Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,665,706	A→C	21.0%	V35G (GTT→GGT)	gadX ←	acid resistance regulon transcriptional activator, autoactivator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,706	0	A	C	21.0%	174.9 / 11.0	83	V35G (GTT→GGT)	gadX	acid resistance regulon transcriptional activator, autoactivator
Reads supporting (aligned to +/- strand):  ref base A (48/16);  new base C (3/16);  total (51/32)
Fisher's exact test for biased strand distribution p-value = 5.19e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAT  >  NC_000913/3665470‑3665923                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                              tACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAAcc                                                                                                                                                                                                                             >  2:134976/1‑239 (MQ=255)     tAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑Agg                                                                                                                                                                                                                         >  2:926960/1‑238 (MQ=255)        cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGCATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCa                                                                                                                                                                                                                      >  2:834577/1‑238 (MQ=255)        cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGCGATGAACGAGGAATGAAGAAACTTTTTT‑A‑TGT‑GAATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGGGCAAT‑AATGACA‑GCGAGTCCCTTCAA‑ACGTATATAAATTTTT‑C‑AAAAC‑C‑A‑TTCATAACTCGATTTTGCC‑TTGCATCCCCAAAAACA‑Agtaac                                                                                                                                                                                                                      >  1:722523/1‑234 (MQ=255)             cTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTCATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCTTCGGGAGAAAT‑ACTGAAA‑GCGTGTCCCTTGCC‑ACGAATACAAAATTTT‑C‑ACCAC‑A‑C‑TTATCTCATCGAATTTGGC‑TTGCATCCGCAAAAACA‑AGGACACccccc                                                                                                                                                                                                                >  1:51181/1‑238 (MQ=255)                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAACCC‑AGGCCACCGCCATTAAAATa                                                                                                                                                                                                        >  2:1252337/1‑239 (MQ=255)                             cTTATTTGGCAATTGTTGCCAAACTTCCGAAAAAAAGGG‑AATATCTCTCAAAAAGTAATGAAGGAGAAATGAAG‑AACTTTTTT‑T‑TGTGGAATGAAGTTTTAAT‑CCCTCCTCC‑TTGGGAATGGGGAAAAAT‑AATGAAA‑GCTTGTCCCTTGAC‑AAAAATACAAAATTTA‑C‑ACCAC‑A‑C‑TTATCTACTAGATTTTGGC‑TTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                <  2:357177/239‑1 (MQ=255)                               tATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAGGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGAGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑CGGCCAACGCCATTAAAATAGCGaaaaaca                                                                                                                                                                                              >  1:498476/1‑233 (MQ=255)                                    tGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTCCTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGAATCCGCACAAACC‑AGGTCACCGCCATTAAAATAGCGATCtttcccttt                                                                                                                                                                                         >  2:182347/1‑232 (MQ=255)                                    tGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGATTACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCGGCAAAAACC‑AGGTCACCCCAATTAAAAAAGGGAAAttaaacctt                                                                                                                                                                                         >  1:543555/1‑232 (MQ=255)                                    tGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACACAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAACAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCAtt                                                                                                                                                                                        >  2:1018220/1‑239 (MQ=255)                                      cGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTa                                                                                                                                                                                       >  2:733632/1‑238 (MQ=255)                                      cGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGAATTT‑CACATTTaa                                                                                                                                                                                      >  2:464688/1‑239 (MQ=255)                                            ttGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAAc                                                                                                                                                                                     >  2:365936/1‑234 (MQ=255)                                             tGCGCACTTTCCTGAAAAACGGT‑AATTTCTCTACAAAAGTGATGAACGAGTAATGAATAATCTTTTTT‑A‑TGG‑TAATGAAGATTTAAT‑CCCTCCTCC‑TTGAGCATCGGGAAAAAT‑AATGAAA‑GCTTTTCCCTTGAA‑AAAAATAAAAAATTTT‑C‑AACAC‑T‑T‑TTATCTACTCAATTTTGGT‑TTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGt                                                                                                                                                                               <  2:247198/239‑1 (MQ=255)                                              gcgcAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGCCACCGCCATTAAAATAGCGAAATT‑AACCATAAACCAAggga                                                                                                                                                                              >  1:1010255/1‑237 (MQ=255)                                              gcgcAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAAAGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAAAAGCACTATT‑AACCATTAACCATgggg                                                                                                                                                                              >  2:641902/1‑237 (MQ=255)                                               cgcaattgtatGAAAAACGGG‑AATTTTCTTACTAAGTTTATGAACGAGTAATAAAGAAACTTTTTA‑A‑TGG‑CCAAAAAATTTTAAG‑CCCTCCACC‑TGGAGCAGCGGGAAAAAT‑AATGAAG‑GGGTGCCCCCAAAC‑ACAAATAAAAAATTTA‑A‑AACAC‑A‑T‑TTATCTACCCAATTTTGGC‑TTGAATCCGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTga                                                                                                                                                                             <  2:943548/229‑1 (MQ=255)                                                gcAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGCA‑CCGAATACAAAATTTT‑C‑AACAC‑A‑A‑TTCACTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTAACCCCCATTAAAATAGCGATATT‑CACCATTAACCAGGGGgaa                                                                                                                                                                            >  1:1254492/1‑238 (MQ=255)                                                gcAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTgag                                                                                                                                                                            >  2:987784/1‑239 (MQ=255)                                                 acatttctaaaaaaaGAGA‑TATATCTCTCATAAAGGAATG‑ACGAGAAAAGAAAAAACTTTTTT‑A‑TGT‑GGAATAAAATTTTAT‑GCCCCCCCCTTTGAGCATGGGAAAAAAA‑AAGAAAA‑GCGTGTCCCTTGCC‑A‑GAAAAAAAAATTTT‑C‑AACCC‑C‑CTTTATCTACCCAATTTGGGC‑TTGCATCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTgaga                                                                                                                                                                           <  2:543555/229‑1 (MQ=255)                                                  catttgcaaaaaaaaAGA‑AATTCCTCTAATAAGGGGATGAACGAATAAAGAAAAATTTTTTTT‑T‑GGT‑GAAAGAAATTTTAAT‑GCCCCCCCC‑TTGAGCAGGGGGAAAAAA‑AAGAAAA‑GCGTTCCCCTTAAA‑ACAAAAAAAAAATTTT‑A‑AACCC‑C‑C‑TTTTCTACTCTATTTTGGC‑TTGCACCCAAAAAAACC‑AGGTCCCCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGaa                                                                                                                                                                          <  2:66081/230‑1 (MQ=255)                                                  aatttccaaaaaaaCAAG‑AATACCTCTACTCAGGGGAAGAAAGAATAAGAAAAAAATTTTTTT‑A‑TGT‑AAAATAAGATTTAAT‑CCCCCCTCC‑TTGACCATGGGAAAAAAT‑AATAAAG‑GCGTGTCCCTTGAC‑ACGAATAAAAATTTTA‑C‑ACCCC‑A‑C‑TTATCTACTCGATTTTGGT‑TTGCACCCACAAAACCC‑GGTTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGaa                                                                                                                                                                          <  2:358150/230‑1 (MQ=255)                                                   aatttcaaaaaaaaAGGAAATTTCCCTACGACATGGATAAACGAG‑AAAGAAAAAATTTTTTTGA‑GGT‑GAATGAAGATTAAGT‑CCCTCCCCC‑TGAAGCATCGGAAAAAAT‑AAGAAAA‑GCGTGCCCCTTGAC‑ACAAATACAAAATTTT‑C‑AACAC‑T‑C‑TTATCTACTCGAATTTGCC‑GTGCACCCGAAAAACCC‑AGATCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGaa                                                                                                                                                                          <  2:1255281/231‑1 (MQ=255)                                                    tttccaaaaaaaCAGG‑AATATCTCTACTGAAGTAAGAAACGAGTAATGAAAAAATTTTTTT‑A‑TGG‑GAAGAAAGTTTTAAT‑GCCTCCTCC‑TTGGGCAGCGGGAGAAAA‑AATAAAG‑GGGTGTCCCTTGAC‑CCAAATA‑AAAATTTT‑CAAACCC‑T‑C‑TTATCTACCCAAATTTGGC‑TTGCACCCGAAAAAACC‑AGGCCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAata                                                                                                                                                                        <  2:1136680/232‑1 (MQ=255)                                                    cTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAAGACAAAATTTT‑C‑AA‑AC‑ACC‑TTATCTACTGGAATTTGAC‑TTGAATCCGCCAAAACC‑AGCGCACCACCACTAAAATAGCAATCTT‑CACCacctgccccttaacaaca                                                                                                                                                                         >  1:83721/1‑221 (MQ=255)                                                        cTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATAtt                                                                                                                                                                    >  2:44077/1‑239 (MQ=255)                                                          aaaaaacaagatatattctcactacATTAATGAACGAGAAGTGAAGAATCTTTGTT‑G‑GGT‑GCATTAAGATCTACT‑CCCTCATCT‑TTGGCCAGAGTGAAAAAT‑AATGCAT‑GATTTCTCCTTGAA‑AGAAAAACAACTCTAT‑C‑ACCTC‑T‑C‑TTATCAACAAGAATTTGGT‑GTCCATCGGCAAAAATG‑AGTCCTCCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATAttt                                                                                                                                                                   <  1:733632/219‑1 (MQ=255)                                                             aaaCAGG‑AATCTCTCTACTACAGTTAAGAAAGAAAAATAAAAAAATTTTTTT‑A‑GGT‑GCATGAAGATTAAAT‑CCCCCCCCC‑TTGAACATGGGGAAAAAT‑AATGAAA‑GCGTTCCCTTTGAC‑ACAAATAAAAAATTTC‑C‑AACAC‑A‑C‑TTATCTACTCAATTTTGGC‑TTGCACCCGCAAAACCC‑GGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGt                                                                                                                                                               <  2:1010255/239‑1 (MQ=255)                                                                 aGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCtt                                                                                                                                                            >  2:12461/1‑238 (MQ=255)                                                                   g‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                                                                                                         >  2:236023/1‑239 (MQ=255)                                                                      atatCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑AACCATTAACCATGTTGAGAATATATTTATGCCTTGCAt                                                                                                                                                        >  2:170549/1‑238 (MQ=255)                                                                      atatCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATAAAAAATTTT‑A‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGAATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTACACATGGTGAAAAAACATTTATGTCttcaaa                                                                                                                                                        >  1:1104902/1‑234 (MQ=255)                                                                           tctACTACAGGGATGAACGAGTAATAAAGAATCTTTTTA‑A‑TGT‑GAAAGAAATTTTAAC‑CCCTCCCCC‑TTGAGCATCGGAAAAAAT‑AATAAAA‑GCGTTCCCCTTGAC‑ACAAATACAAATTTTC‑C‑AACAC‑A‑T‑TTATCTACTCAATTTGGGC‑TTGCACCCAAAAAAACC‑AGGTCCCCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGaa                                                                                                                                                                          <  2:665917/215‑1 (MQ=255)                                                                              actacAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCCTTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAAAATATTTATGTCTTGCAAACGAAAtaa                                                                                                                                               >  1:699988/1‑237 (MQ=255)                                                                                  cAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCATCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGTC‑TTGCATCCGCAAAAACC‑AGGTCCCCGCCATTACAATCGCGATATT‑CACCATTAACCATGGTGAGAATATATATATGTCTGGCAAAGGCAAATAGACa                                                                                                                                           >  1:650116/1‑239 (MQ=255)                                                                                   cgaggaagaagtaaaaTAAAGATAGTTTTTT‑T‑TGT‑GAATGAAGAATTAAC‑TCCTACGTG‑TTGATCCTGCGGAAAAAT‑AACAAAA‑GCGTTCCCCTTGAA‑CAGAAAAAAAATGTGG‑C‑AACAC‑C‑T‑TTTTTTGTGAGTATTGGGT‑TTGCCCCCAAAAAAACC‑AGGTACCCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                          <  1:926960/225‑1 (MQ=255)                                                                                    agtaatgaaagaaaaaaaaaaaaTTTTTTT‑AGTGG‑GAATAAATATTAAAT‑GCCTCCCCT‑TGGAGCAGGGGAAAAAAT‑AA‑GAAA‑GCGTGGCCCTTAAA‑ACAAAAAAAAAATTTTAC‑AACCT‑C‑T‑TTACCTATCAGATTTTGGC‑TGGCACCCGAAAAACCC‑AGGCCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                          <  2:1104902/219‑1 (MQ=255)                                                                                     tgatgaACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTACCCGCCATTAAAATAGCGATATT‑AACCATTAACCATGGGGAGAATATATTAATGTcattctcttaaacacatataac                                                                                                                                         >  1:665917/1‑217 (MQ=255)                                                                                     taagcactgaaaaaTGAAAAACTTTTTTT‑A‑TGT‑GCATGAATTTTTAAG‑CCCCCCTCC‑TGGAACATGGGGAGAAAT‑AATAAAA‑GCTTGTCCTTTGAA‑ACAAATAAAAAATTTC‑C‑AACCC‑C‑T‑TTATCTACTCAATTTTGGC‑TTGCACCCAAAAAACCC‑AGGCCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                          <  2:945130/225‑1 (MQ=255)                                                                                       atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCTCCATTAAAATAGCGCTATT‑CACCATGAACAATTGTGAGAATAAATTTATGTCTTGCAATCGCAATTAGTCAATTc                                                                                                                                       >  2:706516/1‑238 (MQ=255)                                                                                       atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATg                                                                                                                                                                >  2:410580/1‑213 (MQ=255)                                                                                       atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAcctgtct                                                                                                                                      >  2:859175/1‑233 (MQ=255)                                                                                       atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                       >  2:608394/1‑238 (MQ=255)                                                                                       atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                       >  2:1110994/1‑238 (MQ=255)                                                                                       atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                       >  2:1285586/1‑238 (MQ=255)                                                                                       atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAACATTATT‑CACCATTAACCATGGGGAGAAAATATTTATATATTGAATACGCAATTAAAAAATTcc                                                                                                                                      >  1:1226981/1‑239 (MQ=255)                                                                                       atgaACGAGAAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACAAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCAAATTTGGC‑TTGCATCCTCAAAAACC‑AGGTCACCGCCATTAAAAAAGCGATATT‑CACCATTAACCATGCTGGTAATATATTTATGTCTTTCATACGAAATTAGACAATTc                                                                                                                                       >  2:754674/1‑238 (MQ=255)                                                                                          accgagaaaaaaaaaaaCTTTTTT‑A‑TGG‑GAATAAAATTTTAAT‑CCCTCCTCC‑TTGAGCATGGGGAAAAAA‑AAAGAAG‑GCGGGTCCTTTAAC‑AAAAATAAAAAATTTC‑C‑AACCC‑T‑C‑TTATCTACTAAATTTTGGA‑TTGCACCCGAAAAAACC‑AGTTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                                                                                                   <  2:959030/225‑1 (MQ=255)                                                                                          aaCGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAAAAGCGCTATT‑CACCATTAACCATGGTGAGAATCTATTTATATCTTTCATACGCAATTAAACAATTCCCaa                                                                                                                                   >  1:1280265/1‑238 (MQ=255)                                                                                            cGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAACCC‑AGGTCACGGCCCTTAAAATAGCGCTATT‑CCCCATTAACACTGGTGAGAAAATATTCATTTCTTGCTAAAGAAATTAGACAATTCCAATga                                                                                                                                 >  1:913389/1‑238 (MQ=255)                                                                                               gTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑t                                                                                                                             >  2:1149030/1‑239 (MQ=255)                                                                                                   taaaaattttttttt‑A‑TTT‑ACATGATTATTTACC‑CCCCCCCTC‑TTCAGCATGGGGAAAAAT‑AAT‑AAG‑ATGTTCTCCATGACTACAAAAAAAAATTTTA‑C‑AACAC‑A‑C‑TTATATACAGTATTTTTGT‑TTCCATCAAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGAtt                                                                                                                         <  1:1285586/230‑1 (MQ=255)                                                                                                          tttttttt‑A‑TTG‑GCATGAATTTTTAAC‑CCCTCCTCT‑TGGAGCATGGGAAAAAAT‑AAGGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTCT‑C‑AACAC‑T‑T‑TTATCTCCTCGAATTTGGC‑TTGCATCCACAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGt                                                                                                                  <  2:699988/237‑1 (MQ=255)                                                                                                          tttttttt‑A‑TGT‑GATTGAAGTTTCACT‑CCCTCCCCC‑TTGAACATAGAGAAAAAA‑AAGAAAC‑GTTTGTCCTGTGAT‑CAAAATAAAAAAATTA‑A‑AACAC‑A‑C‑TTATCATGTAGATTTTGTA‑TGTGACCCGCAAAACCC‑AGTGCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGt                                                                                                                  <  1:134976/237‑1 (MQ=255)                                                                                                          aCTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGCATATATTTATGTCTTGAATAGGCAATTATACAATTCCAATGTAC‑GAATTCCATAGt                                                                                                                  >  2:1014665/1‑239 (MQ=255)                                                                                                             cttttttgaggggaataaaattttAAG‑CCCTCCGTC‑ATGAGCATGGGAAGAAAG‑AAAAAAA‑GTGTTCCCTTTGAC‑AGGAAAAAAAAATTGT‑C‑ACAGC‑A‑T‑TTATCTACCCGATTTGTGC‑TTGACCCAGAAATAACC‑GGGCGACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGt                                                                                                                  <  2:880558/218‑1 (MQ=255)                                                                                                                  tttattgacATAAATATTTAAT‑GTCCCCTCC‑TTGAGCAGCGGGAAAAAT‑AAGGAAC‑GCTTCCCCTTTGAC‑ACAAATAAAAATTTTA‑C‑AACAA‑T‑C‑TTATTTACACAAATTTGCC‑TTCACCCCGCAAACACA‑AGTTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACt                                                                                                             <  2:401604/231‑1 (MQ=255)                                                                                                                      gt‑gCATGCAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCGCTTGAC‑AAGAATACAAACTTTT‑T‑AACAC‑A‑C‑TTATCTACTCGAGTTTGGC‑TTGCATCCGCATAAAAC‑TGGTAACCCCCATTAACATAGCGCCATTACACC‑TTAACCATGGTGGCGATATATTCATGTCATCCATACGCAATACAACCATTGCAAAGTAC‑TGTTTGCATAGTTGACTaaaaa                                                                                                        >  1:271075/1‑237 (MQ=255)                                                                                                                         ggcaggaaaTTTAATCGCCTCTCCT‑TTGACCAT‑GGGAAAAATAAATAAAG‑GCGTGTCCTTTGAC‑ACAAATA‑AAAATTTT‑C‑AAAACTA‑T‑TTATC‑ACCTGAATTTTGG‑TTGCCACCCAAAAAACCAAGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAata‑t                                                                                                      <  2:947642/231‑1 (MQ=255)                                                                                                                         gCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATATCGATATT‑CACCATTACCCATGGTGAGAATTTATTTATGTCTTGCATACGCAATTAGACAATCCCCATGTAG‑Tgctgtctcttataaacaacaaaa                                                                                                      >  1:1134145/1‑217 (MQ=255)                                                                                                                             taaattttAAT‑GCCCCCTCC‑TGGAGAACGGGAAAAAAA‑AATGAAA‑GGGGGCCCCTTGAC‑AAAAATAAAAAATTTA‑C‑AACAC‑T‑C‑TTATCTACCCGAATTTGGC‑TTGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACAt                                                                                                 <  2:138100/234‑1 (MQ=255)                                                                                                                             gaatttttAAT‑CCCCCCTAC‑TTGAACAGGGGGAAAAAT‑AAAGAAG‑GCGTCTCCCTT‑AC‑AAAAAAAAAAAATTTTAC‑AACCC‑T‑T‑TTATCTCCTAAAATTTGGC‑TTGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACAt                                                                                                 <  2:1226981/234‑1 (MQ=255)                                                                                                                             aaataTTTACT‑GCCCCCCCC‑TGGAGCA‑GGGGAAAAAA‑AAAGAAAGGCTTTCCCCTAGAC‑AAGAAAAAAAATTTTC‑C‑ACCCC‑A‑T‑TTTTCTACTCTATTTGGGC‑TTGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACAt                                                                                                 <  2:249728/235‑1 (MQ=255)                                                                                                                               agagTTAAC‑GCCTCGTCC‑TGGAGCATGGGGAAAAAT‑AAGAAAA‑GTGGCTCCTTAGAC‑A‑GAAAACAAAATTTTCC‑AACAT‑A‑T‑TTATCTACTAGATTTTGGC‑TTCCTCCCAAAAAAACC‑AGGTAGCCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATaa                                                                                               <  2:239877/239‑1 (MQ=255)                                                                                                                                  tttAAG‑CCCCCCGCC‑GTGAACAACGGGAAAAAA‑AAGAAAG‑GCTTCTCCCTTGAC‑ACAAAAAAAAATTTTC‑C‑AACAC‑A‑C‑TTATCTACCCAATTTGGGC‑TTGCACCCGAAAAAACA‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACa                                                                                            <  2:18165/239‑1 (MQ=255)                                                                                                                                    tAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGACCCTTGAC‑ACGAATACAAACTTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCCTTAACCATGGTGAGATTATATTTATGTCTTGCATACGCAATCAAACAATTCCCAT‑TAGAGGTTTGCATAGtttcataaaaataaactaaaaaaa                                                                                           >  1:7115/1‑217 (MQ=255)                                                                                                                                       aagcccgccgcatagAGCAGGGGGAAAAAT‑AATGAAC‑GCGTTACCATTGCA‑ACAAAAAAAAAATTTC‑A‑AACAC‑A‑C‑TTATCAACTGAAATTGGGC‑TTGCATCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACatat                                                                                         <  2:51181/225‑1 (MQ=255)                                                                                                                                              ctcc‑tTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑t                                                                                                                             >  2:1230571/1‑196 (MQ=255)                                                                                                                                                 ccatgagatggggaaaaaaa‑aaGGAAA‑GCTTTCCCCTAAAC‑ACAAAAACAAATTTTT‑C‑AAC‑C‑C‑C‑TTAACTTTCCAAAATTGGCTTTGCATCAACAAAAACC‑AGGTCTCCGCCATTAAAATAGCGATATA‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTttat                                                                               <  2:498476/223‑1 (MQ=255)                                                                                                                                                          tCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAata                                                                                                        >  2:47405/1‑206 (MQ=255)                                                                                                                                                           cGGGAGAAAT‑AATAACA‑GCCTGTCCCTTGAC‑ACGCATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGACTTTGGC‑TTGCATCCGCCAAACCC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATCATCCATGTTGAGCATTTATTCATGTCTTGCATACGCAATTATACAATTCCCATGCAG‑TGATTGAATTGTTGATTTAAAA‑TTACATAAAAAAATACCTGTTATTTAAtatacc                                                                      >  1:218223/1‑236 (MQ=255)                                                                                                                                                            gggAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCCTGGTGAGAATCACTTTATGTCTTGCATACTCAATTAGACAATTCCCATGGAG‑TGATTGCATAGTTGAATTAATATTTACA‑AAAAAAATTTCTTTTTTTTAATGTAACa                                                                     >  1:868180/1‑238 (MQ=255)                                                                                                                                                                 aaaT‑AAAGAAA‑GCTTTCCATTTCAC‑ACAAAAACAAATTTCA‑C‑AACTC‑C‑C‑TTACCCACTCAAATTTGCC‑TTGCCACCCAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACTCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCAcgccgc                                                               <  2:315135/239‑1 (MQ=255)                                                                                                                                                                                                 aTACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAg                                                                                                                   >  2:610798/1‑159 (MQ=255)                                                                                                                                                                                                            t‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAAtt                                                                          >  2:504669/1‑187 (MQ=255)                                                                                                                                                                                                              c‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAg                      >  2:120650/1‑239 (MQ=255)                                                                                                                                                                                                                     ctcttttttccaCTCGATTTGGGT‑TTGCACCCAAAAAACCT‑AGGACACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCgg                   <  2:1214691/228‑1 (MQ=255)                                                                                                                                                                                                                          tatctcaccgaattttGCC‑TTGCACCCGAAAAACCC‑GGTTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGaa              <  2:868180/226‑1 (MQ=255)                                                                                                                                                                                                                               tatacaaatttgggccggCATCCAAAAAAACC‑GGGCAGCCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAgg          <  2:218223/222‑1 (MQ=255)                                                                                                                                                                                                                                  tCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGAGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TAATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCACCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACAAAACGGGAAAAAAgaggaa      >  1:1288753/1‑234 (MQ=255)                                                                                                                                                                                                                                      aTTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAt  >  2:1139546/1‑239 (MQ=255)                                                                                                                                                                                                                                            c‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACa            >  2:706131/1‑223 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                              TACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGT‑GCATGAAGATTTAAT‑GCCTCCTCC‑TTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑A‑C‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATT‑CACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAT  >  NC_000913/3665470‑3665923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑