Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 194,997 C→A 11.1% A32E (GCA→GAA)  ispU → undecaprenyl pyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913194,9970CA11.1% 277.1 / 2.6 119A32E (GCA→GAA) ispUundecaprenyl pyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (59/42);  new base A (2/13);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 1.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

ATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTAT  >  NC_000913/194772‑195231
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
ataGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGCTGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCCTGGCTGCCGGCATGTTCCATTCCTTATGGACGGCAAAGGCCGCTGGGCAAAAAAACAAagg                                                                                                                                                                                                                                 >  1:331322/1‑239 (MQ=255)
ataGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTTCGACCATTATGGACGGCAATGGCCGCTGGGCAAAAAAACACggg                                                                                                                                                                                                                                 >  1:844603/1‑239 (MQ=255)
 tatgtttccgcatgtgtttgTAAGGAGTTGGTTTTATAAGGTTAAATATGCCGTGGTTTTACCCGGCTTTTTTTTGTATAGGCGTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGa                                                                                                                                                                                                                                <  1:598182/220‑1 (MQ=255)
 taGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGAAAGGGa                                                                                                                                                                                                                                >  2:30253/1‑239 (MQ=255)
      tGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTATCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGACCATTATGGACGGCAATGGCCCCTGGGCAAAAAAGCAAGGGAAAAta                                                                                                                                                                                                                           >  1:147634/1‑238 (MQ=255)
      tGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGtt                                                                                                                                                                                                                           >  2:514405/1‑239 (MQ=255)
        cgcCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGTAAAATTGCCAGACCATGGTTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGTGCAAAAAAGCCACGGAAGCTTCg                                                                                                                                                                                                                         >  2:586914/1‑239 (MQ=255)
        cgcCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCg                                                                                                                                                                                                                         >  2:628412/1‑239 (MQ=255)
             tCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCGGCGCATGGCTGCCGCCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGAAAAAAAGAAAGGGAAAATTCGGGCCt                                                                                                                                                                                                                    >  1:264287/1‑239 (MQ=255)
              cTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTAAACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCtt                                                                                                                                                                                                                   <  2:331322/239‑1 (MQ=255)
               tgagtgTAAGTATTTGGCTTTATAAGGTCAGATAGGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCttt                                                                                                                                                                                                                  <  2:851659/234‑1 (MQ=255)
                       agtagtTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCAAGCGCATGGGTGCCGTCATGTTGCGCTCATTATGGACGGCAATGGCCGCTGGGAACAAAAGAAAGGGAAGATTCTTGCCTTTTGGCATaa                                                                                                                                                                                                          >  1:152586/1‑239 (MQ=255)
                            ttGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCg                                                                                                                                                                                                    >  2:230533/1‑239 (MQ=255)
                            ttGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAAATTCGTGCCTTTGGGCAAAAAACCg                                                                                                                                                                                                    >  1:113059/1‑239 (MQ=255)
                              ggCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGGGCCTTTGGGCATAAAGCCggg                                                                                                                                                                                                 >  2:857809/1‑239 (MQ=255)
                                cTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTCCCACCGCATGGCTGCCGTCATTTTGCGATCATTATGGACGGCAATGGCCGCTAGGCAAAAAAGAAAGGGAAGATTAGTGCCCTTGGGCATAAAACCcggga                                                                                                                                                                                               >  1:615949/1‑238 (MQ=255)
                                   tataAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAACCGCGTGAGGTTGTCTGCTACTCACCCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGACGTGTTGCGACCCTAATGGACGGCAATGGCACACTGGCAAAAAAGCATGGGAAAATCCGGGCCTTTGGACCTAAAGCCGGGGCaaa                                                                                                                                                                                            >  1:467339/1‑239 (MQ=255)
                                          tCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGGGCCTTAGGGCATAAAGCCGGGGCAAAAaccgta                                                                                                                                                                                     >  1:137936/1‑238 (MQ=255)
                                           cAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGTGGCCAAAtccgtc                                                                                                                                                                                     >  2:539908/1‑238 (MQ=255)
                                              atatGCCGTGGTTGTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGGAAAATTGCCAGCGCATGGCTGCCGTCATCTTGCGATCATTATGGACGGCAAAGGCGCCTGTGCACAAAAGCAAGGGAAGATTCGGGCCTTTGGGAATAAATCCGAGGCCAAACCAGTacgcc                                                                                                                                                                                 >  1:224213/1‑239 (MQ=255)
                                                  gcctgtgTTTTACACGGTTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAACCCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGc                                                                                                                                                                             <  1:514405/233‑1 (MQ=255)
                                                   ccGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTTCGATCATTATGGACGGCAATGGCCGCGGGGCACAAAAGCAAGGGAAGATTCGTGCCCTGTGGCATAAAACCCGGCAAAAAACAGCCCGCCgg                                                                                                                                                                               >  1:36861/1‑236 (MQ=255)
                                                       ttttttACACGGTTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGtc                                                                                                                                                                         <  1:9829/236‑1 (MQ=255)
                                                        gTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTgcc                                                                                                                                                                         >  2:440192/1‑235 (MQ=255)
                                                        gTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCAGTCCGCCGGGCTGtc                                                                                                                                                                         >  2:762918/1‑237 (MQ=255)
                                                            tccacgctttttttttGTTTAGGCTTCAGTTTTCCGGTGTTCCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTAGGCTACTCACCCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTAGGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCtttt                                                                                                                                                                   <  1:439763/231‑1 (MQ=255)
                                                             acacGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTg                                                                                                                                                                  <  2:351879/239‑1 (MQ=255)
                                                                  gCTTTTTTTTGGATGGGCTTCAGTTTTTCCGGATCCCGTAAACCCTGGCAGGGAATAAAAAACGCGGGATGTTGTCTGCCACTCAACCAATTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGcc                                                                                                                                                             <  1:868149/239‑1 (MQ=255)
                                                                   cTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCca                                                                                                                                                            >  2:424283/1‑239 (MQ=255)
                                                                   cTTTTTTTTGTATAGGCTTCAGTATGCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGcc                                                                                                                                                             <  2:264287/238‑1 (MQ=255)
                                                                       tttttGTTTTGGCTTCAGTTTTCCTGAGTACCGTAAACCCTGTAAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaacaa                                                                                                                                                        <  1:230533/239‑1 (MQ=255)
                                                                       tttttGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaacaa                                                                                                                                                        <  2:41362/239‑1 (MQ=255)
                                                                       ttgttGTATAGGTTACAGTATTCCTGAGTACCGTAACCCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaacaa                                                                                                                                                        <  2:543415/236‑1 (MQ=255)
                                                                             tataGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCTTAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCCCTTTTCCGCTCAACAACGGTAt                                                                                                                                                  >  2:729812/1‑239 (MQ=255)
                                                                              ataGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTAtt                                                                                                                                                 <  2:113059/239‑1 (MQ=255)
                                                                              ataGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTAtt                                                                                                                                                 <  2:741177/239‑1 (MQ=255)
                                                                                agttttCAGTATTCCTTGGTACCGAAAACCCTGACAGGGAAAAAAAAAGGGTGGATGTTGCCTGCTGTCCAACCAATTAGCGAAAAATTGCCAGCCCATGGCTGCCGTCATGTTTCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGa                                                                                                                                               <  1:825050/235‑1 (MQ=255)
                                                                                aGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCGGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGa                                                                                                                                               <  2:152586/239‑1 (MQ=255)
                                                                                     tcatgttttctaggaaCCGTAACCCCAGGCAGGGAATAAAAACGCCGGGATTTTTGCTGATACTCAACCGCTTAGGGAAAAATGCCCAGCGCATGGCTGCCGACATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAGCCAGGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTTTTTTGCTGCCAACAACGGTATTGAGGCGt                                                                                                                                          <  1:31355/224‑1 (MQ=255)
                                                                                      cAGTTTTCCTGGGTCCCTGAACCCCTGTGAGGGAATAAAAAACGCGTGAGTTTGTCTGCTACTCAACCACTTAGCGAAAAATGGCCAGCGCATGGCGGCCGTCATGTTGCGATCATTAGGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGtt                                                                                                                                         <  1:412807/239‑1 (MQ=255)
                                                                                       aGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCGTAAAACCGGGGCAAAAACCGTCCGCCGGGCTGTCCCCTTTGCGGCCAACAACGGAATTAAGGCGtt                                                                                                                                         >  1:563326/1‑238 (MQ=255)
                                                                                       aGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACACCGGGATTGAGGCGTTa                                                                                                                                        >  2:29851/1‑239 (MQ=255)
                                                                                       aGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCAGGGCTGTCCCTTTTGCGGCCAACAACGGTATTGCGGCGTTa                                                                                                                                        >  2:661888/1‑239 (MQ=255)
                                                                                         tATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCg                                                                                                                                           <  2:255266/234‑1 (MQ=255)
                                                                                          aTTCCAGAGTACCGTAAACCCTGTAAGGGAATAAAAAACCGGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACg                                                                                                                                     <  2:147634/239‑1 (MQ=255)
                                                                                             ccTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGTCAATGGCCCCTGGGCAAAAAAGCAAGGGAAGATTCGGGCCTTTGGGACAAAACCCGGGGCAAAATCCGTCCGCCGGCCGGTCTCTTTTCGGGCAAACAACGATATTGAgggtgtacacgtg                                                                                                                                  >  1:746738/1‑228 (MQ=255)
                                                                                                 aGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCACAAACGGTATTGAGGCGTTAACCCTGTAt                                                                                                                               >  2:711779/1‑238 (MQ=255)
                                                                                                 aGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTAt                                                                                                                               >  2:617424/1‑238 (MQ=255)
                                                                                                   tACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCACGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGGGGCCCACAAAGGTATTGAGGGGTTAACGCTgaaacg                                                                                                                             >  1:255266/1‑233 (MQ=255)
                                                                                                   tACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTTTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATg                                                                                                                              >  2:245461/1‑237 (MQ=255)
                                                                                                        tAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAAAAACGGGATTTGAGCGTTAACGCTGTATGCCTTtag                                                                                                                       >  1:184699/1‑239 (MQ=255)
                                                                                                        tAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAACAAGGGAAGATTCGGGCCTTTGGGCATAAAGCCTGGGCAAAATCCGTCCCACGGGATGGCTCTTTTGCGGCAAACCACGGGATTTATGGGTTAACCGCGTATGACttttg                                                                                                                       >  1:184978/1‑237 (MQ=255)
                                                                                                           aCCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTtagta                                                                                                                     >  2:712830/1‑239 (MQ=255)
                                                                                                             ccTTTCAGGGAATAAAAAACGCGTGTGTTTGTCTGCTACTCAACCCCTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTg                                                                                                                  <  1:628412/239‑1 (MQ=255)
                                                                                                             ccTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTg                                                                                                                  >  2:242383/1‑239 (MQ=255)
                                                                                                             ccTGTAAGGGAATAAAAACCGGTTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCAGGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGAAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTg                                                                                                                  <  1:539908/239‑1 (MQ=255)
                                                                                                               tGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGTGATCATTTTGGACGGCAATGGCCGCTGGGCAGAAAAGCAAGGGCAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAACCCGTCCGCCGGGCTGTATCTTTTGCGGCCAACAACGGAATTGTGGGGTTAACGCTGTATGCCTTTGGTAGGGaa                                                                                                                >  1:884336/1‑239 (MQ=255)
                                                                                                               tGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGaa                                                                                                                >  2:621384/1‑239 (MQ=255)
                                                                                                               tGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCGTTATGCCTTTAGTAGTGaa                                                                                                                >  2:762425/1‑239 (MQ=255)
                                                                                                                gTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGaaa                                                                                                               >  2:105436/1‑239 (MQ=255)
                                                                                                                            aaaacccgtgtggtgttttccTACGCAACCACTTAGCGAAAAATTGCCGCCGCAGGGCTGCCGTTATGTGGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAGGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAGCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGAc                                                                                                   <  1:661888/219‑1 (MQ=255)
                                                                                                                               ccggtgttttttgtaagCTCCTCAACCACTTAGGGAAAAGTTGCCGTGTAGTGGGGGCCTTCATGTTCCGTTAATTATGGACGGAAGTGGCCGTTGGGGAAAAAGGCAGGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGAAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAg                                                                                                <  1:729812/223‑1 (MQ=255)
                                                                                                                               aCGCGTGATTTTGTCTGCTTCTCAACCGCTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAg                                                                                                <  2:91982/239‑1 (MQ=255)
                                                                                                                                 gcgTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCGGTCTCTTTTGCGGCCAACAACGGTATTGGGGCGTTAACGCTGTTGGCCTTTAGTAGGGAAACATGGAACCgcacacg                                                                                               >  1:91982/1‑232 (MQ=255)
                                                                                                                                           ggcTGCTACTCAACCAGTTAGCGAAAAATGGCCAGCGCATGGCTGCGGTCATGTTGCGATCATTATGGACGGCAGTGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCa                                                                                    <  2:700444/237‑1 (MQ=255)
                                                                                                                                                      aaCCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGAATTGAGGCGTTAACGCTGTATGCCTTTAGTCGGTAAAACTGGAACCGACCAGCGCAGGAAGTACGGGCGTTaaag                                                                         >  1:647057/1‑237 (MQ=255)
                                                                                                                                                        ccACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCACCAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACCGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGa                                                                       >  2:88302/1‑239 (MQ=255)
                                                                                                                                                        ccACTTAGCCAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGGGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGa                                                                       <  2:602285/239‑1 (MQ=255)
                                                                                                                                                         cctttgggaaaaatTTGCCCGCCCAGGGCTCCCGTTATGTGGCGATCATTAGGGACGGCAATGGCCGCTGGGAAAAAAAGCAAGGGAAGATTCGTGCCTTGGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGaa                                                                      <  1:105436/230‑1 (MQ=255)
                                                                                                                                                         cACTTAGCGAAAAATTGCCAGCGCATGGCTTCCGTCATGTTGCGATCATTAGGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGaa                                                                      <  2:671720/239‑1 (MQ=255)
                                                                                                                                                         cACTTAGCGAAAAATGGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGaa                                                                      <  2:881594/239‑1 (MQ=255)
                                                                                                                                                         aaCTTAGCGAAAAATTGCCAGCGCCGGGATGCCGACAGGTTGCGATCATTATGGCCGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGaa                                                                      <  2:467339/238‑1 (MQ=255)
                                                                                                                                                           ctcaGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACt                                                                    >  2:111910/4‑239 (MQ=255)
                                                                                                                                                              aGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGGTTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTCGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTggt                                                                 >  1:607391/1‑237 (MQ=255)
                                                                                                                                                                      aTTGCCAGCCCATGGCTGCCGTCATGTTGCGATCATTATGGCCGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTggg                                                         <  2:522204/239‑1 (MQ=255)
                                                                                                                                                                                    ggcgttcGTTATGTTGGGATCATTATGGGCGGAAATGGCCGCGGGGAAAAAAAGCAAGGGAAGTTTCGTGCCTTTGGGCCAAAAGCCGGGGCAAAATCCGGCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGa                                            <  1:512422/233‑1 (MQ=255)
                                                                                                                                                                                    ggCTGCCGTCACGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGa                                            >  2:54543/1‑239 (MQ=255)
                                                                                                                                                                                          cGTCAGGTTGGGATCATTAGGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTaa                                       <  2:563326/238‑1 (MQ=255)
                                                                                                                                                                                                 tttggttaaTTTTGGACGGCAATGGCCGCTGGGAAAAAAAGCAAGGGAAGTTTCGTGCCTTTGGGGTTAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTg                               <  1:654287/231‑1 (MQ=255)
                                                                                                                                                                                                  aagggtataagaggcaggCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCAAAAAGCCGGGGCAAAATCGGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTg                               <  2:728058/222‑1 (MQ=255)
                                                                                                                                                                                                   gctttctttggtgcgttaaggggCCTCTGGGCAAAAGGGGAAGGGAAGGTTGGGGCGTTTGGTCAAAAAGGCGGGGCAAATGCGGGCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTCGGGCGTTAACGCTGGATGCCTTTAGTAGTGAAAGCTGGAACCGACCAGCGGAGGAAGTCCGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGct                             <  1:3002/218‑2 (MQ=255)
                                                                                                                                                                                                   gccatcttttgggACGGCATTGGCCGTGGGGAAAAAAAGCAAGGGAAGTTTCGTGCTTTTGGGCAAAAAGCCGGGGCAAAACCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCa                             <  1:242383/228‑1 (MQ=255)
                                                                                                                                                                                                      aTCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGAATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGGGCTCGATAGCGAAGTAAAAAGTCTGCAcc                           >  2:608335/1‑238 (MQ=255)
                                                                                                                                                                                                        ctttttggcggggaagggCCGGTGGGGAAAAAAGAAAGGGAAGAGTCGGGCCGTTGGGAAAAAAGCGGGGGAAAAATCCGCCCGCCGGGCTTTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGa                         <  1:424283/222‑1 (MQ=255)
                                                                                                                                                                                                         tttctgaccGGCAATGGCCCTGGGGCAAAAAAGCAAGGGAACATTGGTCCCTTTGGCCATAAGGCCGGGGAAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAATAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGGGAAAACTGGAACCGACCAGCGTAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACa                       <  1:762425/231‑1 (MQ=255)
                                                                                                                                                                                                          ttttggcgggCATTGGCCGCTGGTAAAAAGAACAGTGAAAGTTTCGGTCCTTTGGTCATAATGCCGGTGCCAAAACCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACACCGGTATTGAGGCGTTAACTCTGTATGCCTTTAGTAGGGAATACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACa                       <  1:401771/231‑1 (MQ=255)
                                                                                                                                                                                                           tATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGAATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTTTGTGGCCGCTCGATAGCGAAGGAAAAAGACTgacccgcaata                     >  2:312711/1‑229 (MQ=255)
                                                                                                                                                                                                              ggACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACg                  >  2:46431/1‑239 (MQ=255)
                                                                                                                                                                                                                aCGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGCTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAAATGTTTGTGTGGGAGCTCGATAGCGAAGTCAAAAGTCTGCACCGCCATAACGt                 >  2:570786/1‑238 (MQ=255)
                                                                                                                                                                                                                  ggCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAg                                               >  2:340325/1‑206 (MQ=255)
                                                                                                                                                                                                                   gCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTTTGTGGGCGCTCGATAGCGAAGGAAAAAGTCTGAACCGCCATAACGGGCg              >  2:699922/1‑238 (MQ=255)
                                                                                                                                                                                                                   gCAATGGCCGCTGGGAAAAAAGCCAAGGGAAGGTTTGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGt             <  1:61182/239‑1 (MQ=255)
                                                                                                                                                                                                                    aaagggCCGTTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGt             <  2:615949/234‑1 (MQ=255)
                                                                                                                                                                                                                     attGGCCGTTGGGAAAAAAAGCAAGGGAAGATTCGTTCCTTTGGGCATAAAGCCGGGGCAAAACCCGTCCGCCGGGCTGTCTCTTTTGCGGCAAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTc            <  1:621384/236‑1 (MQ=255)
                                                                                                                                                                                                                     aaTGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCAAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTACTGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGACTGCACCGACATACCGTGCgctt           >  2:800979/1‑236 (MQ=255)
                                                                                                                                                                                                                        ggCCGGTGGGCAAAAAAGCAAGGGAAGTTTGGGGCCTTGGGGCAAAAAGCCGGGGAAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCg        <  1:111910/239‑1 (MQ=255)
                                                                                                                                                                                                                          gcccgtGGGGAAAAAAACAACGGGAAGATTCGTGCCTTTGGGAATAAACCCGGGGAAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCg        <  1:762918/234‑1 (MQ=255)
                                                                                                                                                                                                                              gtGGGAAAAAAAGCAAGGGAAGGTTCGTTCTTTTGGGCATAACGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGtatta   <  1:30253/238‑1 (MQ=255)
                                                                                                                                                                                                                               tgagCAAAAAAGCAAGGGCAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCATTCGTAGTGAAAACTGGAACCGACCAGCGCAGGACGTCAGTGCGCTAATGGACCTGTTTGGGTGGGCGCTCGATAGCGACGTAACAAGACTGAACCGAAAAAACGTGAGtgtgggagttat  >  1:551155/4‑231 (MQ=255)
                                                                                                                                                                                                                               tGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGACGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGCAGTCAGTGCGTTAATGGACCTGTTTGTAGGGGCGCGCGAAAGCGAAGTAAAAAAACTGCACCGAAATAActatcttttataaacatg  >  1:728058/1‑222 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
ATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAGGAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTGCACCGACATAACGTGCGTCTGCGTATTAT  >  NC_000913/194772‑195231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: