Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,854,897 A→G 21.5% E326G (GAG→GGG)  emrD → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,854,8970AG21.5% 99.9 / 5.5 59E326G (GAG→GGG) emrDmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (25/19);  new base G (7/7);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGTCATC  >  NC_000913/3854663‑3855131
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               
tGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTAGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGCTGAATGTCTGGACGCTGCTCGTTCACGCCGCGCTGTTGTTTTTCGGTGTCGGGTGGCTGTTTCCGTGGCCGCCCAGCGGCGCTCTGGAGGCg                                                                                                                                                                                                                                             >  2:380574/1‑239 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGGTCCCCGGCTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGGGCCGGGATGCTTTTTCCGCTGGCGACCAGCGGGGCGATGGGGCCGTTcc                                                                                                                                                                                                                                         >  1:153997/1‑238 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCCTTcc                                                                                                                                                                                                                                         >  2:482989/1‑238 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTAGGTGCCGGGCTGCTGTTTCTGATGTCGACCAGCGGGGCGATGGAGCGGTTc                                                                                                                                                                                                                                          >  2:8366/1‑237 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACCCGGCTCGTTCCAGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCCGTTTCCGCCGGCGACCAGCGGCGCGATGGGGCCGGTcc                                                                                                                                                                                                                                         >  2:317987/1‑238 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGCCTGGACGCTGCTCGTTCCCGCCGCCCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCCTGCGACCAGCGGCGCGATGTGGCCGGTcc                                                                                                                                                                                                                                         >  2:691695/1‑238 (MQ=255)
         tGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCACTGGCGACCAGCGGCGCGGTGGGGCCGTTCCCCtt                                                                                                                                                                                                                                     >  2:83606/1‑238 (MQ=255)
                     cAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGCCGCTGCTCTTTCCCGCCGCGCGCTTCTTTTTCGGGGCCGGGTTGGTTTTTCCGCTGGCGCCCACGGGCCGCCTGGCGGCGTTCCCCCTTCTGGCAGGGACg                                                                                                                                                                                                                       >  1:362138/1‑239 (MQ=255)
                                    cATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCCCGGCTGGCGCGCCggcg                                                                                                                                                                                                       >  2:312029/1‑237 (MQ=255)
                                        gTTTGCCGTCCTTCCCAATAACCCTTTCTCCACGTTAATGGGGCAGGCGGTTATCGTCTGCCTGCTGGCTGGCTTGCGGATGGGGATCCCCGCGTGGTTTGGCGTGATGATGGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTcggcg                                                                                                                                                                                                   <  1:153064/239‑1 (MQ=255)
                                         tttGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTcggcg                                                                                                                                                                                                   <  2:743388/238‑1 (MQ=255)
                                            gCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGCTCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCCGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGCCCAGCGGCGCGGTGGAGCCGTTCCCCTGCCCGGCGGGGCAGGCCGGCGCGCGGGGcggggggcg                                                                                                                                                                                               >  1:743388/1‑236 (MQ=255)
                                                     cccAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCTGGAGGCCGTTTCCGCTGGCGCCCAGCGGGGCGCTGGGGCCGTTCCCCTTCCTGGCGGTCACGGCGGGCGGGCTGGGcggcggggtcccacaaac                                                                                                                                                                                      >  1:605098/1‑227 (MQ=255)
                                                               gCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGTTGGAGCCGTTCCCCTTCCTGGCGGGCCCGGCGGGCGGGCTGGTCCGCGGCGTGCCAAAACTTGGGTCCgg                                                                                                                                                                             >  2:247584/1‑238 (MQ=255)
                                                                                   cAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGGGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGCCCAGCGGGGCGACGGAGCCGTTCCCCTTCCTGGCGGGACCGGCTTGCGCGTCGGTCGGCGGGCTCGAAACAATTGGGTCCCGCGTAGGGGGGTCGcgctcggg                                                                                                                                                       >  1:284874/1‑236 (MQ=255)
                                                                                   cAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATTGGGCCGTTCCCCTTCCTTGCGGGCACGGCTGGCCGCGCGGTCGGGGGGTGGGCAAACCTTGGTTTCgggcggccggggggggcttcctg                                                                                                                                                        >  1:446220/1‑218 (MQ=255)
                                                                                   cAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGTCGGGGCGCGCCGGGCGCCGGGGTGGCGGGTCTCCAAACAATGGTTCCgggcggccggggcgccccccccg                                                                                                                                                        >  1:478499/1‑218 (MQ=255)
                                                                                   cAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGGGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGGGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGATGGGCGGCGGACGGCACAAACTTGGTTCCGGCGTGCTGGCGTCGctccttga                                                                                                                                                       >  2:657056/1‑234 (MQ=255)
                                                                                            ttttggtgcctggTGGCTGTGTGGCTGGTGGGGGTCCCCGACTGGTTTGGGGTGATGAATGTCGGGACGCCGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGcc                                                                                                                                              <  1:154958/233‑1 (MQ=255)
                                                                                                gctgcCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGCTGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGCTGGAGCCGTTCCCCTTCCTGGCGGGCCAGGCTGGCCCGCTGGTCGGCGGGCTGCAAAACATTTGGTTCGGGGGGCTGGCGTCCCGCTCTGCGCTGTTGcccgaa                                                                                                                                          >  2:868981/1‑235 (MQ=255)
                                                                                                gctgcCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTGGGCGGGCTGCAAAACATTGGGTCCGGCGGGCTGGCGGCGCCCTCTGCGGTGTTTCCGCaa                                                                                                                                          >  2:829448/1‑239 (MQ=255)
                                                                                                   gcctgcTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGCCGTGCTGGCGGCGCTCCTCGCGAGGTTGcccgcaacc                                                                                                                                       >  2:287270/1‑232 (MQ=255)
                                                                                                    cctgcTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGTTGGCTGCGCCTTTTCCGATGTTGCCGCAAACCg                                                                                                                                      >  2:244326/1‑239 (MQ=255)
                                                                                                    cctgcTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGGCTGCCAAACATTGGTTCCGGCGGGCTGGCGCCGCCCTCTGCGGTGTTGCCGCAAACCg                                                                                                                                      >  2:154958/1‑239 (MQ=255)
                                                                                                    cctgcTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGGCGGCGGCCTGGCAAACATTTGTTCCGGCGTTCTGGGCTCGGCCTCTGCGATGTTGCCGCAAACCg                                                                                                                                      >  2:542604/1‑239 (MQ=255)
                                                                                                        ctggctggCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGGGCTGGCGGCGCTCACTTCGATGTTGCCGACAACCGGTcc                                                                                                                                  >  2:67232/1‑238 (MQ=255)
                                                                                                          ggcgggctTTCTGGTGGGGACCCCCGACTGGTTTGGCGGTATGAATGTCTGGCCGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAgg                                                                                                                                <  2:820169/239‑1 (MQ=255)
                                                                                                           gttgggttgcggtgttgGTTCCCCGCTTGGTTTTGTGGGATAATGTGTCGACCGCTCCTGGCTCCCGCCGCGCTGTTCTTTTTCGGGGCCGGGATGCTGTTTCCACTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAggg                                                                                                                               <  1:83606/224‑1 (MQ=255)
                                                                                                                     ttaTGTGGATCCCCGAGTGGTTGGGGTTGATGAAGGTCTGGACGGTGCTCGTTCCCGGCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTgggg                                                                                                                     <  2:144904/237‑1 (MQ=255)
                                                                                                                                    aCTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCCCGGCTGGCGCGCTGGTCGGCGGTCTGCACAACATTGGGTCCCGCGTGGTGGCCGCGGCTCCTTCGCTGTTGCCGCCAACCGCGCCGGGGCCCCTGGGGGTGTTGTGGAACTTa                                                                                                      >  1:302841/1‑239 (MQ=255)
                                                                                                                                     cttgTTTGGCTTTTTGGATGTTGGGGCGTTGGTCGTTCCCGGCTGGGTGTTTTTTTTCGGTGCCGGGATGCTGTTTCCGCGGGCGGCCCGCCGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGGGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTaa                                                                                                     <  1:247584/236‑1 (MQ=255)
                                                                                                                                          tttgtgtggtgGATGTCTGTGCGGTGCTGGTTCCCCCCGCGCGGTTCTTTTCCGGTGCCGGGATGCTGTTTCCGGCGGGGACCGGCGGCGCGATGGAGCCGTTCCCCTTCCTGGGGGGCACGGGTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGa                                                                                                <  1:195426/234‑1 (MQ=255)
                                                                                                                                             gCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTCCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGGCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCCCCCTCTCCGCTGTTGCCGCAAACCGGTCATGGCGCCCGGGGGGTGTTTGGGAGCTTAAAGGGGttg                                                                                             >  1:491766/1‑239 (MQ=255)
                                                                                                                                               ggtagaaaTGTTTGGGCGCTGCGCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGAttgt                                                                                            <  2:170054/232‑1 (MQ=255)
                                                                                                                                                 gatgaATGTCTGGACGCTGCTCGTTCCCGCCGCGCGGTTCTTTTTCGGTGCCGTGATGCTGTTTCCGCTTGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGa                                                                                         <  2:151333/239‑1 (MQ=255)
                                                                                                                                                  atgaATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTGCCTATCTGCGGTGTTTCCGCAAACCGGTCAGGGCAGCCTGGGGGTGTTGGTGGCCTTAATGGGGatgttatt                                                                                        >  2:415676/1‑236 (MQ=255)
                                                                                                                                                       atgtcggggcgtttgcagTTCCCGACGCGGTGTGCTTTTGCGGTGCGGGGGTGCTGTTACCGGTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCgtg                                                                                    <  2:565465/222‑1 (MQ=255)
                                                                                                                                                                cGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTTCAAAACATTTGTTCCGGCGGGCTGGCGGCGCTCTCTGCGGTGTTTGCGCAAACCGGTCAGGGGAGCCTGGGGGTGTTGGTGGCCTTAATGGGAgtggtggaaggggggtggggg                                                                           >  1:876291/1‑224 (MQ=255)
                                                                                                                                                                        gTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGGCGGCGGTCTGCAAAACATTGGTTCCGGCGGGCTGGCGTCGCTCTCTGCGGTGTTGCCGCAAACCGGGCAGGGCCGGCCGGGGTTGGTGATGCCCTTTATGGGAGTGTTTATCGGGCTGTgctggcgggcggc                                                                   >  1:431370/1‑232 (MQ=255)
                                                                                                                                                                                 gcgcTGTTCTTTGTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGAcgcg                                                        <  2:775740/239‑1 (MQ=255)
                                                                                                                                                                                    cTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGCCCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGGCGGGGGTCTGCAAACCATTGGTTCCCGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGGCAGGGCAGCCCGGGGTTGATGATGGCCTTAATGGGATTGTTGTATGTGCCGGTCTGGCTCCCCCGCGCGACCCggga                                                     >  1:300966/1‑237 (MQ=255)
                                                                                                                                                                                      gtttcttttgggttccggtgttgtGTTTCCGCTGGCGAGCAGCGGCGCGTTGGGCGCGCTCCCCTGCCTGGGGGGCACGGCTGGCGGGGTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCTGCAAACCGTGCAGGGCAGCCTGGGGTTGTTGATGGCCTTAATGGTATTGTTGATCTTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGt                                                   <  1:8366/216‑1 (MQ=255)
                                                                                                                                                                                       ttCTTTTTCGGTGCCGGGATGCTGTTTCCGCCGGCGACCAGCGGCGCGATGGGGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTTAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTc                                                  <  2:284874/239‑1 (MQ=255)
                                                                                                                                                                                        tCTTTTTCGGGGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTc                                                  <  2:44696/238‑1 (MQ=255)
                                                                                                                                                                                           ttttCGGTGCCGGGATGCTGTTTCCGCTGGGCGCCAGCGGCGCGATGGGGCCGTTCCCCTTCCGGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCAt                                              <  2:889479/239‑1 (MQ=255)
                                                                                                                                                                                            tttCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCCCGGCTGGCGCGCTGGGCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGGCAGGGGCGCCTGGGGTTGTTGATGACCTTAATGGGGTTGTTGATCTGGCTGGGCGGGGTGCCGCGGGGGACGCGGGGGTCGccac                                             >  1:829415/1‑236 (MQ=255)
                                                                                                                                                                                                ggctccGGGATGGTGTTTCCGCTGGCGTCCAGCGGCGCGATGGAGCCGTTCCCCTTCCGGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCGCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAggg                                         <  2:478499/235‑1 (MQ=255)
                                                                                                                                                                                                 gTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGGCGGCGGGCCGCAAAACATTGGTCCCGGCGTGCTGGCGGCCCCCTCTGCGCTGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGATGTTGATGACCTTAATGGGATTGTTGAACGTGCTCTTCCGGCTCCCGCTGGCGACCGCGGTGCCGCATCAggtg                                        >  1:190860/1‑237 (MQ=255)
                                                                                                                                                                                                  tGCCGGGATGCTGTTGCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAgggg                                        <  2:302841/238‑1 (MQ=255)
                                                                                                                                                                                                           gCTGTTTCCGCGGGCGACCAGCGGCGCGATGGGGCCGTTCCCCTTCCGGGCGGGCACGGCTGGGGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGttt                              <  2:834670/239‑1 (MQ=255)
                                                                                                                                                                                                            gcgTTTCCGGTGGCGACCAGCGGCCCGATGGAGCCGTTCCCCTTCCGGGCGGGCACGGCTGGGGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGttt                              <  2:829415/236‑1 (MQ=255)
                                                                                                                                                                                                                 tCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGGGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACCCGGATGTCGCATCAGGGGCAGCCCGTTTAAgcg                         >  2:402157/1‑238 (MQ=255)
                                                                                                                                                                                                                      tGGCGGCCGGGGGCGCGATGGAGCCGTTCCCCTGCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGt                    <  2:331729/238‑1 (MQ=255)
                                                                                                                                                                                                                           aCCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTTGCGCGCTGGTCGGCGGGCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATTGTGCCGCAAACCGGGCCGGGCAGCCTGGGGTTCGTGATGACCTTAAAGCGGTTGTTGTTGGTGCGTGGCTGGCTGCCGGTGGCGGACGGGGGTGTGCACTAGGGGAGGCCCGGTTaaagggcaggtccgcg              >  1:788595/1‑225 (MQ=255)
                                                                                                                                                                                                                               ggggcgCGATGGGGCCGTTCCCCTTCCGGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCTTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCgcagca          <  2:167678/237‑1 (MQ=255)
                                                                                                                                                                                                                                       aTGGGGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGtcat   <  2:209070/238‑1 (MQ=255)
                                                                                                                                                                                                                                        tGGGGCCTTTCCCCTTCCTGGCGGGCACGGCTGGCGCCCTGGTTGGCGGGCTGCAAAGCTTTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGtcatc  <  2:219650/238‑1 (MQ=255)
                                                                                                                                                                                                                                        tGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGGTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGtcatc  <  2:459232/238‑1 (MQ=255)
                                                                                                                                                                                                                                        tGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGtcatc  <  2:604507/238‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               
TGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGTCATC  >  NC_000913/3854663‑3855131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: