Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 159,258 | G→T | 20.5% | V285V (GTC→GTA) | gluQ ← | glutamyl‑Q tRNA(Asp) synthetase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 159,258 | 0 | G | T | 20.5% | 85.3 / 5.8 | 61 | V285V (GTC→GTA) | gluQ | glutamyl‑Q tRNA(Asp) synthetase |
Reads supporting (aligned to +/- strand): ref base G (19/28); new base T (11/2); total (31/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.02e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGT > NC_000913/159032‑159495 | aTCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCGCCCAGTTTTT‑AAGGcggactga > 2:173883/1‑239 (MQ=255) tCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TCAGCCG‑CCCGGTTTTTACGGGTGAACGAAgagttt > 1:129313/1‑234 (MQ=255) aCCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTTCCGACTCGGGCACGGCGG‑TAAGCCG‑CCCGTTTTTGACGGCTTGCTGAAGGATTTGc > 2:374885/1‑239 (MQ=255) gtgGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAACCG‑CCAGTTTTTTAAGGCTCACCTAAGGGTTTtctcc > 2:291216/1‑237 (MQ=255) ggACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑GAAACCC‑CCCGGTTTTGTCGGCTGGCCAAAGGATTTgtcccggc > 1:505020/1‑232 (MQ=255) gcttttttaGCCTCTTTTTCCCTGCGTCGTAGGCCCTTGCGGAAAAAATTAGGCACTGGGGTAAAAATAGTACACCTC‑GGT‑AGTGTAAAACCTCATTCAGGACAAAAAAATAGCGGCCAATCATAGCTCCGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑Gc < 1:137578/231‑1 (MQ=255) cgGTTTAGCCCCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACCCCTC‑GGT‑AGTGTCAAACATCATTCAGGAAAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGcccc < 1:291216/239‑1 (MQ=255) gCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGCCGG‑TAAGCCG‑CCCGTTTTTTACGGCTCACTGAAGGGTTTTCTCGCCCCGTAAAT‑CCT‑GCCAAGTG‑GCTTCTTCCGCCTGCCCCAg > 2:507532/1‑239 (MQ=255) tttgggcccTTGGGGAAAAAATTGGCGACTCGGGTAAAAATAGTCCCCCCC‑GGT‑AGTTGCAAAAATGATTCAGGACAAAAAAATAGCGTGTAATCATAGCCCAGCATGACGCATTTGAGAAATTTGAATTTAAAATTGCCGACTCGGGCACGGAGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGa < 1:700223/232‑1 (MQ=255) taaccccTTGCGGCAAAAATTAGCGAATCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGaaa < 2:129313/234‑1 (MQ=255) tGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAACCG‑CCAGGTTTTTACGGCTGACTTAAGGGTTTTGCCCAGGCCGAAAA‑CCC‑GCC‑AGGGGGGTTTTGCCTCCTGCCCCCGaaaattaaatg > 1:181107/1‑231 (MQ=255) aaaaatTTAGGCACCCGGCTAAAAATGGTACCCCAG‑GGTGGGTGTC‑AACCTCTTGCAGGAAAAAAAAATGGCGGCTAAGCAGAGCTCGGAATGACTCTTTTGAGAATGTGGAATTGACAATTGCAGACTCGGGCACGGTGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGAGGCTGAAAT‑CCT‑GCC‑AGTGGGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑cc < 1:642018/235‑1 (MQ=255) aGCCACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCGAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTAc < 2:732943/239‑1 (MQ=255) aaaaaTAGTACACCGC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGAt < 2:181107/238‑1 (MQ=255) aaaaaaGTACCCCTCGGGT‑AG‑GTCAAAACGTAATCAGGACAAAAAAATAGCGGGTAAGCAAAGCTCAGGATTGAGCATTTGAGAATTGTGAATTTACAATTGCCGACTCGGGCACGGTGG‑TAAGCCG‑ACAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCg < 2:457678/239‑1 (MQ=255) aTAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑GAAACCG‑CCAGTTTTTGTCGGCCGCCGCAAGGAATTTGTCGGCGCTGAAAA‑CCT‑TCC‑CATGGGTCTTTGCCTGTGGCCCCCGAAATAGAA‑TG‑ACGCCGATTATAACCGGGGGGGGagagc > 1:722752/1‑233 (MQ=255) tACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTTAACCCG‑CCCGTTTTTTA‑GGCTGACCTAAGGCTTTGCTCGGCGATGTAATCCCT‑GCC‑A‑TGGGCTTTCCCCTCCTGCCCCCCAAATTTAAATGCCCG‑CG‑TTGGAACCGGGCGTgggaccccattg > 1:382724/1‑228 (MQ=255) tACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCCGAAATTGAAGTG‑CCG‑CGATTTGTACCTGGCGTGGATCCCCttttg > 2:882130/1‑237 (MQ=255) tACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGGTTTTTACAGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCCAAGTG‑GCTTCTGCCTGCGGCCCCAGCAATTTGAGTG‑CCG‑CGATTAGGACCGGGGCTGGTACCCCTTTCg > 2:390476/1‑239 (MQ=255) accccTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCAGAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCgg < 2:828460/236‑1 (MQ=255) cTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGGTTTTTACGGCTGACTGAAGGGTTTTCTCGACGCTGGAAT‑CCT‑GCC‑AGTGTGCTTCTTCCTGCTGCCCCAGAAATTGAAAGG‑CCG‑CGCTTTATAACCGGCGGGAGTCGCCTTTCGGCaa > 2:345646/1‑238 (MQ=255) cTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGGTTTTGACGGCTGACTGAAGGAGTTGTCCGACGCTGGAAA‑CCT‑TCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTTAAGTG‑CCG‑CGGTTAGTACCGGGCGTGGATCCCCTTTCGGcca > 2:401375/1‑236 (MQ=255) c‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTTACCGCTGACTTAAGGATTTGCTCGCCGCTTAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCCGAAATTTAAGTG‑CCG‑CGATTACAACCGGGCGTGGACTGCCATTCGGCacagc > 1:190364/1‑235 (MQ=255) tggT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGc < 2:473027/238‑1 (MQ=255) t‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑CGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGGTCGCCTTTCGGCAACGCAg > 2:199665/1‑238 (MQ=255) t‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTTGAGTG‑CCG‑CGATTATTACCGGGCGTGGATCCCCTTTCGGCCACGCAgg > 2:4679/1‑239 (MQ=255) tgtCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCCCGACGCTGAAAT‑CCT‑TCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGGG‑CCG‑CGGTTAGGACCGGGCCTGGATCCCCTTTTGGCAACGCAGgggc > 2:846874/1‑239 (MQ=255) tgtCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAACCG‑CCCATTTTTGACGGCTGATCGAAGGGTTTGCTCGCACCCGAAAC‑CCT‑GCC‑CGGGGGCTTTTCCCTGCTTCCCCCGAAAATAAAAGG‑CCG‑CGGTTAGTAACGGGCGGGGAGTCCCTTTCGGGaaaacaagggg > 1:235419/1‑230 (MQ=255) catTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAAGG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGGGCATGGTTCt > 2:547653/1‑237 (MQ=255) ttCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCCACGCAGGCGCATGATTTTGCtt > 2:708118/1‑239 (MQ=255) aGGAAAAAAAAAATGCGGATAATCACAGCCCAGCATGACTCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGa < 1:547653/239‑1 (MQ=255) gACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGaaa > 2:167141/1‑239 (MQ=255) gACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAAGG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCCACGCAGGGGCATGATTCTGCTTGGaaa > 2:710151/1‑239 (MQ=255) aaaaaTAGCGGCTAATCATAGCTCGGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑cc < 2:197616/173‑1 (MQ=255) aaaaaGCGCGTCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTa < 2:668638/239‑1 (MQ=255) aaaaTAGCGGCTAAGAAAAGACCAGCATGCCGCATTTAAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAg < 2:427961/239‑1 (MQ=255) aaatagggtcatatcattcgtCAGCATGCCTCATTTGAGAATTTTGAATTTACAATTGCCGCCTCGGGCACGGCGG‑TAGGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAACT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAgc < 1:649056/219‑1 (MQ=255) aaaTGGCGGTTAAACATAGCCCAGCAGGCAGCATTTGATAATGTTTAATTTCCAATGGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACGGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAgc < 1:456173/239‑1 (MQ=255) cGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTTACGGCTGACTGAAGGATTTGCTCGACGCTGGAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCGGCCCCCGAAATTGAAGTG‑CCG‑CGGTTATTACCGGCCGTGGGTCGCCTTTCGGCAACGACGCGGCAGGATTCTCGCTGGAAAGATTAACGCCttt > 1:98434/1‑238 (MQ=255) ccaaagccccggaTGACGCTTTTGGGATTTTTAAATTTACATGTGCCGGCCCGGGCACGGCGG‑TAAGCCG‑CCTGTTTTTGACGGCTGACTGAAGGATTTGCCCGACGCTGAAAT‑CCT‑GCC‑AGTGGGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTa < 1:852710/227‑1 (MQ=255) gCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTTTGGATTAAGGGCCAACg > 2:168813/1‑239 (MQ=255) gtggACGTATTTGAAAATTGTGAATTTACAATTGCCGGCTGGGGTACGGAGG‑TAAGCCG‑CCGGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTTAAAT‑CCT‑TCC‑AGTGTGCTACTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCgg < 1:184561/236‑1 (MQ=255) cATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAACGTTTAGCGCCTTTTGGGTTAAAGGCCAGCgg > 2:399549/1‑239 (MQ=255) cATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGGACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGATTATCGCCTTTTGGATTAAGCGCCAGCgg > 2:810868/1‑239 (MQ=255) atggcggttttGAGAATTTTGAATTTACAAATGCCGTCTCGGGCCCGGCGG‑TAAGCCG‑CCAGTTTTGGCCTGTTGATGGAAGGATTTGCTCGAAGCGGAAAT‑CCT‑GCC‑AGTGTGCGTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCgg < 1:173883/230‑1 (MQ=255) cGCATTTGAGAATGTTGAATTTCCAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGa < 2:118418/238‑1 (MQ=255) ttGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGGAACGCAGGCGCATGATTCTGCTTGGAAAGTTTTGCGCCTTGGGGATTAAGGGCCAGCGGCAGGTGAATg > 2:489655/1‑239 (MQ=255) gaATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCCGGCGGGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGTTTAAGCGCCAGCGGCAGATGAATGTaa > 2:851717/1‑238 (MQ=255) tGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGGGTGCTTCTGCCTGCTGCCCCAGAAATTGTAGGG‑CCG‑CGATTAGTACCGGGCGTGGGTCGCCTTTTGGGAACGCCAGGGCATGATTCTGCTTGGAAAGGTTAGCGCCCTGTGGGTTAAGCGCCAGTGGCaacaggaaggaatttggg > 1:306900/1‑221 (MQ=255) aCAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑ACAGATTTTTACGGCTGGCTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCCGAAATTGAAGTG‑ACG‑CGATTAGTACCGGGCGTGGATCGCCTTTCCGCAACGCCGGAGCATGGTTCTGCCTGGAAAGTTTAGCGCCTTGTGGTATAAGCGCCACCGGCAGATACATGTAAACTGGGACTTT‑CCa > 1:169156/1‑239 (MQ=255) tGCCGACTCGGGCGCGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCaa < 2:190364/239‑1 (MQ=255) aCGGGGG‑TAAGCCG‑CGAGTTTTTGGAGGGTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAg < 1:199665/239‑1 (MQ=255) cggggg‑TAAGCCC‑GCAGTTTTGGACGGGTGACTTAAGGATTTGGTCGAAGCTGAAAT‑CCTGGCG‑AGT‑TGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAg < 1:374885/238‑1 (MQ=255) ggcgg‑TAAGCCG‑CCAGGTTTTTACGGCTGACTGAAGGGTTTGCTCGACCCTGAAAT‑CCT‑GCC‑AGGGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGGGGATCGCCTTTCGGCAAAGCAGGCGCATGATTCTGCTTGGACAGTTTAGCGCCTTGTGGGTTAAGAGCCAGCGGCCGATGCATGTAATCTGGCCCTTTCCCCGCCaaaaaacgggaaaacg > 1:33688/1‑228 (MQ=255) gccg‑ccATTTTTGGGCGGCTGTGTGAAGGTTTGGCTGCACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGGCTGGTGCCCCAGAAATGTAAGTG‑CCG‑CGATTAGGACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTg < 1:345646/238‑1 (MQ=255) ccg‑ccAGTTTTTTACGTCTGACTGAAGTATTTGCCCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTGcc < 1:168813/239‑1 (MQ=255) cccccgTTTTTTGCGGTTTACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTGCCt < 1:791552/234‑1 (MQ=255) ccccAGTTTTTGGCGGCTGACTGAAGGATTTTCCCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTGCCt < 1:882130/237‑1 (MQ=255) cccATTTTTTGCCGGTTGACTGAAGGTTTTGCTCGACGTGGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTGCCtt < 1:167141/238‑1 (MQ=255) ggTTTTTGCCGGTTTACTGAAGGATTTGCTGGCAGGTGAAAT‑CCT‑GCA‑AGTGGCCTGCTGGCTGCTTACCCAGAAAGTGAACTG‑CCG‑CGATTAGTACCTGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGGGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACt < 1:219389/238‑1 (MQ=255) tGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAG‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGt < 2:315131/239‑1 (MQ=255) | ATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTC‑GGT‑AGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGG‑TAAGCCG‑CCAGTTTTTGACGGCTGACTGAAGGATTTGCTCGACGCTGAAAT‑CCT‑GCC‑AGTGTGCTTCTGCCTGCTGCCCCAGAAATTGAAGTG‑CCG‑CGATTAGTACCGGGCGTGGATCGCCTTTCGGCAACGCAGGCGCATGATTCTGCTTGGAAAGTTTAGCGCCTTGTGGATTAAGCGCCAGCGGCAGATGAATGTAATCTGGCACTTT‑CCAGCCAAAAAGCTGGTACAGCGAGATTTGCCTTACTGTTGGT > NC_000913/159032‑159495 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |