| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,414,035 | A→C | T190P (ACC→CCC) | ackA → | acetate kinase A and propionate kinase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,414,035 | 0 | A | C | 97.8% | 125.0 / ‑6.7 | 46 | T190P (ACC→CCC) | ackA | acetate kinase A and propionate kinase 2 |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (21/24); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGTATTTGACACCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTAACCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGA > NC_000913/2413905‑2414174 | cgCTGTTTTTGACACCGCGTTCAACCATACGATGCCGGAAGAGGCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCgg < 1:383477/142‑1 (MQ=255) tGTATTTGACACCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTCCCTTACAACCTGTACAAAGAGCACGGCATCCATCGTTACGGCGCGCACGGCACCAGCCCCCTCTATGTACCCCAGGCAGCCGCac > 1:179228/1‑142 (MQ=255) ttGACACCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATg < 2:635498/143‑1 (MQ=255) gACATCGCGTTCCACCATACTATGCCGTAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCt < 1:490419/143‑1 (MQ=255) acacCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTg < 1:450476/143‑1 (MQ=255) acCGCGTTCCACCAGACTATGCCGGAAGAATCTTACATCTACGCCCTGCCTTACAACCTGTACAAAGAGCACAGCATCCGTCGTTACAGCGCGCACGTCACCAGACACTTCTATGTACCCCAGAAAGCGGCAAAAATGCTaaa > 1:288608/1‑143 (MQ=255) tgTTCCATCAGTCTATGTCTTAATAGTTTTTTCTCTACTTCCTGTTTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACaaa < 2:543782/142‑1 (MQ=255) cgTCCCACCAGACTATGCCGGAAGCGTCTTACCTCTACGCCCCGCCTGACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCATGAAGCGGCAAAAATGCTGAACaaa < 2:690345/143‑1 (MQ=255) ccatcaGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCgg < 2:72846/143‑1 (MQ=255) ccaccaTACTATGCCGCAACACTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCgg < 2:712874/143‑1 (MQ=255) ccaccaGACTAGGCCGTAGGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCgg < 1:199747/143‑1 (MQ=255) accaGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGAAATAAGTAATAACGGCGCGCACGGCACCAGCCACTTCTATGTACACCAGGAAGCGGCAAAAATGCTGAACAGACCGGTa > 2:111376/1‑143 (MQ=255) caGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCCCCAGCCCCTTCTATGTACCCCAGGACACGGCAAAAATGCTGAAAAAACCGGAaga > 2:660557/1‑143 (MQ=255) caGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACCTCTATGTACCCCCGGACGCGGCGACAGTGCTGAACAAACCGGTaaa > 2:289650/1‑141 (MQ=255) caGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGAGCGCACGGCACCAGCAACTACTATGTACCCCAGGAAGCGGCAAAAATGCAGAACAAAACGGTaga > 2:367458/1‑143 (MQ=255) tgtGCCGTAAGAGTCGTACCTCTGCGGCCTGCCTTACAACCTGTGCAAAGAGCACGGCATCCGTCGTTACGGCGCCCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAGATGCTGAACAAACCGGTAGAAGAAc < 1:555262/141‑1 (MQ=255) tATGCCGGAAGAGTCTTACCACTACGCCCTGCCTTACAACCTGTAAAAAGAGCACGGCATCCGTCGTTACGGAGCGCACGGCACCAGCCACTTCTATGTACCCCAGGACGCGACAAAGATGCGGAAAAAACCGCACGAAGAAc > 1:303679/1‑143 (MQ=255) tGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTg < 2:295116/143‑1 (MQ=255) gtcagAAGAGTCTTACCTCTACGCCCTGCCGTACAACCTGGACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGa < 2:432731/139‑1 (MQ=255) gAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGCATTTAGAACAAACTAATAGAAGGGCACCGGCCACATCTATGTACCCCAGGAAGCGGCAAAAAGGCTGAACAAACACGTAGAAAAACTGAAcac > 1:516188/1‑142 (MQ=255) gTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGCCCCCAGCCACTTCTATGTACCCCAGGAAACAACAAAAATACAGAACAAAAAGATAGAAGAACTGAACAtcatca > 2:87364/1‑143 (MQ=255) cTTACCTCTACGCCCTGCCTTACTACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACCGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCAcc < 1:526579/143‑1 (MQ=255) ttACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCCTTCCGGCGCGCACGCCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGATGAACAAAACGGTAGAAGAACCGAACAACATCAccc > 1:83173/1‑142 (MQ=255) cGCCCTGCCTTACTACCTGTCCAAATAGCACAGTATTCTTTTTTACGTCGCGCACGGCACCAGCCACTTATATGGACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTgg < 2:602378/143‑1 (MQ=255) cGCCCTGCCGTACAACCTGTAGAAGGAGCAAGGCAGCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTgg < 2:563182/143‑1 (MQ=255) cTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCGGAACAAACCGGTAGAAGAACTGAACATCATCACCTGACACCCgggagc > 1:358815/1‑140 (MQ=255) cTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTAAGTACCCCAGGAAGCGGCACAAATGCTGAACAACCAGGTAGCAGAGCTGAACACCATCACCAGCCAccacgtcaa > 1:358819/1‑136 (MQ=255) tGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACCTCTACGTACCCCAGGAAGCGGCACAAATGCTGAACAAACCGATAGAAGAACTGAACATCATAACCTGCCACCAGAGCAAc > 2:581883/1‑143 (MQ=255) cTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAACCATGCTGAACAAACCGGTAGAAGCACTGAACATCATCACCTGCCACCTGGGCAACggc > 1:34022/1‑142 (MQ=255) ttACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCAGCAGCCACTTCTATGTACCCCAGGAAGCGGCAAACATGCTGAACAACCCGGTCGAACAACTGCACATCATCCACTGCCACCTGTGCACTggtg > 2:589538/1‑143 (MQ=255) aCAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACggtggt < 2:156586/143‑1 (MQ=255) aaGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCCCCTGGGCAACGGCGGCTCCGTTTCTGCt > 1:454611/1‑143 (MQ=255) aaGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGc > 1:701649/1‑122 (MQ=255) aaGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGc < 2:701649/122‑1 (MQ=255) ggCACGTCATCCGTCGTTACCGCGCGCACGGCCCCAGCCACTTCTATGTAACCCAGGAAGCGGCAAAAATGCTGAACAAGCCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATc < 1:60189/142‑1 (MQ=255) agCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGCGCAACGGCCGTTCCGTTTCTggcata > 1:64505/1‑138 (MQ=255) gCACGGCCTCCGTCGTTACGGGGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATcc < 1:111376/143‑1 (MQ=255) gCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTAGGCAACGGAGGATCCGTATCTGCTATCCGCAAc > 2:687496/1‑143 (MQ=255) cgtggtTACGGCGCGTACGGCACCTGCGGCTTCTATGTACCCCAGGAAGCGGCAAAAGTGCTGAACAAGCCGGTAGAAGAACTGAACATCAGCATCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTaa < 1:351157/143‑1 (MQ=255) gtagtTACGGCGCGCACGCAACCTGCCACATCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTaaa < 1:596332/140‑1 (MQ=255) aCGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGCAGAACTGAACATCCTCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCCGCTATCCGCAACGGTCAATGCGtc > 1:124972/1‑142 (MQ=255) cACCAGCCACTTTTATGTACCCCAGGAAGCGTCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATgg < 2:316010/143‑1 (MQ=255) ccAGCCACTTCTATGTGCCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGc < 1:660557/143‑1 (MQ=255) ccAGCCACGTCTATGTACCCTAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGc < 1:367458/143‑1 (MQ=255) ccACTTCTATGGTCCCCAGGAAGCGGCAAAAATGCTGAACAAACCTGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGa < 1:366522/143‑1 (MQ=255) gTACCCCCGGCAGCGGCAAAAAAGCTGAACAAACCGGTCGAAGAAGTAAACATAATCACAAGACACCTGGGCAACGGTGGTTCCGTTTCTGCTCACCGCCACGGTGAAAGCATTGCCGCCACTATGGACATGagacacgcgga > 1:134365/1‑133 (MQ=255) | TGCTGTATTTGACACCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTAACCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGA > NC_000913/2413905‑2414174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |