Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,542	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,542	0	C	A	100.0%	232.9 / NA	80	P1100Q (CCG→CAG)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (44/36);  total (44/36)

CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4184403‑4184679                                                                                                                                            |                                                                                                                                          cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGCAAGACATCGCACTGAACCAGCt                                                                                                                                        >  1:357149/1‑143 (MQ=255)      ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATAGCACGGAACCAGCTGGGCg                                                                                                                                   >  1:488661/1‑143 (MQ=255)      ggTGAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                   <  1:868365/143‑1 (MQ=255)       gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                  >  1:126155/1‑143 (MQ=255)       gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCAGGGAGt                                                                                                                                  >  2:33491/1‑143 (MQ=255)       gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                  >  2:1051356/1‑143 (MQ=255)        tcacactccGTATCCAGCCTGTGGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                 <  1:1070653/136‑1 (MQ=255)          aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGGAcc                                                                                                                               >  2:1105736/1‑143 (MQ=255)           aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCgaccag                                                                                                                              >  2:34758/1‑138 (MQ=255)            aCGCCGTATCCAGCCTGGTGACAAGATGGAAGGTCAGAACCTAAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                             <  1:548614/143‑1 (MQ=255)             cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtc                                                                                                                            >  1:398703/1‑143 (MQ=255)              gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                           >  2:1168089/1‑143 (MQ=255)              gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGCGTAATTTCTAAGCTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCACACTGAACCAACTAGGCGCACCgacc                                                                                                                           >  1:596021/1‑140 (MQ=255)               ccGGATCCAGGCTGGTGACAAGAGGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                          <  1:763527/143‑1 (MQ=255)                 gTATCCAGGCTGGTGACAAGATGGCAGGTCGTCGCGGTAACAAGGGTGTAATTTCTAAGACCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                        <  1:1247577/143‑1 (MQ=255)                  tATCCAGCCTGTTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                       <  1:332257/143‑1 (MQ=255)                    tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGAAATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGGATg                                                                                                                     >  1:345403/1‑143 (MQ=255)                      cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTCACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATACGCCTTACCATGAAAACGGTACGCCGGTAGACGTCTCACACAACCAGCTGGGCGTACCGTCTCGTCTgca                                                                                                                   >  1:257825/1‑141 (MQ=255)                           cggTGACAAGACGGCAGGTCGTCGCGGTAACAAGGCTGTGATTGCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                              <  2:281052/142‑1 (MQ=255)                            ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCgg                                                                                                             >  1:136089/1‑143 (MQ=255)                            ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCgg                                                                                                             >  2:1250343/1‑143 (MQ=255)                              tGACAAGATGGCGCGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGCAAACGGTACGCCGGTAGACATCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                           <  1:287807/143‑1 (MQ=255)                              tGACAAGATGGCAGGCCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                           <  1:60690/143‑1 (MQ=255)                                aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGAACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACGGTCTAGCATGAACAGCGGTCAg                                                                                                         >  1:1032856/1‑143 (MQ=255)                                     aTGGCAGGTCGTCACGGTAACAAGGGTGTGATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                    <  1:225586/143‑1 (MQ=255)                                      tGGCGGGTCGTCACGGCAACAAGGGTGTAATTTGTAGGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGCACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                   <  1:322722/143‑1 (MQ=255)                                      tGGCACGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCCACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                   <  2:833402/143‑1 (MQ=255)                                       ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCg                                                                                                  >  2:72274/1‑143 (MQ=255)                                        gCAGGTCGTTACCGTAACAAGAGTGTAAGTCCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCATCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                 <  2:42977/143‑1 (MQ=255)                                          aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                               <  2:951957/143‑1 (MQ=255)                                          aGGTCGTCACGGTAACAAGGGTCTGATTTCTAAGATCAACCCGATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                               <  1:303570/143‑1 (MQ=255)                                           ggTCGTCACGGTAACAAGGGTGTAAGTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                              <  2:1167482/143‑1 (MQ=255)                                                tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATGGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACACAcc                                                                                         >  2:637228/1‑143 (MQ=255)                                                tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCCTCTCGTATGAACATCGGTCAGATCCTCCCAACCCAcc                                                                                         >  1:1230992/1‑143 (MQ=255)                                                   cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                      <  1:486583/143‑1 (MQ=255)                                                    ggTAACAAGGGTGTAATTTCTAAGATAAACCCGGTCGTGGTTTTTCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgcg                                                                                     >  2:530911/1‑141 (MQ=255)                                                     gTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGt                                                                                    >  2:958028/1‑143 (MQ=255)                                                       aaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAAGACCATCGGGCAGATCCTCGAAACCCACCCGGGGAt                                                                                  >  1:1240688/1‑143 (MQ=255)                                                        aCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAACAGCTGGGCGTACCGTCTCGTATGAACACCGGTCAGATCCTCGAAACCCCCCTGGGTATg                                                                                 >  1:125014/1‑143 (MQ=255)                                                          aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGACCATCGGTCAGACCCTCGAAACCCACCCGGGAATgga                                                                               >  1:483216/1‑142 (MQ=255)                                                             ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGGAACCCACCCGGGGCTGGCCGc                                                                            >  1:1061364/1‑143 (MQ=255)                                                               tgtgaattCTAAGATCAACCCGATCGTAGATATGCCTTACGATGCCATCGGTACGCCGGTAGACATCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                          <  1:33491/137‑1 (MQ=255)                                                               tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGGAAACGGTACGCCGGTAGACATCGTACTAGACCAGCTGGGCGTACCGTCTCGTATGACCATCGGTCAGATCCTCGCAACGCACCGGGGGATGGGCGCGa                                                                          >  1:625762/1‑143 (MQ=255)                                                                gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                         >  1:854583/1‑143 (MQ=255)                                                                 tAATTTCTACGATCAACCCGTTCGAAGCTAGGCCTTACGATGATAACGGTACGCCGGTAGACATCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                        <  1:357565/143‑1 (MQ=255)                                                                 tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                        <  2:1052491/143‑1 (MQ=255)                                                                  acttgttAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACTCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                       <  2:963705/137‑1 (MQ=255)                                                                  aaTTTCTAAGATCAACCGGATCGATGATATGCCGTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                       <  1:297680/143‑1 (MQ=255)                                                                   aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAACGTAGTGAACCAGCTGGGCGTACCGTATCGTAAGAACATCGGACAGCACATCAAAACCCGCCCGGGGACAGGTGCGAAAgg                                                                      >  1:1242183/1‑143 (MQ=255)                                                                    tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCAACCTGGGTATGGCTGCGAAAggg                                                                     >  1:119768/1‑142 (MQ=255)                                                                      tCTAAGATCAACCCGATCGAAGATATGCCTTACGGTGAAAACGGTACGCCCGTAGACATCGCACTGAACCAGCTGGGCGTACCGTCCCGTCGGAACACCGATTCTTTCCCCGCAACCGCCCGGGGTATGGCAGCggagggaat                                                                   >  1:549985/1‑135 (MQ=255)                                                                         aaGATCAACTCTTTCGAATATATGCCTTATGATGAAAACAGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATGCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCgg                                                                <  2:866818/143‑1 (MQ=255)                                                                         aaGATCAACCCGAGCGAAGATCTGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCgg                                                                <  1:93232/143‑1 (MQ=255)                                                                          aGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                                               >  2:136320/1‑143 (MQ=255)                                                                           gATCAACCCGATCGAAGATATGCCTTACGATGATAACGTGGCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAATGGTATCGGCg                                                              >  2:585793/1‑143 (MQ=255)                                                                           gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCg                                                              >  2:971427/1‑143 (MQ=255)                                                                            agcAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                             <  2:896413/141‑1 (MQ=255)                                                                            aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGGAGCACACCTGGGGATGCCTGGGAGAGGtgaagacgc                                                             >  1:991094/1‑135 (MQ=255)                                                                             tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                            >  2:699405/1‑143 (MQ=255)                                                                                 cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                        >  2:978016/1‑143 (MQ=255)                                                                                 cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                        >  1:531132/1‑143 (MQ=255)                                                                                  cggATGGACGATATGCCTTACGCTGAACACGGTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                       <  1:832856/141‑1 (MQ=255)                                                                                  ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                       <  2:761979/143‑1 (MQ=255)                                                                                    gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGCATGGCTGCGAAAGGTCTCGGCGACATGATCa                                                     >  2:1211878/1‑143 (MQ=255)                                                                                     aTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAGCCAGCTCGGCGTACCGACTCGTATGAACATCGGTCAGATCCTCCAAACCCACCGGGGTCTGCCTCGGAAAGGGATCGGCGACAAGACCaa                                                    >  1:820979/1‑143 (MQ=255)                                                                                      tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTCCCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCTGGGTGTGGCTGCGACACATATCGGCGACCAGacaacc                                                   >  1:363160/1‑138 (MQ=255)                                                                                      tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                   <  1:236911/143‑1 (MQ=255)                                                                                       cGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGCTCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACg                                                  <  2:652687/143‑1 (MQ=255)                                                                                          aGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                                               <  2:762649/143‑1 (MQ=255)                                                                                                 ccTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaa                                        >  2:69888/1‑143 (MQ=255)                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       <  2:488661/143‑1 (MQ=255)                                                                                                  cTTACGAGGAAAACGGTGCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       <  2:28719/143‑1 (MQ=255)                                                                                                              ccGGTACGCCGGTAGACATCGTACTGCACCAGCTGGGCGTACCGCCTCGTATCAACATCGGTCAGACCCACGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  2:685766/142‑1 (MQ=255)                                                                                                                 gTACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                        <  2:126155/143‑1 (MQ=255)                                                                                                                               aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa          <  1:699405/143‑1 (MQ=255)                                                                                                                                tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGAGaa         >  2:160008/1‑143 (MQ=255)                                                                                                                                tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGGCGAGAAACTGCGCGaa         >  1:297810/1‑143 (MQ=255)                                                                                                                                 cGTACCGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt        <  1:662751/143‑1 (MQ=255)                                                                                                                                  gTACTGAACCAGCTGGGCGTACCGTCTCGTATTAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGGCAGGGATCGCCGACAAGATCAACGCAATGCGGAAACAGGAGCAAGAAGTCGCGAAACAGCGCGCAtg       >  2:213161/1‑142 (MQ=255)                                                                                                                                       gAACCAGGTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACATGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGACGCGAAACTGCGCGAATTCATcc  >  1:1166109/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                          CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4184403‑4184679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑