Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA94.9% 91.4 / NA 41P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (16/21);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC  >  NC_000913/4184405‑4184675
                                                                                                                                         |                                                                                                                                     
tgCGGTTTAACGCCGTTTCCAGTCTGGTGACAAGATGGTAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                  <  2:485033/142‑1 (MQ=255)
        aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACACCGGAAGACATAGTACTGAAACAGCTGGGCTTAcag                                                                                                                          >  2:1154/1‑141 (MQ=255)
          cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGCTGAAAACCGTACGCCGGTGCACATCGTACTGCCCCCGCTCCGCGTCCCGtc                                                                                                                        >  2:76052/1‑143 (MQ=255)
              gTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                    <  1:69555/143‑1 (MQ=255)
               gatgcAGCCGGGGGACAAGAGGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                   <  1:245437/139‑1 (MQ=255)
                    aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGCTGTGCCTTACGCTGAAAACGGTACGCCGGTAGACATCGGACTGAACCTGCTGGGCGTACCGTCTCGTATGAAc                                                                                                              >  2:355561/1‑143 (MQ=255)
                     gCCTGGTGACAAGATGGCAGGTCATCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACCGTCCGACGGTCGACCTCGTCCTGCACCCGCTGGGCGCACCGCCTCCTATGAAcc                                                                                                             >  1:129326/1‑142 (MQ=255)
                                 gctgtgaGGTCGTCACGGTAACAAGGGTGTAATTGCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcc                                                                                                 <  1:205795/137‑1 (MQ=255)
                                  aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGCACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                >  2:469234/1‑143 (MQ=255)
                                       agctcctcACGGCAACAAGGGTGTAATTTCTCAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                           <  2:243452/137‑1 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGGGGAATTTCTAAGATCAACCCGATCGAGTATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                           <  1:114387/143‑1 (MQ=255)
                                         gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGTATAAAACTCGGTGAGATCCCCGAAAcc                                                                                         >  1:330595/1‑143 (MQ=255)
                                              cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAATACGGTACGCTGGTAGTCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                    <  2:67419/143‑1 (MQ=255)
                                               aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTACCGTCTCGTATGAACATCGGACAGATCCTCGAAACCCaacag                                                                                   >  2:494186/1‑139 (MQ=255)
                                                 ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACACCGGTCAGATCCTCGAAACCCACCAggg                                                                                 >  2:621601/1‑143 (MQ=255)
                                                  atAACCAGGGTGTAACTGCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTTCGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGt                                                                                <  1:207291/142‑1 (MQ=255)
                                                    aagaaGGGTGGAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                              <  2:703989/140‑1 (MQ=255)
                                                            tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCTGGGGATCGCTGCGa                                                                      >  2:244632/1‑143 (MQ=255)
                                                            tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                      >  2:511917/1‑143 (MQ=255)
                                                              ttaTTTTTAATATCTTCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                    <  2:634549/141‑1 (MQ=255)
                                                               aaTTTCTAAGATCAACCTTATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                   <  2:42700/143‑1 (MQ=255)
                                                               aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCTGTAGACATTGTTTTGGTCGAGCAGAGCGAACCGGCACGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                   <  2:268495/143‑1 (MQ=255)
                                                                 tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTACGAAAGGt                                                                 >  1:1784/1‑143 (MQ=255)
                                                                  ttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCTCGAGACCCACCTGGGTCTGGATgagtttatat                                                                >  2:226799/1‑134 (MQ=255)
                                                                   tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCACCTGGGTATAGCTGCAAAAGatat                                                               >  2:680312/1‑143 (MQ=255)
                                                                    cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCACGGATGAACATCGGTCAGATCCTCGAAACCCACCTCGGTATGGGTGCGAAAGGTATc                                                              >  1:336531/1‑143 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTCTGAACCTCGGTCAAATCCTCGAAACCCACCTGGGTATGGCTGCGAAAAGTATCg                                                             >  2:473127/1‑143 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACCGAACCAGCCGGGCGTACAGACTCGTATGAACATCGGTCAGGTCCACGAAACCCCCCTCGGTACTGCTGCGAACGGTCTCg                                                             >  1:549395/1‑143 (MQ=255)
                                                                          tCAATCCGATAGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACATCGTACTGAACTAGCTGTGCGTACCGTCTCCTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                        <  1:205033/143‑1 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTGAGATCCTCGAAGCCCACCTGGGGATGCCTGCGAAAGGTATCGGCGActa                                                      >  1:373838/1‑141 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACAAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGagca                                                      >  1:262476/1‑140 (MQ=255)
                                                                               ccGATCGAAGATATGCCTTACGATGAGAACGGTGCGCCGGTAGACATCGTACTGGACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                   <  1:715147/143‑1 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCTGGTAGACATCGTACTGTACTAGTTGGGCGTAGCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                               <  2:356695/143‑1 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                               <  2:112177/143‑1 (MQ=255)
                                                                                             gCCGTACGATGAAAACGGCACGCCGGTAGACATCGAACTGGACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa                                     <  1:42846/143‑1 (MQ=255)
                                                                                                               tACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                   <  1:365935/143‑1 (MQ=255)
                                                                                                                       tAGTCATCGTACGGAACCAGTTGGGTGTACCGTCTCGTATGAACGTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg           <  1:638324/143‑1 (MQ=255)
                                                                                                                             tcccaCTGACCCGGCTGGGCGTGCCGTCTCGTATGAACAGCGGTCAGATCCTCGAAGCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa     <  1:621601/139‑1 (MQ=255)
                                                                                                                              cGTACTGAACCAGCTGGGCGTACCGTCTCGTGTGGCCAGCACACAAAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt    <  2:689874/143‑1 (MQ=255)
                                                                                                                                tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCGGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCACCGCCGTGGGGAAACAGCGGCAAGACGTCGCTCAACTGTGCGAGtta  >  2:3404/1‑142 (MQ=255)
                                                                                                                                caCTGAACCATCTGTGCGTACCGTCTCTTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc  <  2:405329/142‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC  >  NC_000913/4184405‑4184675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑