BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,107,633	C→G	8.5%	A28G (GCG→GGG)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,107,633	0	C	G	8.5%	313.0 / 2.9	114	A28G (GCG→GGG)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base C (37/63); new base G (9/2); total (49/65)
Fisher's exact test for biased strand distribution p-value = 7.32e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

CATTTTGTGTATAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTT  >  NC_000913/4107396‑4107866

cATTTTGGGTAAAAAATTCCGCCATTTGGCCGGACCTGAATCAATTCAGCAGGAAGTGATTGTTATTCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTgcg                                                                                                                                                                                                                                                  <  2:736616/239‑1 (MQ=255)

        gtATAAAATTCCGCCATTTGGCCTGAACTGAATCAATTCAGCAGGAAGTTATTGTTATACTATTTTCACATTCGTTGGATCACGTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGa                                                                                                                                                                                                                                          <  2:19789/239‑1 (MQ=255)

        gtATAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGa                                                                                                                                                                                                                                          <  2:662917/239‑1 (MQ=255)

        gtAAAAAATTCCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGa                                                                                                                                                                                                                                          <  2:90942/239‑1 (MQ=255)

                 acctgcATTTGGCCTGACCTGAATCAGTTCAGCAGGAAGTGATTGTTATACTATTTTGCCATTCGTTGGATCACTGCGTTGTGCAAGAATACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGa                                                                                                                                                                                                                                <  2:116095/234‑1 (MQ=255)

                   cGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCCGGCATGAAAGCCCCAGTTCGCGGGGGGGTTGGTTTTGCGCTGAAAAAAgggtgcggag                                                                                                                                                                                                                               >  1:3350/1‑231 (MQ=255)

                   cGCCATTTGGCCTGACCTGAATCAATTCAGAAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGAATTCCTGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGCTTAAGAAAATCGGAG‑TGTTGACAAACGGAGGTGATGCGCCCTGCATGAACGCCGCAATTCTGGGGGGTGTTCGGTCTGAGCTGgcaggaaggca‑ggaag                                                                                                                                                                                                                              >  1:457578/1‑239 (MQ=255)

                     ccATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGGTGTTGGTTCTGCGCTGGCAGAAGGTCT‑GGAAGTa                                                                                                                                                                                                                            >  2:212735/1‑239 (MQ=255)

                       aTTTTGTCTGACCTGAATCAATTCAGGAGGAAGTGTTTTTTATACTATTTGTACATTCGTTGTATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAAt                                                                                                                                                                                                                          <  1:378645/239‑1 (MQ=255)

                        tttGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCATATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGGTGTTCGTTCTGCGCTGACAGAAAGTCT‑GGAAGAAATg                                                                                                                                                                                                                         >  2:347497/1‑239 (MQ=255)

                        tttGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCGATTTGCGGGGGTGTTCGTTCTGCGCTGCCAGAAGGTCT‑GGAAGTAATg                                                                                                                                                                                                                         >  2:821307/1‑239 (MQ=255)

                          tGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCCCAATTCCCGGGGTTTTTCGTTCTGCGCTGGCCCAAGGTCT‑GGCAGTAATggg                                                                                                                                                                                                                       >  2:861941/1‑239 (MQ=255)

                                 acctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGCCAAGCGGCGGGGATGGGCCAGGGCTAACAGCCGCACGTTCCGGGGTGGTTGGGTCTGTGCGGGCAGAAGGGCT‑GGAAGAAAAGGGGATTTAt                                                                                                                                                                                                                >  1:274592/1‑239 (MQ=255)

                                 acctgaATCAATTCAGCAGGAAGCGATGGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGGCTTCCGGCAATAGATATCATTTTGCATTCCAAAGTTCAGAGGGAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGCCGGGGGTGAGCCGGGGCTGCAGGCAGCAAGTCGCGGGGGGGATCGTTGTTAGCTGACAATAGGAAT‑GGGAGGAATGGGTATTTAt                                                                                                                                                                                                                >  1:81505/1‑239 (MQ=255)

                                    tgaATCAATTCAAAAGGAAGTGTTTGTTATACTTTTTGCACATTGGTTTGATCAATTCGAGGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGAc                                                                                                                                                                                                             <  1:749734/239‑1 (MQ=255)

                                      aatagaTTCAGTAGGAAGTGATTGTTTTTCTTTTTGCCAATTTTTTGTCGCACTTCGATGGGCAAGAAGACTTCCGGCAACAGATTTCAGTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACgg                                                                                                                                                                                                           <  1:383565/234‑1 (MQ=255)

                                      aaataaTTCAGGCGGAAGTGATTTTTATACTATTTGCACATTCGTTGGATCCCTTCGATGTGCAAGAAGACTGCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTTGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACgg                                                                                                                                                                                                           <  1:211304/235‑1 (MQ=255)

                                      aaTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGCCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCCCAATTCGCGGGGCTGTTCGCTCTCGGCTGAAAGAAGGTCT‑GTGAGTAATGGGGATTTTTGACg                                                                                                                                                                                                            >  2:383565/1‑238 (MQ=255)

                                       aTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAAt                                                                                                                                                                                                                          <  2:778619/223‑1 (MQ=255)

                                       aTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACgg                                                                                                                                                                                                           <  2:253035/238‑1 (MQ=255)

                                           aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATAGGTG‑TGTTGACAAGCGGCGGAGATGCGCCAGGGATGAAAGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGGAAGAAGGGAT‑CGAAGTAATGGGGATTTATGACggaca                                                                                                                                                                                                        >  1:130707/1‑234 (MQ=255)

                                            ttCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTAGCGGGGTTGTTCGTTCTGCGCGGACAGAAGGTCT‑GGAAGTAATGGGGATTTATGACGGCTATcg                                                                                                                                                                                                     >  2:147120/1‑238 (MQ=255)

                                                gcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGGTGTTCGTTCTGCGCTGGCAAAAGGTCT‑GGAAGTAATGGGGATTTTTGACGGCTATCTgggg                                                                                                                                                                                                 >  2:129658/1‑238 (MQ=255)

                                                 cagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGTGCCAGGCATGAAAGCCGCAATTTGCGGGGTTGTTCGGTTTGGGCTGGCAGAAGGGCTGGGAAGAAATGGG‑ATTTAtagtgtctcttttaaac                                                                                                                                                                                                >  1:778619/1‑223 (MQ=255)

                                                 cagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAAGCCTGAAATCCGCCATTTCCGGGGGTTTTCGGTTTGGCCTGACAGAAGGACT‑GGAAGTAAGGGGGGTTTATGGGGGGAATCTggggg                                                                                                                                                                                                >  1:365343/1‑237 (MQ=255)

                                                  agGAAGTGATTTTTATACTCTTTGCACATTGGTTGGATCCCTGCGAGGGGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTc                                                                                                                                                                                                <  1:98282/238‑1 (MQ=255)

                                                  agGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTc                                                                                                                                                                                                <  2:348051/238‑1 (MQ=255)

                                                  agGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTc                                                                                                                                                                                                <  2:361925/238‑1 (MQ=255)

                                                     aatgtatttttttcctttttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTGCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTc                                                                                                                                                                                                <  1:807919/218‑1 (MQ=255)

                                                     aaGTGATTGTTATTCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTc                                                                                                                                                                                                <  2:703995/235‑1 (MQ=255)

                                                         tttttttttaCTATTTGCCAATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGaa                                                                                                                                                                                        <  1:611410/231‑1 (MQ=255)

                                                         tattttttttttttttGCATTTTCTTTGGATCCCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGCTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGaa                                                                                                                                                                                        <  1:347497/226‑1 (MQ=255)

                                                         gATTGTTATTCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGaa                                                                                                                                                                                        <  2:477908/239‑1 (MQ=255)

                                                         gATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTCCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGaa                                                                                                                                                                                        <  2:266004/239‑1 (MQ=255)

                                                           tttttttaCTTTTTGCACATTCGTTGGTTCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTGCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGa                                                                                                                                                                                      <  1:424123/233‑1 (MQ=255)

                                                           tttttATACTATTTGCACGTTCGTTGGGTCCCTTCTATGTGCAAGAAGTCTTCCGGCAACAGATTTCATTGTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGa                                                                                                                                                                                      <  1:616558/236‑1 (MQ=255)

                                                           ttGTTATTTTTTTTGCCCATTCGTTGGATCCCTTCGATTGGCAAGAAGGCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGGG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGa                                                                                                                                                                                      <  1:147120/239‑1 (MQ=255)

                                                            tGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGa                                                                                                                                                                                      <  2:569900/238‑1 (MQ=255)

                                                               ttttcTATTTGCACATTTGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGt                                                                                                                                                                                  <  1:212735/235‑1 (MQ=255)

                                                                tttctttttGCACTTTCGTTGGATCCCTTCGATGTTGAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAAGCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTa                                                                                                                                                                                 <  1:184983/233‑1 (MQ=255)

                                                                    tATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGGCAGAAGGGCT‑GGGAGTAATGGGTATTTATTACGGCCATTCGGGTCTGTTTTAAGACCCGATGGt                                                                                                                                                                             >  2:179413/1‑239 (MQ=255)

                                                                    tATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGt                                                                                                                                                                             <  2:277114/239‑1 (MQ=255)

                                                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCCATTCGCGGGGTTGTTCGTTCTGCGCTGACAGCAGGGCT‑GGAAGGAATGGGGATTTATGACGGACATCTGGGTCTGTATGAAGACCGTTTGGAAc                                                                                                                                                                           >  2:95222/1‑239 (MQ=255)

                                                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCt                                                                                                                                                                                                                                    <  1:44848/183‑1 (MQ=255)

                                                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGa                                                                                                                                                                                      <  2:711884/228‑1 (MQ=255)

                                                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCGCCATTCGCGGGGGTGTTCGGTCTTCGCTGACAGAAGGACT‑GGGAGTAAAGGGGATTTATGACGGGCATCTGGGGCTGGGTGGAGACCgagagggac                                                                                                                                                                           >  1:2283/1‑231 (MQ=255)

                                                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGCCGGTGATGCGCCACGCATGAACGCCCCAATTCCCGGGGTTGTTCGGTCTGGCCTGACAGAAGTGGT‑GGAAGTAATGGGTCTTTTTTACGGCTGTTGGGGTCTGTATGCAAACCggctgggaa                                                                                                                                                                           >  1:692326/1‑231 (MQ=255)

                                                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGGAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCt                                                                                                                                                                                                                                    >  2:44848/1‑183 (MQ=255)

                                                                        tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTTAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCGCAATTCGGGGGGGTGTTCGTTCTGCGCTGGCAGAAGGTCT‑GGAAGGAATGGGGATTTTTGCCGGCTATTTGGGTCTGTATGAAGACCGGATgggaaa                                                                                                                                                                          >  2:872575/1‑234 (MQ=255)

                                                                        tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGGCAGGCATGAACGCCGCAATTCGCGGGGTTGTTGGTTTTTCGCTGCCAGAAGGGCT‑GGAAGGAATGGGGTTTTTTGTCGGCCATTTGGGGCTgattgagacagcgatggaaaag                                                                                                                                                                         >  1:797366/1‑218 (MQ=255)

                                                                        tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATTTGGGTCTGTATTAAGACCGTATgggaac                                                                                                                                                                          >  2:184983/1‑234 (MQ=255)

                                                                        tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCCGGGGGTTGTTCGTTCTGCGCTGACCGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTTTGagaacccgaggggacag                                                                                                                                                                         >  2:657432/1‑223 (MQ=255)

                                                                        tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCCCGGGGTTGTTCGTTCTTCGCTGACAGAAGGGCT‑GGGAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTaaa                                                                                                                                                                          >  2:608904/1‑236 (MQ=255)

                                                                         gCACATTCGTTGGATCCCGTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGc                                                                                                                                                                        <  2:2283/239‑1 (MQ=255)

                                                                           acaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTACGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTTGCGGGGTTGTTCGTTTTGCGGTGACCGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTTTCTGGGGCTGTATGAAGACCGAATCGTCCACCTa                                                                                                                                                                      >  2:697995/1‑239 (MQ=255)

                                                                             ttgtgTTGGTTCTCTGTGATGTTCAAGAAGGCTTCCGGGAAGTGAGTTCCTTTTGCCTGCCTAAGTGCAGAGGTAGTCCTGATGTAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGGCAGAAGGTCT‑GGAAGTTATGGGTATTTTTGCCGGCTATCCGGGTCTGTATGAAGACCGTATGGTACAGCTAGa                                                                                                                                                                    <  1:254114/235‑1 (MQ=255)

                                                                                 gTTGGAACCCTTCGATTTGCAAGAAGGCTTCCGGCAACAGTTTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGt                                                                                                                                                                <  1:129658/239‑1 (MQ=255)

                                                                                      aTCCCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGc                                                                                                                                                                        <  2:130509/226‑1 (MQ=255)

                                                                                      aTCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGa                                                                                                                                                                                                                                          <  2:91752/161‑1 (MQ=255)

                                                                                          cTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGGTGCGCCCGGCATGAACGCCGCAATTCGCGGGGGTGGTCGTTCTGCGCTTTCCGCAGTGTT‑GGAAGTAAGGGGGTGTTATTTAGGCTATCTGGGTCTGTGTTGAGGACGCATTGGACAGACAGACGGGTACAGCgcg                                                                                                                                                       >  1:216842/1‑237 (MQ=255)

                                                                                             cGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGGCCCAGGCATGAAAGCCGCCATTCGCGGGGGTGGTCGTTTTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGGACTTATTTCGGCTAGCTGGGGCCGTATGAAGACCGGTTGGTAAAGCCAGAACGttgaaagggg                                                                                                                                                       >  1:688898/1‑228 (MQ=255)

                                                                                              gATGTGCAAGAAGACTGCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTg                                                                                                                                                   <  2:173679/239‑1 (MQ=255)

                                                                                                tttgCAAGAAACCTTCCGGCAACAGATTTTATTTTGCATTCCAAAGTTCAGAGGTAGTCATGAATAAGAAAATCGGTG‑GGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGAc                                                                                                                                                 <  1:861941/237‑1 (MQ=255)

                                                                                                ttggaaagaagCCTTCCGGAAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGGCATGATTAAGAAAATCGGTG‑TGGTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGAc                                                                                                                                                 <  2:682403/234‑1 (MQ=255)

                                                                                                tgtgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACCGAAGGGCT‑GGGAGGAATGGGGATTTTTGACGGCTATCCGGGTCTGTATTAAGACGGTATAGGAAAGTTAAACCGTTAcctgtctctttta                                                                                                                                                 >  1:130509/1‑227 (MQ=255)

                                                                                                     aagaagACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGGTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGGAATGGGGATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGGGTCTGACATG‑At                                                                                                                                           >  2:816619/1‑239 (MQ=255)

                                                                                                         agaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAAc                                                                                                                                       <  2:198154/239‑1 (MQ=255)

                                                                                                            cTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCCCGGGGTTTGTCGTTCTGCGCTGACAGAAGGGCT‑GGAAGTAATGGGGAGTTTTTAAGGCTATCTGGGGCTTGATTGAGACCGTATGGGAAAGCATGACCGTTCCacggtgcttttaaatgttaaacag                                                                                                                                     >  1:781522/1‑216 (MQ=255)

                                                                                                               ccGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGGCTGACATG‑ATCAACCGTGGc                                                                                                                                 >  2:649295/1‑239 (MQ=255)

                                                                                                                ccgCAACAGATTTCATTTTGCATTCCAAAGTGTAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCg                                                                                                                                <  1:208436/237‑1 (MQ=255)

                                                                                                                   cAACAGATTTCTTTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTa                                                                                                                             <  1:390720/239‑1 (MQ=255)

                                                                                                                   aaataattttCTTTTGGTATTCAAAAGTTCGTTGGTAGTCATTATTAAGAAAGGCGGGT‑TGTTGACAGGCGGCGGTGATGCGCCAGGCCTGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGTCCGTTACAGCGTGTCTGACATG‑ACCAACGGTGGCGGTa                                                                                                                             <  1:774350/232‑1 (MQ=255)

                                                                                                                   aaaCAGTTTTTATTTTTCCTTCCAAATTTCAGGGGTTGGCATGGTTAAGAAAATCGGTGTTGTTG‑CCAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTa                                                                                                                             <  1:82629/238‑1 (MQ=255)

                                                                                                                     aCAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGGG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGGCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑g                                                                                                                         <  2:532217/239‑1 (MQ=255)

                                                                                                                            ttaTTTTTGATTCCAAAGTTCAGGGGGAGTCATGATTTAGAAAAGCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGTCGCAATTCGTGGGGTGGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCg                                                                                                                  <  1:872575/237‑1 (MQ=255)

                                                                                                                                      tttcaaattgcaggggttggtaTGATTAAGAAAATGGGGG‑GGTTGGACAGCGGCGGGGAGGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGGCAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGcg‑c‑g                                                                                                     <  1:290105/218‑1 (MQ=255)

                                                                                                                                                        gTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCCGGCATGAACGCCGCAATTCGCGGGGGTGTTCGTTCTGCGCTGACAGAAGGGCT‑GGCAGTAATGGGTATTTATGACGGCTATCCGGGTCTGTGTGAAGACCCGATGGTACAACATGAACGTTACAGCGGGGCTGACATGTGACAACCG‑GGCGGGA‑A‑AGTCCCCTGTTC‑TCCG‑G‑GTTTCCCgggacttcctcc                                                                                   >  1:180711/1‑229 (MQ=255)

                                                                                                                                                               ttAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGGAGTAATGGGGGTTTATGACGGCTATCTGGGGCTGTATTAAGACCGGATGGGAAAGCTAGACCGTTAAAACGGGGCTGACCTG‑AACACCCCGGCCGGGA‑A‑CGTCCCCGGGTC‑TGGGAC‑GTTT‑CCGGAAATCcgccaaggaaaa                                                                            >  1:44487/1‑230 (MQ=255)

                                                                                                                                                               ttAAAAAAATCGGTG‑TGTTGACAAGCGGGGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGTGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGaa                                                                             <  1:451559/238‑1 (MQ=255)

                                                                                                                                                                  aGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAAGCATGAACGCCGCAATTCGCGGGGTTGTTCGGTCTGCGCCGACAGAAGGTCT‑GGAAGTAATGGGGATTTATGCCGGCTATCTGGGGCTGGATGAAGAACGTATGGGTCAACATGACCGTTCCAGCGGGTCTGACATG‑ACAAACCGTGGCGGGA‑CGGTTCCCCGTTTG‑TGGG‑C‑GTTTCCC‑GAATTcccccaacagacaaa                                                                          >  1:261243/1‑225 (MQ=255)

                                                                                                                                                                       aTCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGGA‑C‑GTTCCTCGGGTC‑TGCG‑C‑GTTTTCCGGAATTCCGCGCCGGGAACAcccccc                                                                     >  2:581890/1‑235 (MQ=255)

                                                                                                                                                                               tgtTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGGATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GGTTCCCGGAATTCCGCGACGAGAACATCCGCGACGGGGcg                                                             >  2:635761/1‑238 (MQ=255)

                                                                                                                                                                                         cggcggTGATGCGCCAGGCATGAACGCCGCAATTTGCGGGGTTGTTCGGTCTGGGGTTACAGAAGGTCT‑GGAAGGAATGGGGATTTATGACGGCTATCTGGGGCTGTATTAAGACCGTATGGTTCAGCGTGACCGTTACAGCGTGTCTGGACTG‑ATCAACCGTGGCGGTAGC‑GTT‑CTCGGGTC‑TGCG‑CGGTTTCCGGCAATCTCGCG‑CGCGAAAAACCGCGGCGTGGCGAGGG‑AAAAca                                                  >  1:439609/1‑238 (MQ=255)

                                                                                                                                                                                           gcggTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGGTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGGATTTATGACGGCTATCTGGGGCTGTATGGAGACCGTATGGTGCAGCTAGACCGTTACAGCCTGTCTGGCCTG‑ATCAACCGTGGCGGGC‑A‑GTTCCTCGGTTCGTGC‑‑C‑GTTTCCCGGCATTCCCCGCCGAGAGAACCCCCCCCGTGGCGCTCGCAAAAccg                                                 >  1:611562/1‑238 (MQ=255)

                                                                                                                                                                                               gaaTGCCCCAGGCATGAACGCCGCAATTCGGGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGaaa                                             <  2:441784/236‑1 (MQ=255)

                                                                                                                                                                                                   gcgcCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACg                                        <  2:235276/239‑1 (MQ=255)

                                                                                                                                                                                                    cgcCAGGCATGAACGCCGCAATTCGGGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGGAGTAATGGGGATTTATGACGGCTATCTGGGGCTGTATGAAGACCGTATGGTACAGCTAGACCGGTACAGCGCGGCTTGCAGG‑ATCAACCGTGGGCGGA‑C‑GGTCCTCCGTTT‑CGCG‑CGGTTCCCCGGATTACCCCGCGGGGAACATCCGCCCCGGGCGGGTCG‑AAAACCGAaaaaaaag                                        >  1:549387/1‑237 (MQ=255)

                                                                                                                                                                                                         ggCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGGCGGAAACATCCCCCCCGGGGCTCACG‑AAAAACTGaaaaaaagggggaa                                  >  1:234040/1‑231 (MQ=255)

                                                                                                                                                                                                            attaaggccgaatttggggggtTTGTGTGTTCTGGGTGGAAAGAAGGGCT‑GGAAGTAATGGGTATTTATGACGGGTATCGGGGTCTGGATGAAGCCCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑AGCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGa                               <  1:24448/221‑1 (MQ=255)

                                                                                                                                                                                                             tgaactgctaaaTTGGCGGGGTTTTGTGGTTTGGCCTGAAAGAAGGTCT‑GGAATTAAGGGGTATTTATGAGGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGa                               <  1:821307/228‑1 (MQ=255)

                                                                                                                                                                                                                    cgcAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGCCGGGAACATCCGCGCCGGGGCTATCG‑AAAACCTGAAAAAACGTGGGATCGACGCGCtgg                       >  2:180281/1‑239 (MQ=255)

                                                                                                                                                                                                                                    gttcgttcTGCGCTGACGGAAGGTCT‑GGAAGTAATGGGTATTTAGGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGa       <  2:439609/239‑1 (MQ=255)

                                                                                                                                                                                                                                    gttcgttcTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑CAAACCTGAAAAAACGGGGTATCGACGCGCTGGTGGTTATcggcggggg       >  2:4949/1‑236 (MQ=255)

                                                                                                                                                                                                                                    gttcgttcTGCGCTGACAGAAGGCTT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGa       <  2:793725/239‑1 (MQ=255)

                                                                                                                                                                                                                                     ttcgttcTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCCCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGGGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGATCCCCCGGGGGTTATTGGGGGTgtc      >  2:133712/1‑237 (MQ=255)

                                                                                                                                                                                                                                      tcgttcTGCGCTTGCAGAAGGTCT‑GGAAGTAATTGGTATTTATGACGGCTATCTGGGTTTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACg     <  2:797366/239‑1 (MQ=255)

                                                                                                                                                                                                                                        gttcTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTGATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGt   <  2:3350/239‑1 (MQ=255)

                                                                                                                                                                                                                                          tcTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGCCGTGAACATCCGCGCCTTGGCTATCG‑CAAACCTGAAAAAACGTGGTATCGACGCGCTGGGGGTTATcggcgggtggaggt  >  2:243913/1‑230 (MQ=255)

                                                                                                                                                                                                                                              cgcTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGa                                                                                                                                                                                      >  2:421189/1‑61 (MQ=255)

                                                                                                                                                                                                                                              cgcTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGa                                                                                                                                                                                      <  1:421189/61‑1 (MQ=255)

CATTTTGTGTATAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTG‑TGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCT‑GGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATG‑ATCAACCGTGGCGGTA‑C‑GTTCCTCGGTTC‑TGCG‑C‑GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCG‑AAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTT  >  NC_000913/4107396‑4107866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`