| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 95.0% | 95.1 / NA | 40 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (18/20); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
TAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT > NC_000913/2132655‑2132922 | tAGCGGGTAAACAGCTGATATTGTGTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGTAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:294926/143‑1 (MQ=255)tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGCGATCTGCACTTCATCCAGCCTGACATGCAGCCTCTGCGACGc > 1:603717/1‑143 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAGGTGGAAGTACCCGGAGCCTTTTTTCCGTGCCGTGGTGATCTACCTTACACAGAAACTGACATACAAGATCtacataaacactt > 2:5937/1‑131 (MQ=255) aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATGTGCAGGCTCTGCGTCGCCTCTTCTACttt > 2:506801/1‑143 (MQ=255) aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGAAAGAACTGCGTCGCCACTTATACttg > 2:422252/1‑142 (MQ=255) atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATACGCCGGCTCTGCGTCGCCTCGTCTCCTTTCCACg > 2:634554/1‑143 (MQ=255) ttttGCTGGCGTGGTTGTATTTGTCTTTGTGCTTGTGGTAAAAGTGGAAGTACCCGGAGAAGGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCg < 2:345141/143‑1 (MQ=255) tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGACAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTACGTGGCCTCTTCTACTATCGACggaccga > 1:393374/1‑138 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc < 2:260008/143‑1 (MQ=255) gcgcTGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGTGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc < 2:582421/143‑1 (MQ=255) gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 2:612339/1‑136 (MQ=255) gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc < 1:612339/136‑1 (MQ=255) ttCTTTGTGCTTGCGGTAGAAGCGGAAGTACCCGGAGGAGCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa < 2:43355/142‑1 (MQ=255) cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACCGTTCGCCCGACTccaccacccacccccgg > 1:554816/1‑134 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGTAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa > 1:263991/1‑143 (MQ=255) gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCACGGTCCTCTCCCACCATCCGCCggcaca > 1:111962/1‑139 (MQ=255) ttGCGGTAAAATTGTAAGTACTCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 2:503417/143‑1 (MQ=255) ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc > 2:248305/1‑143 (MQ=255) ttGCGGAAAAAGAAGAAGGAACCGGAAAATAGTTTCGGAGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 2:157798/143‑1 (MQ=255) gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACAATCCGCAGGAAGCTGTCGt > 2:545392/1‑143 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCCTCCGCACGACGATGTCGGAAt > 2:732121/1‑143 (MQ=255) aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTGCTCCACCACCATCCGCAGGCAGATGAAGTAACCCcgt > 1:650640/1‑143 (MQ=255) aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt > 2:310175/1‑143 (MQ=255) taaGTACCCGGAGAGTTTTTTCGGTGACTAGGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTGCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCg < 1:670228/142‑1 (MQ=255) cgttcccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCt < 1:491273/139‑1 (MQ=255) gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCAGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACAATCCGCAGGAAGCTGTCGTAATCATGTACCGcatcaagctcga > 1:171011/1‑132 (MQ=255) tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc < 1:603678/143‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCGCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 1:310175/143‑1 (MQ=255) ttttCGGAGACGAAGTGATCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 2:442070/143‑1 (MQ=255) ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTCCGTCGCCTCTTCTTCTGTCGACGGTTCGCCGTACTCCACCGCCATCCCCTGGAAGATGTCGTATTCCTTTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt < 1:346220/143‑1 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTNATCCTGTGCCGCTTTCAGTTCGGCATCTCACAAacacacaaagaa > 1:383161/1‑135 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGCAAGATGTCGTAATCCTCTGCCGCGTTTGGTTCGGTATCGAGCAAGCACTCGTTGagca > 1:146130/1‑140 (MQ=255) tGCATTTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg < 1:288206/143‑1 (MQ=255) gttctcgtcGTGATTGATATGCAGGCTCTGCGGCTCATCTTCTACTGTCCGCGGTTCGCCGTACTCCACCGCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc < 1:570959/135‑1 (MQ=255) tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTCTCAGTGCGGCATCGAACCAACACTCCTTGAGACGCCAt > 1:118856/1‑143 (MQ=255) cgcgattggtgcgCAGGCTCTGCGTCTCCTCCTTTACTTTCTACGGTTCGCCGTGCTCCACCACCATCCGTAGGAATATGTCGTAATCCTGTGCCGCTTTCCGTTCGGTATCGAACAAACCCTCTTTGAAACGCCATGCCCAg < 1:175502/131‑1 (MQ=255) cGTGATTGATTTGCATGCTTCGCGTCTCCTCTTCTACTTTCCACGGTTCGTCGTGCTCCACCACGATCCGCACGAAGATTCCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg < 1:308173/143‑1 (MQ=255) ttgacATGCAGGCTCTTCGTCTCTTCTTATACTGTCCACGTTTCGCCTTACTCCACCACTATCCTCATGAAGATTTCGTAATCCTGTGCCGCTGTCAGTTCGGTATTGAACAAACACTTTTTGAAACGCCATGCCCAGGTaaa < 1:731206/143‑1 (MQ=255) atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCCTATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAACGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc > 2:225431/1‑143 (MQ=255) attcAGGCTTTGCGTCGCCTCTTCTCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt < 1:248305/140‑1 (MQ=255) | TAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT > NC_000913/2132655‑2132922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |