| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,280,463 | T→A | 16.4% | C83* (TGT→TGA) | agaV → | N‑acetylgalactosamine‑specific enzyme IIB component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,280,463 | 0 | T | A | 16.4% | 145.0 / 2.4 | 62 | C83* (TGT→TGA) | agaV | N‑acetylgalactosamine‑specific enzyme IIB component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (42/8); new base A (3/8); total (46/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
AGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATC > NC_000913/3280233‑3280698 | aGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAaaac > 2:62343/1‑238 (MQ=255)aGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTCCTGGTTTG‑T‑AAAaccc > 2:1021845/1‑236 (MQ=255) gATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACccgccg > 2:645955/1‑239 (MQ=255) gATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACccgccg > 2:372148/1‑239 (MQ=255) gATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGAAGGAAATGGTA‑CTTGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAGAGTGTCGAGAGCATTCAACGCGCGCCCGACGGACAGAAAAACAGCCAGGTTTG‑A‑AAAAAACcccccg > 1:910987/1‑239 (MQ=255) cGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGAACACCAAAACCTGATGGAAATGGTA‑CGTGC‑AGAAGGGATCGCCGTACGGTTCTGG‑ACGCTGCAAAAAGGTATAGACAACCTTAATCGCGCCGCCGATAGACCGAAAATCCTCCTGGTTGGAA‑AAAACAcccccactttcctagc > 1:955239/1‑226 (MQ=255) gATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAAAACCCGCCGATTTCCTGACGCTgg > 2:611671/1‑239 (MQ=255) aTTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTCGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑GGAAGGGTTAGCCGGACGTTTCCGG‑ACTCTGCAAAAAGTTATCGAAAACATTCCGAGTGCCGCCGCTCGACAGAAAATCCGGCTGGTTTG‑T‑AAAACACCCCCCGAGTTCCGGatccgggg > 1:952793/1‑232 (MQ=255) gCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCCCCGCCGATCGACAGACAATCCTGCTGGTTTG‑T‑AAAACACCCCCCGATTTCCTTACGCGGGTGAAAGGGGGCGTTCCGGGGAACCGCATTAACggggg > 1:1280856/1‑235 (MQ=255) gttGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACTTGCATCGCGCCGCCAATCGACAGAAAATCATGCTGGTTGG‑G‑AAAACACACCCGGATTTACTGAAGCTGGTGAAGCGTGGGTTTCAAGGGAATCGCATTAAcctggcacaaatgacaaaa > 1:43745/3‑220 (MQ=255) tGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGcc > 2:448844/1‑239 (MQ=255) tGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGcc > 2:831674/1‑239 (MQ=255) tGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑CCGCCAATGGCAAAcaacaa > 2:917869/1‑238 (MQ=255) ggggttggcgaaagtcccGGTACAAAAAACCCTGA‑GGAAAGGGTACCGGGC‑AAAAGGGACCCCCGTACGTTTCGGG‑CCGCAGAAAAAAGTTATCGACAACATTCATCGGGCCGCCGATCGAAAGAAAATCCTGCTGTTTTG‑A‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAAcaacaa < 2:1117022/222‑1 (MQ=255) ggggtgccaaaaGATCCGGTAAAAAAAACCGTGATGG‑AATGGTT‑CCGGCAAGAGGGGATCGCCGTCCTTTTCGGG‑ACGCTAAAAAAGTTTATCGAAAACATCCACCGCCCCGCCGACCGAAAGAAAACCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAAt < 2:492458/229‑1 (MQ=255) ccGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCaaa > 2:842864/1‑239 (MQ=255) tCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAAGGGCAACCAACAAATCGCCAAAACGGttt > 2:234801/1‑239 (MQ=255) ccgggTAAAACAAAACCTGAGGAAAAGGGTT‑CGGGA‑AAGAGGGACCGCCGTCCTTTTCTGG‑ACGCGACAAAAAGTTACCGACAACATTCATGGCGCGGCCGATCGACAGAAAATCCTGTGGGTTTG‑T‑AAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGt < 2:1133188/234‑1 (MQ=255) tACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGGTTG‑T‑AAAACCCCCGCCGATTTCCTGACGCTGAGGAAAGGTGGCGTTCCGGTGAATCGCCTTAACCTAGGCACAATGCACT‑AAGCCCATGGCAAACAACAAACGGCCAAAAAGGTTTCtggg > 1:1089786/1‑237 (MQ=255) caaAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGCAAGGCGGCGTTCCGATGAATCGCCTTAACGTTGGCAATATGCACT‑ACGCCAAGCGAAAAAAACAAAACCCAAAAAAGGTATCtggagaagg > 1:581081/1‑232 (MQ=255) aaCCTGATGGAAATGGTA‑TTGGC‑AGAAGGGATCGCCGTACGTTTCTGA‑ACGATGCAAAAAGTTATCGACAACATTCATCGAGCCGCCGATCGACAAACAACCCTGATGGTTTG‑T‑AACACACCACCCGATTTCCTGCCACTGTGGAAACGCGGCGTTCCGTGGAACACCATTAACGTTGGCAATTTCCACT‑AACACAAGGTCAAAAAAAAACTCCCCAAAACGCTTTCtgtgaagtaggg > 1:95779/1‑231 (MQ=255) aaCCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATgg > 2:991097/1‑200 (MQ=255) ccTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACCCCCGCCGATTTCCTGACGCTGGTGAACGGGGGCGTTCCGTGGATCCGCATTAGCGTT‑GCACAATTGACTAACGCCAATGCAAAACAACAAAACGTACAAAAGCGTACTGGGGA‑T‑GCggggg > 1:692538/1‑237 (MQ=255) gATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGata > 2:382219/1‑239 (MQ=255) gATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑A‑CACACACCCGCCGCTTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCCCATTAACGTTGGCAATATTGCCT‑ATGCCAAGGGCAAAAAACAAATCACCAAAACgggtctttgagagtacggaggat > 1:516729/1‑218 (MQ=255) aaaaTGGTA‑CTGGC‑AGAAGGGACCCCCGTACTTTTCTGG‑CCGCTGAAAAAATTTATCAAAAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCg < 2:910987/237‑1 (MQ=255) aTGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCccagc > 2:1006591/1‑239 (MQ=255) aTGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCGGGTGAAAGGTGGCGTTCCGGTGAATCCCATTAACGTTGGCAAAATGCACT‑ACGCCAAAGGCAAACAAAAAATAGCCACAAAGGTTT‑TGTgggaacgcgggaaaactgaagc > 1:1131837/1‑219 (MQ=255) aTGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGAGCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCCGGTCAAAGGTGGCGTTCCGGTGAACCGCATTAACGTTGGCAATAGGAACT‑ACGCCAATGGCAAAAAACCAAACGCCAAAACGGTTTCTGGAGT‑G‑GCGGGAGATAACacaga > 1:1289100/1‑238 (MQ=255) gC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGATTCTGTGGA‑T‑GCGGGGGAAATCgaaaaattaaaagc > 1:249078/1‑226 (MQ=255) gAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGAAGCATTCA‑CCGACCt > 2:505440/1‑239 (MQ=255) ccccgtgcgttCTGGAACGCAGAAAAAGTTTATGGAAAACTTTCATGCCGCGGGCGATCGACAAAAAACCCGCCTGGTTTT‑A‑AAATCCTCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCg < 2:67196/230‑1 (MQ=255) tACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTggg > 2:186789/1‑239 (MQ=255) aCGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCAAATGGCAAACAACAAATCGCAAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTgggg > 2:1022077/1‑239 (MQ=255) tCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGATTG‑T‑AAAACACCCTCCGATTTCCTGACGCTGGCGAAAGGTGGCGTTCCGTCGATTCGCATTATCGTTGGCAATATGCAAT‑ACGCCAATGGCAAACAACACATCGCCAACACGGTTTTGGTGCA‑G‑ACGGGCGCTATCTCAGCTCTTA‑AACACCTTAAAACCCCAGGggaggac > 1:536126/1‑238 (MQ=255) g‑ACGCTGCAACAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTCCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGGAAATCGCATTAACGTGGGCAATATGCACT‑ACGCAAATGGCAAACAACAACTCGCAAAAAGGCATTCTGCGCG‑T‑GCGACCTATATCGAACCATTTA‑ACGACCTGCAACCCACTgggagggcagctt > 1:1008586/1‑229 (MQ=255) g‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTACTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCCCATTACCGTTGGCAATATGCACT‑ACGCCAATGGCCAACAACAAGTACCCAAAACTGTTTCT‑TGGACA‑CCGGGCCGTCTCGAGGCATTTA‑CCAACGTGAACACCTGTGGGGCGGatt > 1:169039/1‑234 (MQ=255) tGCAAAAAGTTATCGACAATATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGATTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGCATGGGGCCGTTCCGGTGAATCGCATTAACGTTGGCATTATGCACT‑ACCCCAATGGCAAACAACCAATCGCCAAAACGGTTT‑TGTGGG‑TACCGCGCGATCTCGCA‑CACTGACACGACCTGAAAACCCCCGGGGTGGAACGCttagat > 1:558949/1‑235 (MQ=255) tGCAAAAAGTTATCGACAACATTCATCGCGCCTCCGATCGACACAAAATCCTGCTTGTTTG‑T‑AAAACAACCACCGATTTCCTGACGCTGGTGAAAGGAGTCGTTCACGCGAATCGCATTACCGTTGCCAATAGGACAT‑ACGCCACTGGCAAACAACAAACAGCCCAAACGGCTTCTGTCGA‑T‑GCGCGCGATATCGAAGCATTTC‑ACGCCCCGAAAACAGACGGGGGGGaaagtgtcgtg > 1:739790/1‑230 (MQ=255) tGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AATACACCCGCCGATTTCCTGACGCTGGTGCAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATAGGCACT‑ACGCCAATGGCAAACAAGCAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGCAAACCGCTGGGGTGGGATGCTCCGtt > 2:815801/1‑239 (MQ=255) tGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCCATATGCACA‑ACGCCAATGGCAAACAACAAATACCCAAAAAGGTTTATGTGGA‑T‑GCGGCGGATATCACACCATTTA‑ACCACCCGCAAAACCCTGGGGTGGaaggactctt > 1:324018/1‑230 (MQ=255) tGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTTTGGA‑T‑GCGGGCGCTATCGCAGCATTTA‑ACGACCTGAAAAACCCTGGGGGGGAAGGCTTCGt > 1:61910/1‑238 (MQ=255) tGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGt > 2:139861/1‑238 (MQ=255) tGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGAAGAATTTA‑ACGACCTGAAAACCGCTGGGGGGGAAGGCTTCGt > 2:917079/1‑238 (MQ=255) tGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGAGAAAGGTGGCGTTCCGGTGAATCGCATTATCGTTGGCACTATGCACT‑ACGCCAATGTCGACCAACAAATCGCCACAACGGCTTCTGTGGA‑T‑GCGGTCGATAGCGCACCATTTA‑CAGACCACAAAACCACTGGGGGGGGATGCTTCGt > 1:405266/1‑238 (MQ=255) aaaaatttttaacaaccgtcccccgccCCGCCGAGCGAAAGAATGTCGTGGTTGTTTG‑A‑AAAACCGCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAg < 1:139861/216‑1 (MQ=255) tCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCt > 2:1259533/1‑222 (MQ=255) gccaccctctctCGCGCCGCCTCTCCAAAAACAATCCTGTTGTTTCT‑A‑AAAACGCCCGGCGCTTTCCTGACGCTGGTTAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GGGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGAc < 1:842864/228‑1 (MQ=255) atttCACCGGGCCGCCGATGGAAAAAAAATCTTGGGGGTTTG‑A‑AAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGc < 2:1127650/237‑1 (MQ=255) actcctccgccCCCCCGAGCAAAAGAAAATCCTCATGTTTTG‑A‑AAACCACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGc < 2:692538/232‑1 (MQ=255) cgcgCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACTGCCTGAAAACCGCGTGGGTGGAATg > 1:82306/1‑206 (MQ=255) cccgccgATCGAAAAAAATCCCGGGTGGTTTG‑A‑AAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGa < 2:1280856/238‑1 (MQ=255) gcgcagaagaagaGAAACTCCTGGTGTTTTG‑T‑AAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTACGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGAc < 2:785054/227‑1 (MQ=255) cggcgacgcaagaaaaaccctggggTTTG‑TAAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGAc < 2:558949/215‑1 (MQ=255) cgacggaaaaaaaaCCTTGCTGGTTAG‑A‑AAAACGCCCGCCGAATTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAACCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACctct < 2:43745/229‑1 (MQ=255) aaatggagagggttgGTTTT‑TAAAAACCCCCGGCGATGTCCTGAAGGGGGTGAAAGGGTGGGTGCCGGTGAAACGACTTAACCTTGGCAAGATGGCGT‑ACGGCAATGGAAAACAACAAATCGCCAAAACGGTTGCTGTGGA‑G‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAAt < 1:372148/226‑1 (MQ=255) aaaatttcttgttgttttG‑T‑AAAACCGCCGGCGATTTCCTGGCGCTGGTGAAAGGTGGCGTTGCGGTGAAACGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAAGCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGAc < 1:1232958/212‑1 (MQ=255) gAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTCTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACAACCTGAAAACCGCTGGGGGGGAATGCTTCGTTCAGGGCGTCCCGCCAGAGCCTCCTGTGGACCTCTTTAAAtt > 2:578885/1‑237 (MQ=255) tgtctagtgtgttG‑T‑AAACCCTCCGCCGATTGCCTTACGCTGGTGACAGGTCGCCATCCGGTGAATCGCATTAACTGTGGCAATATGATAT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGCCCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTg < 2:1089786/228‑1 (MQ=255) gctgGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑TCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGGATACTTCTTTCAGGGCGTCCCGACAAGGCCTGCTTTGGCCCTCTTTAAATTACTTTGAGGGa > 1:554857/1‑239 (MQ=255) gctgGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGGGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGGGGACCTCTTTAAAt > 2:1232958/1‑227 (MQ=255) ttG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATtcatc > 2:930741/1‑239 (MQ=255) | AGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTA‑CTGGC‑AGAAGGGATCGCCGTACGTTTCTGG‑ACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTG‑T‑AAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACT‑ACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGA‑T‑GCGGGCGATATCGCAGCATTTA‑ACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATC > NC_000913/3280233‑3280698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |