Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,498,624 Δ1 bp 6.6% intergenic (+113/‑27) opgD → / → ydcH OPG biosynthetic periplasmic protein/DUF465 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,498,6230C. 6.6% 210.3 / 7.9 61intergenic (+112/‑28)opgD/ydcHOPG biosynthetic periplasmic protein/DUF465 family protein
Reads supporting (aligned to +/- strand):  ref base C (30/27);  new base . (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

TATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAAC  >  NC_000913/1498344‑1498911
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                
tATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGc                                                                                                                                                                                                                                                                                         <  2:533925/289‑1 (MQ=255)
   cGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCAc                                                                                                                                                                                                                                                                                      <  1:234155/289‑1 (MQ=255)
        tCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACa                                                                                                                                                                                                                                                                                 <  1:192334/289‑1 (MQ=255)
         cAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAg                                                                                                                                                                                                                                                                                >  1:125883/1‑289 (MQ=255)
          aGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAgg                                                                                                                                                                                                                                                                               <  2:140801/289‑1 (MQ=255)
                    gTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCTGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTAt                                                                                                                                                                                                                                                                     >  1:349384/1‑289 (MQ=255)
                    gTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTAt                                                                                                                                                                                                                                                                     >  2:312921/1‑289 (MQ=255)
                               cGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAgg                                                                                                                                                                                                                                                                               <  1:131356/268‑1 (MQ=255)
                               cGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAgg                                                                                                                                                                                                                                                                               >  2:131356/1‑268 (MQ=255)
                                   cTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCg                                                                                                                                                                                                                                                      <  2:60558/289‑1 (MQ=255)
                                                gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATc                                                                                                                                                                                                                                          >  2:249/1‑288 (MQ=255)
                                                     tatGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCg                                                                                                                                                                                                                                                      >  1:114145/1‑271 (MQ=255)
                                                     tatGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCg                                                                                                                                                                                                                                                      <  2:114145/271‑1 (MQ=255)
                                                           gATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGaaaaa                                                                                                                                                                                                                              >  2:486647/1‑289 (MQ=255)
                                                           gATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGaaaa                                                                                                                                                                                                                               >  1:418161/1‑288 (MQ=255)
                                                                   tACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATc                                                                                                                                                                                                                      <  2:125883/289‑1 (MQ=255)
                                                                    aCGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATcc                                                                                                                                                                                                                     <  1:486647/289‑1 (MQ=255)
                                                                     cGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCTGATACACCTTGTCAGCCACTAACAGGGAATGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    <  2:227026/289‑1 (MQ=255)
                                                                     cGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    <  1:249/289‑1 (MQ=255)
                                                                     cGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    <  2:269975/289‑1 (MQ=255)
                                                                     cGTTGTCAGAGGGACGCTAGTAGTGAAACATGGCTGTAGCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCGTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGGATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    <  2:16427/289‑1 (MQ=255)
                                                                      gTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTc                                                                                                                                                                                                                   >  1:265188/1‑289 (MQ=255)
                                                                       ttGTCGGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCTCCGGGTAAACGTCAGTATGTTGACGACCGCGTGATGCGTTAATCGTTTGTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    <  2:117630/287‑1 (MQ=255)
                                                                        tGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGGGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    >  2:34999/1‑285 (MQ=255)
                                                                        tGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGGGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    <  1:34999/285‑1 (MQ=255)
                                                                        tGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    <  1:229519/285‑1 (MQ=255)
                                                                        tGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    >  2:229519/1‑285 (MQ=255)
                                                                         gTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGATTACCGAGTTTTAATATCCCGTCTGAAAAACGAAAATCCttcct                                                                                                                                                                                                                >  2:520911/1‑285 (MQ=255)
                                                                                           gTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATAcc                                                                                                                                                                                                                                                       >  2:301716/1‑232 (MQ=255)
                                                                                           gTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATAcc                                                                                                                                                                                                                                                       <  1:301716/232‑1 (MQ=255)
                                                                                              aaaCATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATaaa                                                                                                                                                                                            <  1:520911/288‑1 (MQ=255)
                                                                                                aCATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAacac                                                                                                                                                                                         >  1:421954/1‑289 (MQ=255)
                                                                                                                    cccGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAAt                                                                                                                                                                     >  2:28706/1‑289 (MQ=255)
                                                                                                                        ccAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCCCCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGATTAATCTGATGGTGTATATTTCATATACACCTTGTCAGCCACTAACAGGTAGTGCGTATGTTTCCATAATACCGAGATTTAATATCCCGTCTGAAAAACGACAATCCTCGCTTTATGTCCTTCTTCGATAAACACAATAAACTTTATCATGCAATTTcc                                                                                                                                                                 >  1:455556/1‑289 (MQ=255)
                                                                                                                         cAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCAGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCa                                                                                                                                                                >  2:273523/1‑289 (MQ=255)
                                                                                                                               cGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAaagg                                                                                                                                                          <  1:242637/289‑1 (MQ=255)
                                                                                                                                                     gcactgggTGATGGGTTAAACGTTTTTTTTCAGGAACCTTATTCAGGAGGGGCCGTCTGGTTAGACACGGTCCCGATCGCATTTTTCCCTAAGTTAAATGAGTAATGTGATGGTTTGTATTTCATATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTa                                                                                                                                    <  2:455556/282‑1 (MQ=255)
                                                                                                                                                         cgcgTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCgag                                                                                                                                                                                                                                                    <  1:514533/173‑1 (MQ=255)
                                                                                                                                                         cgcgTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCgag                                                                                                                                                                                                                                                    >  2:514533/1‑173 (MQ=255)
                                                                                                                                                         cgcgTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAAt                                                                                                                                >  1:376885/1‑289 (MQ=255)
                                                                                                                                                                            tttttCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATCCGGAAGTGGTCCGCATGa                                                                                                             >  1:203062/1‑289 (MQ=255)
                                                                                                                                                                             ttttCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGaa                                                                                                            >  2:174654/1‑289 (MQ=255)
                                                                                                                                                                                            tcttcGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACa                                                                                             >  1:280308/1‑289 (MQ=255)
                                                                                                                                                                                                      gTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAagat                                                                                   >  1:486527/1‑289 (MQ=255)
                                                                                                                                                                                                        gCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAgg                                                                                                                                                                                                                                                                               >  1:331294/1‑99 (MQ=255)
                                                                                                                                                                                                        gCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAgg                                                                                                                                                                                                                                                                               <  2:331294/99‑1 (MQ=255)
                                                                                                                                                                                                          cGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAagatgag                                                                                <  1:28706/288‑1 (MQ=255)
                                                                                                                                                                                                                      cacGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATcc                                                                   <  2:486527/289‑1 (MQ=255)
                                                                                                                                                                                                                          aTCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTgca                                                               >  2:414526/1‑289 (MQ=255)
                                                                                                                                                                                                                          aTCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCAGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTa                                                                                               >  1:518477/1‑257 (MQ=255)
                                                                                                                                                                                                                          aTCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCAGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTa                                                                                               <  2:518477/257‑1 (MQ=255)
                                                                                                                                                                                                                                cTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGt                                                                                                                                            >  1:168110/1‑206 (MQ=255)
                                                                                                                                                                                                                                cTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGt                                                                                                                                            <  2:168110/206‑1 (MQ=255)
                                                                                                                                                                                                                                          tCCCTAAGTTAAATTAGTAATCTGATGGTGTGTATTTCCAATACACCGTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCaaa                                               <  1:483666/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tgtATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTg                                                                                                                                                                                                                                                                          >  2:403830/1‑40 (MQ=255)
                                                                                                                                                                                                                                                                        tgtATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTg                                                                                                                                                                                                                                                                          <  1:403830/40‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tgtATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCgccagcca                 >  1:188671/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        tgtATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCgccagcca                 >  2:217218/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          tATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    <  2:429943/92‑1 (MQ=255)
                                                                                                                                                                                                                                                                          tATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCt                                                                                                                                                                                                                    >  1:429943/1‑92 (MQ=255)
                                                                                                                                                                                                                                                                             ttCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAggcg             >  1:169/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                       ccTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTaac  <  2:421954/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                
TATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAAC  >  NC_000913/1498344‑1498911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: