Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,157,049	A→G	5.4%	E67E (GAA→GAG)	argH →	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,157,049	0	A	G	5.4%	251.8 / 3.2	75	E67E (GAA→GAG)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base A (35/35);  new base G (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

GGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTACACTCACCTGCAACGCGCC  >  NC_000913/4156767‑4157328                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                        gggTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGtt                                                                                                                                                                                                                                                                                   <  2:84676/289‑1 (MQ=255)      cgcgGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTcgcg                                                                                                                                                                                                                                                                               >  1:210326/1‑288 (MQ=255)      cgcgGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCgcgc                                                                                                                                                                                                                                                                              <  1:464926/289‑1 (MQ=255)       gcgGAGTGTAGATTAAATTTGGTGGGCCGGGGGGCCAGCGTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTGCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAGGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCgcgc                                                                                                                                                                                                                                                                              <  2:335700/288‑1 (MQ=255)        cgGATTTTAGCTTAAGTTTTGTTGGCCGGAGCCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGAGCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCTCTGAACGTGTTGCTGGAAGATGTTCgcgc                                                                                                                                                                                                                                                                              <  2:390132/287‑1 (MQ=255)                            gttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGTCACTGGAAGAGGCGCTGAACGTGTTGCTGGACGATGTTCGCGGCAGGCCACAACAAAACCGTGaa                                                                                                                                                                                                                                                        >  2:362707/1‑289 (MQ=255)                            gttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGaa                                                                                                                                                                                                                                                        >  1:539194/1‑289 (MQ=255)                                    gAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGaa                                                                                                                                                                                                                                                                                         <  1:384346/248‑1 (MQ=255)                                    gAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGaa                                                                                                                                                                                                                                                                                         >  2:384346/1‑248 (MQ=255)                                     aGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGc                                                                                                                                                                                                                                                <  2:539194/288‑1 (MQ=255)                                         gcAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCGCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAg                                                                                                                                                                                                                                           <  2:380016/289‑1 (MQ=255)                                                       ttGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTgg                                                                                                                                                                                                                             >  2:551179/1‑289 (MQ=255)                                                                           aCAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCGCTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGa                                                                                                                                                                                                         <  1:547291/289‑1 (MQ=255)                                                                               agTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACaaa                                                                                                                                                                                                     >  1:230742/1‑289 (MQ=255)                                                                                gTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACa                                                                                                                                                                                                       <  2:379615/286‑1 (MQ=255)                                                                                 ttATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGt                                                                                                                                                                                                   >  1:475919/1‑289 (MQ=255)                                                                                 ttATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGt                                                                                                                                                                                                   >  2:210211/1‑289 (MQ=255)                                                                                         cTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTTGTCCAAAGCCCTGGTCACGGTTGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGACCGTGTTGCTGGAGGATGTTCGCGCCAGTCTACAACAAATCCTTTAAAGCGCCGCCGAAGATATCCATACCTGGGTGGAAGGAAAACTGATCGGCTAAGGGGGACAGt                                                                                                                                                                                           >  2:535164/1‑289 (MQ=255)                                                                                         cTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGAGGAAGGCAAACTGATCGACAAAGTGGGCCAGt                                                                                                                                                                                           >  1:416159/1‑289 (MQ=255)                                                                                          tttGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGtt                                                                                                                                                                                          <  2:330346/289‑1 (MQ=255)                                                                                           ttGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTa                                                                                                                                                                                         >  1:158964/1‑289 (MQ=255)                                                                                                    gcgTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCaaaaa                                                                                                                                                                                 >  1:533360/1‑288 (MQ=255)                                                                                                        tttACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTg                                                                                                                                                                             <  1:210211/288‑1 (MQ=255)                                                                                                            cccAGGCAGAAGATCAACTGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCGGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATAc                                                                                                                                                                        <  1:378040/289‑1 (MQ=255)                                                                                                             ccAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATAcc                                                                                                                                                                       <  1:171161/289‑1 (MQ=255)                                                                                                                ggCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCgg                                                                                                                                                                     >  1:413670/1‑288 (MQ=255)                                                                                                                ggCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCAAGTTAGGCAAAAAGCTGCATACCggg                                                                                                                                                                    >  2:331882/1‑289 (MQ=255)                                                                                                                   cgcagATCAACGGGTCAAAAAATTCAACGATTCCGTGCGCTTTGATTACCGGTGGGCGGAGCATGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGt                                                                                                                                                                 <  2:210326/288‑1 (MQ=255)                                                                                                                   agcagATAAAAGGTTCAAAAAATTCAACGACTCACTGGGGTTTGATTACCGTCTGGAGGGGCAGGATATTGTTGGCTATGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGt                                                                                                                                                                 <  2:26224/289‑1 (MQ=255)                                                                                                                     caagtCAACGGGTCAAACAATTCAGAGACTCCCGGGGGTTTGATTACCGTCTGGCGGAGCGAGATATTGTTGGCTCTGTGGCCGGGGCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGGGGCGCTGAACGGGTTGCTGGAAGATGTTGGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAg                                                                                                                                                               <  2:523297/285‑1 (MQ=255)                                                                                                                                  cAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAg                                                                                                                                                  >  2:98621/1‑289 (MQ=255)                                                                                                                                          tCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGAc                                                                                                                                          <  1:331882/289‑1 (MQ=255)                                                                                                                                              cGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGa                                                                                                                                       >  1:103246/1‑288 (MQ=255)                                                                                                                                               gcatCACGGCGCTTTGATTACCGTGGGGCGGAGCAGTATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGAcc                                                                                                                                     <  2:395231/286‑1 (MQ=255)                                                                                                                                                        gtcttgtgttACCGTCTGGCGGGGCAGGATATTGTTGGCGCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACtg                                                                                                                             <  2:413670/280‑1 (MQ=255)                                                                                                                                                        cgcTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGAcc                                                                                                                                     <  1:229775/280‑1 (MQ=255)                                                                                                                                                        cgcTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGAcc                                                                                                                                     >  2:229775/1‑280 (MQ=255)                                                                                                                                                          cTTTGATTACCGTCTGGCGGAGCAGGATGTTGGTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAGGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTgtgg                                                                                                                          <  1:535164/289‑1 (MQ=255)                                                                                                                                                                         ggCGGAGCAGGCTATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGACGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTTGGTGGACGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGGCGTAATGATCAGGTAGCGACTGACCTGACACTGTGGTGCATAGTTACCGtt                                                                                                           >  2:8176/1‑289 (MQ=255)                                                                                                                                                                             gaggaggagATTGTTGGATCGGTGGGCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGGGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGc                                                                                                        <  2:116614/288‑1 (MQ=255)                                                                                                                                                                             gAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGc                                                                                                        <  2:103246/288‑1 (MQ=255)                                                                                                                                                                             gAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGc                                                                                                        <  2:533360/288‑1 (MQ=255)                                                                                                                                                                             gAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGc                                                                                                        <  1:551179/288‑1 (MQ=255)                                                                                                                                                                                         gttgGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGACCGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACgg                                                                                           <  1:362707/289‑1 (MQ=255)                                                                                                                                                                                               tctGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAACGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCCACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGAGCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAAcc                                                                                     >  2:398596/1‑289 (MQ=255)                                                                                                                                                                                               tctGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAAc                                                                                      >  2:80075/1‑288 (MQ=255)                                                                                                                                                                                                 tgtgGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACt                                                                                                <  2:110212/276‑1 (MQ=255)                                                                                                                                                                                                 tgtgGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACt                                                                                                >  1:110212/1‑276 (MQ=255)                                                                                                                                                                                                 tgtgGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCg                                                                                    >  2:218967/1‑288 (MQ=255)                                                                                                                                                                                                  gtgGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGc                                                                                  >  1:89089/1‑289 (MQ=255)                                                                                                                                                                                                           tCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCaa                                                                          >  1:292885/1‑288 (MQ=255)                                                                                                                                                                                                             cAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCg                                                                       >  2:178966/1‑289 (MQ=255)                                                                                                                                                                                                               aaGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGgc                                                                     >  1:101453/1‑289 (MQ=255)                                                                                                                                                                                                                     tGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCgcgc                                                                                                                                                                                                                                                                              >  1:224462/1‑82 (MQ=255)                                                                                                                                                                                                                     tGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCgcgc                                                                                                                                                                                                                                                                              <  2:224462/82‑1 (MQ=255)                                                                                                                                                                                                                     tGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCtggt                                                               >  1:346067/1‑289 (MQ=255)                                                                                                                                                                                                                     tGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCtggt                                                               >  2:424802/1‑289 (MQ=255)                                                                                                                                                                                                                                      ttAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACa                                              >  2:319136/1‑289 (MQ=255)                                                                                                                                                                                                                                        aaCCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAAt                                            <  1:509354/289‑1 (MQ=255)                                                                                                                                                                                                                                         acggcggaagaGCAGGCGCAACTGGAAAGGGCGTGGAACGTGTTGCTGGAAGCGGTGCGCGCAAGGCCAAACAAAACCCTTGAAAGCGACGCCGAAGATATCCAAAGCGGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCAAAAAACAATc                                           <  2:101453/283‑1 (MQ=255)                                                                                                                                                                                                                                              agaagaGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCTATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGACACTGTGGTGCAAAGATACCGTTAGCGAGTTAGTGAAGTCTACACGGCAGCTGCAATCGGCGCGGTGGGCAACCGCAAAACACACACAGGAc                                      >  2:407598/1‑289 (MQ=255)                                                                                                                                                                                                                                                aagaGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGAcgc                                    >  1:460934/1‑289 (MQ=255)                                                                                                                                                                                                                                                 agagCATGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGGTCGCGACAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGAcgcg                                   <  2:423963/289‑1 (MQ=255)                                                                                                                                                                                                                                                    gCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGt                                 <  1:178966/288‑1 (MQ=255)                                                                                                                                                                                                                                                     cAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAGGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTaa                               >  2:192307/1‑289 (MQ=255)                                                                                                                                                                                                                                                     cAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGa                                       <  1:504438/281‑1 (MQ=255)                                                                                                                                                                                                                                                     cAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGa                                       >  2:504438/1‑281 (MQ=255)                                                                                                                                                                                                                                                     cAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGGGGAAACCGCACAAAACCATCAGGACGCGGCaa                               >  2:297654/1‑289 (MQ=255)                                                                                                                                                                                                                                                        gcgcATCTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATg                             >  1:92046/1‑288 (MQ=255)                                                                                                                                                                                                                                                         cgcAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATg                             <  1:80075/287‑1 (MQ=255)                                                                                                                                                                                                                                                                  aaGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATTAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTaca                   <  2:158964/288‑1 (MQ=255)                                                                                                                                                                                                                                                                  aaGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTaca                   <  1:218967/288‑1 (MQ=255)                                                                                                                                                                                                                                                                  aaGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTaca                   <  1:297654/288‑1 (MQ=255)                                                                                                                                                                                                                                                                   agagGCGCTGAACGTGTTGCTGGAGGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTACACt                 <  1:192307/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gtTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTACACTCACCTGCAACgcgc   >  1:183256/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                  gtTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTACACTCACCTGCAACgcgc   >  1:555892/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                   ttGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTACACTCACCTGCAACGCGcc  >  2:31469/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                        GGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGTTACACTCACCTGCAACGCGCC  >  NC_000913/4156767‑4157328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑