Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,588,515 A→C 17.0% V222G (GTC→GGC)  ugpC ← sn‑glycerol‑3‑phosphate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,588,5150AC17.0% 98.1 / 2.1 48V222G (GTC→GGC) ugpCsn‑glycerol‑3‑phosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (25/14);  new base C (2/7);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 3.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGC  >  NC_000913/3588281‑3588740
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                  
tCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCCGTTCGAAATGCGTGCCTTCGTTATTCCCGCGGCCTGTCAGCAGGTTCATCGCCGGACTTCCGATAAAACCCGCTACAAACAGGCGGGCGGGCTTTTTGTAAACTTc                                                                                                                                                                                                                                >  1:197620/1‑239 (MQ=255)
tCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGGAATCCCGGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTTATCGCCGGACTGCCGATAAAACCCGCCCCAAAAAGGCGGGGGGGCTTTTCCGAAACTTc                                                                                                                                                                                                                                >  1:44085/1‑239 (MQ=255)
tCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGTTAAAACTCGCTACAAACAGCCTGGCGGGCGTTTCCTAGACCTc                                                                                                                                                                                                                                >  2:812800/1‑239 (MQ=255)
     cgccATCGGTACGCCGCCTGCGGCCTGCGAGCTTAGCGAAATAGGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTTTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTg                                                                                                                                                                                                                           <  2:285231/237‑1 (MQ=255)
       cctggggtcctcccccTTTTGGCTGCGGGCGTAGGGAATTAGGTTCCGGGCGAATGCCGAGGGTCATTTTACGCCCGGCAGACTGGCGGTAGCCACCGTTTAGCGGCAGCTCAATGCCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACGGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGgt                                                                                                                                                                                                                         <  1:63355/225‑1 (MQ=255)
          gctgtcgcccccttgttgcctgcGACGTTAGCGTATGTGGTTCCGGGCGAGTGCCGGGAGGCATTTTCCGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGtgtg                                                                                                                                                                                                                      <  1:119867/223‑1 (MQ=255)
               accccgccTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAAt                                                                                                                                                                                                                 <  2:800514/236‑1 (MQ=255)
                  ccgccTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCTTAGACTTCAACtcgatggccaa                                                                                                                                                                                                                  >  2:792021/1‑225 (MQ=255)
                               gcGAGCTTAGCGCAATATGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTGGACTTCAACCGGTGGGCCAATCCGTTCGGCAAAAcc                                                                                                                                                                                                  >  2:563915/1‑238 (MQ=255)
                                       ttctaattgtgtgcgggggaaaTGCCGGGGGTCTTTTTCCCCCCGGCATACTGGCGGTAGCCAGCTTTTAGCGGAAGCACAATCCCGCCGTCCAGTGCGAAATCGTTGCCTTGGTTATTCCCGCGGCCTGTCGGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTtcat                                                                                                                                                                                         <  1:794354/219‑1 (MQ=255)
                                       gcctgagtttGTTCCGCGCCATGGTCGAGGGTTTTTTTACGCCGGGACATCTGGCGGAGGCCCCCGTTTGGCGCACCCCAAAACCCGCCGGCCAGTTCGAAAGGCGGGCCTCCGTTATCACCGGGGTCGGTCGGCAGGTTCACCGCCGGACTGCCGATAAAACCCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTCCCAATCTGTTCGGCAACACCGCCGTtcat                                                                                                                                                                                         <  1:403153/230‑1 (MQ=255)
                                                 gTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCCGTTCGAAATGCGGGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCAACGCCGGACTTCCGATAAAACTCGCAACAAACAGGCGAGCGGGCCTTTCCGACACTTCAACACGTGGTCCAATATATTCGGGCAAACCCCCGTCCACCACCATCAc                                                                                                                                                                                >  1:110362/1‑238 (MQ=255)
                                                      gggCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTTTCAGCAGGTTCATCGCCGGACTGCCGATAAACATCGCTCACACCAGTCTGGCAGGCTTTTCGTCGACTTCAACTCGGGTGCCAACCTGTTTGGCCACCACTCCCGTCCTCACCAtaaacccggt                                                                                                                                                                          >  1:150735/1‑230 (MQ=255)
                                                        gCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGAc                                                                                                                                                                                                                                   <  2:569124/180‑1 (MQ=255)
                                                           aaTGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAAACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCCCCGTTCATAACCATTACTCGCTGGGCGa                                                                                                                                                                     >  2:681255/1‑239 (MQ=255)
                                                                       aaTTTTACGCCCGGCATACTGACGGTAGCCACCGTTTGGCGGCAGCTCAATCCCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTAAGCAGGTTCATCGCCGGACTGCCGATAAACCTCGCTACAAACAGGCTGGGGGGCTTTTCGTAGCCTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCtt                                                                                                                                                         <  2:783982/238‑1 (MQ=255)
                                                                            tccccacGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGGGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGCCTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAAcc                                                                                                                                                    <  2:791805/233‑1 (MQ=255)
                                                                              cGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCCACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCCCTGGGCGAGTGTCCTCGCTTCAACCtt                                                                                                                                                  >  2:447913/1‑238 (MQ=255)
                                                                                  ccggattACGGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGTTTCATCGCCGGACTGCCGATAAATCTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGCCTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCg                                                                                                                                                                             <  2:116092/203‑1 (MQ=255)
                                                                                   ggCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTACACTGGGGGGCCAATCTTTTCGCCAAAACCACCCGTCATCACCCTTACCCCCTGGGCGAggcgcacccctcacacccgtactg                                                                                                                                             >  1:372089/1‑216 (MQ=255)
                                                                                    ggaaaccggcgggTGGCCCCCGTTTAGGGGCAGCTCAATCCCGCCGCCCGGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTAATCGCGGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGt                                                                                                                                             <  2:884161/228‑1 (MQ=255)
                                                                                                       cccgtttggggggcccacatccccgccgCCCGGTTCAAAAGGGGGGCCTGCGTTATCCACGGGGCCTGTCGCCAGGTTCATCGCCGGCCGGCCGATAAAACTCGCTACAAGCGGGCTGGCGGGCTTTTCGTAGCCTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAg                                                                                                                           <  1:21615/217‑1 (MQ=255)
                                                                                                         gTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTTGTAGCCTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGTCTGCCGTCATCGCTTCCACCTGATCGTGCGCAACGTCGAGGGAAGACg                                                                                                                        >  2:338398/1‑239 (MQ=255)
                                                                                                                  cAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGGGAAGTGCGTTTCAGGc                                                                                                               >  2:6246/1‑239 (MQ=255)
                                                                                                                    gCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGGGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTAATCGCTGGGCGATGGTCCTCGCTTCAACCTGATCGTGAGAAACGAAAAGTGAAGTCGTTTTCAGGGGa                                                                                                             >  1:727037/1‑239 (MQ=255)
                                                                                                                        aaTCCCGCCGTCCAGTTCAAAAGGCGGGCCTTCGTTATTCCGGGGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGt                                                                                                         <  2:197620/239‑1 (MQ=255)
                                                                                                                              cgcctTCCATTTCAAAAGGCTGGCCTTCGTTATTCCCGGGGCCTGTCAGCAGTTTCACCGCCGGCCTGCCGATAAACCTCGCTACAAACAGGCTGGCGGGCTTTTCGAAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAAc                                                                                                    <  2:435992/239‑1 (MQ=255)
                                                                                                                                ccgTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTTAGGCGCCGGTGCa                                                                                                      >  2:143264/1‑235 (MQ=255)
                                                                                                                                ccgTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGGAGCCTTCAAATGGTGGGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGGGGGGCATCCCTTCAACCTGATCGTGAGTAACGTAGAGGGGAGGCGTTTTCAGGCGCCgggccaaagg                                                                                                  >  1:726720/1‑231 (MQ=255)
                                                                                                                                   tCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGGGGGAAGTCGTTTTCAGGCGACGGTGCaaacgtt                                                                                                >  2:442713/1‑233 (MQ=255)
                                                                                                                                     cAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACAGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATc                                                                                                                                               >  2:206542/1‑189 (MQ=255)
                                                                                                                                             aaTGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGGAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAg                                                                                      >  2:857325/1‑238 (MQ=255)
                                                                                                                                                                cACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGGGAAGTCGTTTTCAGGCGACGGTGCACCTGTTGCAGTTCAATACGAATCTGCCCGGCCAGCt                                                                 >  2:857655/1‑239 (MQ=255)
                                                                                                                                                                   gcgGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGCGCAATCTGCACGCGCGGCCTgg                                                              >  2:452166/1‑239 (MQ=255)
                                                                                                                                                                      cccTGTCGCCGGGTTCATCCGCGGCTCGCCGAAAAACCCCGCTAAAAACAGGCGGGGGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCGTTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCAt                                                           <  1:491364/238‑1 (MQ=255)
                                                                                                                                                                       ccTGTCAGCAGGTTCCTCCCCGGACTGCCGATAAACCTCGCTACAAACGGGCTGGCGGGCTTTTCGTAGCCTTCAACTGGTGTGCCAATCTGTTCGGCAACCCCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCAt                                                           <  2:643615/238‑1 (MQ=255)
                                                                                                                                                                        taaagaggcgttttggcGCCGTGCTGCCGAAAAACCTCGCTAAAAACCGGGTGGGGGGCTTTTCGTAGCCTTCAACTGGTGTGCCAATCTGTTCGGCACCCCCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCg                                                         <  1:140781/223‑1 (MQ=255)
                                                                                                                                                                            cagcagGTTCAGCGCCGGACTGCCGATAAAACTCGCTAAAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAgg                                                      <  2:815306/238‑1 (MQ=255)
                                                                                                                                                                                 ggTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTagag                                                >  2:15707/1‑239 (MQ=255)
                                                                                                                                                                                   ttCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGGGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGGGCAACTGTTGCAGTTCAAAACGCCTCTGCACGCGGAGCTTGGCACCGAGGTTagagag                                              >  2:824633/1‑239 (MQ=255)
                                                                                                                                                                                     cATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGGGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCCCGCCCAGCTTGGCATCGAGGTTCGAGAACg                                            >  2:721315/1‑239 (MQ=255)
                                                                                                                                                                                           cgcactgcactaaaatcccGCTACAAACGGGCTGGCGGGTTTTTCGTAACCTTAAACTGGTGGTCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGGGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCtcat                                      <  1:15707/221‑1 (MQ=255)
                                                                                                                                                                                             gACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTTGCATCGAGGTTATCAACCGGCccatca                                    >  2:141365/1‑239 (MQ=255)
                                                                                                                                                                                             gACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTagaga                                               >  2:140781/1‑228 (MQ=255)
                                                                                                                                                                                              aCTGCCGATAAAACTGGCTACAAACAGGTTGGCGGGTTTTTCGTAGACTTCAACTGGTGTCCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCtcatca                                    <  2:580394/238‑1 (MQ=255)
                                                                                                                                                                                                                          cTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGTATCGCGCCCaa        >  2:208939/1‑238 (MQ=255)
                                                                                                                                                                                                                            ggCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTaga                                                 >  2:732244/1‑195 (MQ=255)
                                                                                                                                                                                                                                ggtttttCGTCAACTTAAACTGTTGTCCAAACTTGTCCGGCAACCCCCCCGGTCATCACCATTACCCGGTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATcgcgc  <  2:498270/235‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                  
TCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCGAGAGTCATTTTACGCCCGGCATACTGACGGTAGCCACCGTTTAGCGGCAGCTCAATACCGCCGTCCAGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGC  >  NC_000913/3588281‑3588740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: