Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,551,915 A→C 23.5% V186G (GTG→GGG)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,551,9150AC23.5% 76.0 / 4.1 52V186G (GTG→GGG) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (24/14);  new base C (5/9);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCG  >  NC_000913/3551681‑3552143
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      
gCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGCCAACGGGGAGATCCCACGCTTGACCGGTAAATGTAAACCCCGGCTCCCAGCGTCCGTCTTTCGGCCCttt                                                                                                                                                                                                                                    >  1:120346/1‑239 (MQ=255)
gCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGGGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGCCTTTCGTCACttt                                                                                                                                                                                                                                    >  2:42828/1‑239 (MQ=255)
gCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCCCttt                                                                                                                                                                                                                                    >  2:737597/1‑239 (MQ=255)
                 tGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGGAATGGTAAACTCCGGCCCCCAGCGCCCGTCTTTCGTCACTTTACCGCCAAACCCAAccc                                                                                                                                                                                                                   >  1:394958/1‑238 (MQ=255)
                        agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACAt                                                                                                                                                                                                             >  2:811019/1‑238 (MQ=255)
                        agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGAATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCTTCCGTCTTTCGTCACTTTACCCCCAATCCCTACCTGCACAt                                                                                                                                                                                                             >  2:607030/1‑238 (MQ=255)
                                  tCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCaagccttcg                                                                                                                                                                                                  >  2:270965/1‑231 (MQ=255)
                                    aGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCCCATCCAGTGCTTCGtt                                                                                                                                                                                                >  2:657840/1‑239 (MQ=255)
                                                         tgctgTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCCCGGGTAGtt                                                                                                                                                                           >  2:497727/1‑239 (MQ=255)
                                                                   cACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCCCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTGCGTTGTTGCGGaaaccagggggattaaacgggat                                                                                                                                                                  >  1:779716/1‑217 (MQ=255)
                                                                   cACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGCCTTTCGTCACTTTACCCCCAATCCCTACCTGCACATCCATGGCTTCGTTGTGGCGGAACCACGGGCAGCTatctcgctg                                                                                                                                                                  >  2:222505/1‑230 (MQ=255)
                                                                        aaGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCCCATCCCGTGCTTCGTTGTGGCGGAACAACGGGAGGTTCATGCGATGCCatac                                                                                                                                                            >  2:156689/1‑236 (MQ=255)
                                                                                      gtTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACCGGTAGTTACTCGGATGCCACACATACGGCCGTTCa                                                                                                                                              >  2:479395/1‑238 (MQ=255)
                                                                                               aGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGCCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCACCCTGTTTg                                                                                                                                     >  2:170689/1‑238 (MQ=255)
                                                                                                gccAGATAAAACGGATGGCGGGGGGTCGCCGGCCAAAGGCGACGCGGCTGCGCCCCGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGcc                                                                                                                                   <  2:394958/237‑1 (MQ=255)
                                                                                                               tGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCCCTGTACCGCCAATACCACCCTGCCCATCCAGTGCTTCGTTGTGCCGGAACCACGGTGATTTACAGCGATGCCCGCAACCGGCGCGTTCAACCCGTTTGcccaccccaaaaaaacg                                                                                                                    >  1:621118/1‑224 (MQ=255)
                                                                                                               tGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCCCATCCAGTGCTTCGTTGTGGGGGAACCCCGGGTAGTTACTGCGATCCCCGTCATCCCGCGCTTCAACCCGTTTGCCATCCAAAAAAAAccg                                                                                                                   >  1:43007/1‑237 (MQ=255)
                                                                                                               tGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACTTCCAGGGCTTCGTTGTGGCGGAACCCAGGGTCGTTATTTCGGTTCCCTTCCTCCCGCGCTCTAACCCGTTGTCCGTCGACAAAAAAcgc                                                                                                                   >  1:340779/1‑237 (MQ=255)
                                                                                                               tGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGCCATCCGGCCCTTCAAACCGTTTGACCTCGCACaaaaacgg                                                                                                                    >  1:716271/1‑235 (MQ=255)
                                                                                                               tGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGCCTTTCGTCCCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCCAGTCCAGTCATCCGGCACTTCAACCCTTTTGcccatcaaacaaacagt                                                                                                                    >  1:218685/1‑224 (MQ=255)
                                                                                                                 gcgttccgcccccTGCAACAGACGCGGGGCGTGCGCCCCGCCTTTCGGAGAGCCGACAGCGGGGGGATCCCACCCTTGCCCGGAAATGGAAACCTGCGGCCCCACGCCGCCGTCGTGCTTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTgg                                                                                                                  <  1:156689/227‑1 (MQ=255)
                                                                                                                         tccccTGCCACAGCCCGCGCGGCTGCGCACCGCCATTCCGAAAGCCGACAACGGGGAGATCCCACGTTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCCCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACaa                                                                                                          <  2:301263/235‑1 (MQ=255)
                                                                                                                          cgccccccccagcccggggcgCTGCCCCCCGCCTTTCCGAGCGCCGACAGCGGGGAGCCCCCCCGGTTCCCCGATAAGGGAAACCTCCGGCTCCCACCCTCCGTCTTTCGTCACTTGACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAAc                                                                                                        <  1:42828/219‑1 (MQ=255)
                                                                                                                          ccctctccccagcggccggggCTGCGCCACGCCATCACTATGGCCGGCGTGGGGCAGCCCCCTCGTTTAGTCGGTTATGACACTCCCCGGCTCCCGACGCCCGGTTTTCTTCCGTTTGCCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAAc                                                                                                        <  1:782699/220‑1 (MQ=255)
                                                                                                                               taaaggggacaggcgccgcgcccacgccaTTACGATAGCCGACAACGGGGAATTCCCCCGCTTGCCCGTTAATGGTAAACTCCGGCTCCCAGCTTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCatat                                                                                                   <  2:838477/218‑1 (MQ=255)
                                                                                                                                ccACAGACGCAGCGGCTACGCCACGCCATTACGATAGCCGACAACGGGGCGATCCCACGCTTGACCGGTAATGGTAAACTCCCGCTCCCAGCGTCCGGCTTTCGTCACTTTACCGCCAATACCTACCTGCACATCCAGCGCTTCGCTGTGGCGGAACCACAGGCGGATACCGCACGTCCCGTCATTCCGCGCTTCACACTGGTTGCCCACGCCAAAAGAAAGGGGGGACAAACCacaaa                                                                                                  >  1:693631/1‑235 (MQ=255)
                                                                                                                                          gcgggtggcccccccctatttggATACCCGACACCGGGGAGATCCCCCGCTGGACCGGTAAGTGTAACCCCCCGCTCCAAGGCTCCGTTTTTGCTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCa                                                                                         <  1:737597/216‑1 (MQ=255)
                                                                                                                                          aGCGGGCGCACCACGCCATTACGATAGCCGACAACGGGGAGATCCCCCGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGCCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCa                                                                                         <  2:576652/238‑1 (MQ=255)
                                                                                                                                          aGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCCCGGGGAGTTACTGCGATGCCAGTCATCCGCGGCTTCAACCTGTTTGCCATCGCAAAAAAACGGGCGGGAAAAAcacaaatgtaagattaa                                                                                        >  1:753503/1‑223 (MQ=255)
                                                                                                                                                                    gCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTTCCATCGCCAAAAGACTGGCGGAACAAACCATATTTTTAGTTCCAACCCTAACCCCTCGCCGACTGaacca                                                              >  2:567557/1‑235 (MQ=255)
                                                                                                                                                                    gCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCCTATTGATAGTTCAGACCGTAAcccccgccagcatggccgc                                                              >  2:53409/1‑223 (MQ=255)
                                                                                                                                                                    gCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGCTAATTCAGACCGTAACCCGTCGCAGACTggacgc                                                              >  2:628929/1‑234 (MQ=255)
                                                                                                                                                                     ccgcaccgggggAACTCCCCCGCTTACCCTGTTATGGAAACCCCCGGCCCCCAGGTCCCGGTTTTCCTCCCTTGACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGAc                                                             <  1:657840/231‑1 (MQ=255)
                                                                                                                                                                         acaaggggAGAATCCCACGCTTGCCCGGTAAGGGTAAACCCCGGCCCCCAGCTTCCGTCTTTCGTCCCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGt                                                          <  2:753503/235‑1 (MQ=255)
                                                                                                                                                                          aaCGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGGAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATTGTTCAAAACGTAACCCCTCCCAGAGTAACCGaaaatt                                                         >  1:577118/1‑233 (MQ=255)
                                                                                                                                                                               ggACATCCCACGCTTGGCCGGTAATGGTAACCCCCGGCTCCAAGCGTCCGTTTTTGCTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCAt                                                    <  2:751553/238‑1 (MQ=255)
                                                                                                                                                                                 agaTCCCACGTTTGACCGGTAATGGTAAACCCCGGCTCCCAGCGTCCGTCTTTCGTCCTTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTg                                                  <  2:120346/238‑1 (MQ=255)
                                                                                                                                                                                              tgccccgaaaTGGTAACCCCCGGCTCCCGGCGTCCTTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCa                                     <  2:253684/231‑1 (MQ=255)
                                                                                                                                                                                              tgcccGGTAAGGGTAACCTCCGGCCCCAAGCGTCCTTTTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCa                                     <  2:43007/236‑1 (MQ=255)
                                                                                                                                                                                              ggACCGTTAATGGAAAACTCGGGCCCCCGGGTGCCGTTTTTCTCCACTTCACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCa                                     <  2:175304/238‑1 (MQ=255)
                                                                                                                                                                                                aCCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATTCCAGTCCTCCGGCGCTTCAACCTGTTTGCCATCCACAAAAGACTGGCCGCACAAACCATATTGTTAGTTCCTCCCGTCACCCCCCCCACACTACCCCACAAGTGCCAtttttcccgggaaacac                                    >  1:504633/1‑223 (MQ=255)
                                                                                                                                                                                                    gTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACCAAAGACTGGCGGAACCAACCCTATTGATAGTTCAGACCGCAACCCGTCGCCGACTGACCGACAGTTGCCCTTGAGTCGCGGAAGGAcgccg                                >  2:37927/1‑238 (MQ=255)
                                                                                                                                                                                                        tgtaaacccccGGCCCCCAGCCTCCGTCTTTCGTCCCTTTACCGCCAATCCCTACCTGCACATCCATTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGa                           <  2:556877/230‑1 (MQ=255)
                                                                                                                                                                                                        tGGTAAACTCCGGCCCCCAGCGTCCGTCTTTCGTCACTTTCCCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGa                           <  2:101090/239‑1 (MQ=255)
                                                                                                                                                                                                         ggTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCCGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCat                                                                                                     >  1:251949/1‑164 (MQ=255)
                                                                                                                                                                                                         ggTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCat                                                                                                     <  2:251949/164‑1 (MQ=255)
                                                                                                                                                                                                          gaaaaCTCCGCCCCCCGGTGTCCTTCTTTCGTCACTTTCCCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGa                           <  2:801855/235‑1 (MQ=255)
                                                                                                                                                                                                                  cGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCGGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGCAAACCGACGCACACTGACCGACAGTTGCCATTTAGTCGAGGAAGAAAGCCCCCAGACGGCCCAGa                  >  1:178592/1‑238 (MQ=255)
                                                                                                                                                                                                                     cccccggggtccatcTTGCTACACTTGACCGCCAACCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGacca               <  2:456438/230‑1 (MQ=255)
                                                                                                                                                                                                                       cccAGCTTCCTGTTTTCGTCCCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCg            <  2:577118/239‑1 (MQ=255)
                                                                                                                                                                                                                             ctccgtcTTTCGTCCCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTAcc       <  2:716271/237‑1 (MQ=255)
                                                                                                                                                                                                                                 gtcTTTCGTCCCTTACCCGCCAATCCCTACCTGCTCATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAgcg  <  2:58510/239‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      
GCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCG  >  NC_000913/3551681‑3552143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: