BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,565,286	T→G	13.8%	H432P (CAC→CCC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,286	0	T	G	13.8%	214.7 / 2.5	112	H432P (CAC→CCC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base T (50/39); new base G (2/19); total (53/59)
Fisher's exact test for biased strand distribution p-value = 7.91e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

ATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGGAGAAGA  >  NC_000913/3565049‑3565523

aTTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACCCCG‑TTGGTGA‑CGGTGGTGAAACGACCCGGG‑AGAATTTTCGCAAAGTCGGCAAACAACGATTCCACCATAGAATTAAGGTGCAGTGCCGATAAACCGTTAACTTtatg                                                                                                                                                                                                                                               >  1:485582/1‑239 (MQ=255)

              gttttttgcccgtgatttAAGCCGGTTAAGGTGTGTGCGCCATTTCGGGCCCAGCGTG‑CCGTCCAGCGCGGTTGAAGGGGATGGGTTCCCTCCCGCCAGCCAGCGCCGCGGCGTCACACCG‑TTGGCGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGTGGCTCA‑CACCAACCg                                                                                                                                                                                                                                <  1:881836/223‑1 (MQ=255)

                tttgttgCAGCTCATTAAGCAGGCTAAGGTCGGGGCGCCAGTTACGGCCCAG‑GTGTTCGGCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCg                                                                                                                                                                                                                                <  2:102143/236‑1 (MQ=255)

                    ttgCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATCCACCGTTAACTTTGTGGCTCA‑CAACACCCgccagc                                                                                                                                                                                                                           >  2:571721/1‑238 (MQ=255)

                    ttgCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GAGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCCGCGCCCCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTGGCCAAGTCGGCGAACAACAATTGCACCTTCAAATTATAGTGAAGTTCCGTTACACCTGTAACTTTTGGGCTCC‑AAAAAACGgccaaag                                                                                                                                                                                                                          >  1:511873/1‑236 (MQ=255)

                       cAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑CAGATTTTCGCAAAGTCTGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATAAACCGTTAACTTTGTGGCTCA‑CAAAAACggccggcccg                                                                                                                                                                                                                        >  2:68745/1‑236 (MQ=255)

                         gCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAGGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGAACCATCAGCTTAGAGTCCAGTTCCGATACACCGTTAAATTTATGGCACA‑CAAAAAACGCCAACCAGGcc                                                                                                                                                                                                                     >  1:304006/1‑239 (MQ=255)

                         gCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGcc                                                                                                                                                                                                                     >  2:114994/1‑239 (MQ=255)

                                      gCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCC‑CAACAACCGCCAGCCAGGCCATACGcaccctcc                                                                                                                                                                                                        >  2:643717/1‑234 (MQ=255)

                                       cTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTTA‑CAACAAACGCCAGACAGGCCATACGCACacgac                                                                                                                                                                                                        >  2:764285/1‑238 (MQ=255)

                                       cTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTCTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACacgac                                                                                                                                                                                                        >  2:12460/1‑238 (MQ=255)

                                           ggTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCCAACAACGATTGCCCCCTCTGATTGGAGGGCAGTTCCGTGACACCGTTAACTTTTTGGCCAA‑AAACAACCGCCAGCCAGCCCAAAAGAAcaccaccacag                                                                                                                                                                                                   >  1:421605/1‑237 (MQ=255)

                                                gTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCAAACGACGACCGTTGGa                                                                                                                                                                                              >  2:184077/1‑239 (MQ=255)

                                                gTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGCCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTACCTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACAACGACCTGTGGa                                                                                                                                                                                              >  2:340662/1‑239 (MQ=255)

                                                gTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGATGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACACCGATTGCACCACCAGATTAGAGTGCAGTTCCGATCCACCGTTAACTTTTGGGCTCA‑AAACAACCGCACACCCGGCCATAACAACAGGACGACCGTGGGa                                                                                                                                                                                              >  1:744322/1‑239 (MQ=255)

                                                gTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGATGAACGCGATGGGTTCGCTACCGCCAGCCAGCGACTCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGACACGACCCGGG‑AAGATTTTCGCAAAATCGGCAAACAACGATTGCACCATCAGACTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCCCA‑CACCACCACGCACCCAGGCCaaccgccccacacgaccggtgac                                                                                                                                                                                              >  1:656795/1‑217 (MQ=255)

                                                 gtccccctttgcgccccGG‑GTGTTGCGCCAGCACGGCTGAAAGCGATGGGTTCGCTCCCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGa                                                                                                                                                                                              <  1:462617/224‑1 (MQ=255)

                                                                  cagtgtttCGTCCCGCACGGGTGAAGGCGATGGGTTCGTTACCGCCAGCCAGCGACGGGGCGTCACACCG‑TTGGGGA‑AGTTGGGGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACg                                                                                                                                                                             <  2:678149/233‑1 (MQ=255)

                                                                   g‑gTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCCGGCCAAACGCACACGACGACCGTTGGAttaccaaatgaccgcccc                                                                                                                                                                            >  1:448159/1‑222 (MQ=255)

                                                                   g‑gTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGACGTTCCGGTACACCGTTAACTTTGTGGCGCA‑CAACAACCGCCAGCCAGGCCATACGCCAACGACGACCGTTgaaacgatcccttaaaaaaaac                                                                                                                                                                           >  1:145922/1‑218 (MQ=255)

                                                                   g‑gTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTACGCAAAGTCGGCAAACAACGATTGCACCCTCAGATTAGAGTGCAGTTCCGATACACCGTTAAATTTGTTGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGCCGCCCGTTGGATTAATCAAGTATCGagcc                                                                                                                                                                            >  1:235380/1‑235 (MQ=255)

                                                                          tgcccgcccgggcgaaaaGCGGTGGGTTCCGCCCCGCCACCCAGCG‑CGCGGGGTCCCCCCGTTTGGTGA‑CGTTGGGGAAACGACCCGGG‑AAGATTTTCGAAAAGTCGGCAAACAACGATTGCCCCATCAGATGAGAGTGCAGTCCCGATACACCGTTACCTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTccc                                                                                                                                                                     <  1:665548/224‑1 (MQ=255)

                                                                          cGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGCCGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTTGGCAAACAACGATTGCACCATCAGATTAGATTGCAGTTCCGATACACCGTTAACTTTGAGGCTCA‑CAAAAACCGCCACCCAGGCCACACGCCCACCAACCACGTTGGATCCTTCAAAAACCGAGACCCGTccc                                                                                                                                                                     >  1:95807/1‑239 (MQ=255)

                                                                          cGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACCACGACCGTTGGATTCATCAATGATCGACGCCCGTccc                                                                                                                                                                     >  2:259463/1‑239 (MQ=255)

                                                                          cGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACCCACACACCCACCGGTGGAATAATAAATGaaccaaccccggccc                                                                                                                                                                     >  1:366199/1‑225 (MQ=255)

                                                                          cGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCAGCAAACAACGATTGCACCCTCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATCCGCACACGACCACCGCTGGATTCATCAATGATCGACGCCCGTcc                                                                                                                                                                      >  1:177907/1‑238 (MQ=255)

                                                                             gcAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGGCGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                                                                                                                                                                   <  2:474274/237‑1 (MQ=255)

                                                                             cccgtccGGGTGAAAGCGGTGGGTTCGCTACCGCAAGCCAGGGACGCGGCCTCCCACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAACCAACGATTGCACCATCAGATTAGATTGCAGTGCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                                                                                                                                                                   <  2:577036/232‑1 (MQ=255)

                                                                             ccAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGGGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                                                                                                                                                                   <  2:26191/238‑1 (MQ=255)

                                                                                gCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGAGGTTCCGATACACCGTTAACTTTGTGGCGCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGCTGGATTACTCACTAATCCACGCCAGTCCcagcag                                                                                                                                                                >  1:26644/1‑238 (MQ=255)

                                                                                 ccggggtgaaggtgtggggTTCCATCCCGCCACCCCGCGGCGTGGCGTCACTCCG‑GTGGTGT‑CGGTGGGGAAACTCCCCGGG‑AATATGTCCGCAAAGTCGGCAACCAACGATTGCACCATCAGATTAGAGTGCAGTTCCGAAACACCGTTAACTTTGGGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGAt                                                                                                                                                              <  1:571721/223‑1 (MQ=255)

                                                                                     gccaaaaGGGGTGGGTTGACTCCCGCTGGCCAGGGACGCGCGTTCACGCTG‑TGGGGGA‑GGTTGGGAACACGACCCGGG‑AAGATTACCGAAAAGTCGGCAAACAACGATTGCACCATCATATTAGGGTGCAGTTCCGTTACGCCGTTAATTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGGCCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGt                                                                                                                                                          <  1:306827/235‑1 (MQ=255)

                                                                                      gtgaagggggtGGGTTCGATCACGCCAGCAGGCGACGGGGGGACACA‑CG‑TTTGTGAGAGTGGGGAAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAACAAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTACCTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGt                                                                                                                                                          <  1:227794/228‑1 (MQ=255)

                                                                                       ctaagggagtggtttcccacccgcccgcCAGGGACGGGGAGTCACCCCG‑TTGGTGA‑ACGTGGGAAAACGACCCGGG‑AAACTTCTCGAAAAGGCGGCAAACAACGATTGCGCCACCAGATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTAt                                                                                                                                                        <  1:12460/219‑1 (MQ=255)

                                                                                        gaagagtgtGGGTTCGGCACCGCCAGCCGGGGAAGGGGATACAGCCCG‑TTGGTGA‑CG‑TGGGGCAAGGACCCGGGAAAGATTGTCGCAAAGTCGCAAAACAACCGTTGCACCAACAGATGAGAGGCAAGTTCCGATACACCGGTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCAGGCGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTAt                                                                                                                                                        <  1:385333/230‑1 (MQ=255)

                                                                                                tGGGTTGGCTACCGCCAGCCAGGGACGGGGCGTCACCCCT‑TTGGTGA‑CGTTGGGGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGa                                                                                                                                                <  2:54346/238‑1 (MQ=255)

                                                                                                tGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CACCAACCGCCAGCCAGGCCATACGCAACCGACGCCCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGa                                                                                                                                                >  2:322793/1‑238 (MQ=255)

                                                                                                tGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCACCAGATCGGTATCGTTCGGAt                                                                                                                                               >  2:177/1‑239 (MQ=255)

                                                                                                tGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTAAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGACCGGTATCGTTCGGAt                                                                                                                                               >  2:173114/1‑239 (MQ=255)

                                                                                                gtgcttCCCTCCCCCCCGCCGGGGACGCGGCGTCACACCG‑TGTGTGA‑CGTGGGTTAAACGCCCCGGG‑AAGATTTTCGCAAAGTCGGCAAAAAACGATTGCCCCATCAGATTAGAGGGCAGTTCCGAAACACCGTTACCTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGa                                                                                                                                                <  1:843584/234‑1 (MQ=255)

                                                                                                            cggcagccgacGACGCGGGGTCACACCG‑TTGGTGA‑CGTTGGGAAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGAAAACAACGATTGCCCCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCa                                                                                                                                   <  2:871148/236‑1 (MQ=255)

                                                                                                            cccccgccaggGGCGGGGGGTCACTCGG‑TTGGTGA‑CGTGGGGAAAACGCCCCGGA‑AAGATTTTCGCAAAGCCGGAAAACAACGATTGCACCTTCAGATTAGGGTGCAGTTCCGATACCCCTTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCa                                                                                                                                   <  1:688834/234‑1 (MQ=255)

                                                                                                                 gccagcGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGtt                                                                                                                              >  2:144677/1‑239 (MQ=255)

                                                                                                                         cggggcgTCACCCCG‑TTGGTGA‑CGTTGGTGAAACGGCCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGa                                                                                                                       <  2:304006/235‑1 (MQ=255)

                                                                                                                                tCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCACCCGAGCGGCGTCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAAAAGtgg                                                                                                               >  1:526244/1‑237 (MQ=255)

                                                                                                                                  cccccG‑TTGGTGA‑CGTTGGAGAAACTACCCGGG‑AATATTTTCGCAAAGTGGGCAAAACTCGATTGGACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAATTTTGTGGCTCA‑CAACAACCGACAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGtt                                                                                                             <  2:235380/236‑1 (MQ=255)

                                                                                                                                     ccG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGAt                                                                                                          <  2:448159/239‑1 (MQ=255)

                                                                                                                                         ttggtga‑cgttggtgaAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGAATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGCTTTc                                                                                                       >  2:148372/1‑239 (MQ=255)

                                                                                                                                         ttggtga‑cgttggtgaAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCACCAATTCGGTATCGTCCGGTAACTGTTCCTGCAAGGTTTTTCGGaaaaaaaggttgtttta                                                                                                       >  1:858996/1‑225 (MQ=255)

                                                                                                                                           ggtga‑cgttggtgaAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATa                                                                                                                                              >  2:886057/1‑198 (MQ=255)

                                                                                                                                           ggtga‑cgttggtgaAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCCCA‑CAACAACCGCCAGCCAGGCCATACGCAAACGACGACCGTTGACTTCATCACTGATCGACGCCCCTCCCACCAGACCGCAATCGTTCGCATCCTTTTCCTGCCAGGTTTCAAGGaataggaggttgatttaaa                                                                                                     >  1:774332/1‑222 (MQ=255)

                                                                                                                                            gttgtgttttgtgaAACGACCCGGG‑AAGTTTTGCGAAACGCGGGCAAAAGAGTATTCCACCACCAGATTAGTGTGCAGTCCCGATGCACCGTTAACTTTTGGGGTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                                     <  1:705836/229‑1 (MQ=255)

                                                                                                                                            ggtgaaggttgagaACCGACCCTGG‑AAATTTTCCGCAAAGGCGACAAACAACGGTTGCCCCATCAAATTAGGTGGCAGTCTCGGACCCCCATTAACTTTGGGGCCCC‑AACCAACCGCCAGCCAGGCCATACGCACCCGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                                     <  1:144677/227‑1 (MQ=255)

                                                                                                                                              ga‑cgttggtgaAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGCGGCCCGTCCCAGCAGAACGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAg                                                                                                                  >  2:881249/1‑223 (MQ=255)

                                                                                                                                               gccgtgggtaaAACGCCCGGGG‑AAATTTGTCCAAAAGGCGGCAAACAACATTTCCACCATCAAATTAGAGTCCAGTTCCGATACCCCGTTAACTTTGGGGCCCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAg                                                                                                   <  1:809567/236‑1 (MQ=255)

                                                                                                                                               a‑cgttggtgaAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGTCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATTGGGATCGTTCGGATCTTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAcat                                                                                                 >  2:544480/1‑239 (MQ=255)

                                                                                                                                                      gtgaAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCATGCAAGGTTTTCAGGAAAAAGACGTTGATTTCAAAGATGATCTg                                                                                           >  2:403516/1‑239 (MQ=255)

                                                                                                                                                               ccgggg‑ATGTTTTTCGCATATTCCGCAAAAAACGGTTCACCCAACAAATTTGGGTGAAGGTCCGGTACACTGTGACCTTTGTGCCCCA‑CACCCACCGCCAGCCAGGCCATACGCACCCGACGAACGGTGGATTCATCAATGATCGCGGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGAcg                                                                                  <  1:814983/236‑1 (MQ=255)

                                                                                                                                                                ccGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CACCAACCGCCAGCCAGGCCATACCCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCAAGCCGATGGGTATCGTTCGGATACTGTTCCTGCCAGGGTTTCCGGAAATAGTCGTTGGATTCAAAGATGATCTCCAGGGGAcgc                                                                                 >  1:301797/1‑239 (MQ=255)

                                                                                                                                                                             tcccAAAGTCGGAAAACATGCACTGCCCCATCAGATTAAAGTGCAGTCCCGATACCCCGTTAACTTTGGGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTa                                                                     <  2:788114/236‑1 (MQ=255)

                                                                                                                                                                                cAAAGTTGGCAAACCACGATTGCACCATCAGATTAGATTGCATTCCCGATCCCCCGTTAACTTTGGGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                                  <  2:307137/239‑1 (MQ=255)

                                                                                                                                                                                cAAAGGCGGCAAACAACGATTGCACCATCAGATTAGAGGGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                                  <  2:70039/239‑1 (MQ=255)

                                                                                                                                                                                 aaaGTCGGAAAACAACGATTGCACCATCAAATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGGGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                                  <  2:526244/238‑1 (MQ=255)

                                                                                                                                                                                      cGGAAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGGGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCat                                                             <  2:366199/238‑1 (MQ=255)

                                                                                                                                                                                          aaaCACAGATTGCACCATCAAATTCAAGTCGAGTTCCGATACACTGTTAATTTTGTGCCCCA‑AAACAACCCACAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCaa                                                        <  2:858996/239‑1 (MQ=255)

                                                                                                                                                                                                  aTTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAg                                                >  2:439830/1‑239 (MQ=255)

                                                                                                                                                                                                            aacttttgggTGCTGTTCCGATCCCCTTTTACTTTTGGGGCCCA‑CAAAACCCGCCAGCCAGGCCATACGCACCCGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAgcg                                      <  1:230036/230‑1 (MQ=255)

                                                                                                                                                                                                              ttgtaaaaagaccacccGATACTCCGTTAACTTTGTGGCTAC‑AAACACCCGCCAGGCAGGCCATACGCCCACGAGGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGcc                                    <  1:159247/224‑1 (MQ=255)

                                                                                                                                                                                                                ttaaagtcgaattCCGATACACCGTTAATTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGcc                                    <  2:787410/226‑1 (MQ=255)

                                                                                                                                                                                                                ttAGAGGGCAGGTCCGAAACCCCGGTAACTTTG‑GGGTCACCAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGcc                                    <  2:415622/237‑1 (MQ=255)

                                                                                                                                                                                                                      tGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCCGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAAATTTACCCAGCAAATCAACCGGCCAGGATTCCAACGCCCCGccaat                            >  1:521704/1‑235 (MQ=255)

                                                                                                                                                                                                                      tGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCACCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGCTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATTTCAACCGGCCAAGGTTCCAGCGCCCCGCtca                             >  1:198368/1‑238 (MQ=255)

                                                                                                                                                                                                                       tcagtctcgagcacccGTTACCTCTCTGCCTCA‑CAACATCCGACCGCCCGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTGTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtcat                            <  2:699925/224‑1 (MQ=255)

                                                                                                                                                                                                                       gttgtcccgtttccccGTTAACTTTGGGTCTCC‑AAACAGCCGCGCGCCAGGCCATACGCACACGACGAGCGTTGGGTTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGGTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtcatc                           <  1:177/225‑1 (MQ=255)

                                                                                                                                                                                                                       cccgtccctaatcccccttttcTTTGTGGCTCA‑AAACACCCGCCAGCCAGGCCCTACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGACGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtcatc                           <  1:340662/218‑1 (MQ=255)

                                                                                                                                                                                                                         aGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCCGGAAATAGTCGTTGATTTCAAAGATGATCTGCA‑GTGACGCGGCAGAATTTTACCCAGCAAATCAACCGGCCAGGTTTCCACCGCCTCGCtccacaac                        >  1:322504/1‑233 (MQ=255)

                                                                                                                                                                                                                         aGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCTTATCAACCGGCCAGGTTTCCAGCGCCCCGCtcactag                         >  2:256610/1‑235 (MQ=255)

                                                                                                                                                                                                                           ttCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCCTACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGCTACTGTTCCTGCAAGGTTTTCAGGAAATAGCCGTTGATTTCAAAGATGATCTCCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCAAGCGCCTTGCCCCTCAGCg                       >  2:366225/1‑239 (MQ=255)

                                                                                                                                                                                                                           ttCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCGGGGCCCCGCccatacccg                       >  1:603726/1‑234 (MQ=255)

                                                                                                                                                                                                                           ttCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGGTTCCAGCGCCTCGCtgctaagcg                       >  2:661855/1‑231 (MQ=255)

                                                                                                                                                                                                                           ttCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGAATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGGGACGCGGCAGAATTTTACCCAGCATATCAACCGCCCCGGTTTCCACCGCCCCCCtcttaaacg                       >  1:320594/1‑232 (MQ=255)

                                                                                                                                                                                                                            tCCGATACCCCGTTAACTTTGGGGATCC‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCgt                      <  2:889512/239‑1 (MQ=255)

                                                                                                                                                                                                                            gccGATGCACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACCCGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCgt                      <  2:392146/238‑1 (MQ=255)

                                                                                                                                                                                                                            cccgaaaaccggTTAACTTTGTGGCCCA‑CAACAACCGCCAGCCCGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCg                       <  2:428472/227‑1 (MQ=255)

                                                                                                                                                                                                                            cccGATCCCCGGTTAACGTGTTGGCTCC‑AAACAACCGCCAGGCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCAGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCgt                      <  2:774332/238‑1 (MQ=255)

                                                                                                                                                                                                                                aTACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATGGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCCCATCAACgggggg                  >  1:604804/1‑234 (MQ=255)

                                                                                                                                                                                                                                 tACCCCGTTAATTTTGTGGCTCA‑CAACAACCGCCAACCAGGCCATACGCACACGACGAGCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                 <  2:198368/239‑1 (MQ=255)

                                                                                                                                                                                                                                 tACCCCGTTAACTTTGGGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                 <  2:320594/239‑1 (MQ=255)

                                                                                                                                                                                                                                 tACACGTTTAACTTTGGGGCCCA‑AAAACACCGCCAGGCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                 <  2:511873/239‑1 (MQ=255)

                                                                                                                                                                                                                                 tACACAGTGAATTTGGGGCCTTA‑CAACAACCGGCAGCCAGGCCATTCGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                 <  2:374489/239‑1 (MQ=255)

                                                                                                                                                                                                                                 cccccttatccttttGTGGTCCA‑CAACAACCCCCACCCCGGCCATAGGCACACGGAGACCGTTTGATTCCACAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGAGACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGCTCTGCAGTTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                 <  1:799039/227‑1 (MQ=255)

                                                                                                                                                                                                                                  cccccGTTAATTTTGTGGCTCA‑CAACAACCGCCAAGCAGGCCATACGCCCACGGCGGCCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                 <  1:68745/235‑1 (MQ=255)

                                                                                                                                                                                                                                    ccctttacttttGGGGCCAA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATGGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAg              <  2:604804/230‑1 (MQ=255)

                                                                                                                                                                                                                                      ctttAACTTTGGGGCCAA‑CAACAACCGCCAGCCAGGCCATTCGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAgtg            <  2:421605/237‑1 (MQ=255)

                                                                                                                                                                                                                                        ttAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtcatca                          >  2:159247/1‑223 (MQ=255)

                                                                                                                                                                                                                                        ttAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGAACCGGCAGAATTTTACCCAGCAAATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGGGTa          >  2:464904/1‑239 (MQ=255)

                                                                                                                                                                                                                                        ttAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGGGACGCGGCAGAATTTTACCCAGCATACCAACCGGCCAGGTTTCCAGTTCCCCGCTCATCAGCGTGTGGTTCGTGTa          >  2:531460/1‑239 (MQ=255)

                                                                                                                                                                                                                                                gtgGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGAATATCAACCGGCAAGGTTTCCAGCGCCCCGCCAATCACCGTGGGGTTAGGGGAGGaaaaaa  >  1:22919/1‑237 (MQ=255)

                                                                                                                                                                                                                                                gtgGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGGGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCCCCCTCATCAGCTTGGGGTAAGTGTAGga       >  2:294031/1‑234 (MQ=255)

                                                                                                                                                                                                                                                gtgGCTCA‑CAACAACCGCCAGCCAGGCCATAAGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTTATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAACCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGTTCATCAACggtgggtttcgtaaggggaag   >  2:157164/1‑218 (MQ=255)

ATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAG‑GTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCG‑TTGGTGA‑CGTTGGTGAAACGACCCGGG‑AAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑CAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGGAGAAGA  >  NC_000913/3565049‑3565523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`