Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,088,068	A→G	I597T (ATC→ACC)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,088,068	0	A	G	98.9%	303.6 / NA	94	I597T (ATC→ACC)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (48/45);  minor base T (0/1);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

ATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAG  >  NC_000913/1087932‑1088205                                                                                                                                          |                                                                                                                                          aTAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGtttt                                                                                                                                     >  2:735771/1‑143 (MQ=255) aTAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGtttt                                                                                                                                     >  1:510807/1‑143 (MQ=255) aTAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGtttt                                                                                                                                     >  2:486850/1‑143 (MQ=255) aTAGTTTTTCACTCCATTCAGTTGTAATAGGCGCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTGATTCGAAAATGGG‑TTTGGCTTTAGCCTGAGGGGGGtttg                                                                                                                                     >  2:892239/1‑142 (MQ=255)      ttttCACTCCGTTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAAtt                                                                                                                                <  1:1133788/143‑1 (MQ=255)       tttCACTCCATTCACTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAAttt                                                                                                                               <  1:220982/143‑1 (MQ=255)         tCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGATTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGa                                                                                                                             <  2:294078/143‑1 (MQ=255)         tCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGa                                                                                                                             <  2:385619/143‑1 (MQ=255)         tCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGa                                                                                                                             <  1:469039/143‑1 (MQ=255)         tCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTGTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGa                                                                                                                             <  1:1156941/143‑1 (MQ=255)         tCACTCCATTCAGTGGTAATAGGCTCATCCAGTGCGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGa                                                                                                                             <  1:162708/143‑1 (MQ=255)           aCTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGACATAGCCTGATGCTGACCCTTTTTCTGCCAGTCTTGTGCCTGTAATTCTAACATAGC‑TTTATCTTGCGGCTGCGGGGTGTGTTGAATTTGttt                                                                                                                           >  1:923486/1‑143 (MQ=255)           aCTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGAtt                                                                                                                           <  2:471937/143‑1 (MQ=255)           aCTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGAtt                                                                                                                           <  1:231459/143‑1 (MQ=255)           aCTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTGTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGAtt                                                                                                                           <  1:890424/143‑1 (MQ=255)             ttcATTTAGTTATAATAGGCTCATGCAGTGGGCGAGTTGTTGCGAAGAAAGGGCGTGATGCTGAGCTGTTTTGGGCGGGTTTTGTGGCGGGAATTCTAAAATAGA‑TTTGTGTGTATGCTGAGGGGTGTTTTTAATTTGATTgg                                                                                                                         <  1:555293/141‑1 (MQ=255)             tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGAtttg                                                                                                                         >  1:273024/1‑141 (MQ=255)             tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTgg                                                                                                                         >  1:272627/1‑143 (MQ=255)             tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTCTGTGCCTCTACTTCTAAAATAGA‑TTTGTCTTTAGCCTGACGGGTGtttttcgcttacttcg                                                                                                                         >  1:81806/1‑132 (MQ=255)               cATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGCAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTGAAATAGA‑TTTGGCTTTAGCCTGAGGGGGGTTTCTAATTTGATCGGTc                                                                                                                       >  1:1127338/1‑143 (MQ=255)                aTTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCa                                                                                                                      >  1:180579/1‑143 (MQ=255)                aTTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCa                                                                                                                      <  2:1201713/143‑1 (MQ=255)                 ttCAGTTGTAATAGGCTCATCCAGTGAGCGAGTAGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGCTTTGTGCCTGTAATTCTAGAATCGA‑TTTGTCTTTAGCCTGACGGGTGTTTTTCATTTGATTCGTccc                                                                                                                     >  1:1250507/1‑141 (MQ=255)                  tCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAAt                                                                                                                    <  2:172405/143‑1 (MQ=255)                   cAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAAtt                                                                                                                   <  2:213524/143‑1 (MQ=255)                   cAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAAtt                                                                                                                   <  2:126963/143‑1 (MQ=255)                    aGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTg                                                                                                                  >  1:524209/1‑143 (MQ=255)                    aGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTg                                                                                                                  >  2:562498/1‑143 (MQ=255)                     gTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGt                                                                                                                 >  1:872663/1‑143 (MQ=255)                      ttGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCACTTGTa                                                                                                                >  1:373907/1‑143 (MQ=255)                        gTAATCGGCTCATCCAGTGAGCGAGTCGTTGCGAAGAACTAGCCTGATGCTGACCATTTTTCTGCCAGTTCAGTCTCTGTTATTTTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTAtt                                                                                                              <  2:1120418/143‑1 (MQ=255)                           aTAGGCTCACGCTGTGAGCGAGTTGTTGCGGAGAAATAGTTTTATGCTTACCATTTTTCTGGCAGTTTTGTGCCTGTTATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGTGTGTTTTTAATTTGATTGGTCAATTGTATTAAc                                                                                                           <  1:1157848/143‑1 (MQ=255)                              ggCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                                                                                                        <  2:1102783/143‑1 (MQ=255)                                   aTCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTc                                                                                                   <  2:258635/143‑1 (MQ=255)                                       aGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGcc                                                                                               >  1:15425/1‑143 (MQ=255)                                                gttgttGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCt                                                                                      <  1:434744/143‑1 (MQ=255)                                                 ttgttgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAAGCATTGGTCAGCCGATTTTTCTg                                                                                     >  1:897308/1‑143 (MQ=255)                                                  tgttgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGc                                                                                    <  2:221813/143‑1 (MQ=255)                                                    ttgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGa                                                                                  <  2:510807/143‑1 (MQ=255)                                                    ttgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGa                                                                                  >  2:508760/1‑143 (MQ=255)                                                    ttgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGa                                                                                  <  2:540385/143‑1 (MQ=255)                                                    ttgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGa                                                                                  >  2:767774/1‑143 (MQ=255)                                                     tgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGCTCCACTGTCTTAACCATTGGTCAGCAGATTTTTCTGCgtc                                                                                 >  2:227456/1‑141 (MQ=255)                                                       cGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCGCTGGTCAGCTGTTTTTTCTTCTacac                                                                               >  2:396921/1‑143 (MQ=255)                                                       cGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGGTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACAATTGGTCAGCCGATTTTTCTGCGacac                                                                               >  1:62947/1‑143 (MQ=255)                                                        gaagaaATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGTTCAGCCGATTCTTCTGCGACAcc                                                                              >  1:774584/1‑143 (MQ=255)                                                        gaagaaATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACAcc                                                                              >  2:697766/1‑143 (MQ=255)                                                        gaagaaATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAAGAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACAcc                                                                              >  2:68226/1‑143 (MQ=255)                                                        gaagaaATAGCCTGATGCTGACCATATGACGGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTCTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGACTCTTCTGCGACAcc                                                                              >  1:1095953/1‑143 (MQ=255)                                                          agaaATAGCCTTATTCTGACCATTTTTCTGTCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCtt                                                                            <  1:293410/143‑1 (MQ=255)                                                          agaaATAGCCTGATGCTGAGCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCGGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGactgctt                                                                            >  1:915020/1‑138 (MQ=255)                                                          agaaATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCtt                                                                            >  1:59512/1‑143 (MQ=255)                                                            aaaTAGTCTGTTGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTcc                                                                          <  2:301701/143‑1 (MQ=255)                                                             aaTAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCa                                                                         <  1:631113/143‑1 (MQ=255)                                                                 gCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGGGTTTTTAATTTGATTGGTCAATAGTATTCAACGGGGAGCGGCGGATTTGTCTGCGACACCTTCCacaaa                                                                     >  1:257753/1‑139 (MQ=255)                                                                  ccTGATGCTGACCATGTGTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATaa                                                                    <  2:131257/143‑1 (MQ=255)                                                                   cttaTGCTGACCATTTTTCTGCCACTTTTGTCCCTGTAATTCTAAAATAGATTTTGTCTTTAGACTGCGTGTTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTTGTCAGCCGATTTCTCTGCGACACCTTCCAGATaa                                                                    <  1:1016152/140‑1 (MQ=255)                                                                       tGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCAt                                                               >  2:380566/1‑143 (MQ=255)                                                                        gCTGACCGTTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATa                                                              <  1:582223/143‑1 (MQ=255)                                                                         cTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAg                                                             <  2:119067/143‑1 (MQ=255)                                                                               aTTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCAAGATAAGGCATAGCCataa                                                       >  1:288763/1‑143 (MQ=255)                                                                               aTTTTTCTGCCAGATTTGTGCCTGTAATTCTAAAATAGA‑TGTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCACTGGTCAGCCGATTCTTATCCGACACCTTCCAGCTCAGGCATAGcactaa                                                       >  1:1184757/1‑138 (MQ=255)                                                                                 ttttCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCATCTCAGGCATAGCCataata                                                     >  1:930429/1‑143 (MQ=255)                                                                                 ttttCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCataata                                                     <  2:331532/143‑1 (MQ=255)                                                                                 ttttCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCataata                                                     <  2:831042/143‑1 (MQ=255)                                                                                      tGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCGCTCATAGCGGt                                                >  2:324679/1‑143 (MQ=255)                                                                                      tGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGt                                                >  2:371691/1‑143 (MQ=255)                                                                                       gCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTc                                               >  1:994540/1‑143 (MQ=255)                                                                                        ccAGTTTTGTGCCTGTAATTCTAAAATCGA‑GTTGTCTTTAGCCTGAGGGGTGTTTTTAAATTGATTGGTCAATGGTATTAACCATTGGGCAGACGATTTTTCTGAGACACATTACAGATACCGCCAAGCCATAAGGGCGGTcc                                              >  1:581557/1‑143 (MQ=255)                                                                                        ccAGTGTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGGGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTcc                                              >  2:158254/1‑143 (MQ=255)                                                                                         cAGTTTTTTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCa                                             <  1:740124/143‑1 (MQ=255)                                                                                         cAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCa                                             <  2:843532/143‑1 (MQ=255)                                                                                         cAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCa                                             <  2:524209/143‑1 (MQ=255)                                                                                          aGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCAGAGCCGTACTATCGGTCCAg                                            >  1:591517/1‑143 (MQ=255)                                                                                              ttGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCACTTGTCTCAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCAt                                        <  1:1014323/143‑1 (MQ=255)                                                                                               tgtgCCTGTAATGCTAAAAGAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATa                                       <  2:487286/143‑1 (MQ=255)                                                                                               tgggCCTGTAAGTCGAAAAGAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTGTAATTTGATTGGTCAATTGTATTAGCCATTGGTCAGCCCCTTCTTATGCTTCGCCTTCCATATAAGGCATAGCCATAATAGCGGTCCAGTCATa                                       <  2:237671/140‑1 (MQ=255)                                                                                                   ccTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATCATCGCGGTCCAGTCATAGCtt                                   >  2:1057388/1‑143 (MQ=255)                                                                                                    cTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCttt                                  >  2:197972/1‑143 (MQ=255)                                                                                                     tGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCtttt                                 >  2:1211913/1‑143 (MQ=255)                                                                                                       tAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTa                               <  2:872663/143‑1 (MQ=255)                                                                                                        aaTTCTAAATTAAG‑TTCGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAg                              <  2:165937/143‑1 (MQ=255)                                                                                                         aTTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAACTTGATTGGTCAATTGTATTAACAATTGGTCCGCCGATTTTTCTGCGACACCTCCCACATAAGGCATCTCCATAATAGCGGTCCAGTCACACCTATTTatg                             >  1:949601/1‑141 (MQ=255)                                                                                                          ttCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTTCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGa                            <  2:177724/143‑1 (MQ=255)                                                                                                          ttCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGa                            >  1:8616/1‑143 (MQ=255)                                                                                                             tAAAATAGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAAt                         >  2:606242/1‑143 (MQ=255)                                                                                                                  gaGA‑TTTGCCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTACCCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCa                    <  2:1127338/142‑1 (MQ=255)                                                                                                                   aGA‑TTTGTCTTTAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCAGTGGTCAGCCGATTTTTGTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGGCCAGTCATAGCTTGATAGGAAATCAGAAt                   >  1:732810/1‑143 (MQ=255)                                                                                                                          gttttgaGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCt             <  2:101190/137‑1 (MQ=255)                                                                                                                           tgaagatccTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGGCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCtg            >  2:827295/8‑143 (MQ=255)                                                                                                                            cTTTAGCCCTAGGAGTGTTTTTACTTTGATTGGTCAATTGTACTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCtgt           <  2:1223045/143‑1 (MQ=255)                                                                                                                             tttAGCCTGAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCtgtg          <  2:272627/143‑1 (MQ=255)                                                                                                                             tgtggCCTGAGGGGTGTTTTTACTTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCtgtg          <  2:273024/139‑1 (MQ=255)                                                                                                                                     gAGGGGTGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTCTCGGAAATCAGCATAATTCTGTGCAAACCAg  >  2:887333/1‑143 (MQ=255)                                                                                                                                          |                                                                                                                                          ATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGA‑TTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAG  >  NC_000913/1087932‑1088205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑