Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,289,008 C→A 9.0% S66* (TCA→TAA)  yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,289,0080CA 9.0% 316.8 / 2.1 117S66* (TCA→TAA) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (70/32);  new base A (2/10);  total (74/43)
Fisher's exact test for biased strand distribution p-value = 7.39e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CACTGCAATAAAAAATATAAAAACACAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAG  >  NC_000913/3288773‑3289241
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          
cACTGCAATAAAAAATATAAAAACACAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGAACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GAGGTATGAAAACAACCGATCTGTCGCGTTTTTaaaa                                                                                                                                                                                                                                         >  1:189169/1‑239 (MQ=255)
   tGCAATAAAAAATATAAAAACACAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GGGGAATGCAAACAACCGATCTGTCGCGTTTTTCaaaaaa                                                                                                                                                                                                                                      >  2:761148/1‑239 (MQ=255)
   tGCAATAAAAAAAATAAAAACACCGGCTATCAGGGAATGCCCCAA‑CG‑ACCCCCCCAGGGACAAAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCCCTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCaaaaaa                                                                                                                                                                                                                                      <  1:1204181/239‑1 (MQ=255)
     cAATAAAAAATATAAAAACACAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAat                                                                                                                                                                                                                                    >  2:26813/1‑239 (MQ=255)
       aTAAAAAATATAAAAACACAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTACCGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCAAAAAAA‑GTGGCATGCAAACAACCGATCTGTCCCGGTTTTCAAAAAAata                                                                                                                                                                                                                                   >  2:1233971/1‑238 (MQ=255)
          aaaaaaATAAAAACACAGGGCACAAGGGAAGGCCACAA‑GG‑ACTCCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCCTCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCAGGATTTGCACGACCGCTAACGCCGAAGAGTATTATTTCGACCTCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGca                                                                                                                                                                                                                               <  1:488282/239‑1 (MQ=255)
             actataAAAACACAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGGGTATTATTTCGACCCCATTATCCTGGAAACCACAAAAA‑GGGGTATGCAAACAACCGGACCGTCGCGGTTTTCAAAAAAaaataaccaa                                                                                                                                                                                                                            >  1:1248221/3‑233 (MQ=255)
             aaattaAAAACCCGGGTCACACGGGAAGCCCAAAC‑CG‑ACCCCACCAGGGACAGAAACGCAACCCGAAATCATTTGCCG‑TTATAATTAAACGTTGGTTGCCGAGGGGGCCACCGGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACaa                                                                                                                                                                                                                            <  1:943801/235‑1 (MQ=255)
             aaTTTAAAAACCCAGGTCACCAGGGAATGCCACAA‑CG‑ACCCCGCCAGGGACATAAACGGACCCCGGAA‑CAGTTGGCCCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTTCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCAGTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACaa                                                                                                                                                                                                                            <  1:305701/239‑1 (MQ=255)
             aaTAAAACACCACAGGTCACCTAGAAATCCCAAAA‑CGAACACC‑CCAGGGAAATAAACGCACACCGAAA‑CTGTTGCCGCTCATACTCAAACGTTGTTTGCCGAGGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGACGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACaa                                                                                                                                                                                                                            <  1:594039/239‑1 (MQ=255)
               tataAAAACACAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CACTTGGCGCTCATCATCAAACCTTGTTTGCCGATGGTGCCAACTGCCAGCGGCATGCTTTTCACTACCGCTAACGCCGAAGAGTCTTATTTCTACCCCTTTATCCCGGAAACCCCAACAA‑ACGGTAGTCAAAAAACCACTCTATCACACTTTTCaaaaaaaaaaaaaaaata                                                                                                                                                                                                                          >  1:102526/1‑228 (MQ=255)
                  aaaaaCGCAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTAcc                                                                                                                                                                                                                       <  2:783099/239‑1 (MQ=255)
                  aaaaaCCCAGGCCATCAGGGAATGCCACAA‑CG‑ACACCAACAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTccc                                                                                                                                                                                                                       <  2:1238718/239‑1 (MQ=255)
                  aaaaaCCCAGCGCACAAGGGATCGTCACAA‑CC‑ACGCCACCAGGGATACATACCCACCCCGAGA‑CAGTTGGCGCTCATCTTCAAGCGTTGTTTGCCGATGGTGCTCAATGGCATCTGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGGCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTAcc                                                                                                                                                                                                                       <  1:89018/239‑1 (MQ=255)
                          aGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑tt                                                                                                                                                                                                              >  2:905451/1‑239 (MQ=255)
                            gTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCACAAAAATATGCACAACTACCAGGAAC‑TTa                                                                                                                                                                                                             >  2:879446/1‑238 (MQ=255)
                             tcatcaGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACCCCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTAt                                                                                                                                                                                                            <  2:723136/238‑1 (MQ=255)
                                 caGGGAATGCCACAACCA‑ACACCA‑CATGGACATAAAATCACAACGAAC‑CAGTTGGCGCCTATCAACAAACTGTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATTCACAACTACCAGGAAC‑TTATCAGGt                                                                                                                                                                                                       <  1:107628/239‑1 (MQ=255)
                                 caGGGAATGCCACAA‑CG‑ACAACACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGAGGGTGCTCACTGGCAGCGGCACGCTTTGCACTACCACTAACGCCGACAGGAGTTATTTCGACCCCATTATGTGGGAAACAAAAAAAA‑GAGGGATGCAAAAAACCAATCGCTGACGTTTTTAAAAAAAATATGCACaaacaaaaagaacatatccagt                                                                                                                                                                                                        >  1:74800/1‑219 (MQ=255)
                                     gAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGt                                                                                                                                                                                                       <  2:1248221/235‑1 (MQ=255)
                                      aaTGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGata                                                                                                                                                                                                  >  2:26129/1‑239 (MQ=255)
                                      aaTGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAAAATGCCCAACTACCAGGAAC‑TTATAAGGTTgca                                                                                                                                                                                                   >  2:562453/1‑236 (MQ=255)
                                        tGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGCATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGTAAC‑TTATCAGGTTGAAATc                                                                                                                                                                                                >  2:649311/1‑239 (MQ=255)
                                           cacaA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAACA‑GTGGTATGCAAACAACCGATCGGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGACA‑TTATCAGGTTGata                                                                                                                                                                                                  >  2:1108658/1‑234 (MQ=255)
                                             caA‑CG‑ACACCACCAGGGAAATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCt                                                                                                                                                                                           <  2:228278/239‑1 (MQ=255)
                                                     caccacCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTAt                                                                                                                                                                                                            <  2:836793/216‑1 (MQ=255)
                                                              ggACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGAATGCAAACAACCGATCTGTCGCGTTTTTCCAAAAAAAATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGGTAAAAAAAAAAAAGGt                                                                                                                                                                            >  1:146291/1‑239 (MQ=255)
                                                               gACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCCCAAATACCAGGAAC‑CTATCAGGGTGaaa                                                                                                                                                                                                  >  1:836793/1‑214 (MQ=255)
                                                                  aTAAACGCACACCGAAA‑CGGTTGGCGCTCATCATCAAACGTTGGTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTc                                                                                                                                                                         <  2:762286/238‑1 (MQ=255)
                                                                  aTAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTca                                                                                                                                                                        >  2:914469/1‑239 (MQ=255)
                                                                     accGCTCACCTAAG‑CAGTTGGCGCTCATCATCAACCGTTGTTTGCCGATGGAGCTCACTGGCAGCGGCATCCTTTGCACTACCGCTAACGCCGACGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑ACGGTATGCAAACACCCGATCTGCCGCGTTTTTACAAAAAATATGCACAAATCACAGGAAC‑CTATCAGTTTTATATTTGTCTGGAACAAAAAAAGGATAcacaa                                                                                                                                                                     >  1:373191/3‑238 (MQ=255)
                                                                     aaCGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAg                                                                                                                                                                     >  2:292070/1‑239 (MQ=255)
                                                                       cGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGaa                                                                                                                                                                   <  2:146291/239‑1 (MQ=255)
                                                                         cacaCCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGCTATCTGGCTGATTAAAAAGAAGGTTTAACAGaaaa                                                                                                                                                                 >  2:120802/1‑239 (MQ=255)
                                                                         cacaCCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAAAAAAAAAAAGGTTTCaaaaaaaa                                                                                                                                                                 >  1:538775/1‑239 (MQ=255)
                                                                          acacCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTAcc                                                                                                                                                                                                                       <  2:352977/185‑1 (MQ=255)
                                                                          acacCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGCCGCGTTTTTCACAAAAATATGCACAACTAcc                                                                                                                                                                                                                       >  1:352977/1‑185 (MQ=255)
                                                                           cacCGAAA‑CAGTTGGCGCTCACCATCAAACGTTGTTTGCCGATAGCGCTCACTCGCAGCGACATGCTTTGCACTACCGCTAACGCCGAAGAGGATTATTTCGACCCCATTATGCTCGACACCACAAAAA‑GAGGTATGCAAACACCCGCATTGTCGCGTTTTTCCAAAAAATATGACCAACTACCAGAGAA‑TTAACAGGTTGCAAACCGTCTGAAAAAAAAAAAGGtttaccaagaaaaa                                                                                                                                                               >  1:286060/1‑228 (MQ=255)
                                                                                  a‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTGTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATACCTGGCTGAATAAAAAGAAGGTTTCACAAAAAAAAATTAAAt                                                                                                                                                        >  1:128899/1‑239 (MQ=255)
                                                                                       ttGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTa                                                                                                                                                     >  2:944524/1‑238 (MQ=255)
                                                                                       ttGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAAAAGGTTTTACAGAAAAAAATAAAATTTa                                                                                                                                                     >  1:1293079/1‑238 (MQ=255)
                                                                                                atcatcaAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGACCTGTCGCGTTTTTCAAAAAAATATACACAACAACCAGCAAC‑TTATCAGGTTGATATCTGCCTGCATAAAAAGAAGGTTTCACAGAAAAAAATTAAATTTACCCCCaacac                                                                                                                                           >  2:564354/1‑236 (MQ=255)
                                                                                                     caAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTCTTTCAAAAAAATATGAACAACTACCAGAAAC‑TTATCAGGTTGATAACTGGCTGAATAAAAAAAAGGTTTCACAGAAAAAAATAAAATTTACCGCCAATCCaaaac                                                                                                                                      >  1:286376/1‑235 (MQ=255)
                                                                                                            tgttTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACaa                                                                                                                                                                                                                            >  2:1150636/1‑147 (MQ=255)
                                                                                                            tgttTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACaa                                                                                                                                                                                                                            <  1:1150636/147‑1 (MQ=255)
                                                                                                            tgttTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACt                                                                                                                                  >  2:828620/1‑236 (MQ=255)
                                                                                                            tgttTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACAGCCAATCACGACCAACttat                                                                                                                               >  2:833389/1‑237 (MQ=255)
                                                                                                                tGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAACAC‑GTGGTATGCAAACAACCGCTCTGTCGCGTTTTTCAAAAACATATGCAAAACTACCAGGAAC‑TTATCAGGTTGATATTCGCCTAACTAAAAAAAAGGTTGCACAAAAAAAAATATCATTTACCGCCAAtcacaggcaacatcgcca                                                                                                                            >  1:967662/1‑221 (MQ=255)
                                                                                                                   cgtgggTGCCCACTGGCAGCGGCATGCTTGGCACTACCGCTAACGCAGAAGGATATTATTTCGACCCCATTATGCTGGAAACCAAAAAAA‑GTGGTATGAAAACAACCGATCTGTCGCTTTTTTCAAAAAAATATGCAAAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCca                                                                                                                        <  2:991030/235‑1 (MQ=255)
                                                                                                                    tttggtgcctccTGGCAGGGGCACTTTTTGCACTCCCCTTAACGACG‑TGAGTTTTTATTCCGCCCCCTTTTGCTGGAAACCAAAAAAAGGTTGTATCCAAACAGCCGTTCGTCCGTGTTTTTCAAAAAAATAAGCAAAACTACCGGAAAT‑TTAACAGTTTATTATCTGGTTAAATAAAAAAAAGTTTCCCCAAAAAAAAATTCCATTAACCGCCAATGCAGAGCAACTTCTGCAGCca                                                                                                                        <  1:73487/227‑1 (MQ=255)
                                                                                                                        gTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTATGCAGCCACAGtt                                                                                                                   >  2:123546/1‑239 (MQ=255)
                                                                                                                            ccACGGGCAGCGGCATGCTTTGCACTACCGCAAACGCCAAAGAGTATTATTTCGACCCCATTATGCTGAAAACCAAAAAAA‑GGGGTATGCAAACAACCGATCTGTCGCGTTTTTAAAAAAAATAGGCACAACTACCAGGAAT‑TTATAAGTTTGATATTGGGCTAAATAAAAAGAAGGTTTCACAAAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTAc                                                                                                                <  2:538775/237‑1 (MQ=255)
                                                                                                                             cACGGGCAGCGGCATGTTTTGCCCTACCGCAAACGCCGAAGAGTATTATTCCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACgg                                                                                                              <  2:201487/239‑1 (MQ=255)
                                                                                                                                   cAGCGGCATGCTCTGCACTACCCCTAACGCCGAAGAGTATTATTTGGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAAGTACCAGGAAC‑TTATCCGGTTGATATCTGGCGGAATAAAAAGAAGGTTTAACAGAAAAAAATTACATTTACCGCCAATGCAGGCAAACTTCTGCAGCCACAGTTTCCGAAAGaaa                                                                                                        >  1:1037968/1‑238 (MQ=255)
                                                                                                                                     ggggctggCTTTGCACTCCCGCAAAAGCCGAAGAGTATTATTTCACCCCCATTATGCTTGAAACCACAAAAA‑GTGTTATGCAAACAACCAATTTGTCGCTTTTTTCAAAAAAATATGCACAACTACCGGGAAT‑TTATCAGTTTGATTTCTGGCTAAAAAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGaaca                                                                                                       <  2:74800/231‑1 (MQ=255)
                                                                                                                                     gCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAACTATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAAATTTTGAAGCCACAGTTTACggaaaaaaac                                                                                                      >  1:774820/1‑231 (MQ=255)
                                                                                                                                     gCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGACC‑TTATCCGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCACATGCAGAGCAACTTCTTAAGCAACAAATTACGGAAGaaaaa                                                                                                      >  1:761414/1‑239 (MQ=255)
                                                                                                                                            gCTTTGCACTACCGCTAACGCCGAAGAGAATTATTTCGACCCCATTATGCTGGAAACCACAAAAC‑GTAGTATGCAAACAACCGATCTGTCGCGTTTTTCACAAAAATATGCACAACTACCAGGAAC‑TTTTCAGGTTGATATCTGGCTGAATAATAGAAAGGATTCACAGAAAAAAAATACATTTACCCCCAATGCAACACAACTTCTCCATCCAAAATTTAAGGTATaaaaacaaagta                                                                                               >  1:1107967/1‑233 (MQ=255)
                                                                                                                                              tttgcccccccGCCAACGGCGAAGATTTTTATTGCCACCCCTTTATTGGGAAAAACAAAAAGA‑GTGGTAAGAAAAAAACCGACCTGTCGCGTTTTTAAAAAAAATATCCACAACTACCAGGACT‑TAATCGGGTTAATATCGGCTTAAAAAAAAAGAAGTTTTCACAAAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAg                                                                                             <  1:26813/230‑1 (MQ=255)
                                                                                                                                              tgtgctcgaCCGATAACGCCGAGGAGTTTGTTTCCGCCCCGATTGTCATGAAAACAAAAAAAA‑CTAGTGTGCAACCATACTATCTTTCAGGTTTTTCAAAAAAATAGGCACATCTACGAGAAAT‑TAACGAGTTAAATATCTGTCTGAATAAAAAGAAGGTTTAACAAAAAACATTTACTTTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAg                                                                                             <  1:635803/231‑1 (MQ=255)
                                                                                                                                                ttccctccctcTAACGCCGAAGAGTATTATTTCGCCTCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCGATCGCGGTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATGTACCTCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt                                                                                           <  1:858770/229‑1 (MQ=255)
                                                                                                                                                tcccatACCTCAAACGCCAAAGGATATTATTTCGACCCAATTATGGTGAAAACCAAAAAAA‑GTGGTATGAAAACAACCGATCTGCCGCGTTTTTAAAAAAAATAGGCACAACTACCAGAAAC‑TTATCAGGTTGATATCGGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACTTTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt                                                                                           <  2:102526/234‑1 (MQ=255)
                                                                                                                                                tGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGGTTCACAGAAAAAAATTACATTTACCTCCAATGCAGAGCAACTTCTGCAGCCACAGTTTCCGGTAGAACAACTACGTGAgat                                                                                           >  2:1294232/1‑237 (MQ=255)
                                                                                                                                                tGCACTACCGATAACGCCGAAGAGTTTTATTCCGACCCCATTTTGCTGGAAACCAAAAAAA‑GGGGTATGCAAACAACGGTTCGTCCGCGTTTTTCAAAAAAATATGCACACCTACCAGGAAC‑TTATCAGGTTGATATCTGGCGAAAAAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt                                                                                           <  2:25561/239‑1 (MQ=255)
                                                                                                                                                       gttgtataagagaaaggTATTTTTGCGACCCCATAATGCGGAAAACCACAAAAA‑GTGGTATGCAAACACCCGATCTGTCGCGTTTTCAAAAAAAATTGCCAAAACTCCCAAGAAC‑TTACCAGGTTGTTATTTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTAt                                                                                     <  2:1173348/222‑1 (MQ=255)
                                                                                                                                                       ccGCTAACGCCGAAGAGTATTTTTTCGCCCCCATTATGCGGAAACCCAAAAAAA‑GTGGTATGCAAAAACCCGATCTGTCGCTTTTTTCAAAAAAATATGCAAAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAAAAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTAtt                                                                                    <  2:1293079/239‑1 (MQ=255)
                                                                                                                                                            aaCGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACCGTTTACGGGAGAACAACAAAGGAGGCTGGGCATTAAggg                                                                               >  1:1140967/1‑238 (MQ=255)
                                                                                                                                                                 cGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACgg                                                                                                              >  2:681730/1‑203 (MQ=255)
                                                                                                                                                                         attattTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCg                                                                  >  2:324686/1‑239 (MQ=255)
                                                                                                                                                                                cGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCt                                                                                                                                                                                           >  2:159661/1‑111 (MQ=255)
                                                                                                                                                                                agACCCCATTATGCTGGAACCCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAA‑TTATCAGGTTGATATCTGGCAGCATAAAAAGAATGTTTCACAGTAAAAAATTACATTTACCACCACTGCAACGAAACTTCTGCAGCAAAAGTTTACGGTAGAACAACTACGTAACCTGCGATTTAAGGTGGATAAAAACCCgggcctt                                                            >  1:274374/2‑233 (MQ=255)
                                                                                                                                                                                  aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGTTCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGACTAAAAAGAAGGTTTCAAGGAATAAAATTCCATTTACCGCCAATGCAGAGTAACTTCTGAATCCACACTTTAAGGTAAAAAAACTCCGTAACCTGGGGATTAAGGGGGCTGAAAAccgcgggctgaca                                                         >  1:654394/1‑228 (MQ=255)
                                                                                                                                                                                  aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTTAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCgctg                                                            >  2:1121337/1‑236 (MQ=255)
                                                                                                                                                                                  aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCCGGTTGATATCTGGCTGAATACAAAGAAGGTTTCACCGAAAAAAATTACATTTCCCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGTTGGGCATTAAGGGGGATGAAATACCCgcgccgcct                                                         >  1:785888/1‑234 (MQ=255)
                                                                                                                                                                                  aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACTATTACCGCCAATGCAGAGCAACTTTTGCAGCCCCAGTTTACGGTAGAAAAACAACAGAAACTGGGCATTAAGGGGGATGAAAAACCGGCgctggag                                                         >  1:656089/1‑237 (MQ=255)
                                                                                                                                                                                  aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACtt                                                                                                                                 >  2:822789/1‑167 (MQ=255)
                                                                                                                                                                                  aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCgctggct                                                         >  2:1041730/1‑239 (MQ=255)
                                                                                                                                                                                  aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCgctggct                                                         >  2:282890/1‑239 (MQ=255)
                                                                                                                                                                                  aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCgctg                                                            >  2:86184/1‑236 (MQ=255)
                                                                                                                                                                                      atttgtccTGGAACCCAAAAAAA‑GTGGTATACAAACACCCGATCTGTCGGTTTTTTAAAAAAAATATGCACAACTACAAGGAAC‑TTATCAGGTTGATATCTGGCTAAAAAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGaaa                                                     <  2:608135/232‑1 (MQ=255)
                                                                                                                                                                                       aTTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGGGGATGAAATCCCGGCGCTGGCTGaaaa                                                    >  2:1148574/1‑239 (MQ=255)
                                                                                                                                                                                       aTTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGATGAAATCCCGGCGCTGGCTGaaaa                                                    >  2:111588/1‑239 (MQ=255)
                                                                                                                                                                                               ggAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGGATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATAACg                                            >  2:905786/1‑239 (MQ=255)
                                                                                                                                                                                               ggAAACCAAAAAAA‑GGGTTAAACAAACTCCTGATCGGTCGTGTTTAACAAAAAAATAGTCAAAACTACCAGGATG‑TTAACAGTTTAATATCGCGATGAAAAAAAATGAGTTTCCACAGAAAAAATTTACATTTACCGCCAAGGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACg                                            <  1:828620/239‑1 (MQ=255)
                                                                                                                                                                                                gAAACCACAACAA‑ATGGTATGCAAACAACCGATCTGATGCGTTTTTGAAAAAAATATGCACAACTACAAAGAAA‑TTATCAGGTTGATATCTGAGTAACTCAAAATACAGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAATTTCGGCATCCACAATTTACGGCAGCACACCCATGTGCACTCGGTATTAAGGTGGAGGAAAGACCGACGCTAGTTGATAAAAAtagaaa                                           >  1:1195925/1‑234 (MQ=255)
                                                                                                                                                                                                     cacaAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCg                                      >  2:168983/1‑239 (MQ=255)
                                                                                                                                                                                                       aaaaaa‑GGGGTTGGAAAACACCCGATCTGTCGCGTTTTCAAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGTTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTg                                    <  2:774820/238‑1 (MQ=255)
                                                                                                                                                                                                               tgtaaTGCAAACAACCGATCGGTCGCGTTTTTCAAAAAAATATCCACAACTACCAGAAAC‑TTACAAGGTTGATATCGGGCGAAAAAAAAAGAAGTTTCCAAAAAAAAAAATTCCATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCaa                               <  2:289821/233‑1 (MQ=255)
                                                                                                                                                                                                                gtgaTGCACGCAACTGATCTGGCGCGGTTTTCAAAAAAATATGCACAACTACCAGGCAC‑TTATCAGAATGATATCTGACTGAATAAAAAGAAGGTTTCACAGAAAAAAATTAACTTTACCGCCCATGAAGACAAACTTCTCCAGCCAAACTTTCCGGTAACACACCTCCGTCGCCTGGGTATTACAGGGGATGAAATCCCCCCACAGGCGGAAAAAGagagtggagaccggataaacc                             >  1:705795/4‑218 (MQ=255)
                                                                                                                                                                                                                 gTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTc                            >  2:622229/1‑238 (MQ=255)
                                                                                                                                                                                                                   aTGAAAACACCCGATCTGTCGCGTTTTTTAAAAAAATATGCACAACTACCAGGAAT‑TTATCAGTTGGATATCTGCCTGAAAAAAAAGAAGGTTTCACAGAAAAAATTTACATGTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGc                          <  2:705795/238‑1 (MQ=255)
                                                                                                                                                                                                                           aacCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGCATTAAGGGGGCTGAAACCCCGGCGCCGGCTGAAAAAGAAGCCGAGAGCGTGTTAAACACCCTGGaaaaaa                 >  1:657943/1‑239 (MQ=255)
                                                                                                                                                                                                                                attgggcggtttttaCAAAAAAGAATCAAAAACTACAAGGACT‑TTATAGGGTTAATATCTGGCTAAAAAAAAAGAAGGTTTCACAAAAAAAAATTACTTTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCAtt            <  2:100878/229‑1 (MQ=255)
                                                                                                                                                                                                                                aTCTGTCGCGTTTTTCAAAAAAATATCCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGAAGCCACAGTTTACGTTAGAAAAACTACATGAGCTGGGGATTAAGGGGGATGAAATCCCGGCCCTGGCTGAAAAAAAGGACGAAAACGAGACCACATCCCTTGaaaaaacaat             >  1:919087/1‑234 (MQ=255)
                                                                                                                                                                                                                                 tttGTTGCTTTTTTCAAAAAAAATTGCCCAAAATCCAAGAACTTTACCAGTTTGATACCGGGCAAAAAAAAAAGAGGTTTTCAAAAAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCAtt            <  2:502302/237‑1 (MQ=255)
                                                                                                                                                                                                                                  tctgcgggTTTTTAAAAAAAAAGTGCAAAACTCCCAGGAAC‑TTATCGGGTTGATATCGGGTGAAAAAAAAAGAAGGTTTCAAAGAAAAAAATTAAATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTcc          <  2:1037968/232‑1 (MQ=255)
                                                                                                                                                                                                                                     tCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTTAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGCCGAAAGCGGGATCAACCAACGTGAACAAACAAATCCCgg       >  1:445917/1‑239 (MQ=255)
                                                                                                                                                                                                                                     tCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTGGCTTGAACAAATCATTCCCgg       >  2:175020/1‑239 (MQ=255)
                                                                                                                                                                                                                                       gcgTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATAATTCCCGGTa     >  2:50718/1‑239 (MQ=255)
                                                                                                                                                                                                                                          tttttAAAAAAAATATGCACAATTCCCGGGAAT‑TTACCGGGTTAATATCTGATAGAACAAAAAGAGGTTTTCAAAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAg  <  2:286060/239‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          
CACTGCAATAAAAAATATAAAAACACAGGTCATCAGGGAATGCCACAA‑CG‑ACACCACCAGGGACATAAACGCACACCGAAA‑CAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAAC‑TTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAG  >  NC_000913/3288773‑3289241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑