Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	12,435	C→A	9.7%	F91L (TTC→TTA)	dnaK →	chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,435	0	C	A	9.7%	273.8 / 5.1	91	F91L (TTC→TTA)	dnaK	chaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base C (64/16);  new base A (2/9);  total (66/25)
Fisher's exact test for biased strand distribution p-value = 9.72e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACG‑ATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAA  >  NC_000913/12197‑12671                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              ccccccttttttgtgCGTTTTTGTATGTCCCCACTCCTCGGGTTCTGGGTGAGCCCGTAGGCTATCCGCCCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑TGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑c                                                                                                                                                                                                                                               <  1:333748/225‑1 (MQ=255)    aCTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGACACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACCAAGAAGGAAAGCGTGAGGTTTCCCTCATCCCCTT‑Caaa                                                                                                                                                                                                                                            >  1:614275/1‑239 (MQ=255)    aCTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGCCGAGGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑Caaa                                                                                                                                                                                                                                            >  2:231508/1‑239 (MQ=255)    aCTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑Caaa                                                                                                                                                                                                                                            >  2:767340/1‑239 (MQ=255)    aCTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑Caaa                                                                                                                                                                                                                                            >  2:408867/1‑239 (MQ=255)    aCTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑Caaa                                                                                                                                                                                                                                            >  2:428218/1‑239 (MQ=255)    aCTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑Caaa                                                                                                                                                                                                                                            >  2:611808/1‑239 (MQ=255)    aCTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGGCAGCCGGCTAAACGTCAGGCAGTGACGAAACCCCAAAACACTC‑TG‑CTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCAAGGACAAAGAGGAACAGCGCGATGTTTCCATCTCTCCGTT‑aaaa                                                                                                                                                                                                                                            >  1:874772/1‑239 (MQ=255)         tgtgTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCCCTTCCAGGACGAAGAAGTACACCGGGAGGTTTCAATAATGCCGTT‑AAAAaatat                                                                                                                                                                                                                                       >  1:237631/1‑239 (MQ=255)           tgtAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAattatt                                                                                                                                                                                                                                      >  1:453021/1‑238 (MQ=255)           tgtAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCAATCATGCCGTT‑CAAAattatt                                                                                                                                                                                                                                      >  2:177932/1‑238 (MQ=255)           tgtAGCCATTAAGGATGGAACCATCCCTCGCGTGCTGGAGAACGCCGAAGGGGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATtg                                                                                                                                                                                                                                     <  2:308394/239‑1 (MQ=255)            gtAGCGATTTAGGATGGCCCCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATtg                                                                                                                                                                                                                                     <  2:237631/238‑1 (MQ=255)                cGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAACCTC‑TAGTTGGTCAGCCGGCTAAACGTCCGGCAGTGACGCACCCGCAAAACCCTC‑TG‑TTTG‑CGATTCAACGCCTGATTGGTCTCCGCTTCCAGGACGAAAAAGAACAGCGGGATTTTTCCCTAATGCCGTT‑CAAAATTATtgcgcct                                                                                                                                                                                                                                >  1:634457/1‑235 (MQ=255)                cGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCCCCGCTTCCAGGACAAAAAAGGAAAGCGGGAGGTTTCCACAATGCCGTTACaaaactgtctttta                                                                                                                                                                                                                                 >  1:662220/1‑229 (MQ=255)                          tggCACCCCTCCCCGCGTGCTGGGGAACGCGGAAGGCGATCGCACCCCGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGGCAGCCGGCTAACCGTCAGGCAGTGACGAACCCGCAAAAACCTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGc                                                                                                                                                                                                                       <  1:702042/238‑1 (MQ=255)                             caccacTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCACGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGAc                                                                                                                                                                                                                    >  2:732892/1‑238 (MQ=255)                               ccacTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCa                                                                                                                                                                                                                 <  2:614275/239‑1 (MQ=255)                                  ctcctcGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATAACCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGAAGCGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCCCCGCTTCCAGGCCGAAGAAGGACAGCGTGATGTTTCACTCATGACGTT‑CCAAATTATTTCTACAGATCACGGCGagggctgg                                                                                                                                                                                                              >  1:741560/1‑232 (MQ=255)                                    cctcGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGt                                                                                                                                                                                                            <  2:453021/239‑1 (MQ=255)                                      cggggggCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTTTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGACAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCg                                                                                                                                                                                                          <  2:634457/233‑1 (MQ=255)                                       cgcgTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGa                                                                                                                                                                                                         >  2:680275/1‑239 (MQ=255)                                             cTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTaa                                                                                                                                                                                                   >  2:588594/1‑239 (MQ=255)                                                   tccGCCGAGGGGGTGCGCACCACCCGTTTTATCATTGCCTATCCCCCGGCTAGTGAACCTA‑TAGTGTGTCAGCAGGCTAAACGTCAGGTAGTGACGAACCCCCAAAACACTC‑GT‑TTTC‑AGATCAAACGCCTTATTGGTCGCCGCTTCTAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTCTTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCa                                                                                                                                                                                             <  1:566610/237‑1 (MQ=255)                                                    aCGCCGAAGGCGATCGCACCACGCCTTCTATCATGGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGGCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCa                                                                                                                                                                                             <  2:379897/238‑1 (MQ=255)                                                     cGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGa                                                                                                                                                                                           >  2:74475/1‑239 (MQ=255)                                                        cGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCAt                                                                                                                                                                                                                <  2:717474/215‑1 (MQ=255)                                                        cGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCAt                                                                                                                                                                                                                >  1:717474/1‑215 (MQ=255)                                                        cGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGaaaa                                                                                                                                                                                        >  2:576038/1‑239 (MQ=255)                                                        cGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGaaaa                                                                                                                                                                                        >  2:368070/1‑239 (MQ=255)                                                          aaGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGCTGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAACCGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGGGATGTTTCCATAATGCCGTT‑CAAAATTTTTCCTGTTGTAAACGGGGCCGCATGGGGCGAAGTTAAAGGCCaaaaaaag                                                                                                                                                                                      >  1:847151/1‑237 (MQ=255)                                                          aaGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGaaaagg                                                                                                                                                                                      >  1:609872/1‑237 (MQ=255)                                                          aaGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATg                                                                                                                                                                                      >  1:586708/1‑239 (MQ=255)                                                                           ccTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTT‑AAGGCCAAAAAAAGGACccgccgacaatt                                                                                                                                                                       >  1:334093/1‑230 (MQ=255)                                                                               cTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGGCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTct                                                                                                                                                                    >  2:396051/1‑236 (MQ=255)                                                                                  ttccttccTTTACCCGGGGGGGGGAAACTCTTTGGTGGGCCGCCGGCAAAGCGTCGGGGGGGGACCACCCCGCAAACCCCGT‑TG‑GTTG‑CGATTAACCCCCTGATTGGTCGCCGTTTCAGGGAAAAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑AAAAATTATTGCTGCTGATACCGGCGACGCAGGGGTCGAAGTAAAAGCCAAGAAAAGGGCACCGCCGCAGATTTCTGCTGa                                                                                                                                                               <  1:612550/233‑1 (MQ=255)                                                                                  tCATTTCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGt                                                                                                                                                                                                            <  2:893065/193‑1 (MQ=255)                                                                                     ttGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGAACCGCCGCAGATTTCTGCTGAGGTg                                                                                                                                                           >  2:718225/1‑239 (MQ=255)                                                                                          tataCCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGGTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGCCGCCAGGGCCGAGGTTAAAGGCCAGAAAATGGCACCGCCGACGATTTCTGCCTAAGTGTTGaa                                                                                                                                                      >  2:217278/1‑239 (MQ=255)                                                                                            taCCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGCCGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGaaaa                                                                                                                                                    >  2:862160/1‑239 (MQ=255)                                                                                            taCCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGGGCTGaaaa                                                                                                                                                    >  2:365583/1‑239 (MQ=255)                                                                                                cAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAAt                                                                                                                                                >  2:142528/1‑239 (MQ=255)                                                                                                  ggATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGTTAACGGCGACGAATGGGTCGAAGTTAAAGGCCAGAAAATGGCACGTCCGCGGATTTCTGCGGAAGGGCTGAAAAAAATga                                                                                                                                              >  2:468946/1‑239 (MQ=255)                                                                                                  ggATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGGCGAAGGGCTGaaaaaaaaaa                                                                                                                                              >  1:257479/1‑236 (MQ=255)                                                                                                   gATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATTCCGTT‑CAAAATTATTGCTGCTGATAACGGCGAAGCATGGGTCAAAGTTAAAGGCCAGAAAATGCAACCGCCCCAGATTTCTGCGGAAGGGCGGaaaaaaaaaaa                                                                                                                                             >  1:147249/1‑235 (MQ=255)                                                                                                   gATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGACGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGGCGAAGTTAAAGGCCAACAAATGGCACCGCCGCAGCATTTTATTGAAGTGCGGaaaaaaaaaaa                                                                                                                                             >  1:889269/1‑235 (MQ=255)                                                                                                        tGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGaaa                                                                                                                                         >  2:492989/1‑238 (MQ=255)                                                                                                        tGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGGGCTGAAAAAAATGAAGaaa                                                                                                                                         >  2:605087/1‑238 (MQ=255)                                                                                                        tGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTACTGTGGAGGTGCTGAACAACATCAAGaaa                                                                                                                                         >  2:608531/1‑238 (MQ=255)                                                                                                        tGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGAGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑AGAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGTAAAAACTGaaaaaa                                                                                                                                         >  2:565227/1‑238 (MQ=255)                                                                                                        tGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGCCAATGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCATCCCAGACGAAGAAGTACAGCGTGAAGTTTACATCCTGCCGTT‑CAAAATTATTACTGCTGATAAGGGCGACGCATGGTGGGAAGTTAAAGGCCAACAAAGGGCACCGCCCGAGATTTGTGCTGAAGGGATGAAAAAAAGgaaaaca                                                                                                                                         >  1:773749/1‑234 (MQ=255)                                                                                                            aCTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGGGCTGAAAAAAATGAAGAAAAcc                                                                                                                                      >  2:791756/1‑237 (MQ=255)                                                                                                             ctc‑tAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCt                                                                                                                                   >  2:297376/1‑239 (MQ=255)                                                                                                             ctc‑tAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGACTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGCCGAATTTAAAGGCCAGAAAATGGCCCCGCCGCAGATTTCTCATGCAGGGCTGAAAAAAAGaaaaaaaaag                                                                                                                                      >  1:79420/1‑234 (MQ=255)                                                                                                              tc‑tAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATACCGGCGACGCATGGGCCGAAGTTAAAGGCCAGAAAATGGCCCCCCCCCAGATTTCTGCTGAAGGGCTGAAAAAAATTAAAAAAAcccccg                                                                                                                                  >  1:118832/1‑235 (MQ=255)                                                                                                                  ggtttggccgcgcgCTAACCGTGAGGCAGTCACCAACCCGAAACACCCGT‑TG‑TTGG‑TGATTAAGCGCCTGATGGGGCGCTGCTTCGAGGACAAAAAAGTACAGGGGGTTGTTTCCAGACTGCCTGT‑AAAAATTATTGGTGCTGAAACGGGCGAGACTGGGGTCGAAGTAAAGGGCAAAAAAATGGCCCCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAg                                                                                                                               <  1:767340/226‑1 (MQ=255)                                                                                                                  aGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATtg                                                                                                                                                                                                                                     <  1:482081/137‑1 (MQ=255)                                                                                                                  aGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATtg                                                                                                                                                                                                                                     >  2:482081/1‑137 (MQ=255)                                                                                                                  aGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAAAGCCTGATTGGTCGCCGCCTCCAGGACGAAGAAGTCCAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTACTGCTGCTGATACCGGCGAAGCATGGGTCGAAGTTAAAGCCAGGAAAATGGAACAGCCGAGGATTTCTGCTGAAGGGCTGCAAAAAATAAAAAAAACCGCAGaaa                                                                                                                               >  1:224017/1‑238 (MQ=255)                                                                                                                       gTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGTCACCCCCGCAGATTTTTGCTGAAGGGCTGAAAAAAATGAAAAAAACCGCGGAAGAtaa                                                                                                                           >  1:394879/1‑236 (MQ=255)                                                                                                                         ccgccggcaacagctCAGGCGGTCACAACCCCGCAAAACCCTG‑TT‑TTTG‑CTATTAAACCCTTGTTTGGCCGCCTCTTCCGGGAAGAAGAAGGACAGGGTGCTTTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATACCGGAGACGGATGGGTCAAAGTTAAAGGCAAAAAAATGACACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCt                                                                                                                        <  1:718225/225‑1 (MQ=255)                                                                                                                         cAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTACAGGCCCGAAAATGGCACCGCCGCAGATTTCTGCTGAAATGCTGAAAAAAATGAAGAAAACAGCTGAAGATTa                                                                                                                           >  2:491243/1‑236 (MQ=255)                                                                                                                             ggttaaaacggagagaggggccGACCCCCCAAAACCATT‑TT‑TTTG‑CGATTAAGCCTGTGATGGGTCGCCTATTCCAAGAAAAAGAATAACGGGTTGATTTTCCAAATATGCCGTT‑AAAAATTATTGCGGCTGATAACGGCGACTCATGGGGCGAAGTAAAAGACAAAAAAAGGGAACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGt                                                                                                                    <  1:680275/218‑1 (MQ=255)                                                                                                                                          ggCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTg                                                                                                       >  2:274662/1‑239 (MQ=255)                                                                                                                                            gtggagacgaCCCCCAAAACCCTT‑TGTTTTG‑CATTTAACCGCTTGGTTGGCCGCCGTTTACAGGAAGAAAAGGTAGAGGGTGATTTTCCCATCATGCCGTT‑AAAAATTATGGCTGCTGATACGGGCGCCGAATGGGTCAAAGTTAAAGGCAAAAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTg                                                                                                       <  1:365583/229‑1 (MQ=255)                                                                                                                                             agggACGAACCCG‑AAAACCCTCTTG‑TTTG‑AGATTAAACGCTTGATGGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCAGGCCGTT‑AAAAATTATTGCTGCTGATAACGGCGACGAATGGGTCGAAGTTAAAGGCAAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGaa                                                                                                     <  2:387799/235‑1 (MQ=255)                                                                                                                                              gtacccaaCCCGAAAACCACTT‑GT‑TTTG‑CGATTAACCGCCTGATTGGTCGCCGTTTCCAGGAAGAAGAAGTCCAGCGTGATGTTCCCATCATGCCGTA‑CAAAATTATTGCTGCTGATAACGGCGACGAATGGGTCGAAGTTAAAGGCCAAAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAg                                                                                                    <  2:609872/232‑1 (MQ=255)                                                                                                                                               tGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAACTTATTGCTGCTGATAACGCCGACGCATGGGTCGAAGTTAAAGGCTGGAAAATGGCCCAGCCCCAGATTTCTGCTGAAGTGCTGCAAAAAATAAAAAAAACCCCTTAAATTAACCTGGGGGAACCGTAAAATGaa                                                                                                     >  1:227969/1‑236 (MQ=255)                                                                                                                                                         gCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATACCGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGGGAACCGGTAAATGAAGCGGTTATAACCg                                                                                        >  1:758347/1‑239 (MQ=255)                                                                                                                                                          cAAAACCCTG‑TT‑TTTGTCAATTAAACCTCTGTTTGCTCCCCTCTTCCGGGACGAAGAAGTACAGCGTGATGTTCCCATACGGCCTTT‑CAAAATTATTGCTGCTGATAGCGGCGACGCATGGGGCGAAGTTAAAGGCAAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCg                                                                                        <  1:588594/239‑1 (MQ=255)                                                                                                                                                            aaacccctttG‑TTGG‑CAATTAACCCCCGGATTGGCCGCCGCTCCAAGGACGAAGAAGTACAGGGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCGGCTGATAACGGCGACGCATGGGTCGAAGTTAAGGGCAAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTa                                                                                      <  2:586708/230‑1 (MQ=255)                                                                                                                                                                 ctc‑tG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCgcc                                                                                 >  2:451725/1‑236 (MQ=255)                                                                                                                                                                  tc‑tG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCTCTGAAAATTACCTGGGGGAACCGGTAACTGAAGCGGTTATCACAGAACCggaaa                                                                               >  1:523459/1‑236 (MQ=255)                                                                                                                                                                   tcttttttG‑CGATTAACCGCCTGATTGGGCGCCGCTCCCAGGACGAAGAAGTACAGCGTGAGTTTCCCATAATGCCGTT‑CAAAATTATTGCGGATGATAACGGCGAGGAATGGGTCGAAGTTAAGGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa                                                                                <  2:230816/234‑1 (MQ=255)                                                                                                                                                                    tcttgtttgcgataaatgcgcgtaTTGGCCGCGGCTACAAGGAAGAAGAATTCCGGCGGAATGTTTCCTTAAGGCATTT‑AAAAATAATTGCGGCTGAAAAGGGCAACGGATGGGTGGAAGATAAAGGCAAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa                                                                                <  2:523459/216‑1 (MQ=255)                                                                                                                                                                    ccttgttgtcctttaaaccctgtaTTGCTCGCCGTTTCCAGAACGGAAAGGTACAGCGTGATGTTTCCATCATGCCTTT‑AAAAATTAGTGCTGCAGAGAACGGCGACGCGTGGGGCGAAGTTAAGGGCAAAAAAAGGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa                                                                                <  1:420134/216‑1 (MQ=255)                                                                                                                                                                      g‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACt                                                                            >  1:677389/1‑239 (MQ=255)                                                                                                                                                                         tttgcattaaaaccctggtttGGTCGCCGCTCCCGGGAAGAAGAAGTAAAGCGTTATTTTCCCAACAGGCCGTA‑AAAAATTTTTGCGGCTGAAACCGGCGACGAAGGGGGCAAAGTAAAGGGCAAAAAAAGGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACtt                                                                           <  2:257479/220‑1 (MQ=255)                                                                                                                                                                               aTTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACG‑a                                                                    >  2:678578/1‑239 (MQ=255)                                                                                                                                                                                   aaCGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACG‑ATGc                                                                 >  2:580124/1‑238 (MQ=255)                                                                                                                                                                                   aaCGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGGGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATAACCGTACCGGCATACTTTAACG‑ATGc                                                                 >  2:778820/1‑238 (MQ=255)                                                                                                                                                                                   aaCGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGAACCGCCGCAGATTTCTGCTGAAGCGCTGATAAAAATGAAGAAAACCGCTGAAGATTCCCTGGGTGAACCGGTAAAGAAAGCTGTTATCACCAAACCGGCAACATTTAAGG‑ATGcc                                                                >  1:430280/1‑238 (MQ=255)                                                                                                                                                                                                         ccAGGAAAAAAAAGTACAGCGTAGTTTTTCCATCATGCTTTT‑AAAAATTTGTGTGTGTGAAACCGGCGACGTATGGGTCGAAGTAAAAGGCAAGAAATGGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACG‑ATGCTCAGCGTCAGGCAACCAAAGACg                                          <  2:428175/239‑1 (MQ=255)                                                                                                                                                                                                                  agaagTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGCAACCGCCGCAGATTTCTGCTTAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGCTTACCTGGGGGAACCGGTAACTGAAGCGGTTTTCACCGTACTGGCATACTTTAACGAATGC‑CAGCACAAGGCAACCaaaaagccagaccata                                 >  1:783905/1‑226 (MQ=255)                                                                                                                                                                                                                       tACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCAAACTTTAACG‑ATGCTCAGCGCCAGGCAACAAAAAACCAAGGcggaaacgcg                            >  1:398704/1‑230 (MQ=255)                                                                                                                                                                                                                         cAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGGGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACG‑ATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTgg                          >  2:648408/1‑239 (MQ=255)                                                                                                                                                                                                                            cGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGGGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGGGAACCGGTAACTGAAGCTGTTATCACCGAACCGGCATAATTTACCG‑ATGTTCAACGCCAGGCAACAAAAAGAGCAGGcgggctcagcggcag                       >  1:713165/1‑225 (MQ=255)                                                                                                                                                                                                                              tGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTTGAAGTTAAAGGACAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATCCTTTAACG‑ATGCTCAGAGTCAGGCAACCAAATACGCAGGCCGTATGACTggcttga                     >  2:89513/1‑234 (MQ=255)                                                                                                                                                                                                                                 tGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAGGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACATGGGTGAACCGGTAAATGAAGCTGTTATCCACGTACCTGCATAATTTAACG‑ATGCTCAGGGTCAGGCAACAAAAACACCAGGCCGTATCCCAGGCCTGGaat                  >  1:61631/1‑238 (MQ=255)                                                                                                                                                                                                                                 tGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCAc                                                                                          >  2:665222/1‑168 (MQ=255)                                                                                                                                                                                                                                             gCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGAACCGGCATACTTTAACG‑ATGCTCACCGTCAGGCAACCACAGACGCGGGCCGTTCCGCTGGTCTGGAAGAAAAACgaaaaa      >  1:341408/1‑234 (MQ=255)                                                                                                                                                                                                                                             gCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCGGTTATCACCGTACCGGCATACTTTACCG‑ATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGGATCGCTGGTCTGGAAGTAAAACGAatca      >  2:564992/1‑239 (MQ=255)                                                                                                                                                                                                                                                 tt‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTACCG‑AGGCTCATCGTCAGGCAACCAAAGCAGCAGGCCGTATCGCTGGTCTGGAAGGAAAACGAACCATaaa  >  1:764820/1‑239 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              CCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTC‑TAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTC‑TG‑TTTG‑CGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTT‑CAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACG‑ATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAA  >  NC_000913/12197‑12671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑