Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,499,649	G→T	15.9%	intergenic (+36/‑259)	yhfL → / → frlA	small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,649	0	G	T	15.9%	174.4 / 2.5	85	intergenic (+36/‑259)	yhfL/frlA	small lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (12/56);  new base T (12/3);  total (26/59)
Fisher's exact test for biased strand distribution p-value = 7.59e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

AGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTAT  >  NC_000913/3499435‑3499885                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTT‑CGGTCCTACTTCCCCGGAAGTTA‑TCTAC‑CAGAAA‑C‑GGCT‑ACAA‑A‑CCGG‑TTATTTTTCCCCACGCCCCCT‑G‑GATTTTCCg                                                                                                                                                                                                                            >  2:1143028/1‑239 (MQ=255) aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGGCCTACTTCACAGTAAG‑TT‑TCTTA‑CAGAAATC‑GGCG‑A‑AC‑A‑CCGG‑GTTAATTTTCCCACGCCCCCTCG‑GTTTTTcca                                                                                                                                                                                                                            >  2:163428/1‑238 (MQ=255)  gggAATGTAAATGAACAAATTTAGTAAAGGTGCCCTGGTGGGTGCAGTAATGGCTACGTGAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCGCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCg                                                                                                                                                                                                                            <  1:1033624/237‑1 (MQ=255)   ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTT‑CGGGCCTCCTCGACACTTAA‑TC‑TCTTACCAGAAAAC‑GCTA‑A‑AC‑A‑CCGG‑TTTGA‑TGCCCGCCACCCCCC‑C‑GAtttttcccgg                                                                                                                                                                                                                         >  2:10032/1‑233 (MQ=255)               aaCAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTC‑ACGTCCCACTTCCCCTGCAG‑TC‑TCTTG‑CAGAAAACGGGTT‑A‑AC‑A‑CCGG‑TTTAATT‑CACGACCCCCCCT‑G‑GGTTTTTCGCCGGCCCAATTGttt                                                                                                                                                                                                             >  2:1015498/1‑239 (MQ=255)               aaCAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTT‑ACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑TGGGCCCACTGCACCGACAT‑GC‑TCTTT‑CAGAGAAC‑GGGT‑T‑AC‑ACCCGG‑TTTTTTTGCCC‑ACCCCCTCC‑G‑GTGTTTTCCAGGGGGTGTttttttt                                                                                                                                                                                                            >  2:1201235/1‑238 (MQ=255)                    aTTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTACTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATg                                                                                                                                                                                                        <  2:789120/239‑1 (MQ=255)                                  cACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTGCTACTGCACAGTAAA‑TC‑TCTGA‑CCGAAAAC‑GGCG‑A‑AC‑A‑CCAG‑GTTAATTTTCCGGCGCCCCCT‑G‑GGATTTTCGCGGGGGTATTTGTTTTTATTTTcttc‑ttttccc                                                                                                                                                                                          >  2:316258/1‑233 (MQ=255)                                     tggttggtGCAATCTTGGCTTCTTTTACTGCATTGACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCcccgcc                                                                                                                                                                                    <  1:163380/239‑1 (MQ=255)                                       gTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTAATGGACAGGAAA‑TC‑TCTGG‑AAGAAATC‑GGCTCA‑AC‑C‑CCGG‑TTAATTTACCCAACCCCCCCT‑G‑GCTTTT‑CGAGGGCGGATTTGTTTCTTTGTTCTTC‑TT‑‑Tcccccccc                                                                                                                                                                                    >  2:383748/1‑237 (MQ=255)                                           gTGCAGTACTGGCTACTTTAACTGCATGTGCTCGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑aaa                                                                                                                                                                             <  2:223264/239‑1 (MQ=255)                                            tccagttttgtttgccttatttccATTTACTTGTCCTATTTGAAACCGTGATAAAAACTGCTCTTTCGACTACCTGCTGCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAAGC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑aaa                                                                                                                                                                             <  1:662072/215‑1 (MQ=255)                                            tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCctc                                                                                                                                                                                                                                         <  2:675011/184‑1 (MQ=255)                                                 tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑GGGTCCGTCCGCAAAGCAAA‑TA‑TCTCA‑ACGAAAAA‑TGCT‑A‑AC‑A‑CCCG‑CTTTACTGTCCGACCCCCTCC‑T‑GATGTTTCCAGGGGGTGTTTTCTTTTATGGACTTC‑TT‑‑Tccccccccacgcacaatatt                                                                                                                                                                        >  2:1014137/1‑227 (MQ=255)                                                 tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCCCACTAAA‑TC‑TCTGA‑CAGAAAAC‑CGCC‑A‑AC‑A‑CCCG‑GTTAATTTCCCGACGCCCCCC‑G‑GGTTTTTCCAGGGGGGATTTTTTTTTTTTTCCTTC‑TT‑‑TCcccccccccccaaaaaatt                                                                                                                                                                        >  2:582480/1‑228 (MQ=255)                                                    ttggtattttAACTTCATGTGCTGGTCATATTTAACACCCTTAAAAAACCTGTTTTTTCGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTc                                                                                                                                                                    <  1:109610/231‑1 (MQ=255)                                                      gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTT‑CGGGCCTACTGCTCCCTAAG‑TA‑TCTGA‑CAGAAATC‑GGCT‑T‑AC‑A‑CCCG‑TTTAATTTTCCCACGCCCTCT‑T‑GATTTTTCTAGGGCGCGTTTGTTTTTATGTCCTTT‑TT‑‑TCCCCCCCCCGC‑CAAAAAAttttt                                                                                                                                                                    >  2:172454/1‑236 (MQ=255)                                                      gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTT‑CGGGCCTACTGCCCCATCAA‑TCTTTTGA‑C‑GAAAAT‑GGCT‑A‑ACAA‑CCGG‑TTTATTTTCCCG‑CGGCCTCC‑C‑GGTTTTTTCAGGGCGGATTTTTTTTTTTTTGCCTC‑TT‑‑TTCCCCCCCCCT‑GAAAAAAttttt                                                                                                                                                                    >  2:1020531/1‑236 (MQ=255)                                                      gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGGCCTACTGCCCAGTAAG‑TC‑TCTTA‑CAGAAAAC‑GGGT‑A‑AC‑A‑CCGG‑ATTAATTTCCCGACGCACCCT‑T‑GGTTTTCCTAGGGGGTATTTTGTTTTTTGTCCTTC‑TT‑‑TCCCCCGCCCGG‑AAAAATAttttt                                                                                                                                                                    >  2:209270/1‑236 (MQ=255)                                                      gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGGCCTACTGCACAGTAAA‑TT‑TCTTA‑AAGAAATC‑GGCT‑A‑AC‑A‑ACGG‑GTTAATTTCTCGACGCCCTCT‑C‑GAGTTTACTGGGGGGTGTTTTTTTTTTTTTCCTTC‑TT‑‑TCcccccccccgcacaaaattttt                                                                                                                                                                     >  2:109610/1‑223 (MQ=255)                                                      gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCCGGCCTTCCTCCACACTAAG‑TA‑TTTGC‑CAAAAAT‑‑ACCT‑T‑AC‑A‑CCGGCTTCAATTGCCTGCCGCCCCCT‑G‑G‑TTTTCCTCGGGGGGACTTTTTTTTATTTGCTTC‑TTTCACCCCCCCCCAT‑AAAAAAAtttaa                                                                                                                                                                    >  1:716516/1‑237 (MQ=255)                                                                 tGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGa                                                                                                                                                                                                                                                <  2:799351/156‑1 (MQ=255)                                                                     tGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGGCCTACTGCACAGTAAA‑TC‑TCTGA‑CAGAAAAC‑GGCT‑A‑AC‑A‑CCGG‑GTTTATTTCCCGGCGCCCCCT‑G‑GAGTTTTCCAGGGCGTATTTGTTTCTATTTCCTTT‑TT‑‑TCCCCCCCCCCT‑CAAAAAATTTTTACCTTTA‑AATCAAtttt                                                                                                                                                  >  2:1075454/1‑238 (MQ=255)                                                                     tGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGGCCTAATGCACAATAAA‑AC‑TCTTA‑CAGAAAAA‑GGCT‑A‑AC‑ACCCGG‑TTTATTTTCCCACCCCCCCCT‑G‑GTTTTTCC‑AGGGCGCACTTTTTTTTTTTTCCTTCTTT‑‑TCCCCCCCCCCA‑AAAAAAATTTTCATTTTAA‑TTTAAAttt                                                                                                                                                   >  2:1006424/1‑239 (MQ=255)                                                                      gtccTGGTTATATTTAAAACCTTGATAAGAACTTGTCTTACGACTACCTGCTTCACCCGGCAATTTCTATTCTTAAAATCATTGGCGGTTG‑CGGTCCTACTGCTCAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGa                                                                                                                                                 <  1:172454/236‑1 (MQ=255)                                                                      gTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGCATACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGa                                                                                                                                                 <  2:174598/239‑1 (MQ=255)                                                                       ttgtggttactttcaaaaACGTGATAAGAACTGCTCTTACGATTTGCTGGTGCACCCGGCAATTTCTAATTCTAGAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CCGAACTC‑GGCG‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCCTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGAt                                                                                                                                                <  1:1014137/225‑1 (MQ=255)                                                                        acgggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGAt                                                                                                                                                <  2:806408/235‑1 (MQ=255)                                                                         cTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGCTT‑CTGTCCTCCAGCACAGGAAA‑TC‑TCTTA‑CACAAATG‑CGCG‑A‑AC‑A‑CCCG‑CTTTCTTTCCCTACCCCCCCT‑GCGATTTACTG‑CGGCGTATTTTTGTTTTTTTGCTTT‑TT‑‑TTCCCCCCCCGTCAAAAAATTTTTCACTTTAA‑Ataacatttttaa                                                                                                                                               >  1:517690/1‑228 (MQ=255)                                                                           ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGCTG‑TGGTCCTCCTGTAAAAGAAG‑AT‑TCTTT‑AAGAAAAC‑CGCG‑C‑AC‑A‑CCGG‑GTTTTTTGTCCGAACCCCTCC‑G‑GGGTTTTTCTGGGGGTGATTGTTTTTTTTGCCTTC‑TT‑‑TCCCCCCCCCCG‑CAAAACATTTTTTATTTAATATTAAAtttttcata                                                                                                                                             >  1:1247859/1‑233 (MQ=255)                                                                             tcttttttaaaaCCGTTATAAGAACTGCTTTTTCGGCTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGGGGGTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CATAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTAc                                                                                                                                          <  1:1061076/231‑1 (MQ=255)                                                                             tCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTg                                                                                                                                                  <  2:13920/231‑1 (MQ=255)                                                                              tattatGAAACCCGTTATAAGTACTTCTCTTACGCATACCTTCTGCACCCGGCAATTTCTATTTCATAAATCATTGGGGGTTG‑CGGTCCTCCGGCACAGTAAT‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑T‑CCGG‑TTTAATTGCCCGTCGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACGTTAA‑ATTCAATTTGATAACTAc                                                                                                                                          <  1:1285787/233‑1 (MQ=255)                                                                              cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTAc                                                                                                                                          <  2:705296/238‑1 (MQ=255)                                                                              cATATTGAAAACCGTGAGAAGAACTGCTCTTACGACTACCTGCTGCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTAc                                                                                                                                          <  2:726626/238‑1 (MQ=255)                                                                               attttGAAAACCGTGATAAGAACTGCTCTTACGACTTCCTGCTTCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACAt                                                                                                                                        <  2:984981/236‑1 (MQ=255)                                                                                 atTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTT‑CGGTCCTACTGCACAGGAAA‑TC‑TCTGA‑CAGAAAAA‑GGCT‑A‑AC‑A‑CCGG‑GTTTATTTCCCGACGCCCCCT‑G‑GATTTTCCCAAGGCGGATTTTTTTTTTTTTCCTTC‑TT‑‑TCCCCCCCCCCC‑AAAAATATTTTTACCTTTA‑ATTTAATTTGTTAACTACATc                                                                                                                                       >  2:1050161/1‑238 (MQ=255)                                                                                  cttaaaaccttgtttaagtcTGCTTTTACGATTGCCTGCTTCCACCGGAAATTTCTATTTCGAAAATCATTGGCGTTTG‑CGGTCCTACTTCACATTAAG‑TC‑TAGGA‑CAGAAATC‑GGAT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATGTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCa                                                                                                                                     <  1:1089814/220‑1 (MQ=255)                                                                                   tGAAAACCGTGAAAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCAt                                                                                                                                    <  2:665322/239‑1 (MQ=255)                                                                                         cctggtttataaCTTCTTTTTCGTCTCCCTTCTTCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  1:582480/229‑1 (MQ=255)                                                                                         ccGTTATAAGAACTGCTTTTACGACTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  1:1050161/239‑1 (MQ=255)                                                                                         ccGTGATAAGCACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  2:716516/239‑1 (MQ=255)                                                                                         ccGTGATAAGAACTGCTCTTACGACTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  2:411801/239‑1 (MQ=255)                                                                                         ccGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  2:968427/239‑1 (MQ=255)                                                                                            tGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGCTT‑CGGGCCTCCCGCACCGTAAT‑TC‑TCTTA‑CAGACATA‑GTCC‑A‑AC‑A‑CCCG‑ATTAATTGTCCGACGCCCCCT‑G‑GGTTTTTCGAGGGGGGATTTTCTTCTTTGTCCTTC‑TT‑‑TCCCCCCACCGT‑CAAAAAATTTTTTATTTTA‑TTTCATTTTTATAACAACAACCAtatcacaaaa                                                                                                                           >  1:1218877/1‑230 (MQ=255)                                                                                              ataagatctgattttctgctttccTGATGCACCCTGCAAATTATTGTTATAAAAGCATTGGCGGTTG‑CGGTCCTACTTAACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCTGAAGCCCTCT‑G‑GATTTTTCTAGGGAGTTGTTGTTTCTATGTCCTTC‑TT‑‑GACCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCCAAgcg                                                                                                                         <  1:453688/217‑1 (MQ=255)                                                                                              aTAAGAACCTCTCTTACGGCTTCCTGCTGCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAgcg                                                                                                                         <  2:573238/239‑1 (MQ=255)                                                                                                                  ctccTTCTGCCCCCGGCATTTTTTTTTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑C‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtg                                                                                                     <  1:163428/237‑1 (MQ=255)                                                                                                                   aCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  2:989036/213‑1 (MQ=255)                                                                                                                     ctgctgCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑C‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa                                                                                                  <  2:76138/239‑1 (MQ=255)                                                                                                                          gcgcccGGCAATTGCTATTTCTAAAATCATTGGCGGTGG‑CGGTCCTACTGAACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                                                                             <  2:1090993/236‑1 (MQ=255)                                                                                                                            aCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                                                           <  2:84416/239‑1 (MQ=255)                                                                                                                               cctctatttttttttataaatctaTTGGCGGTTT‑CGGTCCTACTGCACAGTAAG‑TC‑CTTTA‑CAGAAATT‑GGCT‑A‑AA‑C‑CCGG‑TTTAATTGCTCGACGCCCTAT‑G‑CATTTTCCGATGGCGTATTTGTTTCTATGTCCTTC‑TG‑‑TCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                                                                                        <  1:619430/216‑1 (MQ=255)                                                                                                                                 tatattttttttttaaaaaTTCTTGGGGGTTG‑CGGTCTTACTGCACAGTAAG‑TC‑TCCGA‑AAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                      <  1:1143028/224‑1 (MQ=255)                                                                                                                                  cAATTTCTTTTTCGAAAACTCTTTGCTGTTG‑CCGTCCTTCTGTACAGTAAG‑TC‑CCTGA‑CAGAAATC‑CGCT‑A‑AC‑T‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCTCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                      <  2:1249400/238‑1 (MQ=255)                                                                                                                                  cAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑C‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                      <  2:140420/238‑1 (MQ=255)                                                                                                                                  cAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                      <  2:663296/238‑1 (MQ=255)                                                                                                                                    atttctatttctAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTa                                                                                   <  2:622234/239‑1 (MQ=255)                                                                                                                                     tttctatttctaAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTAc                                                                                  <  2:696510/239‑1 (MQ=255)                                                                                                                                       tctatttctgAAAACATTGGCGGTGG‑CGGTCCCTCTGCCCAGTAAG‑TC‑CTTGA‑CAGAAATC‑GGGT‑A‑AC‑A‑CCGG‑TTTAATGGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTTTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATGAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                <  1:326131/239‑1 (MQ=255)                                                                                                                                        ttttttctaAAATCCTTGGACGTTG‑CGGTCCTACTGCCCAGTAAG‑TT‑TTTGA‑CAGAAACC‑GGCT‑A‑AC‑T‑CCGG‑TTTAATTGCCCGCCGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTGTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                               <  1:1201235/236‑1 (MQ=255)                                                                                                                                        ttattttgaAAATCATTGGCGGTTG‑CGGTCCTACCGCGCAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑C‑AC‑T‑CCGG‑TTTAATTGCCCGCCGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCACCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                               <  1:522538/238‑1 (MQ=255)                                                                                                                                          ttttctaAAATGATTGGGCGGTG‑GGGTCCTACTGAAAAAGAAG‑TC‑TCTTG‑CCGAAATC‑GGCT‑A‑AC‑A‑CCGG‑GTTAATTTCCCGGCGCCCTCT‑G‑GATGTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GACCCCGCACGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAAGTTTGTGATCTTCCTCCACATTACATAAc                                                                             <  1:10032/238‑1 (MQ=255)                                                                                                                                          atttctaAAATCATTGGCGGTTG‑CGGGCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑C‑CCTG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAc                                                                             <  2:1247859/239‑1 (MQ=255)                                                                                                                                              ctaAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatca                                                                         <  2:346053/239‑1 (MQ=255)                                                                                                                                                aaaaTCATTGGCGTTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                       <  1:383748/239‑1 (MQ=255)                                                                                                                                                  aattcttGGCTGTTT‑CGGTCTTTCTGCCCATTAAG‑TC‑GTTGA‑CAGAAATC‑GGCT‑A‑AC‑G‑CCGT‑TTTAATTGCCCGACGCCCTCT‑G‑GATTGTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCACGCCCGT‑AAAAATATTTTCCCTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                     <  1:1103518/234‑1 (MQ=255)                                                                                                                                                  aaTCCTTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCTGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                     <  2:415517/239‑1 (MQ=255)                                                                                                                                                  aaTCATTGGCGGTTG‑GGGTCCTTCTGTCAATGAAG‑GC‑TCTGT‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCTCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                     <  2:18129/239‑1 (MQ=255)                                                                                                                                                                g‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATtcatc                                                       <  2:1111543/239‑1 (MQ=255)                                                                                                                                                                     ttcatcTGCCCAGTAAT‑GT‑TCTGA‑CAGAAACC‑GGGT‑A‑AC‑A‑ACGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGTGTATTTTTTTCTATGTCCTTC‑TT‑‑GCCCCCGCACGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATg                                                    <  1:225534/233‑1 (MQ=255)                                                                                                                                                                       ttcctctggaacgttagttttgta‑CATAAATC‑GGTT‑A‑AC‑C‑ACGG‑TTTAATTTCCCGACGCCCTTT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCGCCCGCCCGT‑AAAAATATTTTCCCGTTAA‑ATTCAATTTGATAACTACATCCACTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATg                                                    <  1:513264/215‑1 (MQ=255)                                                                                                                                                                               caGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACTTACCATCATATGTTGTTATATTCATCATGCATTGTCAtt                                          >  2:513264/1‑237 (MQ=255)                                                                                                                                                                               caGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGt                                         >  2:488391/1‑239 (MQ=255)                                                                                                                                                                                 gTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGtt                                        >  2:767815/1‑238 (MQ=255)                                                                                                                                                                                  tAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGtt                                        >  2:376096/1‑237 (MQ=255)                                                                                                                                                                                           ctGA‑AAGAAAGC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGGCGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTAGGTCCTTC‑TG‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGGAA‑TTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTaaa                              <  2:739371/239‑1 (MQ=255)                                                                                                                                                                                            tGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtgtt                                                                   >  2:709321/1‑202 (MQ=255)                                                                                                                                                                                                aagcaaTC‑GGCT‑A‑AC‑C‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTGCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATg                            <  2:1079539/234‑1 (MQ=255)                                                                                                                                                                                                           cT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAAct                  <  2:230332/238‑1 (MQ=255)                                                                                                                                                                                                              a‑aC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGTGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACtctc               <  2:697150/239‑1 (MQ=255)                                                                                                                                                                                                                 tactccGG‑TTTAATTGCCCGGCGCCCTCT‑G‑GATTTTCCGATGGCGTTTTTGTTTCTATTTCCTTC‑TT‑‑GCCCCCCCCCGT‑AAAAATATTTTCCCTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTTTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACtctc               <  1:316258/235‑1 (MQ=255)                                                                                                                                                                                                                 atctccGT‑TTTATTTTCCCGAGCCCCTCT‑C‑TGTTTTCCGAAGTGTTATTTTGTTGTATGCTCTTT‑CT‑‑GCCCCCGCCCGT‑AAAAATTGTTTCCCTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACtctc               <  1:209270/235‑1 (MQ=255)                                                                                                                                                                                                                 aaccctgg‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACtctc               <  2:1053858/233‑1 (MQ=255)                                                                                                                                                                                                                               ttGCCCGACGACCTCT‑G‑GATTTTCCGAGGGCGTATTTGGTTCTATGACCTTC‑TT‑‑CCCCCCGCCCGT‑AAAAATCTTTTCACTTTAA‑AGTCAATTTGATAACTACATCCATTAGCCACCGCGCGATCCTTTGCGAAATTTTGTGCTCTCACATCACATTCCATACCATCCTATGTGGTTATATTAATATCGCCTTAGAATGTAACCTTTTAAATGAAGGCAAAATTGCCACTACataggat  >  1:516927/1‑233 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    AGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTG‑CGGTCCTACTGCACAGTAAG‑TC‑TCTGA‑CAGAAATC‑GGCT‑A‑AC‑A‑CCGG‑TTTAATTGCCCGACGCCCTCT‑G‑GATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTC‑TT‑‑GCCCCCGCCCGT‑AAAAATATTTTCACTTTAA‑ATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTAT  >  NC_000913/3499435‑3499885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑