Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,757,531 | G→A | 14.0% | S99F (TCT→TTT) | yiaY ← | L‑threonine dehydrogenase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,757,531 | 0 | G | A | 14.0% | 148.2 / 2.4 | 60 | S99F (TCT→TTT) | yiaY | L‑threonine dehydrogenase |
Reads supporting (aligned to +/- strand): ref base G (37/14); new base A (3/6); total (40/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.88e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCAT > NC_000913/3757298‑3757760 | tgTCATAGACAGAAAGGAGGGGGTTGACATTTTAAAAAAAACGCGACATTTGTATGGGACGCG‑TTGCGTAAGTGATGATGCAAAAGGGGTTCTTTTCAGGGGCCGTCCCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAacc < 2:1145685/237‑1 (MQ=255) agTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CATGCCCATACCTTTTGGCCAGCCGTGGGGGGAacc > 2:1185605/1‑238 (MQ=255) agTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCCCCCTTGGCTGCCAC‑CAGCGCAATACCTTTTGCCCAGTCGTGTGGAGAacc > 2:486526/1‑238 (MQ=255) agTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTCCGGCTTTGACAG‑‑GCGGTCAACGCCTTCGTAATCCGGAATATCACCGCCATTGGCTGCGCC‑CATCCCAAAACCTTTTGCACAGCAGTGTGGAAAaca > 1:138554/1‑237 (MQ=255) tCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCCC‑CACCGCAATACCTTTTGCGCAGTCGTGTGGAGAaccac > 2:600087/1‑238 (MQ=255) tCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGAGGTTTTG‑CAGA‑GCGGTCAACGCCCTCGTAATCGCGAATATCGCCGCCCTTTCGTGCCCC‑CAGCGCAATACCTTTTGCGCAAGCGCGTGGAGGaccac > 2:932679/1‑238 (MQ=255) aaaaaagcggggggtgaaCATTTTTACCAACAATCGCCTTTTGGATAGGCCGCG‑CTTCGGCAGTGATGATGCAAAAGGGGGTCATTCCAGGGCCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAg < 2:455745/222‑1 (MQ=255) aGAAAGCAGCGAAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGACGTGTGGGGAACCACCGCC‑CAAgg > 2:906180/1‑239 (MQ=255) agcagcGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGGGAACCACCGCC‑TAAGgaga > 2:395147/1‑238 (MQ=255) cGGAGTGACATGTTTATCAACAATCGACATTTTGATATGACGTG‑CTTCGTCAGTGCTGATGCAGAAACGGGTCATTTCAGAGGCCGTACCA‑GCCGTGGTATTGCTGGCGATAATCGTCAGATTCGGTTTTG‑TCGT‑GCGGTGAACGGCTTCCAAAGCCGGGATGTCGCCGCCATCTGCAGCCCCAAAGTGC‑CTACCCTTTGCGCCTTCGTGTGgagacataagcctaaaagggaaacgga > 1:875051/1‑216 (MQ=255) ggAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCCCCATTGGCTGCCAC‑CAGCCCAATACCTTTTTCGCAGTCGTGTGGGAGACCACCGCC‑TAAGGAGATCACGCt > 2:582421/1‑239 (MQ=255) aGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCCATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCtt > 2:1106262/1‑237 (MQ=255) gTGACTTTTTTAACAACAACCGCTGTTGTGATAGGCCGCG‑CTTCGTCAGTGAGGGTGGAAAAGCGGTTTTTTACAGGGGCCTACCCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTAt < 2:516877/238‑1 (MQ=255) tttacaaccatccgcttttttGATATG‑CGCGCCTTGGTCAGTATGGTTGCAGACCCGGGTTATTCAAGGGCCCTTCCCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑attat < 2:1242715/222‑1 (MQ=255) aTCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGG‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAACGCAATACCTTTTGCGCAGTCGTGTGGCAAACCACCACC‑TACggcaatacggcattccgaatttttt > 2:469717/1‑215 (MQ=255) tccgctttttGATAGGCGGCG‑CTTCGTCAGGTAGTATGAAGAAACGGTTAATTTCGGGGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGt < 2:1277402/233‑1 (MQ=255) tCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCCC‑CAGCGCAATACCTTTTGCGCAGTCGTGGGGAGAACCACCGCC‑TAAGGAGATCACGCTATCCCA‑ATTATTCTCTTTAAGt > 2:1170260/1‑239 (MQ=255) tGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCCCGCTATCGCA‑CTTATTCTCTTTAAGTAATTTCAAACCTgc > 2:1275056/1‑239 (MQ=255) cgcg‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTgcggc > 2:1200775/1‑239 (MQ=255) cTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATTCCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTGTTGCGCAGTCGTGTGGAGAACCCCCGCC‑AAAGGGGATCACACCATCGCACATTTTCCTTTTTAAG‑AATTTAAAACCTagggcggag > 1:1080883/1‑236 (MQ=255) cTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTTTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCC‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGGGACg > 2:18479/1‑239 (MQ=255) cGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCCCCGCC‑TAAGGAGATCACCACATCCCA‑ATTATTCTCTTTAAGGAATTTCAAACCTGCGGGGACGttt > 2:1114431/1‑239 (MQ=255) gccatgtagggtgaaaaaCGGGGCATTTGAGGGGCCCTGCCCGGCCGTGGTATGGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGtttt < 2:122763/224‑1 (MQ=255) atgatgCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCgtggt > 2:702732/1‑239 (MQ=255) atgCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCCCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTggg > 2:784136/1‑239 (MQ=255) aaggggtgtttttaggtggcctgaCCC‑GCGGGGGTTATGGTGGCGATCACCGGCAGCTGCGTTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAgg < 2:957044/216‑1 (MQ=255) aCGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGtt > 2:858077/1‑239 (MQ=255) tatttcgagggccgcgaCCC‑GCGTTGGTATGGATGGCGATCATCGGACGCTGCGGTTTTG‑CAGG‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTg < 2:573819/223‑1 (MQ=255) cttttcgtgggggcttcccc‑GCCGTGGTTTGTATGGCGATCGCCGGGATCTGCGGTTTTG‑‑AGAGGCGGTAAACGCCGTCTTAATCCGGAATTCCGCCGCCATTGGGTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTg < 1:18479/222‑1 (MQ=255) ctttccgggggcgttACCC‑GCCGTGGTATTGATGGCGATCATCGGAAGCTGCGGTTTTG‑CAGG‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTg < 2:1053227/224‑1 (MQ=255) tCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAg > 2:809598/1‑153 (MQ=255) acccggtggtggtttGATGGGGATCATCGGCAGGTGCGTTTTGG‑CAGA‑GCGGTCAACGCTTTCGTAACCGCGAATAGCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAATCCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGc < 2:533244/226‑1 (MQ=255) tGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAata > 2:1171860/1‑239 (MQ=255) ggCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGttct > 2:959910/1‑238 (MQ=255) cTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTTCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTTAAAAATTTTCGTTCTTCCAGTGCTTTTTGc > 2:1298481/1‑238 (MQ=255) gCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGGGGAGAACCCCCGCC‑TAAGGAGATCACGCTATCGCA‑ATGATTCTCTTTAAGTAATTTCAAACCTGCGGCCACGTTTTCCGTGTGGGGGTTAGGTTGGGGGCCACCATAAAAAACCACAAAAAAATTTGGTTTTTcccagcgttttttaact > 1:721156/1‑224 (MQ=255) gggTTTTG‑CAGG‑GGGGGCAACGTCTTGATAACGGGAAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGAGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGcaca < 2:12342/237‑1 (MQ=255) cGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATAcc > 2:1047308/1‑239 (MQ=255) cGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGCAATTTCAAACCTGCGGCGACGTTTTCCGTGGCGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCCCATAGCCCGCCATAcc > 2:328529/1‑239 (MQ=255) cTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCCGTGCCTTTTGCACATCGCCCGCCATACCCAATTTct > 2:90947/1‑236 (MQ=255) cTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGGGCCATCCTAAATAACGCTAAAAATATTGCTTTCTTCCAGGGCTTTTTGCACATCTCCCGCCATAACTAATTTCGtt > 2:851607/1‑239 (MQ=255) ctggaacccacaatttcccctataTTGGCTCCAAC‑CAGCGCAACTCCTTTGGCGCAGTGGTGTGGAGACCCCCCGCC‑TAAGGAGATCACGAGTTCGAA‑ATTATTCTCTTTAAGTAATTTCAAACCGGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGTTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTaa < 2:34479/216‑1 (MQ=255) aTCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACatat > 2:241500/1‑239 (MQ=255) tCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCCTTTGCGCAGTCGTGTGAAGAACCACCGCC‑TAAGGAGATCACGCTATTGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTTCGGCGACGTTTTCCTTGGTGGGTCTCGGTAGGGTGCCATCATAAATAACGTTAAAAAAATTGCGTTCTTCTAGTGCTTTTTGTACTTAGCCGCACATACCTAATTTTGTaaaattata > 1:1263247/1‑233 (MQ=255) gcccATTGGTTGCCCC‑CAGCGCAATCCCTTTTGCGCATTCGTGTGGAAACCCCCCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCGGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAAtt < 2:721156/237‑1 (MQ=255) gtGGCGGCCAC‑CCGCGCAATCCCTTGTGCGCTGTTGTGTGGAAACCCCCCGCC‑AAAGGAGATCACGTTATCGAA‑ATTATTCCTTTTAAGTATTGTCAACCCGGCGGCGACGTTTTCCGGGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGt < 2:672703/238‑1 (MQ=255) cac‑caGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTaa > 2:592419/1‑239 (MQ=255) aGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAACATATTGCGTTCTTCCAGGGCTTTTTTCACATCGCCCGCCAAAACTAATTTCGTTAACCAATGTTCAGTGACAATAAAGGTACGGGAAAAAcc > 1:535082/1‑239 (MQ=255) aTACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAAt > 2:652667/1‑238 (MQ=255) cccTTTTGCGAAGTCGTGTGGAAACCCCCCCCC‑TAAGGAGTTCACCCTATCGCA‑ATTATTCTTTTTAAGTAATTCCAAACCTGCGGCGACGTTTCCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATTTTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTg < 2:197255/238‑1 (MQ=255) cttttttccgtgTCGTGGGGGAAACCCCCCCCTTAAGGGTATCCCGTTATCGCA‑TTTATTCTCTTTAAGTAATGACAACCCGGGGGCACCGTTTTCGGTGGGGGGTTTAGGTTGGGGTCATTCATAAATAACGATAAAATTTTTGCTTTCTTCCAGTGCTTTTTGCCCATCTCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTg < 1:959910/228‑1 (MQ=255) tttGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAACAATATTGCGTTCTTCCAGTGCTTTTTGCCCATCGCCCGCCATACCTAATTTCGTTACCATATTGTCAGGGACAATTAAGGTACGGGTaaaacaaaaaaatcccaa > 1:197255/1‑224 (MQ=255) tttGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCcat > 2:206684/1‑239 (MQ=255) gcAGTCGGTGGGAGAACCCCCTCC‑AAAGGAGATCACGCTATCGCA‑TTTATTCTCTTTAAGTAATTTCAACCCTGCGGCGACGTTTTCCGTGGTGGGTTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCata < 2:753528/238‑1 (MQ=255) aGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAg > 2:409985/1‑195 (MQ=255) ggcggggggAAAACCACCGCT‑GAGGGAGATCCCGTTATCGCA‑ATTATTCTCTTAAAGTAATTCCAACCCGGCGGCGACGTTTCCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCa < 2:249176/232‑1 (MQ=255) ggcGTGGGGAAACCCCCCGAC‑TAAGGAGATCAGGTTTTCGAA‑ATTTTTCTCTTAAAGTAATTTAAAACCTGGGGCGACGTTTTCCGTGGTGGGGTTAGTTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCa < 2:138554/237‑1 (MQ=255) gTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTCACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCAAAATTTGCcaatacattaa > 1:254644/1‑230 (MQ=255) gTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGAGCTTTTTGCACATCGCCCTCCAACCCTAATTTCGTTAACATATTTTCATCGGCAATTAAGGTACGGTGAAAACCATAAACTGAcataacataaa > 1:677542/1‑231 (MQ=255) tCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATTTTGTCAGTGACAATTCAGGAACCGGTAAACCCAAAATATCCCATCATAATCAt > 1:310368/1‑239 (MQ=255) tCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATATGCCATCATATTCAt > 2:40324/1‑239 (MQ=255) | AGTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCG‑CTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCC‑GCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGA‑GCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCAC‑CAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCAT > NC_000913/3757298‑3757760 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |