Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 159,258 G→T 20.5% V285V (GTC→GTA gluQ ← glutamyl‑Q tRNA(Asp) synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913159,2580GT20.5% 85.3 / 5.8 61V285V (GTC→GTAgluQglutamyl‑Q tRNA(Asp) synthetase
Reads supporting (aligned to +/- strand):  ref base G (19/28);  new base T (11/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGT  >  NC_000913/159032‑159495
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
aTCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCCAGTTTTTAAGGcggactga                                                                                                                                                                                                                                         >  2:173883/1‑239 (MQ=255)
       tCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTCAGCCGCCCGGTTTTTACGGGTGAACGAAgagttt                                                                                                                                                                                                                                  >  1:129313/1‑234 (MQ=255)
         aCCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTTCCGACTCGGGCACGGCGGTAAGCCGCCCGTTTTTGACGGCTTGCTGAAGGATTTGc                                                                                                                                                                                                                                >  2:374885/1‑239 (MQ=255)
             gtgGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAACCGCCAGTTTTTTAAGGCTCACCTAAGGGTTTtctcc                                                                                                                                                                                                                             >  2:291216/1‑237 (MQ=255)
               ggACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGGAAACCCCCCGGTTTTGTCGGCTGGCCAAAGGATTTgtcccggc                                                                                                                                                                                                                          >  1:505020/1‑232 (MQ=255)
                           gcttttttaGCCTCTTTTTCCCTGCGTCGTAGGCCCTTGCGGAAAAAATTAGGCACTGGGGTAAAAATAGTACACCTCGGTAGTGTAAAACCTCATTCAGGACAAAAAAATAGCGGCCAATCATAGCTCCGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGc                                                                                                                                                                                                            <  1:137578/231‑1 (MQ=255)
                                                   cgGTTTAGCCCCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACCCCTCGGTAGTGTCAAACATCATTCAGGAAAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGcccc                                                                                                                                                                                   <  1:291216/239‑1 (MQ=255)
                                                     gCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGCCGGTAAGCCGCCCGTTTTTTACGGCTCACTGAAGGGTTTTCTCGCCCCGTAAATCCTGCCAAGTGGCTTCTTCCGCCTGCCCCAg                                                                                                                                                                                 >  2:507532/1‑239 (MQ=255)
                                                      tttgggcccTTGGGGAAAAAATTGGCGACTCGGGTAAAAATAGTCCCCCCCGGTAGTTGCAAAAATGATTCAGGACAAAAAAATAGCGTGTAATCATAGCCCAGCATGACGCATTTGAGAAATTTGAATTTAAAATTGCCGACTCGGGCACGGAGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGa                                                                                                                                                                                <  1:700223/232‑1 (MQ=255)
                                                        taaccccTTGCGGCAAAAATTAGCGAATCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGaaa                                                                                                                                                                              <  2:129313/234‑1 (MQ=255)
                                                                tGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAACCGCCAGGTTTTTACGGCTGACTTAAGGGTTTTGCCCAGGCCGAAAACCCGCCAGGGGGGTTTTGCCTCCTGCCCCCGaaaattaaatg                                                                                                                                                                      >  1:181107/1‑231 (MQ=255)
                                                                     aaaaatTTAGGCACCCGGCTAAAAATGGTACCCCAGGGTGGGTGTCAACCTCTTGCAGGAAAAAAAAATGGCGGCTAAGCAGAGCTCGGAATGACTCTTTTGAGAATGTGGAATTGACAATTGCAGACTCGGGCACGGTGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGAGGCTGAAATCCTGCCAGTGGGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGcc                                                                                                                                                                   <  1:642018/235‑1 (MQ=255)
                                                                             aGCCACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCGAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTAc                                                                                                                                                       <  2:732943/239‑1 (MQ=255)
                                                                                         aaaaaTAGTACACCGCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGAt                                                                                                                                            <  2:181107/238‑1 (MQ=255)
                                                                                          aaaaaaGTACCCCTCGGGTAGGTCAAAACGTAATCAGGACAAAAAAATAGCGGGTAAGCAAAGCTCAGGATTGAGCATTTGAGAATTGTGAATTTACAATTGCCGACTCGGGCACGGTGGTAAGCCGACAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCg                                                                                                                                          <  2:457678/239‑1 (MQ=255)
                                                                                             aTAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGGAAACCGCCAGTTTTTGTCGGCCGCCGCAAGGAATTTGTCGGCGCTGAAAACCTTCCCATGGGTCTTTGCCTGTGGCCCCCGAAATAGAATGACGCCGATTATAACCGGGGGGGGagagc                                                                                                                                         >  1:722752/1‑233 (MQ=255)
                                                                                                 tACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTTAACCCGCCCGTTTTTTAGGCTGACCTAAGGCTTTGCTCGGCGATGTAATCCCTGCCATGGGCTTTCCCCTCCTGCCCCCCAAATTTAAATGCCCGCGTTGGAACCGGGCGTgggaccccattg                                                                                                                                    >  1:382724/1‑228 (MQ=255)
                                                                                                 tACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCCGAAATTGAAGTGCCGCGATTTGTACCTGGCGTGGATCCCCttttg                                                                                                                                   >  2:882130/1‑237 (MQ=255)
                                                                                                 tACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGGTTTTTACAGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAAGTGGCTTCTGCCTGCGGCCCCAGCAATTTGAGTGCCGCGATTAGGACCGGGGCTGGTACCCCTTTCg                                                                                                                                   >  2:390476/1‑239 (MQ=255)
                                                                                                  accccTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCAGAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCgg                                                                                                                                  <  2:828460/236‑1 (MQ=255)
                                                                                                      cTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGGTTTTTACGGCTGACTGAAGGGTTTTCTCGACGCTGGAATCCTGCCAGTGTGCTTCTTCCTGCTGCCCCAGAAATTGAAAGGCCGCGCTTTATAACCGGCGGGAGTCGCCTTTCGGCaa                                                                                                                               >  2:345646/1‑238 (MQ=255)
                                                                                                      cTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGGTTTTGACGGCTGACTGAAGGAGTTGTCCGACGCTGGAAACCTTCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTTAAGTGCCGCGGTTAGTACCGGGCGTGGATCCCCTTTCGGcca                                                                                                                               >  2:401375/1‑236 (MQ=255)
                                                                                                        cGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTTACCGCTGACTTAAGGATTTGCTCGCCGCTTAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCCGAAATTTAAGTGCCGCGATTACAACCGGGCGTGGACTGCCATTCGGCacagc                                                                                                                            >  1:190364/1‑235 (MQ=255)
                                                                                                         tggTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGc                                                                                                                            <  2:473027/238‑1 (MQ=255)
                                                                                                            tAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCCGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGGTCGCCTTTCGGCAACGCAg                                                                                                                          >  2:199665/1‑238 (MQ=255)
                                                                                                            tAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTTGAGTGCCGCGATTATTACCGGGCGTGGATCCCCTTTCGGCCACGCAgg                                                                                                                         >  2:4679/1‑239 (MQ=255)
                                                                                                                tgtCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCCCGACGCTGAAATCCTTCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGGGCCGCGGTTAGGACCGGGCCTGGATCCCCTTTTGGCAACGCAGgggc                                                                                                                      >  2:846874/1‑239 (MQ=255)
                                                                                                                tgtCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAACCGCCCATTTTTGACGGCTGATCGAAGGGTTTGCTCGCACCCGAAACCCTGCCCGGGGGCTTTTCCCTGCTTCCCCCGAAAATAAAAGGCCGCGGTTAGTAACGGGCGGGGAGTCCCTTTCGGGaaaacaagggg                                                                                                                      >  1:235419/1‑230 (MQ=255)
                                                                                                                          catTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAAGGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGGGCATGGTTCt                                                                                                              >  2:547653/1‑237 (MQ=255)
                                                                                                                            ttCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCCACGCAGGCGCATGATTTTGCtt                                                                                                          >  2:708118/1‑239 (MQ=255)
                                                                                                                               aGGAAAAAAAAAATGCGGATAATCACAGCCCAGCATGACTCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGa                                                                                                       <  1:547653/239‑1 (MQ=255)
                                                                                                                                 gACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGaaa                                                                                                     >  2:167141/1‑239 (MQ=255)
                                                                                                                                 gACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAAGGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCCACGCAGGGGCATGATTCTGCTTGGaaa                                                                                                     >  2:710151/1‑239 (MQ=255)
                                                                                                                                      aaaaaTAGCGGCTAATCATAGCTCGGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGcc                                                                                                                                                                   <  2:197616/173‑1 (MQ=255)
                                                                                                                                      aaaaaGCGCGTCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTa                                                                                                <  2:668638/239‑1 (MQ=255)
                                                                                                                                       aaaaTAGCGGCTAAGAAAAGACCAGCATGCCGCATTTAAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAg                                                                                               <  2:427961/239‑1 (MQ=255)
                                                                                                                                        aaatagggtcatatcattcgtCAGCATGCCTCATTTGAGAATTTTGAATTTACAATTGCCGCCTCGGGCACGGCGGTAGGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAACTCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAgc                                                                                              <  1:649056/219‑1 (MQ=255)
                                                                                                                                        aaaTGGCGGTTAAACATAGCCCAGCAGGCAGCATTTGATAATGTTTAATTTCCAATGGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACGGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAgc                                                                                              <  1:456173/239‑1 (MQ=255)
                                                                                                                                              cGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTTACGGCTGACTGAAGGATTTGCTCGACGCTGGAATCCTGCCAGTGTGCTTCTGCCTGCGGCCCCCGAAATTGAAGTGCCGCGGTTATTACCGGCCGTGGGTCGCCTTTCGGCAACGACGCGGCAGGATTCTCGCTGGAAAGATTAACGCCttt                                                                                        >  1:98434/1‑238 (MQ=255)
                                                                                                                                                     ccaaagccccggaTGACGCTTTTGGGATTTTTAAATTTACATGTGCCGGCCCGGGCACGGCGGTAAGCCGCCTGTTTTTGACGGCTGACTGAAGGATTTGCCCGACGCTGAAATCCTGCCAGTGGGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTa                                                                                 <  1:852710/227‑1 (MQ=255)
                                                                                                                                                               gCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTTTGGATTAAGGGCCAACg                                                                       >  2:168813/1‑239 (MQ=255)
                                                                                                                                                                gtggACGTATTTGAAAATTGTGAATTTACAATTGCCGGCTGGGGTACGGAGGTAAGCCGCCGGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTTAAATCCTTCCAGTGTGCTACTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCgg                                                                      <  1:184561/236‑1 (MQ=255)
                                                                                                                                                                cATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAACGTTTAGCGCCTTTTGGGTTAAAGGCCAGCgg                                                                      >  2:399549/1‑239 (MQ=255)
                                                                                                                                                                cATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGGACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGATTATCGCCTTTTGGATTAAGCGCCAGCgg                                                                      >  2:810868/1‑239 (MQ=255)
                                                                                                                                                                 atggcggttttGAGAATTTTGAATTTACAAATGCCGTCTCGGGCCCGGCGGTAAGCCGCCAGTTTTGGCCTGTTGATGGAAGGATTTGCTCGAAGCGGAAATCCTGCCAGTGTGCGTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCgg                                                                      <  1:173883/230‑1 (MQ=255)
                                                                                                                                                                     cGCATTTGAGAATGTTGAATTTCCAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGa                                                                  <  2:118418/238‑1 (MQ=255)
                                                                                                                                                                          ttGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGGAACGCAGGCGCATGATTCTGCTTGGAAAGTTTTGCGCCTTGGGGATTAAGGGCCAGCGGCAGGTGAATg                                                            >  2:489655/1‑239 (MQ=255)
                                                                                                                                                                              gaATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCCGGCGGGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGTTTAAGCGCCAGCGGCAGATGAATGTaa                                                         >  2:851717/1‑238 (MQ=255)
                                                                                                                                                                                    tGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGGGTGCTTCTGCCTGCTGCCCCAGAAATTGTAGGGCCGCGATTAGTACCGGGCGTGGGTCGCCTTTTGGGAACGCCAGGGCATGATTCTGCTTGGAAAGGTTAGCGCCCTGTGGGTTAAGCGCCAGTGGCaacaggaaggaatttggg                                                   >  1:306900/1‑221 (MQ=255)
                                                                                                                                                                                           aCAATTGCCGACTCGGGCACGGCGGTAAGCCGACAGATTTTTACGGCTGGCTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCCGAAATTGAAGTGACGCGATTAGTACCGGGCGTGGATCGCCTTTCCGCAACGCCGGAGCATGGTTCTGCCTGGAAAGTTTAGCGCCTTGTGGTATAAGCGCCACCGGCAGATACATGTAAACTGGGACTTTCCa                                          >  1:169156/1‑239 (MQ=255)
                                                                                                                                                                                                tGCCGACTCGGGCGCGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCaa                                     <  2:190364/239‑1 (MQ=255)
                                                                                                                                                                                                             aCGGGGGTAAGCCGCGAGTTTTTGGAGGGTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAg                        <  1:199665/239‑1 (MQ=255)
                                                                                                                                                                                                              cgggggTAAGCCCGCAGTTTTGGACGGGTGACTTAAGGATTTGGTCGAAGCTGAAATCCTGGCGAGTTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAg                        <  1:374885/238‑1 (MQ=255)
                                                                                                                                                                                                               ggcggTAAGCCGCCAGGTTTTTACGGCTGACTGAAGGGTTTGCTCGACCCTGAAATCCTGCCAGGGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGGGGATCGCCTTTCGGCAAAGCAGGCGCATGATTCTGCTTGGACAGTTTAGCGCCTTGTGGGTTAAGAGCCAGCGGCCGATGCATGTAATCTGGCCCTTTCCCCGCCaaaaaacgggaaaacg                       >  1:33688/1‑228 (MQ=255)
                                                                                                                                                                                                                        gccgccATTTTTGGGCGGCTGTGTGAAGGTTTGGCTGCACGCTGAAATCCTGCCAGTGTGCTTCTGGCTGGTGCCCCAGAAATGTAAGTGCCGCGATTAGGACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTg               <  1:345646/238‑1 (MQ=255)
                                                                                                                                                                                                                         ccgccAGTTTTTTACGTCTGACTGAAGTATTTGCCCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGcc             <  1:168813/239‑1 (MQ=255)
                                                                                                                                                                                                                           cccccgTTTTTTGCGGTTTACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCt            <  1:791552/234‑1 (MQ=255)
                                                                                                                                                                                                                           ccccAGTTTTTGGCGGCTGACTGAAGGATTTTCCCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCt            <  1:882130/237‑1 (MQ=255)
                                                                                                                                                                                                                            cccATTTTTTGCCGGTTGACTGAAGGTTTTGCTCGACGTGGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCtt           <  1:167141/238‑1 (MQ=255)
                                                                                                                                                                                                                               ggTTTTTGCCGGTTTACTGAAGGATTTGCTGGCAGGTGAAATCCTGCAAGTGGCCTGCTGGCTGCTTACCCAGAAAGTGAACTGCCGCGATTAGTACCTGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGGGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACt        <  1:219389/238‑1 (MQ=255)
                                                                                                                                                                                                                                     tGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAGCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGt  <  2:315131/239‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
ATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGCCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAATCCTGCCAGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTGCCGCGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTTCCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGT  >  NC_000913/159032‑159495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: