MQ=255)                                                                                                                                                  tGCAGAAGGTGAGAAATAAAAGGA‑AAAAGG‑TCTGAAAAATTAGAAGGAAAAAGCCGCCACAAAGGTATTGGCGGTA‑GTCCATAAAGTTATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                          <  1:106146/239‑1 (MQ=255)                                                                                                                                                   gCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAATGATTGAGGGGCATATCACGGGCAATCCATCATCACCCGCATTCCGTCAAGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAAAAAAAACTGGT‑CA‑C‑CGGGAAAATTTTGTGGCGGTCGTTTTCGTCTCTTCTTTCGTTTTAGTGAAAAAAAGAAAGccat                                                                                         >  2:401172/1‑239 (MQ=255)                                                                                                                                                       aaggggagaatttAAGGGG‑AAAAGA‑TCCTGAAATATAACAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                          <  2:1253051/223‑1 (MQ=255)                                                                                                                                                        acgagaaacaaaaaaGGGAAAAAGT‑GCCTAAAAAGTAACGGAAAAAAGACGCCACAAAGTTATTGGCGGGT‑GTCCATAAAGTGATTGAGGAGCAAATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAtt                                                                                     <  1:800621/227‑1 (MQ=255)                                                                                                                                                              gaCATAAAAGGG‑AAAAGG‑TCATGAAAATTACAAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGgga                                                                               <  2:758157/238‑1 (MQ=255)                                                                                                                                                                 aTTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCAGCCACAAAGTTATGGGCGGTA‑GTGCATAAAGTGCATGAGGGGGATAAAACCCACAACACCTCATCAACGACCCTTCGTCTTGGGCAATCGTTTGGCCC‑AGCGGA‑AACAAAAACAC‑AGT‑GA‑C‑GGGGCAAATTTGGTTCTGGGCGCTTTCCGCTATTATTTTGTTAATGTGAaaaaaaaaaaaacctacgttgttgcagtt                                                                           >  1:1253051/1‑220 (MQ=255)                                                                                                                                                                      aGGT‑AAAAGC‑TCCGAAAAATTATAAAAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc                                                                      <  2:1240155/239‑1 (MQ=255)                                                                                                                                                                      aGGG‑AAAGGA‑CCCTGAAAATTGCGAAGGAGACGCCGGTACAAAGTTATTGGCGGTA‑GGCCAGAAAGTCAGTGAGGATCATATCACGGTCAATCCAGCAGCGCCGGCATTCCGTCATGGCAAGTCGTTAGGCGC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTGCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGTAc                                                                      <  1:698648/239‑1 (MQ=255)                                                                                                                                                                       agg‑AAAATATTCCTGTAAATTATAAAAAAAAGGCCGCCACAAAGTTATTGGCGGTA‑GTACATAAAGTGAATGAGGAGCATATCACGGTCAATACATCAACACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAATACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc                                                                      <  1:157233/238‑1 (MQ=255)                                                                                                                                                                                  tCCTAAAAATAACCAAAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAaca                                                            <  2:452305/239‑1 (MQ=255)                                                                                                                                                                                     tgaaatattaaagaaAAAACCCGCCACAAAGTTATTGGGGGTA‑GTCCATAAAGGGATTGAGGAGCAAATCAGCGTCAATCCATCATCACCGGCATTCCGTAATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACActc                                                         <  1:1250172/228‑1 (MQ=255)                                                                                                                                                                                       taatttgcaagaaAAACGCCGCCCTATAGTTAGTGGCTGGA‑GTCCAATAGTTTATTGAGGAGCATATACAGGTCAAACCATCATCACCGGACTTCCGGCATGGCAAGTCGTTGGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGATAAAGTCATCATTCTGGGATGGATGAACGACGCAATCActgtt                                                       <  1:822325/230‑4 (MQ=255)                                                                                                                                                                                       aaaaTTTACAGAAAAAAGCCGCCACAACGTTATTGGGGGTA‑GTCCAAAAAGTTATTGAGGTACATATCACGGTCAAGCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTg                                                       <  1:411923/239‑1 (MQ=255)                                                                                                                                                                                       aaaaTTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTg                                                       <  2:888360/239‑1 (MQ=255)                                                                                                                                                                                         attgaaaggaaaaagtcccccAAAAAGTTATTGGCGGTA‑GTCCATAAAGGTATTGAGGAGCATATCACGGTCAAGCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTgcg                                                     <  1:271225/220‑1 (MQ=255)                                                                                                                                                                                                ggaaaagaGCCGCCACAAAGTTAGTGGCGGTA‑GGCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCAACGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                              <  2:35882/232‑1 (MQ=255)                                                                                                                                                                                                caagagacggggccgaaaaGTTATTGGCGGTATGTCAGTAATG‑GAGTGGGGAGCAGGTTGAGGACAATCCCTCATCACCGCAAGTCTGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CC‑G‑GGATAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGAGCCCCTAc                                              <  1:1226396/223‑1 (MQ=255)                                                                                                                                                                                                agaaAAATGCCTCCAAAAATTTTATTGCGGTA‑GGCCATAACGTGATTGAGGAGCAAAACACGGTCAATCCATCATCACCGGCATTCCGTTATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGTTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                              <  1:1195701/239‑1 (MQ=255)                                                                                                                                                                                                agaaAAAAGCCGCCACAAAGGTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                              <  2:426826/239‑1 (MQ=255)                                                                                                                                                                                                aaaaaaagcccaccAGAAAGTTATTGGCGGTG‑GTCCATTAAGTGATTGAAGACCATATCACGGTCAATCCATCAACACCGGCATTCCGGCATGTCAGGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATCGATGAACGATGAAAACACTCTGCGCACCTAc                                              <  1:227035/227‑1 (MQ=255)                                                                                                                                                                                                            tctaaatgTTATTAGCTTTT‑GTCCAAAAAGTGATTGAGGATCTTGTCCAGGTTAGTACATCAACATCAGTAATCCGTTATTGCAAGTCTGTATGCAC‑GG‑GGA‑AACATAAAGGCTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTTGCTTCTTTAGTTATAGTGAAAAAGAGAAAGTAATCATTCTAGTATGGATGAACGATGACAACACTCTGCGCACCTACGGTAAAAAAACa                                  <  1:593562/232‑1 (MQ=255)                                                                                                                                                                                                            ccACAAATTTAGTGGCGGTA‑GTCCATAAAGTTGTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGGCTGGT‑CA‑C‑GGGTAAAATTTTGTGCTTGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                  <  2:311065/239‑1 (MQ=255)                                                                                                                                                                                                            ccAAAAAGTTTTTGGCGGTA‑GTCCAAAAAGTGATTGAGGAGCAGATCCAGGTAAATCCAACATCACCGGACTTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                  <  1:305240/239‑1 (MQ=255)                                                                                                                                                                                                            cacaaaaGTTTTTGGGGGTA‑TTCCAAAAAGGGTTTGGGGGTAATTTCCAGGTCAAACCAACAACACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                  <  1:56109/235‑1 (MQ=255)                                                                                                                                                                                                             caaaaaGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                  <  2:1020484/235‑1 (MQ=255)                                                                                                                                                                                                              acaAAGTTTGTGGCGGTA‑GTCCATAAAGTGATTGAGGGGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTGTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                                <  1:136215/239‑1 (MQ=255)                                                                                                                                                                                                              acaAAGTTATTTGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCCCGGTCAATCCATCATCACCGGCATTCCGTCATGGAAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCGCTTCTTTCGTTAGAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                                <  1:401172/239‑1 (MQ=255)                                                                                                                                                                                                              acaAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                                <  2:485986/239‑1 (MQ=255)                                                                                                                                                                                                              aacaatttATTTGCCGTT‑GCCCATAAATTGAGTGAGGACCATTTCACCGGCAATCCATCATACCCGCGATGCCGGCATGGCAAGGCTTTAGTGTG‑TG‑GGA‑AAAATAAAGCCCGGT‑CA‑C‑GGGTAAAATTTGGTTCTGGTCGTTATCGTCTCTTCTTTCTTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGTTAAAAAAACAGa                                <  1:858903/233‑1 (MQ=255)                                                                                                                                                                                                               caAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGAt                               <  2:1060007/239‑1 (MQ=255)                                                                                                                                                                                                                 aatttAGTGGCGGTA‑GGCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                             <  2:530624/236‑1 (MQ=255)                                                                                                                                                                                                                 aaGTTTTTGGCGGTT‑GTCCAATAAGGGATTGAGGGGCATATCACGGGTAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                             <  1:428320/239‑1 (MQ=255)                                                                                                                                                                                                                 aaGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                             <  2:537623/239‑1 (MQ=255)                                                                                                                                                                                                                    ttATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCt                           <  2:1038664/238‑1 (MQ=255)                                                                                                                                                                                                                     tATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCt                           <  2:58331/237‑1 (MQ=255)                                                                                                                                                                                                                                   ccATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTGCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                                <  2:1247036/219‑1 (MQ=255)                                                                                                                                                                                                                                   ccATAAAGGGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTTATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata                        <  2:1061843/227‑1 (MQ=255)                                                                                                                                                                                                                                     cttaaGTGATTTGGGGGGATATCGCGGGAAATGCATCATAACCGGGATTCCGTCATGGCAAGGTGGTAGGGTC‑TG‑GGA‑AAAATAAAGGCTGGT‑AA‑A‑GGGTAAAATTTGGTGCTGGTCGGTAGCGTCACTTCCTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCGGCGCACCTACGGTAAAAAAACAGATGCCTATCACGTATTCAGCaaa          <  1:356710/236‑1 (MQ=255)                                                                                                                                                                                                                                     aTAAAGTGATTGAGGAGCATATCCCGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCaaa          <  2:125034/239‑1 (MQ=255)                                                                                                                                                                                                                                     aTAAAGTGATTGAGGAGCATAACACGGTCAATCCATCATCACCGGCATTCCGGCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCaaa          <  2:640612/239‑1 (MQ=255)                                                                                                                                                                                                                                       aaaGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAAt        >  2:721720/1‑239 (MQ=255)                                                                                                                                                                                                                                         aGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACCAGATGCCTATCCCGTATTCAGCAAAATGt      >  2:213305/1‑239 (MQ=255)                                                                                                                                                                                                                                           tGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCATCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                             <  2:511664/214‑1 (MQ=255)                                                                                                                                                                                                                                            gATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaa   >  2:923943/1‑239 (MQ=255)                                                                                                                                                                                                                                             aTTGAGGCGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCACGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGTGAAAATTTGTTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCCTCTTTCTGGGATGGATGAACGCTGAAAACACTCTCTGAACCTAAGGTAAAAAAACAGATGCCTATACCgccgactctaaaaccaacat  >  1:1247036/1‑220 (MQ=255)                                                                                                                                                                                                                                             aTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑CAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGGTATCGTCTCTTCTTTCGTTGTACGGAAAAACAGAAAGTCATCATTCTGGGATGGATGAACGGTGAAAACCCTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTACGCAAACAGTTaaa  >  1:1166723/1‑239 (MQ=255)                                                                                                                                                                                                                                             aTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaa  >  2:835443/1‑239 (MQ=255)                                                                                                                                                                                                                                             aTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGCCATAATTCTGGGATGGATGAACGATGAAAACACTCTTCGAACCTCCGGTAAAAAAACAGAGCCCTTAACCGAATTCAAAAAAAGTTTaaa  >  1:657330/1‑239 (MQ=255)                                                                                                                                                                                                                                             aTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGGTAAAAAGGGAAAGTCATCATTCTGGGATGGTTGAACGATGAAAACACTCGTCGCACCTACGGTAAAAAAAAAAGAGCCTAAACCGTATTCAGAaaaaattaaaa  >  1:1246008/1‑232 (MQ=255)                                                                                                                                                                                                                                             aTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑AA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCAGCATTCTGGGATGGTTGAAGGATGAAAACACTCTGCGACACTACGGTAAAAAAACAAAAGACTATACAGAATTAAGcttgccacttac  >  1:1061843/1‑228 (MQ=255)                                                                                                                                                                                                                                              ttGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGGTaaa  <  2:1215684/238‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              ACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTG‑GGCGCTATATGCTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGG‑AAAAGA‑TCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTC‑TG‑GGA‑AAAATAAAGACTGGT‑CA‑C‑GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAA  >  NC_000913/3277269‑3277738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑