New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1774019 =49 (1.210)5 (0.130) 5/268 4.4 10.0% coding (231/867 nt) ydiB quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
?NC_000913 1774066 = 44 (1.150)coding (278/867 nt) ydiB quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAAGCGGGATTGCCATTTACCTATATGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAA  >  NC_000913/1773882‑1774157
                                                                                                                                         |                                                                                                                                          
aaaGCGGGATTGCCATTTACCTATATGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGCATTTGTGAGACGATTCCGAAAAAACAAGTGGAGTGTGAATATATtga                                                                                                                                       >  1:512554/1‑143 (MQ=255)
 atgctggATTGCCATTTATCTATATGTCTTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAATAGCCCTTAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTtgat                                                                                                                                      <  1:196429/138‑1 (MQ=255)
   gCGGGATTGCCATTTACCTATATGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTTTCGATGCCGAACAAACAACAAGAGAGTGAATAAGAtgatga                                                                                                                                    >  2:595845/1‑143 (MQ=255)
     gggAGTGCCATCGACCGACACGGCCCTCGAAGCGGACACCGACAGCTTCCCTGCAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAAt                                                                                                                                  <  2:539862/143‑1 (MQ=255)
             cATTTACCTATATGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAACAGCACTCTCAATGCGCGGGACTGGTGCGTCGGTGCCGAACAACCACCTTGCGTGTGAATATTATGATGAAATAACACCa                                                                                                                          >  2:268611/1‑143 (MQ=255)
             cATTTACCTATATGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAGCTGGTGTATCGATGCCGCTCGTGTCACTGGCGTGTGTCTATGTTGATGAGTTaaaaaca                                                                                                                          >  2:228928/1‑138 (MQ=255)
             cATTTACCTATATGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACAGGCGTGTGAATATGTTGATGAATTAccacca                                                                                                                          >  1:216606/1‑143 (MQ=255)
             cATTTACCTATATGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGCAACTGGCGTATCGATGCCGAACAAACACCTACCAGGGGAAGCTGTTGATGAATTAACACCa                                                                                                                          >  1:471241/1‑143 (MQ=255)
                     tataTGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGGCCGCAAAACGCCCGGAACAGGTGTATCGATGCCGAACAAACAACTGGCGTGAGAATATGTTGATGAATTAACACCAGCTGCCaa                                                                                                                  >  2:693135/1‑143 (MQ=255)
                      ttatGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCaaa                                                                                                                 <  1:7516/142‑1 (MQ=255)
                      atatGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACAAGCTGCCaaa                                                                                                                 >  1:302995/1‑143 (MQ=255)
                        atGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACt                                                                                                               >  1:371505/1‑143 (MQ=255)
                        atGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGACGAATAAACAACAGCAACCAAAca                                                                                                               >  2:220032/1‑142 (MQ=255)
                             cTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACtggtgg                                                                                                          >  2:242884/1‑143 (MQ=255)
                                cGAAGTGGATAACGATAGCTTTCCTGGAGCAAATGAAGGAATAAAAAGCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGAAGAATTAACAACAGCTGCCAAACCGGAggggg                                                                                                       >  1:395351/1‑143 (MQ=255)
                                             gATAGCTTTCCTGGAACAAATGAAGGATTAAAAGCCCTCAAAATACGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCAt                                                                                          >  1:52272/1‑143 (MQ=255)
                                                      ccgtgggCAATTGAAGGACTAAAAGCCCTGAAAACGCGCGGAACTGGTGGATCGATGCCGAACAAACGCCTGGCGCATGAATATGTTGTTGAATTAACACAAGCGGCCAAACTGGTGGGGGCCATCAACACCATCGTTAatga                                                                                 <  2:655058/137‑1 (MQ=255)
                                                       cTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACCCCAGCTGCCAAGCTGGGGGGGTCCATCCACACCATCGTTaataat                                                                                >  2:122032/1‑143 (MQ=255)
                                                        tGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACACACAACTGGCGTGTGAATATGTTGCTGACTTAACACCAGCTGCCAAACTGATGGGGACCATCAACAACGTCGTTAatgatg                                                                               >  2:396918/1‑143 (MQ=255)
                                                             cAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCCGCTGCCAAGCCGGTGGGGGCCATCAACACCATCGTTAATGATGATGGc                                                                          >  2:227812/1‑143 (MQ=255)
                                                                ttGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATAGCTAt                                                                       >  2:203068/1‑143 (MQ=255)
                                                                     ggATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCg                                                                  <  1:455678/143‑1 (MQ=255)
                                                                     ggATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGCTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCg                                                                  >  2:532348/1‑143 (MQ=255)
                                                                       aTTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTg                                                                <  2:371505/143‑1 (MQ=255)
                                                                        ttAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTgg                                                               >  1:578284/1‑143 (MQ=255)
                                                                            aaGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCtat                                                           <  2:395351/143‑1 (MQ=255)
                                                                                 cTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGGATTATCACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAacac                                                      >  2:518517/1‑143 (MQ=255)
                                                                                 cTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGCCCCTCAACACCAACGTTAATGATCATGGCTATCTGCGTGGCCATAacac                                                      >  1:588281/1‑143 (MQ=255)
                                                                                  tCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACAcc                                                     <  1:459812/143‑1 (MQ=255)
                                                                                  tCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGGGTGAATATGAGGATGAATTAACACCAGCTGCCAAACTGGGGGGGGCAATCAACACCAGCGATAATGATGATGGCCATCTGCGAGGCGATAACAcc                                                     >  1:72196/1‑143 (MQ=255)
                                                                                   taatttgtgcgcAAGTGGTGGATCGATGCCGAACAAACAACTGGCGTGTTAATATTTTGATGAATTAACACCATCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCg                                                    <  1:170210/135‑1 (MQ=255)
                                                                                       aTGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATCATGATGGCTAACCGCGAGGCTAcaccacgcccgg                                                >  1:600398/1‑137 (MQ=255)
                                                                                            gagAACTGGTCTAGCGCTGCCCAACACACAACTGGCGTGTGAATATGGTCAAAAATCACTACCATCTCCCAAACTCGTTGCGGCCATCAACACCATCGTTAATGATTATGGCTATCTGCGTTGCTATAACACCGACGGCACgg                                           <  1:433409/141‑1 (MQ=255)
                                                                                               aaCTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACACACGCCACGGGcc                                        >  1:687222/1‑143 (MQ=255)
                                                                                                  tGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCACACTGGTGGGGGCCATCACCACCATCGTTAATGATGATGGCTATCAGCGTGGCTAAAACACCGACAGCACGGGACata                                     >  2:576967/1‑143 (MQ=255)
                                                                                                  tGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGAGGGCTATCTGCGTGGCTATAGCACCGACGGCATGGGCCata                                     >  2:576805/1‑143 (MQ=255)
                                                                                                       tATCGATCCTGAACAATCAACTGTCGGGTGATTATTTTTATTAATTAACACCATCTGCCAAACTGGTGGGTGCCATCAACACCATCGTTATTGATGATGGCTATCTGCGTGTCTATAACACCGACGGCACGGGCCATATTcgc                                <  1:61581/143‑1 (MQ=255)
                                                                                                          cgaggacGAACAAATAACGGGGGTGTGAATATGTTAATGAATTAAAACCAGGTGCCAAACTGGTGGGGGGCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGcc                             <  1:227189/137‑1 (MQ=255)
                                                                                                                  ggcaagcgacTGGCGTGTGGATAGGTTGATGATTTAACACCAGCTGCCAAACTGGTGGTGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAaga                     <  1:725097/135‑1 (MQ=255)
                                                                                                                     aaacaaCTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAagagag                  >  2:135132/1‑143 (MQ=255)
                                                                                                                     aaacaaCTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATCACagagcg                  >  2:500718/1‑141 (MQ=255)
                                                                                                                      aacaacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACGCCGCCGGCACGGGCCATCTCCGCGCCtttccgctgtgc                 >  2:213290/1‑134 (MQ=255)
                                                                                                                       tcgacgtgCGTGTGAATATGTTGATGAATTAACACCATCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCg                <  1:242884/136‑1 (MQ=255)
                                                                                                                           gtGGAGTGTGAATATGTAGATGAATTAATACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCCGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGttt            <  2:573835/142‑1 (MQ=255)
                                                                                                                            tGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAAGGATGATGGCTATCTGCGTGGCTATAACACCGACGTCACGGGGAATATTCGGCCCATAAACGAAACCGGtttt           >  2:501851/1‑143 (MQ=255)
                                                                                                                              gCGTGTGAATATGTTGATGAATTAGCACCAGCTGCCGATCTGGTGGGGGCCATCAACACCATCGTTAATGATAATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGTCATTAAAGAGAGCGGTTTTGa         <  1:220032/143‑1 (MQ=255)
                                                                                                                                gtgtGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATCTTCGCGCCCTTAAGGAGAGCGGTTTTGata       >  1:231076/1‑143 (MQ=255)
                                                                                                                                gtgtGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGAGATAACACCGACGGCACGGGCCAAATACGAGCCAGCAAAGAGAGCggcgtggatc       >  1:528290/1‑135 (MQ=255)
                                                                                                                                     aaTATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACCGCACGGGCCATATTTGGGCGATTAAAGAGAGCGGTATTGATATCaaa  >  2:22520/1‑143 (MQ=255)
                                                                                                                                         |                                                                                                                                          
AAAGCGGGATTGCCATTTACCTATATGGCCTTCGAAGTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAA  >  NC_000913/1773882‑1774157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.