BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,817,476	T→C	13.3%	S268P (TCC→CCC)	yicC →	UPF0701 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,476	0	T	C	13.3%	254.2 / 2.0	109	S268P (TCC→CCC)	yicC	UPF0701 family protein
Reads supporting (aligned to +/- strand): ref base T (47/40); new base C (3/16); total (51/58)
Fisher's exact test for biased strand distribution p-value = 2.46e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATA  >  NC_000913/3817247‑3817710

tGCTCAGGTGCAACGGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTTGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCAt                                                                                                                                                                                                                                       <  2:541734/237‑1 (MQ=255)

ctccatggTGCAAATGTAAAACAACCGTCTGGGCTCGGACCTGGTGTGGCGTGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑GTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCAt                                                                                                                                                                                                                                       <  1:848927/231‑1 (MQ=255)

      agttcAAATGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGa                                                                                                                                                                                                                               <  2:805317/235‑1 (MQ=255)

          cAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGACACAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GCCGAAGTCTATCAATGCCGAACCGACAAACCCCAcccacgacccaaaagt                                                                                                                                                                                                                           >  1:224298/1‑225 (MQ=255)

          cAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑CCTTGC‑GCCGAAGCCTATCAATGCCGAAGTGACAAACTCCGccctgtctcttatccc                                                                                                                                                                                                                           >  2:700070/1‑225 (MQ=255)

           aattGGAAAACCACCCGCGGGGGCAGGAACTGGTTCCGCTGGCCCAACGAATTGACGTTGCCGAAGGACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAgt                                                                                                                                                                                                                           <  2:660911/235‑1 (MQ=255)

            aCTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGCCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAAAAC‑TCTTGC‑TTCGAAGGCTACAAATGCCGAAGTGAAAAACCCCACCAACGAGCTGAAAgggt                                                                                                                                                                                                                         >  1:297571/1‑239 (MQ=255)

             cTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTGGAACAC‑TCTTGG‑GTCGAAGTTTATAAATGCCGAAGGGCAAAACTCCCCCacccggcggaaaatggt                                                                                                                                                                                                                        >  1:25149/1‑223 (MQ=255)

                        ccGTTTGGATCGGGGACGGGTTCTGCTGGCACAACGAATGGACGTTGCCGAAGAACTGGATCCCCTCGAAGGGC‑TCTTAAAAGGACTCTACAACATTCTGGAGAAAAAAGAAGGGGTTG‑GTCGTCGTCTGGATTGTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑GCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGa                                                                                                                                                                                                             <  1:147148/239‑1 (MQ=255)

                        ccGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCCGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGGGTTGATTGGGCAGa                                                                                                                                                                                                             >  2:819930/1‑239 (MQ=255)

                           tCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTAGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAATCTATCAATGCGGAAAGGACAAACACCGCCATCACACTGAAAGGGTTTATTGAACAAAtag                                                                                                                                                                                                          >  1:841105/1‑237 (MQ=255)

                             tGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCCCCCTCGAGCTGAAAGGGTTTATTGGACAGATg‑cgc                                                                                                                                                                                                       >  1:228900/1‑239 (MQ=255)

                             tGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGCCTATCAATGCCGAAGTGACAAACTCCGCCATCAAGCTGAAAGGGTTGATTGAGCAGATg‑cgg                                                                                                                                                                                                       >  2:64633/1‑238 (MQ=255)

                                 gCAGGAACTGGTTCTGGTGGCCCAACGAATTGGAGTTCCCGAAGAACTGGATCGCCTCGAAGGTCATGGCAAAGAGAC‑GTACAATATTCTGAAAAAAAAAGAAGCTGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAg                                                                                                                                                                                                    <  1:384265/238‑1 (MQ=255)

                                 gCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAAAACTGGATCGCCTCGAAGGGCATGGCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAg                                                                                                                                                                                                    <  1:170590/238‑1 (MQ=255)

                                  cgggAACTTGTTCCGCTGGCACAACGAATTGCAGGTGCCAAAGAACTGGATCGCCTGGAAGCGCTTGTCAAAGAGAC‑CTAAAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGc                                                                                                                                                                                                   <  1:868923/236‑1 (MQ=255)

                                  cAGGAACTTGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGc                                                                                                                                                                                                   <  2:516375/238‑1 (MQ=255)

                                  cAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGCAAGTGTTGATTGAGCAGATC‑GCCGAGCa                                                                                                                                                                                                  >  2:651314/1‑239 (MQ=255)

                                   aGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCACTGCCGCAGTGACAAACTCCGCCATCGAGCTGAAAGGGTTGATTGAGAAGAGT‑cgcggacag                                                                                                                                                                                                 >  1:64504/1‑234 (MQ=255)

                                     gAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGCGTTGATTGAGCAGATG‑CGCGAGCAGAt                                                                                                                                                                                               >  2:580706/1‑239 (MQ=255)

                                         gggTTCTGGTGGCACAACAAATTGACGTTGCCGAAGACCTGGATCGCCGCGAAGCGCGTGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATTCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAg                                                                                                                                                                                           <  1:496014/238‑1 (MQ=255)

                                           tgtaaaagagacagaCGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACTC‑TCTTGC‑GTCGAAGTGTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑aa                                                                                                                                                                                        <  2:193634/225‑1 (MQ=255)

                                             tCTGCTGGCACAACGAATTGACGTTGCCGGAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGGC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACa                                                                                                                                                                                      <  2:239519/239‑1 (MQ=255)

                                               tgctgGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCCG‑AACATc                                                                                                                                                                                    >  2:196112/1‑239 (MQ=255)

                                                               tGACTTTTCCGAAGTATGTGATCGCCTCGAAGCGCATGTCAAAGAGGC‑CTAAAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTTGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGc                                                                                                                                                                    <  1:823100/239‑1 (MQ=255)

                                                                    ttGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCTAAGGCTATCAATGCCGAAGTGACACACTCGCCCATAGAGCTGAAAGGGTTGTTTGACCAAATG‑CGCGGGCAGA‑TCAGCAACATCAAAAAAccggctcctgtaaaa                                                                                                                                                               >  1:193634/1‑225 (MQ=255)

                                                                      gCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACCC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGAGTTGATTGAGCAGATG‑CGCGAGCAAATACAG‑AACAACGACAAAACAGTAACCCCAAATCt                                                                                                                                                             >  1:378156/1‑239 (MQ=255)

                                                                       tcGAACAGCTGTATCGCCTCGAAGCGCGTGGCAAAGAGAC‑CTACTATATTCTTAAGAAAAAAGAAGCGGTTG‑GGCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGGCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGATCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCtt                                                                                                                                                            <  1:35365/238‑1 (MQ=255)

                                                                       ccGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATAAATGGCGAAGTGACAAACCCCGCCATCGGACTGTAAGGGTTGATTTGGCAGATG‑CGCAGACAGATTAAG‑AAAATAAAAAAACACGAAAAGCCaaatatt                                                                                                                                                            >  1:331402/1‑234 (MQ=255)

                                                                                  gATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCg                                                                                                                                                   >  2:671091/1‑237 (MQ=255)

                                                                                       ccTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTcac                                                                                                                                            >  1:639553/1‑239 (MQ=255)

                                                                                       ccTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGGGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTcac                                                                                                                                            >  2:647223/1‑239 (MQ=255)

                                                                                                tcttGTAAAAGAGAC‑CTAAAACATTACGAAAAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTGTAGGATGCAGGATTTCAACCGCGAGTCGAACCC‑TCTTGC‑GTCGAATTCTATCAATGCCGAAGTGACAAACCCCGCCATCGAGCTGAAAGTGTTGATTGATCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTCCTTTTCCGCCTTCACAAATgccgc                                                                                                                                   <  1:819930/236‑1 (MQ=255)

                                                                                                gcATGTCAAAGGAAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACCC‑TCTTGC‑GGCGAATTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATgccgc                                                                                                                                   <  2:252401/239‑1 (MQ=255)

                                                                                                gcATGTCAAAGAGCC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATgcc                                                                                                                                     <  2:554292/237‑1 (MQ=255)

                                                                                                gcATGCAAAAGAGAC‑ATACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTAGGATGCAGGATTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGAAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATgccgc                                                                                                                                   <  2:509349/239‑1 (MQ=255)

                                                                                                 cATGTAAAAGAGCC‑TTAAAACATTATGAAAAAAAAAAAAG‑GGTTGTGGTGTGCGCTGGATTTTAATATGCAGGAGTTCAACCGCGAGTCGACCAC‑TGTTGC‑GTCGAAGTCTAGCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTTATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATACCTCGTAACGCCATTTTTTACTTTTCCGCCTTCACAAATgccgc                                                                                                                                   <  1:104559/238‑1 (MQ=255)

                                                                                                  aaggtaaaaaggacttaaaaatttCTGAAGAAAAAAGAAGGGGTTG‑GTGGGCGGCGGGATTTTATGATGCAGGAGTTCAACCGGGAGTCGAACCC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGGGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATgccgc                                                                                                                                   <  1:196112/216‑1 (MQ=255)

                                                                                                    ttgtaaaggtacttACAACATTCGAAAGAAAAAAGAGGGGGTTT‑GTCTTCGGTTGGATTTTATGATGGAGGAGTTCAACCGCGAGTCGAACCC‑TTTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACGCCGCCATCGAGCTGAAAGTGTTGATTGGGCAGATG‑CGCAAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCAc                                                                                                                                <  1:881145/226‑1 (MQ=255)

                                                                                                            gaC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCCTAACGCCAATTCTTACTTTTCCGCCTTCACaaaacccccaccacaaacaaa                                                                                                                       >  1:794602/1‑221 (MQ=255)

                                                                                                              c‑cTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCCCAAATGCCGCCACTCAACCAGAGc                                                                                                                     >  2:650507/1‑239 (MQ=255)

                                                                                                                cTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGATGTCTTTCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGGGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAAACACGCCACTCAAACaaaccg                                                                                                                    >  1:120473/1‑234 (MQ=255)

                                                                                                                        ttCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCAttttt                                                                                                            >  2:119144/1‑239 (MQ=255)

                                                                                                                         tCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCAtttttt                                                                                                           >  2:461037/1‑238 (MQ=255)

                                                                                                                         tCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCAtttt                                                                                                             >  2:83277/1‑237 (MQ=255)

                                                                                                                         tCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGGGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAATTGGTAAAGCCAATTCTTACTTTTCCGCCTTAACAAATGCCGCCACTCaaaaaaaagggactttttt                                                                                                           >  1:481754/1‑223 (MQ=255)

                                                                                                                         tCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAAACGCGAGCCGCACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCCCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CCCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCAGCCTTCACAAAAGCCGCCACTCAAACAAAACGGCAtttttt                                                                                                           >  2:406718/1‑238 (MQ=255)

                                                                                                                             aagaaAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGGGCAGATTCAG‑AACATCGATGAACCCGTAACGCCAATTCTTACTTTTCTGCCTTCACAAATGCCGCCCCTCAACCAGACCGGCCTTTttcttc                                                                                                        >  2:499731/1‑238 (MQ=255)

                                                                                                                             aagaaAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAAAGCCAATTCTTACTTTTCCGCCTTCACCAATGCCCCCCCTCAAACAGAGCGGCATTTtttttc                                                                                                        >  1:557400/1‑238 (MQ=255)

                                                                                                                             aagaaAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGAGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGCATACCTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACa                                                                                                                         >  2:364334/1‑221 (MQ=255)

                                                                                                                                  aaaaGAAGTGGTTG‑GTGGTCGTCTGTGTTTTATAATGCAGGAGTTCAACCGCGAGTCTAACCA‑GTTTGC‑GTCGAAGTCTACCAATGCCGAAGTGACAACCTCCGCCATCGAGTTGAAAGTGTTGATTGAGCATATG‑CTCGACCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCa                                                                                                  <  2:530583/239‑1 (MQ=255)

                                                                                                                                  aaaaGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTAACAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCa                                                                                                  <  2:814269/239‑1 (MQ=255)

                                                                                                                                       aagCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTAACAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACAtt                                                                                             <  2:516829/239‑1 (MQ=255)

                                                                                                                                            gTTG‑GTCGTCGTTGGGATTTTATGATGCAGGAGTTCAACCGGGAGTCGAAACC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCAGCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAAt                                                                                         <  2:491795/238‑1 (MQ=255)

                                                                                                                                                     ctttttgattttttttaggagggAGTTCAACCCCGGGTCAAACCC‑T‑TTGCGGTCAAAGTTTATCAATGCCGAAGTGAAAAACCCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTg                                                                                <  1:461037/218‑1 (MQ=255)

                                                                                                                                                          tGGATTTTATGATGCAGGAGTTCAACCGCGGTGCGAACACGTGTGGC‑G‑CGAAGTCTATCAATGCCGAAGGCACAAACCCCGCAATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCAAAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGc                                                                           <  2:356232/239‑1 (MQ=255)

                                                                                                                                                          ggttttttAGGATGCAGGAGTTCCTCCCGGTGGCGAACAC‑CTTTGC‑GTCGAAGTCTATCAATGCCGAATTAACAACCTCCGCCATCGAGCTGAAAGTTGTTAGTGAGCAGATT‑GGCGGGCAGATGCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGc                                                                           <  1:591236/235‑1 (MQ=255)

                                                                                                                                                            gATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGcc                                                                          >  2:471215/1‑238 (MQ=255)

                                                                                                                                                                 tATGAAGCAGGAGTTCAACCGGGAGTCAAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTcc                                                                    <  2:400153/239‑1 (MQ=255)

                                                                                                                                                                  atgatgCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGAAACATTCAATTCTGTTTTGCGTGCCTGCTCCa                                                                   >  2:647176/1‑239 (MQ=255)

                                                                                                                                                                                cAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGGTTTTGCGATGtt                                                     >  2:379749/1‑239 (MQ=255)

                                                                                                                                                                                cAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGtt                                                     >  2:527769/1‑239 (MQ=255)

                                                                                                                                                                                 cacccggAGTCGAACCC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCCCCATCGAGCTGAAAGTTTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGttt                                                    <  2:297571/233‑1 (MQ=255)

                                                                                                                                                                                        aGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCCAATTTTTCGATGTTTTTTTGcc                                             >  1:723545/1‑239 (MQ=255)

                                                                                                                                                                                         gTCGAACCC‑TTTTGC‑GTGGGAGTTTATAAATGCCGAAGTAACAAACCCCGCCATCGAGCTGAAAGTTGTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACAGCGAATAACGCGTAACGCCAATTCTTTCTTTTCCGCCTTCACAAAGGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGccc                                            <  1:471215/239‑1 (MQ=255)

                                                                                                                                                                                           cGAACAC‑TCTTGC‑TGCGAAGTCTATAAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATGGAGCAGATT‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTCCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAg                                          <  2:64504/239‑1 (MQ=255)

                                                                                                                                                                                              cccc‑tctTGC‑GTGGAATTTTTAAAATGCCGAGGTGACAAACTCCGCCATCGAGCTGAAAGTTTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCGAATTCTTATTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGca                                        <  1:302482/235‑1 (MQ=255)

                                                                                                                                                                                                cctttttGT‑GTGGAATTTTATCAATGCCGAGTGGCCAAACTCCGCCATCGGCCTGAAAGGTTTTATTGAGCAGATG‑CGCAAGCAGATTCAA‑AACATCGAATAACTCGTAACGCCAATTCTTCCTTTTCCGCCTCCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGcaca                                      <  1:172220/234‑1 (MQ=255)

                                                                                                                                                                                                cccttttGG‑GTCGAAGTCTATCAAGTCCGAAGTGAAAAACTCCGCCATCGAGCTGAAAGTGTTGGTTGGGAAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGcaca                                      <  2:771940/234‑1 (MQ=255)

                                                                                                                                                                                                 cccctttggggcGAATTTTACCAAGGCCGAGATGACAACCCCCCGCCTCGAGCTAAAAGTTTTGATTTAGCAGATG‑CGGGAGCAGATTCAG‑AACATCGAATAACTCTTAACGCCAATTCTTACTTTTCCGCCTTCAAAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGcaca                                      <  1:647223/228‑1 (MQ=255)

                                                                                                                                                                                                   tctTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTTCCCAGCACACTg                                   >  2:17848/1‑239 (MQ=255)

                                                                                                                                                                                                      tGC‑GTCGAAGTTTATCAATGCCGAAGTGACAAACCCCCCCAACGAGCTGAAAGTGTTGATTGAGGAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACCCGTAACCCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTgaga                                <  2:481754/239‑1 (MQ=255)

                                                                                                                                                                                                       tgggttgAAGTCTAACAAGGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTgaga                                <  2:228900/233‑1 (MQ=255)

                                                                                                                                                                                                        c‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACCAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCGGCTTTTGCGATGTTTTTTTGCCCAGccacctgataac                              >  2:121894/1‑228 (MQ=255)

                                                                                                                                                                                                              aaGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGCGAACGTgag                         >  2:34977/1‑239 (MQ=255)

                                                                                                                                                                                                              aaGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAAAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTTCGTCCCTGCTCCAGGTTTTGCGTTGTTTTTTTCCCCAGCacaccggaaaaggtgg                         >  1:863653/1‑227 (MQ=255)

                                                                                                                                                                                                               aGTCTGCCAATGCCGAAGTGACAACCCCCGACATCGAGCTGAAAGTGTTGATGGAGCAGATG‑CGCGAGCAGATCCAG‑AACATCGAATAACTCTTCACGCCAATTCTTACTTTACCGCCTTAACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCAAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTgag                         <  2:285311/238‑1 (MQ=255)

                                                                                                                                                                                                                     gaaagaccaaaGTGACAAACTCCGACATCGATCGTAAAGTGTTGATTGAGAAGATG‑CGCGAGCAGATTCAA‑AACATCGAATAACCCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTca                  <  1:34977/230‑1 (MQ=255)

                                                                                                                                                                                                                     caaaTGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGGGTTGATTGAGCAGATG‑CGCGAGCAGATTCAA‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTca                  <  2:863653/237‑1 (MQ=255)

                                                                                                                                                                                                                     acaatcccgaatggcaaaaCCCCTCCATCGACGTGAAAGTGTTGATTGGGCAGTGG‑CGCGAGCAGATTCAA‑AACATCGAATACCTCGTAACGCCAATTCTTCCTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTca                  <  1:17848/223‑1 (MQ=255)

                                                                                                                                                                                                                     acAAGGCCGAAGGGACAAACCCCCCCAGCGAGCTGAAAGTGTTGATTGCGCAGGTG‑CGCGAACAGATTCAG‑AACATCGAAAAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTca                  <  1:527769/238‑1 (MQ=255)

                                                                                                                                                                                                                        atcacGAAGTGAAAAACCCCCCCAGCGAGGTGAAAGTTTTGATGGCAGAGATG‑CGGGGACAGATTAAA‑AAAAGAGAATACCGCGTAACGCCAAGTCTTACTTTCCCGCCTTCACAAATGCCGACACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACaa               <  1:121894/235‑1 (MQ=255)

                                                                                                                                                                                                                        aTGCCGAAGTGACAACCCCCCCCATGGAGCCAAAGGTTTTGTTTGAGC‑GACGCCGGGTGCATATTCAG‑AACATCTAATTACTCGTAACGCCAATTCTTACTTTTCCGCCTACACACAGCCCACCACCCAAACAGTGCGGCATTTTTCTGCCCCGGAACACTCAATTCTGATTTGCGGGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACCCTGAGTACGTGAGATACTCCCaa               <  1:406718/239‑1 (MQ=255)

                                                                                                                                                                                                                        aTGCCGAAGTGAAAAACTCCGGCATCGAGCTGAAAGTGTTTATTGAGCAGATG‑CGCGAGCAGTTTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACaa               <  2:378156/239‑1 (MQ=255)

                                                                                                                                                                                                                           cccaggTGAAAAACCCCCCCTACGAGGTAAAAGGTTTTATTGACAAGATG‑CGCAACCCGATTCAG‑AACATCGAATACCTCGTACCGCCAATTCTTACTTTCCCGCCTTCCAAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg            <  1:499731/234‑1 (MQ=255)

                                                                                                                                                                                                                            caaaGTGACAACCCCCGCCTTCGAGCTGAAATGTTTGATTGAGAAGATG‑GCCGAGCAGATTCAG‑AACATCGAATACCTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg            <  2:557400/236‑1 (MQ=255)

                                                                                                                                                                                                                            cGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg            <  2:639553/238‑1 (MQ=255)

                                                                                                                                                                                                                            cGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCACGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGGGAGATACTCCCAACTGt           >  2:513773/1‑239 (MQ=255)

                                                                                                                                                                                                                            cGAAGTGAAAAACCCCGCCAGCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg            <  2:686023/238‑1 (MQ=255)

                                                                                                                                                                                                                             gAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTcccaccggaa          >  2:274270/1‑233 (MQ=255)

                                                                                                                                                                                                                               aGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCaaa                                                                                                                           <  2:182815/124‑1 (MQ=255)

                                                                                                                                                                                                                               aGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCaaa                                                                                                                           >  1:182815/1‑124 (MQ=255)

                                                                                                                                                                                                                               aGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACAtt                                                                                             >  1:827909/1‑154 (MQ=255)

                                                                                                                                                                                                                               aGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACAtt                                                                                             <  2:827909/154‑1 (MQ=255)

                                                                                                                                                                                                                               aGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGtata        >  2:764268/1‑239 (MQ=255)

                                                                                                                                                                                                                                   gcAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGGTCTTCCCCACTGTCTATaaa    >  2:799849/2‑239 (MQ=255)

                                                                                                                                                                                                                                   aCAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATaaa    >  2:561418/1‑239 (MQ=255)

                                                                                                                                                                                                                                    cAAACCCCGCCATCGGGCTGAAAGTTTTGATTGAGCAGATG‑CGCGAGCAGATTCAA‑AACAGCAAATAACCCGTAACGCCAATTCTTACTTTTCCGCCTTCAAAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATaaa    <  1:441420/238‑1 (MQ=255)

                                                                                                                                                                                                                                    cAAACCCCGCCATCGAGCTGAAAGTGTTGGTTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATaaa    <  2:224298/238‑1 (MQ=255)

                                                                                                                                                                                                                                     aaaCTCCGCCATCGAGCTGAAAGTGTTGATTGGGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACGCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATa  <  2:877535/239‑1 (MQ=255)

TGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAC‑CTACAACATTCTGAAGAAAAAAGAAGCGGTTG‑GTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACAC‑TCTTGC‑GTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATG‑CGCGAGCAGATTCAG‑AACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATA  >  NC_000913/3817247‑3817710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`