| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,523,991 | A→G | 15.0% | S375G (AGT→GGT) | mrcA → | penicillin‑binding protein 1a, murein transglycosylase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,523,991 | 0 | A | G | 15.0% | 176.8 / 3.4 | 78 | S375G (AGT→GGT) | mrcA | penicillin‑binding protein 1a, murein transglycosylase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (27/37); new base G (10/3); total (38/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGC > NC_000913/3523754‑3524221 | cagcagGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGAGGCTGGCGGACGGGTCGACCGTCGCCTTGAg > 2:237900/1‑239 (MQ=255) ccGCGGAGCAGGCGGTACGTAATAACGGGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATgg < 2:164111/237‑1 (MQ=255) gcagaaGGCGGTACGTAATAACGGTCTGGACTAAGACATGCGCCAGGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGGGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCg < 2:863769/239‑1 (MQ=255) tggCGGTAGGTAATAACGTGTTGGACTACGACATGCCGCCCGGGCATCGCGGGCCGGCAAATGTGCTGTGCAAAGGGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGAAACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGc < 1:468315/238‑1 (MQ=255) agGCGGTTCGTAATACCGTGCTGGACGACGACAGGCGCCACGGCTAGCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCg < 2:130869/238‑1 (MQ=255) ggcaCGTAATAACGTGCTGGACTACCACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTTGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCCCCAGCGCCCATCCTCAGCAAGCGACGCCGAGGCTGGTGGCGGGGGCGGCCGTCGCATTGAGGAGGGAAGGGGttgtggggg > 1:577558/4‑232 (MQ=255) aCGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGGCGGGCCGACCGTGGCATTGGCAAGGGAAGGGGTTGGGGGG‑gcgc > 1:537951/1‑239 (MQ=255) aCGTAATAACGTGCTGGACTACGACATGCGCCAAGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑gcg < 2:409090/238‑1 (MQ=255) gTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGCCGGGTCGACCGTCGCCTTGAGTATGGAAGGCGTTCGCTGG‑Gcgcg > 2:553807/1‑238 (MQ=255) taaCGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGCTACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCCATCCTCAGCAAGCGACGGCGATGCCGGCGGACGGGTCGACCGTCGCATTCAGTAGTGAAGGGGTTGCCTgg‑ggcgggcct > 2:697172/1‑230 (MQ=255) cGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGCCGGGCATGCTGGCGGACGGGTCGACGGTCGCCTTGAGTATGGAGGGCGTCCGCGGG‑GGGCGGCCTTAcg > 1:854848/1‑238 (MQ=255) cGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGGCCTTAcc > 2:474851/1‑239 (MQ=255) cGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAATCACCAGCGCCAATCCTCCGCAAGCGACGGCGGTGCTGGCGGCAGGGGCGAACGGCCCCTTGAGAAGGGAGGGGGGCCGCTGGTGCGCG‑CCTTAcc > 1:739856/1‑239 (MQ=255) tGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGCCGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGGGTATGGAAGGCGTTCCTTGG‑GGCCGTCCTTCCCGt > 2:227570/1‑239 (MQ=255) tGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGGGTATGGAAGGCGTTCGCTGG‑GCCCGTCCTTACCGt > 2:17239/1‑239 (MQ=255) gCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGGGGATGGAAGGCGTTCCCTGG‑GCGCGGCCTTACCGt > 2:433031/1‑238 (MQ=255) ttgggtgtggcagcgcgcCAGTGCGATCGGGGCCCGGCAAATTGGGTGTGGAAAGGGGGCCGGTCGGCGGGGGATAACAAAAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCGGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGtt < 1:796235/224‑1 (MQ=255) tggggttACGAAATGCGCCGCGGGTTGCGGGGCCCGGCAAATGTGTTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTCCCGATACGCTGAAGGCGCTGCCAACCTATGGGCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTc < 1:237900/233‑1 (MQ=255) cTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGGTAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGGCCGCTGCGGCCTGCCGCAGTCCCCAGCGCCAATCCCCACCGAGCGACGGCGCTGCGGTCGGGCGGCTCACCCGGCGCACTGGGGTCGGAACGCGGCCGCTGG‑GCGCGCCCCTACCGtgt > 1:715715/1‑237 (MQ=255) tGGACTTAGAAATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGGGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTc < 2:556067/238‑1 (MQ=255) tGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCg < 2:7972/239‑1 (MQ=255) aCGACATGCGCCACGGCTAACGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACt < 2:856642/239‑1 (MQ=255) cGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCCATCCTCAGCAAGCGACGGCGATGCTGGCGGCCGGGTCTACCGTCGCCTTGGGAGTGGAAGGCGTTCGCCGG‑GGGCGTCCTTACCGGTCGGGAACTc > 1:774255/1‑239 (MQ=255) tggcccgcggggtttCGGGGCCCGGCAAATTGTCTGGGGAAAGTGGGCGGGTCGGCGTGGGATACCAAGAATATTACCGATTCGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCa < 1:352012/225‑1 (MQ=255) tGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGCTGCTGGCGGACGGGTCGCCCGTCGCATTGAGAAAGGAAGGCGTCCGCCGG‑CCGCGCCCTTACCGTTCGGATACCCACCaa > 1:62985/1‑239 (MQ=255) tGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGATGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTTG‑GCGCGTCCTTACCGTTCGGATACTCaccca > 2:440803/1‑235 (MQ=255) tGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCaa > 2:625510/1‑239 (MQ=255) tGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCCCGTCCTTACCGTTCGGATACTCAGCaa > 2:791164/1‑239 (MQ=255) tGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGAGACGCTGAAGGCGCTACCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGCCGGCGAAGCTGGGCGGCGGGGCGACCGGCGCCTTTGGAATGGAAGGGGGTGGCTGG‑GCGCGTCCtacaccgccgggaaatcaccag > 1:526197/1‑218 (MQ=255) tGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGAAACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGGCGGCCGGCCCATTGGGTCTGGAAGGCGGTAGCCGG‑GCCCGGCCTTACCGCCCGGaatccaagcaa > 1:540296/1‑229 (MQ=255) cTATCGCGGCCCGGAAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGAcg < 2:887127/238‑1 (MQ=255) gCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTAGCTGG‑GCCCGTCCTTACCGTTCGGATACTCAGCAAGGCCCGCCGCCGCGTaaa > 2:178938/1‑239 (MQ=255) gCCCGGCAAATGTGCTGTGGAAAGTGGACGAGTCGGCGTGGGATAACAACCAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGTCGATGCTGGGGGCACGGTCGACCGGCGCATTGGGTATGGAAGGCGTTCTCTGG‑CCGCGGCCTTACCCGTCGGCAACCCAACAAGGGCCGaagggggcgaaa > 1:879196/1‑228 (MQ=255) cTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGAATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCTGAAAGGGACCGCTGt > 2:415987/1‑236 (MQ=255) tgtgGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCCCGTAAAGTGACCGAGGtctt > 2:694734/1‑235 (MQ=255) cGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAg < 2:672889/238‑1 (MQ=255) cgggttGGGATAACAAAAAGTTTCCCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCGGCCGCAGGCACCAGCGCCAAGCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAAt < 1:276526/233‑1 (MQ=255) aaGATTACCGATCCGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGAGCGCGATGCCGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTgg < 2:858796/239‑1 (MQ=255) aGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGAGACTCAGCAAGGACCGACGGCGCGTAAAGTGACCGATTTTCTGCAAACGGGGCCACAAATCTGGGTGCGACCGGGTggg > 1:401611/1‑238 (MQ=255) aGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCCCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGGAAAGGGACCAAGGTTTTGCAAACGGGTCAGAAAAACGGGGTTCGACGGGGTggg > 1:826970/1‑238 (MQ=255) aTTACCGATACGCTGAAGGCGCTGCCAACCTATGGGCCGCTGCGGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGa < 2:112425/239‑1 (MQ=255) tACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGt > 1:86586/1‑208 (MQ=255) tACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGt < 2:86586/208‑1 (MQ=255) gATACGCTGAAGGCGCGTCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGGGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCAtg < 2:630239/239‑1 (MQ=255) cttccccTGACGGGGCTGCCAACCTTTGGGCCGCGGCTGCCTGCCGCAGTCCCCGGCGCCAATCCCCAGCAAGTGACGGCGATGCTGGCGGCCGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCAtg < 1:301616/233‑1 (MQ=255) aTACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCTCTGG‑GCGCGGCCTTTCCGTTCGGTCACTCAGGCAGGACCTGCGCCGCGGAAAGTGTCCACTGCTCGGCAAACCGGGCCACAAATTCGGGGGCGACAGGTTgggggtggcatg > 1:833484/1‑229 (MQ=255) ccGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCCCCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCAtggtg < 2:584342/238‑1 (MQ=255) cGCTTAGGGGGCTGCCCCCCTATGGTCCGCTGCGGCCCGGCGCAGTCCCCAGCGCCAATCCCCAGCAAGCGACGGCGATGCTGGCGGCCGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCAtggtgg < 1:625510/239‑1 (MQ=255) ggAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGGAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGtggct < 2:14787/237‑1 (MQ=255) aaGGCGCTGCGAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGGGTTTGGAAGGCGTTCGCTGG‑GCGCGGCCTTACCGTTCGGTTACTCAGCAACGCCCGCCTCCGCGTAAAGGGACAGACGTACTGCCAACGGTGGCGCAAATCTGGGGTCGCCAGGTTGGCCATGCAtgggggggggc > 1:607312/1‑234 (MQ=255) cgcgGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGcaca < 2:778982/238‑1 (MQ=255) gTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTGTGGAAGGGGTTCGCTGG‑GCGCGTCCTTACCCTTCGGGTACTCAGCAAGGACCGACGCCCCGTAAATTGACCGAGGTTCTGCAAACGGGTCACCAATCCTGGGTTCGCCAGCTTGGCGCTGAATGGGGGCTGGGCCAAGGGCgggggagaagc > 1:213736/1‑231 (MQ=255) gTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCCAATCTGGGTTCGTCAGGGTGGCGATGGCTGGGGGCTGGCAAAAGTGCCGGAAGGGAAc > 1:199985/1‑239 (MQ=255) ccGCTGGCGCCTGGCGGAGTCACCAGCGTCAATCCTCAGCAAGCGGCGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACt < 2:888086/238‑1 (MQ=255) cGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGGATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGAGACTCAGCAAGGACCGACGCCGCGTAAAGGGACCGATGTTCTGCAAACGGGTCAGTCAATCTGGGGCCGTCAGGTTGGCGATGCATGGTGGCTGGACCAAATGCCGGAAGTGAAATca > 1:54227/1‑238 (MQ=255) cttggtccctgccGCTGTCACCAGCGCCAATCCTTAGACAGGGACGGGGATGCTGGCGGACGGGGCGACCGTCGCATTGAGTATGGAAGGCGTGCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCgg < 1:433031/232‑1 (MQ=255) ctgctgCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGCCGCCGCGTAAAGGGACCGATGTTCTGCAAACGGGTCAGCAAACCTGGGTTCGTCAGGATGGGGCGGCATGGGGGCTGCAACAAAATCCGGAAGGAAACTCggg > 1:31988/1‑238 (MQ=255) ctgctgCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGTCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACCCGgc > 2:672837/1‑239 (MQ=255) tctgccggcCGGCTTCCCCAGGGCAAATCCTGCGGAAGCGGCGGGGGTGCTGGGGGACGGGGCGACCGTCGCATTGAGTATGGAAGGCGTTCGGTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTTAGCAAATCTGGGTTCATCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGgcgc < 1:798507/238‑1 (MQ=255) gctgcCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAg < 2:718241/190‑1 (MQ=255) gctgcCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGGGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAg > 1:718241/1‑190 (MQ=255) gctgcCTGCCGCAGGCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTTGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGgcgc < 2:526197/239‑1 (MQ=255) cgtgcccgccccgctCACCCGCGCCAAACCCCAGCAAGGGGGGGCGATGCGGGGGGGCGGGTCGACCGGCGCATTGAGTATGGAAGGGGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGgcgc < 1:679604/225‑1 (MQ=255) tgcctgccGCAGTCACCAGCCCCAATCCTCAGCAGGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTg < 2:616813/239‑1 (MQ=255) cctgccACAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACTGGTCAACCGTCGGATTGTGAATGGAAGGCGTCCGCTGG‑GGCCCGCCTTACCGCTCGGATACTCAGAAAGGACCGACTCGGCGTAAAGGTCCCGGTTTTCTCCACATCGGACACCAAACCTGGGGTCGCCAGCTTGCCGAGGCATGGGGGCGAGCACACATACCGGaggggagcccacggaggag > 1:249279/1‑221 (MQ=255) cGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCGGGCACAAGGGCCGGAAGTGAACTGGGCGCTGGGGTCgt > 2:519968/1‑238 (MQ=255) aatccccAGCGCCAATCCGCAACAAGGTGCGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCa < 2:540296/234‑1 (MQ=255) gcCAATCCTCAGCAAGCGACGGCGATGCTGGCGGGCGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCaaaa < 2:66021/239‑1 (MQ=255) aatcccgaggagccGACGGCGATGCTGGCGGACGGGTCGACCGTCGCGTTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGGCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACgg < 2:617108/226‑1 (MQ=255) aaTCCTACGCAAGGCGCGGCGGTGCCGGCGGACGGTTCGGCCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACgg < 2:318871/239‑1 (MQ=255) aGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCCAAACGGTGCCGtt > 2:726627/1‑239 (MQ=255) gCAAGCGACGGCGATGCTGGCGGGCGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTa < 2:537951/239‑1 (MQ=255) gggggcGGGTCGCCCTTCGCATGTGGTTTGGAGGGCGTTCTGCGG‑GCGCTTCCTTACCGTTCGGTTCCTCAGCAAGGACCGACGCCGGGTAAAGTGACCGATTTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCtt < 1:672837/234‑1 (MQ=255) gggACGGGGCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCtt < 2:774255/237‑1 (MQ=255) cGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCtt < 2:854848/238‑1 (MQ=255) cggtgCATTGGGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCaga < 2:401611/235‑1 (MQ=255) cGTCGCATTGGGTAGGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCaga < 1:474851/239‑1 (MQ=255) tCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGTTCAATCCGCAAAACGGTGCCGTTATGGCGCTGGCCGGTGGCGTTGATTTCaacaaaaga > 1:748215/1‑232 (MQ=255) | CAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGG‑GCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGC > NC_000913/3523754‑3524221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |