Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,087,250 T→G 19.8% C84G (TGC→GGC)  yiiG → DUF3829 family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,087,2500TG19.8% 120.9 / 2.9 61C84G (TGC→GGC) yiiGDUF3829 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (20/24);  new base G (10/3);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 6.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGATGGCGTGGCGGTGAGTTATATCGATGCTGCCGTGGCGTTGGG  >  NC_000913/4087018‑4087488
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                
tgtttccctttttatAGTCGCCTCTATTCCCCTCGGTCTGCCGGGTTGTGATGAAAAAAAGCCCAAAACAGAACCTCTCCCGCCTGCCAATGGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTTAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTaca                                                                                                                                                                                                                                            <  1:304282/228‑1 (MQ=255)
  cTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGTGGGTCGAGAAACAAGCGGTTTTCGACGAAAAAAAGGATTTCTAAACAAAGAGCTacaaa                                                                                                                                                                                                                                          >  1:386698/1‑238 (MQ=255)
      cGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAACGGTTTTTGCCGAAAAAAAGGATGTCTAAATAAAGGGATACAAAAAGt                                                                                                                                                                                                                                      >  1:145711/1‑239 (MQ=255)
        cTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTCCTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACACGCGCAACCGGCGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGAAGTCTAAATCAAGGGCCAAAAAAAATTa                                                                                                                                                                                                                                    >  1:289633/1‑239 (MQ=255)
         tattaacggccccATCATTTCCCGCGCTCCGACGGGTAGTAAAGAAAAAAAAGCAAAAAAAGAAACGCTCCCGCCTGCCAATAGCCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTa                                                                                                                                                                                                                                    <  1:153370/227‑1 (MQ=255)
           ttatAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGAGGACTATATCAAGAACTAcaaaaaaacacag                                                                                                                                                                                                                                 >  1:816157/1‑232 (MQ=255)
                   gCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAACCCTGAAACACCTGCGCAACCGTGGGTCGATGAACAAGCGGTTTTCGAAGAAAAAATGGAGTTTTATATAAAGGGCTAAAAAAAGTTAAAAAACCCgga                                                                                                                                                                                                                         >  1:661824/1‑238 (MQ=255)
                   gCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCgg                                                                                                                                                                                                                          >  2:670483/1‑238 (MQ=255)
                   gCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGt                                                                                                                                                                                                                         >  2:286123/1‑239 (MQ=255)
                   gCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGAGGTTTTCGACGAAAAAATGGGTGTCTAAATCAAGGGCTAAACAAAATTAAAGAACCCggg                                                                                                                                                                                                                         >  1:510328/1‑238 (MQ=255)
                     catcatGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGCAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATTTCAAGGGCTACAACAAGTTACAGATCCCGGTAc                                                                                                                                                                                                                       >  2:838887/1‑239 (MQ=255)
                     catcatGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGACCAAGCGGCTTTCGACGAAAAAATGGATGTCTATATCAAGGGCTAAAACAAGTTACAGATCCCGGTa                                                                                                                                                                                                                        >  2:56935/1‑238 (MQ=255)
                     catcatGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAAGGCTAAAACAAGTTACAAAACCCggaaa                                                                                                                                                                                                                       >  1:477143/1‑236 (MQ=255)
                           gTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTCCTACAACAAGTTACAGATCCCGGACCAGCGCa                                                                                                                                                                                                                 >  2:688738/1‑239 (MQ=255)
                             cccggggTCGGGCGGGTTGGGAGAAAAAAAAACCCAAAACAAAAACCCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGt                                                                                                                                                                                                               <  2:508027/234‑1 (MQ=255)
                               cTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAACAGAAGCTAAACCTGAAAAACCTGCGCAACCGTTGGTCGATGAACAAACGGTTTTCTGCGAAAAAAAGGATGTCTATATCAAGAGCTACAACAAGTTAAAGATCCCggaaaaacaaagtgt                                                                                                                                                                                                             >  1:455722/1‑226 (MQ=255)
                                tcggcgcagcGGTTTGTAATGAAAAAAAAGCCGAAACGAAAACGCTCCCGCCTGCCAATAGCTAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTg                                                                                                                                                                                                            <  2:250434/230‑1 (MQ=255)
                                cggggttggggggtgtggaaaaaaaaaagGCCGAAACAAAACCCCCCCCGCCCGCCAATAGTCACCCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTg                                                                                                                                                                                                            <  1:286123/216‑1 (MQ=255)
                                 cGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTa                                                                                                                                                                                                                        >  2:767248/1‑226 (MQ=255)
                                 cGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGGTTTCGACGAAAAAATGGAAGTCTATATCAAGGTCAACAACAAGTAAAAAAACCCGGGACAGCGAAgccggg                                                                                                                                                                                                           >  1:241510/1‑234 (MQ=255)
                                  ggTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGACCAAATCAAGAGCCACAACAAGTTAACGATCCCGGTACAACCCAGGCTGgc                                                                                                                                                                                                          >  1:508027/1‑239 (MQ=255)
                                    gctttcggtttttactataaaaaacCCCAAAACAGAACCCCCCCCCCCTCCCAAATGTCAACCTTCCGCACCATCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCTCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGgcgc                                                                                                                                                                                                        <  1:597134/220‑1 (MQ=255)
                                        aCGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACATGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAAACAGCGGGTTTTGACGAAAAAATGGCTGTCCATTTCAAGGGCTAACACAAGTTAAAAAACCCCGTAAAggacacaacggcgcgtta                                                                                                                                                                                                    >  1:835191/1‑222 (MQ=255)
                                              tgtgATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGACAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGAAGACTGGCGGGTAATGCTg                                                                                                                                                                                               >  2:118178/1‑238 (MQ=255)
                                              tgtgATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCCAGTGCTACAACAAGTTACATATCCCGGAGAAGCGCAGTCTGGCGCGATAGGcgaa                                                                                                                                                                                              >  1:247500/1‑236 (MQ=255)
                                                  atgatAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTg                                                                                                                                                                                           >  2:204406/1‑238 (MQ=255)
                                                                                     gCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAaga                                                                                                                                                       <  2:835191/239‑1 (MQ=255)
                                                                                     gCCAAAAGTCAACCTGCCGCACCAACTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAaga                                                                                                                                                       <  2:510328/239‑1 (MQ=255)
                                                                                      ccAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTACCCTGACTGCCGGAACAATTTTAAACAGGGGCCTCCCGGTCAagaa                                                                                                                                                      >  1:78939/1‑238 (MQ=255)
                                                                                         aTAGGCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCCTCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGt                                                                                                                                                   <  2:455722/239‑1 (MQ=255)
                                                                                          ttacccaCCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTa                                                                                                                                                  <  1:204406/233‑1 (MQ=255)
                                                                                          tAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTa                                                                                                                                                  <  2:386698/239‑1 (MQ=255)
                                                                                          tAGGCAACCGGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTa                                                                                                                                                  <  2:477143/239‑1 (MQ=255)
                                                                                            gTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGACGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTAAagaactaccg                                                                                                                                                >  2:344972/1‑232 (MQ=255)
                                                                                                aCCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGttt                                                                                                                                            >  2:563379/1‑239 (MQ=255)
                                                                                                    gCCGCACCAGGTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATgg                                                                                                                                        <  2:661824/239‑1 (MQ=255)
                                                                                                     ccGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTCCAGCGCAGTCTGGCCCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGc                                                                                                                                       >  2:185989/1‑239 (MQ=255)
                                                                                                     ccGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGAATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACGGGGGCCTACCGGTGAAGGGGCGACTGTTTATGGc                                                                                                                                       >  2:461960/1‑239 (MQ=255)
                                                                                                          ccAGCCCCTGAAGAGAAACCTCCTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTa                                                                                                                                  <  1:398543/239‑1 (MQ=255)
                                                                                                                       cGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGaa                                                                                                                     <  2:555246/239‑1 (MQ=255)
                                                                                                                         aaaCCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATc                                                                                                                   >  2:129221/1‑239 (MQ=255)
                                                                                                                         aaaCCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGGGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATTCTGACTGGCTGAAAGATTTTAAACCGGGGCCAACCGGGGAAGAAGGCAATGTTTATGGCATCTAAGGcctaaaggaatc                                                                                                                   >  1:478160/1‑228 (MQ=255)
                                                                                                                                cTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAACCAGGGGCCTACCGGTGAAGAGCGTa                                                                                                                                                  >  2:299268/1‑201 (MQ=255)
                                                                                                                                cTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTa                                                                                                                                                  <  1:299268/201‑1 (MQ=255)
                                                                                                                                  gAAGCTCCCGTTGAAAAAGAAGAAGCTAAACCTGAAACCCCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGc                                                                                                         <  1:670483/239‑1 (MQ=255)
                                                                                                                                        cccGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAgtg                                                                                                   >  2:422869/1‑239 (MQ=255)
                                                                                                                                                 aaaGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCCAGTGCTCCAACAAGTTACAGATACCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAAAAGGGGCCTACCGGGGAAGAGGGCACTGTTTATGGCATATAAGGGAGTGTTGCACCCAACCTCGCTCAGAGGGAAAAAgg                                                                                          >  1:61903/1‑239 (MQ=255)
                                                                                                                                                  aaGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGc                                                                                         <  2:289633/239‑1 (MQ=255)
                                                                                                                                                              aaCCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTAGGCTGACTGGCTGAAAGAATTTAAACAGGAGCCTACCGGTGCAGAGCGTACTGGTTATGGCATTTTACGCCTTTGGAAATACAACCACGCTGGAGGTGGAAAAGGGGAAAAAAggggt                                                                             >  1:577330/1‑235 (MQ=255)
                                                                                                                                                                           tGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGGACCGTTCATGGCATCTCAGGCATTCGTGAAACACACCTCCTTTGGGGGGAAAAAAGGCGTAAAAAGAGCTGGGGGTTaaac                                                                 >  1:62433/1‑236 (MQ=255)
                                                                                                                                                                                 aCCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTgcgc                                                          <  2:816157/239‑1 (MQ=255)
                                                                                                                                                                                         tcgagaaaaaggCGGTTTTCGACGAAAAAATGGGTGTCTATGTCAAGGGTTACAACAAGTTACAGATCCCGGTCAAGCCCAGGCTGGCGGGTTAAGCTGTCTGGCTGAAAGATTTTAAACAGGGGCCTACCGTTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAAcc                                                   <  1:56935/227‑1 (MQ=255)
                                                                                                                                                                                                  aagggtttttCGACGAAAAAATGGATGTCTATAGCAAGGTCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTTGCTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGAtggc                                         <  1:838887/233‑1 (MQ=255)
                                                                                                                                                                                                         tttCGACGAAAAAATGGATGTCCATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTAAAAGCTTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGAtggcgtggcg                                   <  2:414594/238‑1 (MQ=255)
                                                                                                                                                                                                          ttCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGATGGCGTGGCGGt                                 <  2:861547/239‑1 (MQ=255)
                                                                                                                                                                                                              acgaAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGGGTGTGAAAAAGGCGTAAAAAGGTCTGTGGGGTTAACGCCTGCGCTGCAACCAATTTATggcggggcgggggag                             >  1:861547/1‑234 (MQ=255)
                                                                                                                                                                                                                              cTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGATGGCGTGGCGGTGAGTTATATCGATGCTGCCg            <  2:145711/239‑1 (MQ=255)
                                                                                                                                                                                                                                   ttaaGTGCTATCACAATTTACAGATCCCGGTACAGCGCAGTCTGCCGCTTTATGCTGACTGGGTGAAAGATTGTAAACAGGGGCCTACCGGTGAAGGGCGTTCTGTTTATGGCATCTACGGCATTAGTGAATCCACCCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGATGGCATGGCGGTGAGTTATATCGATGCTGCCGTGGCg       <  1:102875/237‑1 (MQ=255)
                                                                                                                                                                                                                                   taaaGGGCTTCAACCAGTTACAGATCCCGGTACAGCGCAGTGTGGCGCGTTATGCTGAATGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGATGGCGTGGCGGTGAGTTATATCGATGCTGCCGTGGCg       <  1:563379/237‑1 (MQ=255)
                                                                                                                                                                                                                                   tCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACGGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTGCGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGATGGCGTGGCGGTGAGTTATATCGATGCTGCCGTGGCg       <  2:78939/239‑1 (MQ=255)
                                                                                                                                                                                                                                      aGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGAACTGTTTATGGCATCTACCGCATTAGTGAATCCAACCTCGCTGAGGGTGAAAAAGGCGTAAAAAGTGCTTTGGCGTTAACGCCTGCGCTGCCACCAATTGATGGCGTGGCGGTGGAGTTATTCGAGTCCGCCGTGGCCTTg    >  2:786819/1‑239 (MQ=255)
                                                                                                                                                                                                                                        tGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCCATTGATGGCGTGGCGGTGGGTTTTATTCATGCTGCCGTGGCGTTggg  >  2:870330/1‑239 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                
ATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAGAAGCTAAACCTGAAACACCTGCGCAACCGGTGGTCGATGAACAAGCGGTTTTCGACGAAAAAATGGATGTCTATATCAAGTGCTACAACAAGTTACAGATCCCGGTACAGCGCAGTCTGGCGCGTTATGCTGACTGGCTGAAAGATTTTAAACAGGGGCCTACCGGTGAAGAGCGTACTGTTTATGGCATCTACGGCATTAGTGAATCCAACCTCGCTGAGTGTGAAAAAGGCGTAAAAAGTGCTGTGGCGTTAACGCCTGCGCTGCAACCAATTGATGGCGTGGCGGTGAGTTATATCGATGCTGCCGTGGCGTTGGG  >  NC_000913/4087018‑4087488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: