Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,680,184 | T→G | 18.7% | V256G (GTG→GGG) | kdgK → | 2‑dehydro‑3‑deoxygluconokinase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,680,184 | 0 | T | G | 18.7% | 115.4 / 2.5 | 77 | V256G (GTG→GGG) | kdgK | 2‑dehydro‑3‑deoxygluconokinase |
Reads supporting (aligned to +/- strand): ref base T (22/35); new base G (14/4); total (38/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.00e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAGAGACACAGCAGGTGTACCAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCATCCATAAATA > NC_000913/3679949‑3680408 | ttttaaggagcaggggTTTACCAACAAATGCTGGAATTCCGGGATATCCCCTTCCGGGCGCCGGACGGCGAAGACTCGGTGTGG‑GGTAAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGAt < 1:427771/225‑1 (MQ=255) acacAGCAGGTGTACCAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGGGTCCATTGCTGGCGAAGGGTTAGTGGAGGTTCCGGCGGGGAAAATGCCGAAAGAAAAAGGGAACGGTa > 2:342957/1‑238 (MQ=255) gcagGTGTACCAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGAGGGTCCGCGGGGGGAACAGCCGAAAAAAAAAAAGATAGAAACAACCg > 1:270729/1‑239 (MQ=255) gtACCAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGGTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGGGATGGATcccccccaagcgc > 2:315447/1‑232 (MQ=255) tACCAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCCATACCACCGCAGccg > 2:693907/1‑236 (MQ=255) ccAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTTCCGAAAGAAAAAGGGATCGATACACCCGCAGCTGGc > 2:457368/1‑238 (MQ=255) ccAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGGGATCGGTACCCCCCCAGCTggg > 2:147681/1‑237 (MQ=255) ccAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGCTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGGAAAAGGGATCGATAACACCGCAGCTGGc > 2:662398/1‑238 (MQ=255) gcggaTGGTGGAAGGCCCGGATATCGCCCTCCTGACGGCGGACGACGAAGGCGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGAc < 2:804053/234‑1 (MQ=255) acaaATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGAct < 2:209996/239‑1 (MQ=255) caaATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACTCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGCAAGAAAAAGGGATCGATACCACCGcggtctcttataa > 2:427771/1‑227 (MQ=255) ggAATGCAGGGAGTACGCCTTCCTGACGCTGGACGACGAAGACGCG‑TGTGGCGGTCAACAGGCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGt < 2:467238/238‑1 (MQ=255) gAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGCGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGCGAAGGAAGTGGTGGTGAAACCCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGGAACTGCCGAAAGAAAAAAAGATCGAAACCACcacacccggggcatatttcaggtg > 1:848906/1‑219 (MQ=255) cACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGGGAACCTGTCGGAAGAAAAAGGGAGCGGAACAACCGCCGCCGGGGCCTCTTTCCGTGcccgc > 1:804053/1‑235 (MQ=255) ccccTTCCTGCCCGTGGGCGAGGAAGGCGGGCTGGGG‑GGTCAACAGCGGGGGGAAGACGGCATTGCGCGCACCCATAACGGGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCgg < 1:457368/237‑1 (MQ=255) tCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGGAAAAGTGATAGATCACCCCGCAGCCGGCGCCTCTTTCAGTGCCGGGTATCTGGCGGTACGt > 2:669136/1‑239 (MQ=255) ccTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGGGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACg > 2:710592/1‑237 (MQ=255) ccTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAAATGCCGAAAGAAAAAGTGATAGCTACCACCGCAGCTGGGGACTCTTTCAGGGCCGGTTATCTGGCGGTACg > 2:497650/1‑237 (MQ=255) ccTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGGTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGGGATCGATACCACCGCAACTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACg > 2:341986/1‑237 (MQ=255) ggACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCCCAGCTGGGGACCCCTTCAGTGCCGGGTATCTGGCgggcagtctggaagggg > 1:242957/1‑224 (MQ=255) gacgacGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGGGAAACTGCCGCAAGAAAAAGGGATCGATACCACCGCAGCTGGCGAATCTTTCAGTGCCGGGTATCTGGCGGTACGTCTGACAggcgg > 2:74914/1‑239 (MQ=255) cggagtccgcgtgggg‑ggTCACGAGCCGGGGGAAGCAGTACTTTCGCGCCCCAATAACGCGGGCGTGAAAGAAGTGGTGGGGAAACGGGGGGCGGATTCTTGCCTGGTGTCCATTGATGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCa < 1:315447/222‑1 (MQ=255) cgaAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAACGAAGTGGTGGTGCAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGAGGTTCCGGCGGTGAAACTGCCCAAATAAAAAAGGACCGCTAACCCCGCCACTGGGGAACTTTTCACGGCCCGTTATATGGCGGGACGTCAGAAAGGCGGCAggc > 1:735329/1‑237 (MQ=255) aCGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACCCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGaa > 2:690712/1‑239 (MQ=255) ggTGGAAGAGGTCATTGCGCGCACCCATAACGGGGGGGTGAAAGAAGTGGGGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCAcc < 1:113494/239‑1 (MQ=255) atttGCGCGCCCCCATAACCGGGGCGTGAAAGAAGGGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGGCCATTGCTGGCGAAGGGTTAGTGGATGGTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGAATCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTa < 1:742985/237‑1 (MQ=255) aTTGCGGGAACCCAAAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTa < 2:225313/238‑1 (MQ=255) ttgggccACCCATAACGGGGGTGTGAAAGGAGTGGGGGTGAACCGCGGGGCGGATTCTCGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTTCCGAAAGAAAAAGTGAGCGATTCCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCg < 1:154836/233‑1 (MQ=255) tGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGGCAAGCGGCAGGGCGGCAGACGCGGG‑CGAACGTGGGCACCAGACCGCCAGGACCg > 2:652855/1‑239 (MQ=255) cgcACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGGGGGCCCCTGACCGCAAGTACCGTTAt > 2:571618/1‑239 (MQ=255) cgcACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGGGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGGTAGCTGGCGGTACGTCTGACCGGCGGCAGCGCGGAAGACGCGGCGGAAACGGGGGccccggccacaagaaacggtat > 1:448977/1‑221 (MQ=255) cgcACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGGGATCGATACCACCGCACCTGGCGCCCCTTTCAGTGCCGGTTATCTGGCGGTCAGTCTGACAGGCGGGCGCGCGGGAGAGACGGG‑GGAACATgggggcccgccccccaggacgcgttt > 1:215101/1‑216 (MQ=255) aCCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGGACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GGAACGTGGGCACCTGACCGCAAGGACCGTTATTCa > 2:459704/1‑239 (MQ=255) cagcggggCGTGAAAGAAGTGTGGGGGAAACGGGGGGGGGATTCTTGCCTGGGGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATcg < 1:264097/234‑1 (MQ=255) ggCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGCACGCAAGAACCGCTATTCAGTATAGCGgggc > 2:328991/1‑239 (MQ=255) ggCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGAAGCGGC‑GAAACGGGGGCACCTGGACGCAAGGCCCGGTATTCAGTATCgcgggcc > 2:153055/1‑236 (MQ=255) ggCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCCCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTAtatgtcgct > 2:323841/1‑231 (MQ=255) cGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCCCGGGAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTTTTCAGTATCGCGGcgcgg > 2:603804/1‑238 (MQ=255) aagaagGGGTGGTGAAACGGGGGGCGGTTTCTTGCCTGGTGGCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATc < 1:571618/239‑1 (MQ=255) ggtggggAACCCGGGGTCGGATTCTTGCCTGGTTTCCATTGCTGGCGAAGGGTTAGTGGATGTTCGGGCTGTGAACCTGCCGAAAGAAAAAGTGATCGACACCCCCGCAGCTGGCGACTCTTTCAGTGCCGTTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGt < 1:512314/238‑1 (MQ=255) gggggggAAACGCGGGGGGGATTTGTGCCTGGGGTCCATTGCTGGCGAAGGGGTAGTGGATGGTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATCCCACCGCAGCTGGCGACTCTTTCAGGGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGt < 1:497650/232‑1 (MQ=255) gtggtgAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGGGATCGATACCCCCGCAGCTGGCGACTCTTTCAGTGCCGGTTTTCTGGCGGTACGTCTGGCAGGCGGCAGCGCCGGAGACGCGGC‑GAAACGGGGGCACCCGACCGCAAGAACCCGTATTCAGTATCGCGGGGCGACTAACCcccgggg > 1:156667/1‑236 (MQ=255) tgAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGG‑GAAACGGGGGCACCTGACCGCCAGGACCGTTATTCAGTATCTCGGCGCGTTTTTCCCCCCTGAgggg > 2:483957/1‑237 (MQ=255) aCGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTTTCTGGCGGTACGTCTGGCAAGCGGGAGCGCGGAAGCCGCGGC‑GGAACGCGGGCACCTGACCCCCAGTACCGTTATTCACTATCGCGCGGCGGGTATCCcgccgggggggacga > 1:607734/1‑227 (MQ=255) tttttgccgggttctcATTGCTGGCGAAGGGTTAGTGGAGTTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACg < 1:553906/223‑1 (MQ=255) ttGCCTGGGGCCCATTGCTGGGGAAGGGTTAGGTGATGTTCCGGCGGTTAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGGCTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑AAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACgcagc < 2:898020/239‑1 (MQ=255) gCCTGGTGTCCATTGGTGGCGAAGGGTTAGTTGATGTCTCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCat < 2:218521/239‑1 (MQ=255) ggTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GGAACGTGGGCACCCGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACgaagccatagc > 2:526124/1‑233 (MQ=255) tgtCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGGAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGACGACGCGGC‑GAAACGTGGGCCCCTGACCGCAAGTACCGTTATTCAGGATCGCGGCGCGGTTATCCCGCGGGAGGGGATGCCCCCGGAAACGCAGCAATTGCaa > 1:871416/1‑239 (MQ=255) tgtCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGGGAGGCGAGGCCCGCGTAAACGCAGCATATGCaa > 2:447822/1‑239 (MQ=255) tgtCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGAGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGGGAGGCGATGCCAGCGGAAACGCAGCCTATGCaa > 2:584449/1‑239 (MQ=255) tgcccATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATCCCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCa < 2:512245/235‑1 (MQ=255) gtgccATTGCTGGGGAAGGGTTAGTGGATGTTCCGGTGGTTAACATGCCGAAAGTAAAAGTGATCGATACCTCCGCAGCGGGCGACTCTGGCAGTGGCGGTTATCGGGCGGGACGTCTGACCGGCGGCGGCGCGGAGGACGGGGC‑GAAACGTGGGCACCTGGCCGCAAGTGCCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGCCGATGCGAGCGTAAACGCAGCATATGCa < 1:802771/235‑1 (MQ=255) cATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAgg < 2:156667/239‑1 (MQ=255) cATTGCTGGCGAAGGGTTAGTGGATGGTCCGGCGGTTAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAgg < 2:435274/239‑1 (MQ=255) aatGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGc < 2:29159/237‑1 (MQ=255) aTTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGc < 2:213169/239‑1 (MQ=255) aTTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGc < 2:197797/239‑1 (MQ=255) aTTGCGGGCGAAGGGTTAGTGGATGTTCCGGGGGTGAAACTGCCGAAAGAAAAAGTGACGGATGCCACCGGAGCGGGCGACTCTTTCAGGGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGc < 1:74914/239‑1 (MQ=255) tggaaaggtttattgcgttttCCGGGGGTGAAACGGCCGAAAGAAAGAGTGGTCGATCCCCCCGGAGGTGGCGACTCATTCAGGTGCGGTGAACTGGCGGGACGGCTGACAGGCAGCAGCGCGGAAGACGCGGA‑GAAGCGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATa < 1:125788/220‑1 (MQ=255) gCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTCTTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGGTaa > 2:127134/1‑239 (MQ=255) gCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATa < 2:263130/238‑1 (MQ=255) ttAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTa < 2:463652/238‑1 (MQ=255) ggATTTTCCGGTGGTGAAACTGCCGAAAGAAAAAGGTGTCGAAACCGCCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCct < 2:282812/238‑1 (MQ=255) ggATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCct < 2:848906/238‑1 (MQ=255) tGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTAAGTCTGACAGGCGGCCGCGCGGAAGACGCGGC‑GGAACGTGGGCACCTGACCGCACGTACCGGTATTCAGTATCGCGGCCCGATTATCCCCCGTGAGGGGGGGCCCACGTAAAAGCAGCCCATGCAAAAGGCTggggaaaagtttaacccccca > 1:131664/1‑219 (MQ=255) ttCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACCGGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGGGAGGCGATTCCAGCGTAAACGCCGCATATGCAAAAGGCCGGATAAAGTGTTACCCCTCTATcc > 2:456919/1‑239 (MQ=255) ggcggTGAAACTGCCGAAAGAAAAAGGGACCGATCCCCCCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCACAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCt < 1:710592/239‑1 (MQ=255) cggTCAAACTCTCGAAAGAAAAACTGTTGGTTTCCCCCGCCGTCGGCGGCTCTTTCAGTGCCGGTTATCTGGGGGGACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCa < 1:338828/239‑1 (MQ=255) agTGAAACTGCCGAAAAAAAAAGGGATCGATACCACCGAAGCTGGCGACTCTTTCCGTGCCGGTTATCTGGCGGTACGTCTGGCAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCCCCTGGCCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCa < 2:567856/237‑1 (MQ=255) gTGAAACTGGCGAAAGAAAAAGTGATCGATTCCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCAt < 2:841695/238‑1 (MQ=255) gTGAAACTGCCGAAAGAAAAAGTGATCGATACCCCCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCAt < 2:871416/238‑1 (MQ=255) gTGAAACGGCCGAAAGAAAAAGTTATCGAGACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCAt < 2:604029/238‑1 (MQ=255) aCTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTGCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCATCCATaa < 2:607734/239‑1 (MQ=255) cTGCCGAAAGAAAAATGGACCGATCCCCCCGCAGGTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCATCCATaaa < 1:526124/239‑1 (MQ=255) cTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCATCCATaaa < 2:131664/239‑1 (MQ=255) cGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGGGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGAGTTACACCTCTATCCAGCCCATCCATAAATa > 2:206506/1‑237 (MQ=255) | GAAGAGACACAGCAGGTGTACCAACAAATGCTGGAATGCACGGATATCGCCTTCCTGACGCTGGACGACGAAGACGCGCTGTGG‑GGTCAACAGCCGGTGGAAGACGTCATTGCGCGCACCCATAACGCGGGCGTGAAAGAAGTGGTGGTGAAACGCGGGGCGGATTCTTGCCTGGTGTCCATTGCTGGCGAAGGGTTAGTGGATGTTCCGGCGGTGAAACTGCCGAAAGAAAAAGTGATCGATACCACCGCAGCTGGCGACTCTTTCAGTGCCGGTTATCTGGCGGTACGTCTGACAGGCGGCAGCGCGGAAGACGCGGC‑GAAACGTGGGCACCTGACCGCAAGTACCGTTATTCAGTATCGCGGCGCGATTATCCCGCGTGAGGCGATGCCAGCGTAAACGCAGCATATGCAAAAGGCTGGATAAAGTGTTACCCCTCTATCCAGCTCATCCATAAATA > NC_000913/3679949‑3680408 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |