Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	146.6 / NA	53	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/24);  total (29/24)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA  >  NC_000913/2132650‑2132919                                                                                                                                          |                                                                                                                                     tCTGGTAGAGGGTAAACAGCTGACATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                 <  1:1098290/143‑1 (MQ=255) tCTGGTAGAGGGGAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACATGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                 >  2:1040435/1‑143 (MQ=255)   tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATACGCAGGCTCTGCGt                                                                                                                               <  2:324941/143‑1 (MQ=255)    gggagagGGTGAACAGCTGATATTTTCGGCGGGCGCGGTCGAATTGGCCTTTGTGCTTGCGGGAAAAGTGGATGGACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                              <  2:392463/140‑1 (MQ=255)      tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                            <  2:710625/143‑1 (MQ=255)        gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCCTGATTGATACGCCGGCTCTGCGTCGCct                                                                                                                          >  2:970328/1‑143 (MQ=255)             tAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGGGTCGcttcttat                                                                                                                     >  1:282798/1‑141 (MQ=255)               aaCAGCTGATATTTTTTGCTGGCGCGATCGAATTTGTCTTTGCGCTTGCGGTAAAAGTGGAAGTGCCGGGAGTAGTTTTTCGGTGCCGAGGTGATCTGACTTTCACCGTGATTGATATACAAGCTCTGGGTCGCCtcctctcc                                                                                                                   >  1:606188/1‑141 (MQ=255)                aCAGCTGATATTTTTTGCAGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACAGTGATTGCTATGTAGGCTCTGCGTCGCGTCTTGTAcg                                                                                                                  >  1:136536/1‑142 (MQ=255)                     tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATGTGCGTTTCACGGTGATTGATATGCCGGCTCTGCGTGGACTCTCCTACTTGccg                                                                                                             >  2:842306/1‑142 (MQ=255)                         atTTTTTGCTGGCGCGGTCGAATGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGt                                                                                                         <  1:825149/143‑1 (MQ=255)                          ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATGTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACCGtt                                                                                                        >  1:1232396/1‑143 (MQ=255)                          ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                        >  2:1049194/1‑143 (MQ=255)                          ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                        >  2:1036263/1‑143 (MQ=255)                           tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCGTCTTCTACTTTCCACGGTTc                                                                                                       >  1:1036406/1‑143 (MQ=255)                              ttGCTGGCGCGGTTGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                    <  2:277381/143‑1 (MQ=255)                                gCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCCGTAAAAGTGGAAGTACCCAGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGCTCGccct                                                                                                  >  1:656495/1‑141 (MQ=255)                                       gggTCGCATGTGTCGTTGGGTGTGCGGTAAAGGGGGACGTAGCCGGAGAATGTTTGAGGTGGCGAGGTGGTGTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                           <  1:640341/142‑1 (MQ=255)                                             agttgttCTTTGGGCGTGCGGTAGAAGTGGACGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCTTTCCACGGTTCGCCGTACTCCACCACCAt                                                                                     <  1:52397/137‑1 (MQ=255)                                                      ttttgCTTGCGTTAAAAGCGCAGGTACTCGGAGAATTGTTTCGGTGACGAGGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                            <  1:1105822/140‑1 (MQ=255)                                                           cTTGCGTGAAAAGTTGACGTACCCGGTGCATCTTTTCAGTGAGGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt                                                                       <  1:1209183/143‑1 (MQ=255)                                                            ttGCGGTAAACGTGGACGTCCCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTTGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                      <  1:1117362/143‑1 (MQ=255)                                                                ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGCAAGATGTCGTaa                                                                  >  2:608952/1‑143 (MQ=255)                                                                ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGAAGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTac                                                                  >  2:419241/1‑142 (MQ=255)                                                                  tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATc                                                                >  1:272313/1‑143 (MQ=255)                                                                   aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTGCTCCACCGCCATCCGCAGGAAGCTGTCGtgagtc                                                               >  2:588930/1‑138 (MQ=255)                                                                       gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAGTGCTGGCTTGTGATGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                           <  2:285379/143‑1 (MQ=255)                                                                          gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCACTTCACCGTGATTGATATGCAGGCTCACCGTCGCCTCTTCTCCTCTCCACGGGTCGCCGGACTCGAACACCATCCGCAAGAAGGTGTCGTacaacggtgccc                                                        >  1:157849/1‑132 (MQ=255)                                                                                  cGGAGAATTTTTTCACTGACGAGCTGCTGTGCTGCGCACCGCGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTCTGCCGCTTTCAGt                                                >  1:271049/1‑143 (MQ=255)                                                                                   ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACTACCATCCGCAGGAAGATGTCGTAATCCTGTGCAGCTTTCAGtt                                               >  2:942202/1‑143 (MQ=255)                                                                                    gagaATTTTTTCGGTGAGGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGGGTCACCTCTTGTACTTTCCACGGTTCGCCGTACTCCACCACCGTGCGCCTGCAGATGTCGTAAGCCTGTGGGGCTGTCAGTTc                                              >  2:600059/1‑143 (MQ=255)                                                                                     aggtttttttCGGTGGCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                             <  1:786485/139‑1 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTTCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                        <  2:524311/143‑1 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTTCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTTCCGCTTTCAGTTCGTTATc                                        >  1:94914/1‑143 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                        <  2:573472/143‑1 (MQ=255)                                                                                               cGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGgaca                                   >  2:388779/1‑143 (MQ=255)                                                                                                    gcggggTGATGTGCATTTCACCGTGGTTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATGCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac                              <  1:539822/139‑1 (MQ=255)                                                                                                       aGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGGCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct                           <  2:57709/143‑1 (MQ=255)                                                                                                         gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                         <  2:346900/143‑1 (MQ=255)                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGTCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAGTCCTCTGCCGCTTTCAGTTCGGTAAACAACAAACGctctgcgaa                      >  1:834693/1‑138 (MQ=255)                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                      >  2:637334/1‑143 (MQ=255)                                                                                                             tCTGCATTTCCTCGCGAGTGATCTGCAGGCTCTGCGTCCCCTCTTCTACTTTCCACTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                     <  1:552142/143‑1 (MQ=255)                                                                                                             tCTGCATTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGGTCGGTATCGAACAAACACTCTTTGaaa                     <  1:482381/143‑1 (MQ=255)                                                                                                              cttctgttCACCGAGATTACTATGCATGCTCTGCGTCTCCTCTCCTACTTTCGACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCTTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc                    <  1:900609/137‑1 (MQ=255)                                                                                                               tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCCGGTCGGTACCGAACAAACAGTCCTTgcacca                   >  1:33500/1‑138 (MQ=255)                                                                                                                gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCCTCTTTGAAAccc                  >  2:213431/1‑141 (MQ=255)                                                                                                                       aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGCTCGGTATCGAACCAACACTCTTTGAAACACCATGccc           >  2:5991/1‑143 (MQ=255)                                                                                                                        ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa          <  1:198922/143‑1 (MQ=255)                                                                                                                         cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg         >  1:250549/1‑143 (MQ=255)                                                                                                                          gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAgg        >  2:721976/1‑143 (MQ=255)                                                                                                                              ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACCCTTTGAAACGCCATGCCCAGGTaaa    >  1:163432/1‑143 (MQ=255)                                                                                                                               tgatAGGCAGGCTCTGCTTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg   <  1:787765/143‑1 (MQ=255)                                                                                                                                gatATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa  <  2:735089/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA  >  NC_000913/2132650‑2132919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑