Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,326,049	G→A	7.3%	intergenic (‑307/+351)	yjdN ← / ← yjdM	metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,326,049	0	G	A	7.3%	137.5 / 3.4	55	intergenic (‑307/+351)	yjdN/yjdM	metalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base A (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

AGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGA‑TGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAT  >  NC_000913/4325765‑4326300                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                               aGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTg                                                                                                                                                                                                                                                             >  1:356874/1‑288 (MQ=255)   ccAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCCGGCCGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCGGGTGCGGGCATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCCTCACGCAGTCGGCCCCGTTGCCg                                                                                                                                                                                                                                                          >  2:452714/1‑289 (MQ=255)                         ttagagaggtGAGAGGCGGCCGGATAAGAGCGGCGGCCTTCGACTCGGGCGGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                                                                                                    <  2:252482/280‑1 (MQ=14)                         ttATACAGAGGGGAGGCGGCCTGATAAGACGCGCGAGCGTCGAATCAGGCAGTCGGCACGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                                                                                                    <  2:165792/289‑1 (MQ=255)                                      tgtaggcCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGcc                                                                                                                                                                                                                                         <  2:556363/267‑1 (MQ=2)                                      tgtaggcCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGcc                                                                                                                                                                                                                                         >  1:556363/5‑271 (MQ=2)                                      agGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCATTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATg                                                                                                                                                                                                                      >  1:460470/1‑289 (MQ=39)                                         cggcCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTg                                                                                                                                                                                                                   >  1:102169/1‑289 (MQ=38)                                                   aaacgggcaggcgtcgcgtcAGCAAGTCGGAACGGTTGCCGGTGGCGGGCTAAACGCCTGATCCGGCGAGCGGAGGGTGCGGGAATTTGTAGGCCTGTTAAGAAGGGCAAGCGTCGCATCAGGCAGTCGGAACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTg                                                                                                                                                                                                         <  2:252459/279‑1 (MQ=11)                                                      gagcgaggggtgcatgtaGGCAGTGGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTa                                                                                                                                                                                                       <  2:268815/271‑1 (MQ=255)                                                       gcgcgggggggggcagaagCAGTGGGAACTGTTGCGGGATGCGGCGTAAAGCACTTATCGGGCCTAGGGAGAGTGCGGGAATTGGGAGGCCGGATACGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAg                                                                                                                                                                                                      <  2:302221/270‑1 (MQ=11)                                                         gcgAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGc                                                                                                                                                                                                    <  1:226201/288‑1 (MQ=25)                                                          cgAGCGTCGCATCAGGCCGTCGGCCCTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGACTGATAAGACCCGCAAGCGTCGCACCAGGCACTCGGCCCCGTTTTCGGATGCGGCGGATGCGCCCTATCCGGCCTGCGGAGGGTCCGGGAACCTGTTGTCCCCGTCAGCCGCGCAAGCGTTGCATCAGTCACTCGGGCCCTTTGCCCGCTGCCGCGTAAACGCCTTCTCCTGccccagggatcataggtagtcttgcatgcc                                                                                                                                                                                                   >  2:143994/1‑261 (MQ=2)                                                                tCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACCCCTTATCCGGCCT‑CCGGAGGGTGCGGGAAGTTGTAGGACTGATAAg                                                                                                                                                                                            >  2:317390/1‑289 (MQ=12)                                                                           aGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑g                                                                                                                                                                                <  2:356874/289‑1 (MQ=37)                                                                             tCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGGAGGGTGAGGGAATTTGTAGGAATGATAAGAAGAGAAAGAGTAGAATAAGGAAGTAGGAAAAATTGAAGGATGAGGAGTAAAAGAATTATAAGGAATAAGGAGGGTGAGGGAATTTGTAGGAATGATAAGAAGAGAAAGAGTAGAATAAGGAAGTAGGAAAAGTTGAGGGATGAGGAGTAAAAGAATTATAAGGAAT‑AAGGATGGTGAGGGAATTTGTAGGAATGATAAGaagagaaagagt                                                                                                                                                                                <  1:50371/288‑12 (MQ=9)                                                                               ggCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATCGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCg                                                                                                                                                                             <  1:87522/288‑1 (MQ=37)                                                                                           ggATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAACTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCTGATGCGGGGTAAACGCCCTCTACCGCCTCACGG‑TGGTTGGGGGGTGTGGAGGCTTGATTAGAAgcgccatcgcggcctgcggacgttg                                                                                                                                                                 >  2:520633/1‑268 (MQ=11)                                                                                               ggggcgTAAAGGGCGTAGCCGGCGTAGGGAGGGGGCGGGAAGTGGTAGGCCTGATAAGACGGCGAAGCGCAGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCa                                                                                                                                                             <  2:357240/286‑1 (MQ=11)                                                                                               gcggcgTAAGCGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCa                                                                                                                                                             <  1:102346/288‑1 (MQ=37)                                                                                                cggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCATTCTCCGGCCT‑CCCGATGGTGCGGGCTTTTGTATGTCTGATAAACAGCCCAACC‑CACGCATCAGGcgcgtggc                                                                                                                                                              >  2:371099/1‑279 (MQ=11)                                                                                                             ttATCCGGCCTACGGAGGGGGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATgcg                                                                                                                                              <  1:520633/289‑1 (MQ=38)                                                                                                                    gCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGATCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCTGGCAAGTTGCGCTGTTGCCGGACGCGGCGtgat                                                                                                                                       >  2:195312/1‑286 (MQ=11)                                                                                                                     ccTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATg                                                                                                                                                                                                                                                      <  2:36/179‑1 (MQ=38)                                                                                                                               gTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAAc                                                                                                                                                                                                                                            >  1:36/1‑179 (MQ=35)                                                                                                                               gTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                                                                                                                                     >  2:125354/1‑186 (MQ=35)                                                                                                                                   gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGTCGTCAATGCCTTATCCGGCCT‑ACGGCCGGTGCGGGCATTCGTCGGCGTGAGAACACGCGCACCCGGCCGCCCCAGCCA‑CTGGCCCTATTGCCGGATGTGGCGtcacagcatttgcaggcc                                                                                                                         >  2:422841/1‑271 (MQ=11)                                                                                                                                       aTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCATAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                                                                                                     <  1:422687/288‑1 (MQ=38)                                                                                                                                        tttGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGACTTTGTTGGCCTGATAAGACGCCCAAGC‑CTCGCTCCCGGCAGTTGGCACCCTTGTCGGGTTCGGCGTCAGCCCCTTCACCGGGCCACGGa                                                                                                                   >  2:258018/1‑289 (MQ=11)                                                                                                                                             aGGCCTGATAAGACGCGCCGGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                                                                                                              <  1:194302/289‑1 (MQ=38)                                                                                                                                                           ccgcAAGCGTCGCATCAGGGAGGCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGGAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAg                                                                                                 <  2:194347/287‑1 (MQ=255)                                                                                                                                                                            ggCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTCGGTCTGATAAGACGCCCACGC‑GTCGCATCCGGCAGTTGGCACTGTTGCCGGATGCGGCGTGAATGTCTTATCCCGCCTACGCACGGTTCGGGGATTTGTAGGCCCGATAAGACccgcca                                                                               >  1:444771/1‑287 (MQ=11)                                                                                                                                                                                gtcgggacggtggCCGGATGGGGCGTGAAAGCGTTATCCGGGCTACGGAGGGTGCGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGCTGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCg                                                                            <  2:102169/276‑1 (MQ=11)                                                                                                                                                                                 tCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                               >  1:241548/1‑236 (MQ=38)                                                                                                                                                                                 tCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                               <  2:241548/236‑1 (MQ=38)                                                                                                                                                                                      aCCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCCCATCACGCAGCTGGCACTGTTGCCGGATGCGGCGTTACCGCCTTGACCTGCCTACGGAGGGTGCTGGAAGTTGTAGGCCTGATatgcaccccactcgtcgcct                                                                      >  2:470965/1‑269 (MQ=11)                                                                                                                                                                                           tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTATGCCTGATAAGACGGGCCAGCCTCGCCTCACGc                                                                 >  2:81255/1‑288 (MQ=255)                                                                                                                                                                                                      gcgTAGACGCATTATCCGGCCTACGGAGGGGGCGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTGGGATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCg                                                      <  2:119651/288‑1 (MQ=255)                                                                                                                                                                                                                             cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAg                                                                                                                                                                       <  1:51156/152‑1 (MQ=31)                                                                                                                                                                                                                             cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCg                                                                            >  2:345287/1‑243 (MQ=37)                                                                                                                                                                                                                             cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCg                                                                            <  1:345287/243‑1 (MQ=37)                                                                                                                                                                                                                                  ggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATc                                                                                                                                                                         <  1:277664/145‑1 (MQ=32)                                                                                                                                                                                                                                  ggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGCGCGGGAATTTGTACGCCCTATCATACCCCCCAGCGACGCCTCCGGGCTGCGGCACCTTTGCCGGACACCGGCGTCAACGCtttt                           >  2:50613/1‑286 (MQ=11)                                                                                                                                                                                                                                    tGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCATGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTCCGGAGGGTGGCGGAATTTGTAGGCTTGAAACAACGCGCAAGAGCCGCCTCAGGGCGTCGTCACCCTTACCGGA‑CGCGGCGTCACCGCCttccacg                       >  2:363932/1‑283 (MQ=11)                                                                                                                                                                                                                                       gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCa                                                                                                                                                             >  2:51156/1‑152 (MQ=35)                                                                                                                                                                                                                                              tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATc                                                                                                                                                                         >  2:64739/1‑133 (MQ=32)                                                                                                                                                                                                                                              tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGA‑Tgc                                          <  1:387304/259‑1 (MQ=39)                                                                                                                                                                                                                                                        aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGa                                                                                                                                                                                                              >  1:542313/1‑87 (MQ=33)                                                                                                                                                                                                                                                        aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCCTCAGGCAGTCGGCACCGTTGCCGGA‑TGCGGCGTAAACg                                >  2:387304/1‑259 (MQ=38)                                                                                                                                                                                                                                                        aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGA‑TGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAt  >  1:104018/1‑289 (MQ=38)                                                                                                                                                                                                                                                                  cAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAg                                                                                                                                                                                                      <  2:491727/85‑1 (MQ=33)                                                                                                                                                                                                                                                                                ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGA‑TGCGGCGTAAACGCCTTATCCGGCCTACGGa              >  1:255955/1‑253 (MQ=35)                                                                                                                                                                                                                                                                                ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGA‑TGCGGCGTAAACGCCTTATCCGGCCTACGGa              <  2:255955/253‑1 (MQ=35)                                                                                                                                                                                                                                                                                 gCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGA‑TGCGGCGTaa                                   <  2:536114/231‑1 (MQ=18)                                                                                                                                                                                                                                                                                 gCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGA‑TGCGGCGTaa                                   >  1:536114/1‑231 (MQ=18)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                               AGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGC‑GTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGA‑TGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAT  >  NC_000913/4325765‑4326300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑